Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850 nm.

abstract：:We experimentally implement label-free phase microscopy using wide-field digital interferometry (WFDI) techniques to retrieve quantitative volumetric data of articular chondrocyte dynamics. Using the scanless interferometric system, we visualize chondrocyte swelling and bursting induced by hypo-osmotic pressure. Recon...

journal_title：Journal of biomedical optics

authors： Shaked NT,Finan JD,Guilak F,Wax A

更新日期：2010-01-01 00:00:00

abstract：:The damage mechanism for near-infrared radiation (IRR) induced cataract is unclear. Both a photochemical and a thermal mechanism were suggested. The current paper aims to elucidate a photochemical effect based on investigation of irradiance-exposure time reciprocity. Groups of 20 rats were unilaterally exposed to 96-W...

journal_title：Journal of biomedical optics

authors： Yu Z,Schulmeister K,Talebizadeh N,Kronschläger M,Söderberg P

更新日期：2015-01-01 00:00:00

abstract：:Studies of bioluminescence in living animals, such as cell-based biosensor applications, require measurement of light at different wavelengths, but accurate light measurement is impeded by absorption by tissues at wavelengths<600 nm. We present a novel approach to this problem--the use of a plastic window in the skin/...

journal_title：Journal of biomedical optics

authors： Huang Q,Acha V,Yow R,Schneider E,Sardar DK,Hornsby PJ

更新日期：2007-09-01 00:00:00

abstract：:We present a system combining optical coherence tomography (OCT) and multispectral imaging (MSI) for coregistered structural imaging and surface color imaging. We first describe and numerically validate an optimization model to guide the selection of the MSI wavelengths and their relative intensities. We then demonstr...

journal_title：Journal of biomedical optics

authors： Attendu X,Bourget MH,de Sivry-Houle MP,Boudoux C

更新日期：2019-11-01 00:00:00

abstract：:Field carcinogenesis is the initial stage of cancer progression. Understanding field carcinogenesis is valuable for both cancer biology and clinical medicine. Here, we used inverse spectroscopic optical coherence tomography to study colorectal cancer (CRC) and pancreatic cancer (PC) field carcinogenesis. Depth-resolve...

journal_title：Journal of biomedical optics

authors： Yi J,Radosevich AJ,Stypula-Cyrus Y,Mutyal NN,Azarin SM,Horcher E,Goldberg MJ,Bianchi LK,Bajaj S,Roy HK,Backman V

更新日期：2014-03-01 00:00:00

abstract：:Treatment of deep venous thrombosis (DVT)--a primary cause of potentially fatal pulmonary embolism (PE)--depends on the age of the thrombus. The existing clinical imaging methods are capable of visualizing a thrombus but cannot determine the age of the blood clot. Therefore, there is a need for an imaging technique to...

journal_title：Journal of biomedical optics

authors： Karpiouk AB,Aglyamov SR,Mallidi S,Shah J,Scott WG,Rubin JM,Emelianov SY

更新日期：2008-09-01 00:00:00

abstract：:Skin cancer diagnosis depends not only on histopathological examination but also on visual inspection before and after the excision of suspected lesion. Neoplasm is accompanied with changes in birefringence of collagen, pleomorphicity, and hyperchromatic state of epithelial nuclei. These phenomena can be measured by s...

journal_title：Journal of biomedical optics

authors： Aharon O,Abdulhalim I,Arnon O,Rosenberg L,Dyomin V,Silberstein E

更新日期：2011-08-01 00:00:00

abstract：:The development of methods that allow microscale studies of complex biomaterials based on their molecular composition is of great interest to a wide range of research fields. We show that stimulated Raman scattering (SRS) microscopy is an excellent analytical tool to study distributions of different biomolecules in mu...

journal_title：Journal of biomedical optics

authors： Roeffaers MB,Zhang X,Freudiger CW,Saar BG,van Ruijven M,van Dalen G,Xiao C,Xie XS

更新日期：2010-11-01 00:00:00

abstract：:A swept source (SS)-based circular-state (CS) polarization-sensitive optical coherence tomography (PS-OCT) constructed entirely with polarization-maintaining fiber optics components is proposed with the experimental verification. By means of the proposed calibration scheme, bulk quarter-wave plates can be replaced by ...

journal_title：Journal of biomedical optics

authors： Lin H,Kao MC,Lai CM,Huang JC,Kuo WC

更新日期：2014-02-01 00:00:00

abstract：:Diffusion of two model drugs-benzyl nicotinate and ibuprofen-and the plasma macromolecule albumin across atherosclerotic rabbit aorta was studied ex vivo by attenuated total reflection-Fourier transform infrared (ATR-FTIR) imaging. Solutions of these molecules were applied to the endothelial surface of histological se...

