Abstract:
:Fluorescence diagnostic techniques are notable amongst many other optical methods because they offer high sensitivity and noninvasive measurement of tissue properties. However, a combination of multiple scattering and physical heterogeneity of biological tissues hampers interpretation of the fluorescence measurements. Analyses of the spatial distribution of endogenous and exogenous fluorophores excitation within tissues and their contribution to the detected signal localization are essential for many applications. We have developed a novel Monte Carlo technique that gives a graphical perception of how the excitation and fluorescence detected signal are localized in tissues. Our model takes into account the spatial distribution of fluorophores, the variation of concentrations and quantum yield. We demonstrate that matching the refractive indices of the ambient medium and topical skin layer improves spatial localization of the detected fluorescence signal within the tissues.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Churmakov DY,Meglinski IV,Greenhalgh DAdoi
10.1117/1.1645796subject
Has Abstractpub_date
2004-03-01 00:00:00pages
339-46issue
2eissn
1083-3668issn
1560-2281journal_volume
9pub_type
杂志文章abstract::Selective photothermal interaction using dye enhancement has proven to be effective in minimizing surrounding tissue damage and delivering energy to target tissue. During laser irradiation, the process of photon absorption and thermal energy diffusion in the target tissue and its surrounding tissue are crucial. Such i...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2204615
更新日期:2006-05-01 00:00:00
abstract::Ambulatory diffuse optical tomography (aDOT) is based on near-infrared spectroscopy (NIRS) and enables three-dimensional imaging of regional hemodynamics and oxygen consumption during a person’s normal activities. Although NIRS has been previously used for muscle assessment, it has been notably limited in terms of the...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.9.091314
更新日期:2016-09-01 00:00:00
abstract::Fluorescence image-guided surgery (FIGS) is an emerging technique in oncology, neurology, and cardiology. To adapt intraoperative imaging for various surgical applications, increasingly flexible and compact FIGS instruments are necessary. We present a compact, portable FIGS system and demonstrate its use in cardiovasc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3378128
更新日期:2010-03-01 00:00:00
abstract::We demonstrate the in vivo assessment of human scars by parametric imaging of birefringence using polarization-sensitive optical coherence tomography (PS-OCT). Such in vivo assessment is subject to artifacts in the detected birefringence caused by scattering from blood vessels. To reduce these artifacts, we preprocess...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.12.126014
更新日期:2014-12-01 00:00:00
abstract::Recaldent is a product of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP). The remineralizing potential of CPP-ACP per se, or when combined with 0.22% Fl gel on artificially demineralized enamel using laser florescence, is investigated. Mesial surfaces of 15 sound human molars are tested using a He-Cd lase...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3210780
更新日期:2009-07-01 00:00:00
abstract::Fluorescence lifetime imaging (FLi) could potentially improve exogenous near-infrared (NIR) fluorescence imaging, because it offers the capability of discriminating a signal of interest from background, provides real-time monitoring of a chemical environment, and permits the use of several different fluorescent dyes h...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3368997
更新日期:2010-03-01 00:00:00
abstract::The recycling of G-protein-coupled receptors (GPCR) to the cell surface after internalization plays an important role in the regulation of overall GPCR activity. The angiotensin II type I receptor (AT1R) belongs to class B GPCRs that recycle slowly back to the cell surface. Previous studies have proposed that Rab11 co...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2943286
更新日期:2008-05-01 00:00:00
abstract::A new imaging approach, structured light scatteroscopy (SLS), is demonstrated, which offers rapid wide-field imaging of microscopic morphological variations in bulk tissue surfaces. Elastic scattering of light offers exquisite sensitivity to ultrastructural changes at multiple size scales ranging from nanometers to mi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.7.070504
更新日期:2014-01-01 00:00:00
abstract::To improve surgical guidance toward prostate draining lymph nodes, we investigate the potential of intraoperative fluorescence imaging and combined pre- and intraoperative multimodality imaging approaches. Transgenic adenocarcinoma mouse prostate mice with spontaneous prostate tumors are injected intratumorally with: ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3528027
更新日期:2011-01-01 00:00:00
abstract::As small animal optical imaging and tomography are gaining popularity for interrogating functional and molecular events in vivo, it becomes increasingly necessary to gain knowledge of the optical properties of the species investigated to better understand and describe photon propagation through their tissues. To achie...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2400702
更新日期:2006-11-01 00:00:00
abstract::We present three-dimensional (3-D) high-resolution spectral-domain optical coherence microscopy (SD-OCM) by using a supercontinuum (SC) fiber laser source with 300-nm spectral bandwidth (full-width at half-maximum) in the 1700-nm spectral band. By using low-coherence interferometry with SC light and a confocal detecti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.7.070502
更新日期:2019-07-01 00:00:00
abstract:SIGNIFICANCE:Photothermal optical coherence tomography (PT-OCT) has the promise to offer structural images coregistered with chemical composition information, which can offer a significant impact in early detection of diseases such as atherosclerosis. AIM:We take the first step in understanding the relation between PT...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.12.120501
更新日期:2020-12-01 00:00:00
abstract::Quantitative polarized light microscopy (qPLM) is a popular tool for the investigation of birefringent architectures in biological tissues. Collagen, the most abundant protein in mammals, is such a birefringent material. Interpretation of results of qPLM in terms of collagen network architecture and anisotropy is chal...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3241986
更新日期:2009-09-01 00:00:00
