Measuring the dynamics of cyclic adenosine monophosphate level in living cells induced by low-level laser irradiation using bioluminescence resonance energy transfer.

abstract：:Infrared microscopic imaging has been utilized to analyze for the first time the spatial distribution of lipid structure in an ex vivo human organ culture skin wound healing model. Infrared images were collected at zero, two, four, and six days following wounding. Analysis of lipid infrared spectral properties reveale...

journal_title：Journal of biomedical optics

authors： Yu G,Stojadinovic O,Tomic-Canic M,Flach CR,Mendelsohn R

更新日期：2012-09-01 00:00:00

abstract：:According to the valence asymmetry hypothesis, the left/right asymmetry of prefrontal cortex (PFC) activity is correlated with specific emotional responses to mental stress and personality traits. Here, we evaluated the relation between emotional state and asymmetry in PFC activity at rest by using near-infrared spect...

journal_title：Journal of biomedical optics

authors： Ishikawa W,Sato M,Fukuda Y,Matsumoto T,Takemura N,Sakatani K

更新日期：2014-02-01 00:00:00

abstract：:Fluorescence molecular tomography (FMT) is an important in vivo imaging modality to visualize physiological and pathological processes in small animals. However, FMT reconstruction is ill-posed and ill-conditioned due to strong optical scattering in deep tissues, which results in poor spatial resolution. It is well kn...

journal_title：Journal of biomedical optics

authors： Baikejiang R,Zhao Y,Fite BZ,Ferrara KW,Li C

更新日期：2017-05-01 00:00:00

abstract：:Pelvic organ prolapse (POP) occurs when changes to the pelvic organ support structures cause descent or herniation of the pelvic organs into the vagina. Clinical evaluation of POP is a series of manual measurements known as the pelvic organ prolapse quantification (POP-Q) score. However, it fails to identify the mecha...

journal_title：Journal of biomedical optics

authors： Parkinson LA,Gargett CE,Young N,Rosamilia A,Vashi AV,Werkmeister JA,Papageorgiou AW,Arkwright JW

更新日期：2016-12-01 00:00:00

abstract：:Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries res...

journal_title：Journal of biomedical optics

authors： Yao J,Maslov K,Hu S,Wang LV

更新日期：2009-09-01 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopic imaging has been used to probe the biochemical composition of human renal tumor tissue and adjacent normal tissue. Freshly resected renal tumor tissue from surgery was prepared as a thin cryosection and examined by FTIR spectroscopic imaging. Tissue types could be discri...

journal_title：Journal of biomedical optics

authors： Sablinskas V,Urboniene V,Ceponkus J,Laurinavicius A,Dasevicius D,Jankevicius F,Hendrixson V,Koch E,Steiner G

更新日期：2011-09-01 00:00:00

abstract：:We describe a computer eye model that allows for aspheric surfaces and a three-dimensional computer-based ray-tracing technique to simulate optical properties of the human eye and visual perception under various eye defects. Eye surfaces, such as the cornea, eye lens, and retina, are modeled or approximated by a set o...

journal_title：Journal of biomedical optics

authors： Fink W,Micol D

更新日期：2006-09-01 00:00:00

abstract：:Knowledge of neuronal wiring and morphogenesis in Drosophila is essential to understand brain function and dysfunction. The immunoenzyme method based on horseradish peroxidase/diaminobenzidine (HRP/DAB) provides high-contrast images to resolve details underlying neuronal architecture. However, the poor staining penetr...

journal_title：Journal of biomedical optics

authors： Tian Q,Yuan J,Li Y,Jiang T,Gong H,Zhou W

更新日期：2014-09-01 00:00:00

abstract：:A multifunctional line scanning ophthalmoscope (mLSO) was designed, constructed, and tested on human subjects. The mLSO could sequentially acquire wide-field, confocal, near-infrared reflectance, fluorescein angiography (FA), and indocyanine green angiography (ICGA) retinal images. The system also included a retinal t...

journal_title：Journal of biomedical optics

authors： Hammer DX,Ferguson RD,Patel AH,Vazquez V,Husain D

更新日期：2012-02-01 00:00:00

abstract：:Reduction of optical scattering in turbid biological tissues using nonreactive chemical agents has potential applications for light-based diagnostics and therapeutics. Optical clearing effects by exogenous chemical agents, in particular sugars and sugar alcohols, have been found to be temporary with tissue rehydration...