journal_title：Journal of biomedical optics

authors： Palombo F,Danoux CB,Weinberg PD,Kazarian SG

更新日期：2009-07-01 00:00:00

abstract：:Amblyopia ("lazy eye") is a major public health problem, caused by misalignment of the eyes (strabismus) or defocus. If detected early in childhood, there is an excellent response to therapy, yet most children are detected too late to be treated effectively. Commercially available vision screening devices that test fo...

journal_title：Journal of biomedical optics

authors： Irsch K,Gramatikov BI,Wu YK,Guyton DL

更新日期：2014-06-01 00:00:00

abstract：:We present a simple wide-field imaging technique, called HiLo microscopy, that is capable of producing optically sectioned images in real time, comparable in quality to confocal laser scanning microscopy. The technique is based on the fusion of two raw images, one acquired with speckle illumination and another with st...

journal_title：Journal of biomedical optics

authors： Lim D,Ford TN,Chu KK,Mertz J

更新日期：2011-01-01 00:00:00

abstract：SIGNIFICANCE:Our study introduces an application of deep learning to virtually generate fluorescence images to reduce the burdens of cost and time from considerable effort in sample preparation related to chemical fixation and staining. AIM:The objective of our work was to determine how successfully deep learning meth...

journal_title：Journal of biomedical optics

authors： Nguyen T,Bui V,Thai A,Lam V,Raub C,Chang LC,Nehmetallah G

更新日期：2020-09-01 00:00:00

abstract：:Intercellular heterogeneity is a key factor in a variety of core cellular processes including proliferation, stimulus response, carcinogenesis, and drug resistance. However, cell-to-cell variability studies at the single-cell level have been hampered by the lack of enabling experimental techniques. We present a measur...

journal_title：Journal of biomedical optics

authors： Kelbauskas L,Ashili SP,Houkal J,Smith D,Mohammadreza A,Lee KB,Forrester J,Kumar A,Anis YH,Paulson TG,Youngbull CA,Tian Y,Holl MR,Johnson RH,Meldrum DR

更新日期：2012-03-01 00:00:00

abstract：:The recycling of G-protein-coupled receptors (GPCR) to the cell surface after internalization plays an important role in the regulation of overall GPCR activity. The angiotensin II type I receptor (AT1R) belongs to class B GPCRs that recycle slowly back to the cell surface. Previous studies have proposed that Rab11 co...

journal_title：Journal of biomedical optics

authors： Li H,Li HF,Felder RA,Periasamy A,Jose PA

更新日期：2008-05-01 00:00:00

abstract：:UV-induced collagen cross-linking is a promising treatment for keratoconus that stiffens corneal tissue and prevents further degeneration. Since keratoconus is generally localized, the efficacy of collagen cross-linking (CXL) treatments could be improved by stiffening only the weakened parts of the cornea. Here, we de...

journal_title：Journal of biomedical optics

authors： Singh M,Li J,Vantipalli S,Han Z,Larin KV,Twa MD

更新日期：2017-09-01 00:00:00

abstract：:We report the design, integration, and validation of a fluorescence microscopy system for imaging of electroperturbation--the effects of nanosecond, megavolt-per-meter pulsed electric fields on biological cells and tissues. Such effects have potential applications in cancer therapy, gene regulation, and biophysical re...

journal_title：Journal of biomedical optics

authors： Sun Y,Vernier PT,Behrend M,Wang J,Thu MM,Gundersen M,Marcu L

更新日期：2006-03-01 00:00:00

abstract：:We present a study using plasmonic nanoparticles (NPs) to image epidermal growth factor receptor (EGFR) in live cells. Through detailed analysis of the NP scattering spectra, we determine the intracellular refractive index (RI) within attoliter volumes inside of the living cells. Molecular imaging is demonstrated usin...

journal_title：Journal of biomedical optics

authors： Curry AC,Crow M,Wax A

更新日期：2008-01-01 00:00:00

abstract：:We describe the implementation and use of an adaptive optics loop in the imaging path of a commercial wide field microscope. We show that it is possible to maintain the optical performances of the original microscope when imaging through aberrant biological samples. The sources used for illuminating the adaptive optic...

journal_title：Journal of biomedical optics

authors： Vermeulen P,Muro E,Pons T,Loriette V,Fragola A

更新日期：2011-07-01 00:00:00

abstract：:Nonmelanoma skin cancer is the most common form of human cancer, often resulting in high morbidity. Low visual contrast of these tumors makes their delineation a challenging problem. Employing a linearly polarized monochromatic light source and a wide-field CCD camera, we have developed a technique for fluorescence po...

