Abstract:
:Multiphoton microscopy (MPM) has the capacity to record second-harmonic generation (SHG) and endogenous two-photon excitation fluorescence (2PEF) signals emitted from biological tissues. The development of fiber-based miniaturized endomicroscopes delivering pulses in the femtosecond range will allow the transfer of MPM to clinical endoscopy. We present real-time SHG and 2PEF ex vivo images using an endomicroscope, which totally complies with clinical endoscopy regulations. This system is based on the proximal scanning of a commercial multicore image guide (IG). For understanding the inhomogeneities of the recorded images, we quantitatively characterize the IG at the single-core level during nonlinear excitation. The obtained results suggest that these inhomogeneities originate from the variable core geometries that, therefore, exhibit variable nonlinear and dispersive properties. Finally, we propose a method based on modulation of dispersion precompensation to address the image inhomogeneity issue and, as a proof of concept, we demonstrate its capability to improve the nonlinear image quality.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Garofalakis A,Kruglik SG,Mansuryan T,Gillibert A,Thiberville L,Louradour F,Vever-Bizet C,Bourg-Heckly Gdoi
10.1117/1.JBO.24.10.106004subject
Has Abstractpub_date
2019-10-01 00:00:00pages
1-12issue
10eissn
1083-3668issn
1560-2281pii
JBO-190020RRjournal_volume
24pub_type
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