journal_title：Journal of biomedical optics

authors： Yeh AT,Hirshburg J

更新日期：2006-01-01 00:00:00

abstract：:The application of near-infrared spectroscopy (NIRS) to assess microvascular function has shown promising results. An important limitation when using a single source-detector pair, however, is the lack of depth sensitivity. Diffuse optical tomography (DOT) overcomes this limitation using an array of sources and detect...

journal_title：Journal of biomedical optics

authors： Vidal-Rosas EE,Billings SA,Chico T,Coca D

更新日期：2016-06-01 00:00:00

abstract：:Nonviral, site-specific gene delivery to deep tissue is required for gene therapy of a spinal cord injury. However, an efficient method satisfying these requirements has not been established. This study demonstrates efficient and targeted gene transfer into the spinal cord by using photomechanical waves (PMWs), which ...

journal_title：Journal of biomedical optics

authors： Ando T,Sato S,Toyooka T,Uozumi Y,Nawashiro H,Ashida H,Obara M

更新日期：2011-10-01 00:00:00

abstract：:We theoretically investigate the effect of noise on frequency-domain heterodyne and/or homodyne measurements of intensity-modulated beams propagating through diffusive media, such as a photon density wave. We assumed that the attenuated amplitude and delayed phase are estimated by taking the Fourier transform of the n...

journal_title：Journal of biomedical optics

authors： Kang D,Kupinski MA

更新日期：2012-01-01 00:00:00

abstract：:As the most abundant cell type in the central nervous system, astrocyte has been one of main research topics in neuroscience. Although various tools have been developed, at present, there is no tool that allows noninvasive activation of astrocyte in vivo without genetic or pharmacological perturbation. Here we report ...

journal_title：Journal of biomedical optics

authors： Choi M,Yoon J,Ku T,Choi K,Choi C

更新日期：2011-07-01 00:00:00

abstract：:We address the automatic differentiation of human tissue using multispectral imaging with promising potential for automatic visualization during surgery. Currently, tissue types have to be continuously differentiated based on the surgeon's knowledge only. Further, automatic methods based on optical in vivo properties ...

journal_title：Journal of biomedical optics

authors： Wisotzky EL,Uecker FC,Arens P,Dommerich S,Hilsmann A,Eisert P

更新日期：2018-05-01 00:00:00

abstract：:Although perfectly transparent specimens are imaged faster with light-sheet microscopy, less transparent samples are often imaged with two-photon microscopy leveraging its robustness to scattering; however, at the price of increased acquisition times. Clearing methods that are capable of rendering strongly scattering ...

journal_title：Journal of biomedical optics

authors： Adam MP,Müllenbroich MC,Di Giovanna AP,Alfieri D,Silvestri L,Sacconi L,Pavone FS

更新日期：2018-02-01 00:00:00

abstract：:Current techniques to assess lymph node metastases in cancer patients include lymphoscintigraphy after administration of a nonspecific radiocolloid in order to locate and resect lymph nodes for pathological examination of harbored cancer cells. Clinical trials involving intradermal or subcutaneous injection of antibod...

journal_title：Journal of biomedical optics

authors： Sampath L,Wang W,Sevick-Muraca EM

更新日期：2008-07-01 00:00:00

abstract：:Cellularity of the fibrous caps of coronary atheromas, manifested by the infiltration of macrophages (average size, 20 to 30 microm), is thought to weaken the structural integrity of the cap and predispose plaques to rupture. Therefore, an imaging technology capable of identifying macrophages within fibroatheroma caps...

journal_title：Journal of biomedical optics

authors： Tanaka A,Tearney GJ,Bouma BE

更新日期：2010-01-01 00:00:00

abstract：:Three major analytical tools in imaging science are summarized and demonstrated relative to optical imaging in vivo. Standard resolution testing is optimal when infinite contrast is used and hardware evaluation is the goal. However, deep tissue imaging of absorption or fluorescent contrast agents in vivo often present...

journal_title：Journal of biomedical optics

authors： Pogue BW,Davis SC,Song X,Brooksby BA,Dehghani H,Paulsen KD

更新日期：2006-05-01 00:00:00

abstract：:Cochlear implantation offers the potential to restore sensitive hearing in patients with severe to profound deafness. However, surgical placement of the electrode array within the cochlea can produce trauma to sensorineural components, particularly if the initial turn of the cochlea is not successfully navigated as th...