journal_title：Journal of biomedical optics

authors： Yaroslavsky AN,Salomatina EV,Neel V,Anderson R,Flotte T

更新日期：2007-01-01 00:00:00

abstract：:A reflectance volume holographic imaging (VHI) endoscope has been designed for simultaneous in vivo imaging of surface and subsurface tissue structures. Prior utilization of VHI systems has been limited to ex vivo tissue imaging. The VHI system presented in this work is designed for laparoscopic use. It consists of a ...

journal_title：Journal of biomedical optics

authors： Howlett ID,Han W,Gordon M,Rice P,Barton JK,Kostuk RK

更新日期：2017-05-01 00:00:00

abstract：:Parametric and nonparametric data processing schemes for analyzing translating laser speckle data used to investigate the mechanical behavior of biological tissues are examined. Cross-correlation, minimum mean square estimator, maximum likelihood, and maximum entropy approaches are discussed and compared on speckle da...

journal_title：Journal of biomedical optics

authors： Duncan DD,Kirkpatrick SJ

更新日期：2001-10-01 00:00:00

abstract：:A simple empirical method is presented to determine the scattering coefficient mu' s from backscattered polarized images of turbid media. It uses the ratio, pixel by pixel, of two images that are the second and the first backscattered Stokes parameter images Q and I, respectively. Taking this image ratio, then integra...

journal_title：Journal of biomedical optics

authors： Jaillon F,Saint-Jalmes H

更新日期：2005-05-01 00:00:00

abstract：:Peripheral nerve injury in vivo promotes a regenerative growth in vitro characterized by an improved neurite regrowth. Knowledge of the conditioning injury effects on both morphology and mechanical properties of live sensory neurons could be instrumental to understand the cellular and molecular mechanisms leading to t...

journal_title：Journal of biomedical optics

authors： Benzina O,Szabo V,Lucas O,Saab MB,Cloitre T,Scamps F,Gergely C,Martin M

更新日期：2013-10-01 00:00:00

abstract：:Waveguide evanescent field fluorescence (WEFF) microscopy is a new development that allows the imaging of contact regions between biological cells and their substratum, as well as imaging of ultrathin films such as monomolecular Langmuir-Blodgett (LB) films. Mixed-lipid monolayer systems mimicking lung surfactant were...

journal_title：Journal of biomedical optics

authors： Hassanzadeh A,Mittler S

更新日期：2011-04-01 00:00:00

abstract：:Second-harmonic generation (SHG) imaging can help reveal interactions between collagen fibers and cancer cells. Quantitative analysis of SHG images of collagen fibers is challenged by the heterogeneity of collagen structures and low signal-to-noise ratio often found while imaging collagen in tissue. The role of collag...

journal_title：Journal of biomedical optics

authors： Bredfeldt JS,Liu Y,Pehlke CA,Conklin MW,Szulczewski JM,Inman DR,Keely PJ,Nowak RD,Mackie TR,Eliceiri KW

更新日期：2014-01-01 00:00:00

abstract：:Although perfectly transparent specimens are imaged faster with light-sheet microscopy, less transparent samples are often imaged with two-photon microscopy leveraging its robustness to scattering; however, at the price of increased acquisition times. Clearing methods that are capable of rendering strongly scattering ...

journal_title：Journal of biomedical optics

authors： Adam MP,Müllenbroich MC,Di Giovanna AP,Alfieri D,Silvestri L,Sacconi L,Pavone FS

更新日期：2018-02-01 00:00:00

abstract：:All aberrations produced inside a biospecimen can degrade the quality of a three-dimensional image in two-photon excitation laser scanning microscopy. Previously, we developed a transmissive liquid-crystal device to correct spherical aberrations that improved the image quality of a fixed-mouse-brain slice treated with...

journal_title：Journal of biomedical optics

authors： Tanabe A,Hibi T,Ipponjima S,Matsumoto K,Yokoyama M,Kurihara M,Hashimoto N,Nemoto T

更新日期：2016-12-01 00:00:00

abstract：:We propose an algorithm to compensate for the refractive index error in the optical coherence tomography (OCT) images of multilayer tissues, such as skin. The performance of the proposed method has been evaluated on one- and two-layer solid phantoms, as well as the skin of rat paw. ...

journal_title：Journal of biomedical optics

authors： Turani Z,Fatemizadeh E,Xu Q,Daveluy S,Mehregan D,Nasiri Avanaki MR

更新日期：2018-07-01 00:00:00

abstract：:Time-deconvolution of the instrument response from fluorescence lifetime imaging microscopy (FLIM) data is usually necessary for accurate fluorescence lifetime estimation. In many applications, however, the instrument response is not available. In such cases, a blind deconvolution approach is required. An iterative me...

journal_title：Journal of biomedical optics

authors： Campos-Delgado DU,Gutierrez-Navarro O,Arce-Santana ER,Skala MC,Walsh AJ,Jo JA

更新日期：2015-07-01 00:00:00