abstract::We describe the implementation and use of an adaptive optics loop in the imaging path of a commercial wide field microscope. We show that it is possible to maintain the optical performances of the original microscope when imaging through aberrant biological samples. The sources used for illuminating the adaptive optic...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3603847
更新日期:2011-07-01 00:00:00
abstract::Toward developing a new method for restoring tissue viscoelasticity in scarred vocal folds, we previously proposed a method to localize biomaterials within subepithelial voids ablated using ultrafast laser pulses. The clinical implementation of this method necessitates the quantification of the laser parameters for ab...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.11.118001
更新日期:2013-11-01 00:00:00
abstract::Pelvic organ prolapse (POP) occurs when changes to the pelvic organ support structures cause descent or herniation of the pelvic organs into the vagina. Clinical evaluation of POP is a series of manual measurements known as the pelvic organ prolapse quantification (POP-Q) score. However, it fails to identify the mecha...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.12.127008
更新日期:2016-12-01 00:00:00
abstract::Pedicle screw (PS) fixation has been widely used for spine diseases. Scientists and clinicians employ several approaches to navigate PS during operation. We have demonstrated the feasibility of monitoring the reduced scattering coefficient (μ's) on the trajectory of PS using near-infrared spectroscopy (NIRS). To perfo...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.11.117002
更新日期:2014-01-01 00:00:00
abstract::Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries res...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3251044
更新日期:2009-09-01 00:00:00
abstract::Intercellular heterogeneity is a key factor in a variety of core cellular processes including proliferation, stimulus response, carcinogenesis, and drug resistance. However, cell-to-cell variability studies at the single-cell level have been hampered by the lack of enabling experimental techniques. We present a measur...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.3.037008
更新日期:2012-03-01 00:00:00
abstract::Optically sectioned detection of fluorescence immunoassays using a confocal microscope enables the creation of both homo- and heterogeneous planar format assays. We report a set assays requiring optically sectioned detection using a model system and analysis procedures for separating signals of a surface layer from an...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3268772
更新日期:2009-11-01 00:00:00
abstract::We measure the tumor vascular response to varying irradiance rates during photodynamic therapy (PDT) in a Dunning rat prostate model with interstitial Doppler optical coherence tomography (IS-DOCT). Rats are given a photosensitizer drug, Photofrin, and the tumors are exposed to light (635 nm) with irradiance rates ran...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2744068
更新日期:2007-05-01 00:00:00
abstract::When irradiated with nanosecond laser pulses, gold nanoparticles allow for manipulation or destruction of cells and proteins with high spatial and temporal precision. Gold nanorods are especially attractive, because they have an up-to-20-fold stronger absorption than a sphere of equal volume, which is shifted to the o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.5.058003
更新日期:2012-05-01 00:00:00
abstract::In order to understand the relationship between accommodation and vision quality, a custom-built ultra-long scan depth spectral domain optical coherence tomography (UL-SDOCT) and a Shack-Hartmann wavefront sensor (HSWFS) were combined. The resolution and scan depth of UL-SDOCT are 6 μm and 15.6 mm, respectively, which...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.JBO.17.12.120501
更新日期:2012-12-01 00:00:00
abstract::Microstructures of unstained human melanoma skin tissues have been examined by multimodal nonlinear optical microscopy. The polarized shape of the individual melanoma cell can be readily recognized-a phenotype that has been identified in laboratory cultures as characteristic of proliferating melanocytes but has not be...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3120491
更新日期:2009-03-01 00:00:00
abstract::Type I collagen is a major component of the extracellular matrix as well as many tissue engineered models. To understand changes in collagen related models over time, it is important to evaluate collagen dynamics with noninvasive techniques. Fluorescence spectroscopy provides a method to noninvasively measure endogeno...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2360516
更新日期:2006-09-01 00:00:00
abstract::By applying linear perturbation theory to the radiation transport equation, the inverse problem of optical diffusion tomography can be reduced to a set of linear equations, Wμ=R, where W is the weight function, μ are the cross-section perturbations to be imaged, and R is the detector readings perturbations. We have st...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429870
更新日期:1998-04-01 00:00:00
abstract::We present experiments and analyses of confocal reflectance and two-photon microscopy studies of zebra finch skull samples. The thin and hollow structure of these birds' skulls is quite translucent, which can allow in vivo transcranial two-photon imaging for brain activation monitoring. However, the skull structure is...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3155522
更新日期:2009-05-01 00:00:00
abstract::We present a multimodal optical microscope that incorporates six imaging modalities on one common platform. The imaging modalities include three staring modes, optical quadrature microscopy (OQM), differential interference contrast (DIC) microscopy, and epi-fluorescence microscopy, and three scanning modes, confocal r...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2753744
更新日期:2007-07-01 00:00:00
abstract::Multiphoton microscopy (MPM) has the capacity to record second-harmonic generation (SHG) and endogenous two-photon excitation fluorescence (2PEF) signals emitted from biological tissues. The development of fiber-based miniaturized endomicroscopes delivering pulses in the femtosecond range will allow the transfer of MP...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.10.106004
更新日期:2019-10-01 00:00:00
abstract::Using the multiresolution ability of wavelets and effectiveness of singular value decomposition (SVD) to identify statistically robust parameters, we find a number of local and global features, capturing spectral correlations in the co- and cross-polarized channels, at different scales (of human breast tissues). The c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3606563
更新日期:2011-08-01 00:00:00