Abstract:
:Optical resolution photoacoustic microscopy (ORPAM) is important for various biomedical applications, such as the study of cellular structures, microcirculation systems, and tumor angiogenesis. However, the lateral resolution of a conventional ORPAM is limited by optical diffraction. In this work, we report a simulation study to achieve subdiffraction-limited super-resolution in ORPAM using microspheres. Laser radiation is focused through a microsphere to generate a photonic nanojet, which provides the possibility to break the diffraction limit in ORPAM by reducing the size of the excitation volume. In our simulations using microspheres, we observed improvement in the lateral resolution up to compared to conventional ORPAM. The method is simple, cost effective, and can provide far-field resolution. This approach may provide new opportunities for many biomedical imaging applications that require finer resolution.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Upputuri PK,Wen ZB,Wu Z,Pramanik Mdoi
10.1117/1.JBO.19.11.116003subject
Has Abstractpub_date
2014-01-01 00:00:00pages
116003issue
11eissn
1083-3668issn
1560-2281pii
1926222journal_volume
19pub_type
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