Abstract:
:We demonstrate a single-camera imaging system that can simultaneously acquire brightfield, darkfield, and phase contrast images in real time. Our method uses computational illumination via a programmable light-emitting diode (LED) array at the source plane, providing flexible patterning of illumination angles. Brightfield, darkfield, and differential phase contrast images are obtained by changing the LED patterns, without any moving parts. Previous work with LED array illumination was only valid for static samples because the hardware speed was not fast enough to meet real-time acquisition and processing requirements. Here, we time multiplex patterns for each of the three contrast modes in order to image dynamic biological processes in all three contrast modes simultaneously. We demonstrate multicontrast operation at the maximum frame rate of our camera (50 Hz with 2160 × 2560 pixels).
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Liu Z,Tian L,Liu S,Waller Ldoi
10.1117/1.JBO.19.10.106002subject
Has Abstractpub_date
2014-01-01 00:00:00pages
106002issue
10eissn
1083-3668issn
1560-2281pii
1913607journal_volume
19pub_type
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