journal_title：Journal of biomedical optics

authors： Gurbani SS,Wilkening P,Zhao M,Gonenc B,Cheon GW,Iordachita II,Chien W,Taylor RH,Niparko JK,Kang JU

更新日期：2014-05-01 00:00:00

abstract：:Deep anterior lamellar keratoplasty (DALK) is an emerging surgical technique for the restoration of corneal clarity and vision acuity. The big-bubble technique in DALK surgery is the most essential procedure that includes the air injection through a thin syringe needle to separate the dysfunctional region of the corne...

journal_title：Journal of biomedical optics

authors： Shin S,Bae JK,Ahn Y,Kim H,Choi G,Yoo YS,Joo CK,Moon S,Jung W

更新日期：2017-12-01 00:00:00

abstract：:The effect of sampling region size and tissue heterogeneity is examined using fluorescence histogram assessment in a rat prostate tumor model with benzoporphyrin derivative fluorophore. Spatial heterogeneity in the fluorescence signal occurs on both macroscopic and microscopic scales. The periphery of the tumor is mor...

journal_title：Journal of biomedical optics

authors： Pogue BW,Chen B,Zhou X,Hoopes PJ

更新日期：2005-07-01 00:00:00

abstract：:Optical technologies may be applied to multiple facets of spinal surgery from diagnostics to intraoperative image guidance to therapeutics. In diagnostics, the current standard remains cross-sectional static imaging. Optical surface scanning tools may have an important role; however, significant work is required to cl...

journal_title：Journal of biomedical optics

authors： Guha D,Yang VXD

更新日期：2018-06-01 00:00:00

abstract：:Protoporphyrin IX (PpIX)-encapsulated mesoporous silica nanoparticles were synthesized, characterized, and utilized for photodynamic therapy (PDT) of cancer. Silica encapsulation is relatively transparent for activated light and can protect the PpIX against denaturation induced by the extreme bioenvironment. The mesop...

journal_title：Journal of biomedical optics

authors： Qian J,Gharibi A,He S

更新日期：2009-01-01 00:00:00

abstract：:We demonstrate a single-camera imaging system that can simultaneously acquire brightfield, darkfield, and phase contrast images in real time. Our method uses computational illumination via a programmable light-emitting diode (LED) array at the source plane, providing flexible patterning of illumination angles. Brightf...

journal_title：Journal of biomedical optics

authors： Liu Z,Tian L,Liu S,Waller L

更新日期：2014-01-01 00:00:00

abstract：:The lymphatic system is responsible for fluid homeostasis and immune cell trafficking and has been implicated in several diseases, including obesity, diabetes, and cancer metastasis. Despite its importance, the lack of suitable in vivo imaging techniques has hampered our understanding of the lymphatic system. This is,...

journal_title：Journal of biomedical optics

authors： Forbrich A,Heinmiller A,Zemp RJ

更新日期：2017-10-01 00:00:00

abstract：:We fabricate scaffolds with a fine linear grating (periodicity of 1 to 8 microm and height of 1 microm) on a glass substrate for controlling cardiomyocyte orientation. The fabrication is done by the solidification of a liquid photopolymerizable material using two laser beam interference. As the photopolymerizable mate...

journal_title：Journal of biomedical optics

authors： Fujita A,Fujita K,Nakamura O,Matsuda T,Kawata S

更新日期：2006-03-01 00:00:00

abstract：:A simultaneous multispectral fluorescence imaging system incorporating multiplexed volume holographic grating (VHG) is developed to acquire multispectral images of an object in one shot. With the multiplexed VHG, the imaging system can provide the distribution and spectral characteristics of multiple fluorophores in t...

journal_title：Journal of biomedical optics

authors： Lv Y,Zhang J,Zhang D,Cai W,Chen N,Luo J

更新日期：2016-06-01 00:00:00

abstract：:Surgical light is important for helping the surgeon easily identify specific tissues during an operation. We propose a spectral reflectance comparison model to optimize the light-emitting diode light spectrum in the operating room. An entropy evaluation method, meant specifically for surgical situations, was developed...

journal_title：Journal of biomedical optics

authors： Shen J,Wang H,Wu Y,Li A,Chen C,Zheng Z

更新日期：2015-10-01 00:00:00

abstract：:Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak...

journal_title：Journal of biomedical optics

authors： Krishnan RV,Masuda A,Centonze VE,Herman B

更新日期：2003-07-01 00:00:00