Abstract:
:Infrared microscopic imaging has been utilized to analyze for the first time the spatial distribution of lipid structure in an ex vivo human organ culture skin wound healing model. Infrared images were collected at zero, two, four, and six days following wounding. Analysis of lipid infrared spectral properties revealed the presence of a lipid class with disordered chains within and in the vicinity of the migrating epithelial tongue. The presence of lipid ester C=O bands colocalized with the disordered chains provided evidence for the presence of carbonyl-containing lipid species. Gene array data complemented the biophysical studies and provided a biological rationale for the generation of the disordered chain species. This is the first clear observation, to our knowledge, of disordered lipid involvement in cutaneous wound healing. Several possibilities are discussed for the biological relevance of these observations.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Yu G,Stojadinovic O,Tomic-Canic M,Flach CR,Mendelsohn Rdoi
10.1117/1.JBO.17.9.096009subject
Has Abstractpub_date
2012-09-01 00:00:00pages
96009-1issue
9eissn
1083-3668issn
1560-2281pii
1357032journal_volume
17pub_type
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