Abstract:
:We experimentally implement label-free phase microscopy using wide-field digital interferometry (WFDI) techniques to retrieve quantitative volumetric data of articular chondrocyte dynamics. Using the scanless interferometric system, we visualize chondrocyte swelling and bursting induced by hypo-osmotic pressure. Reconstructed images are obtained by an efficient digital process. We use the resulting images to calculate quantitative temporal-spatial morphological parameters of the cell, with the observed dynamics limited only by the true frame rate of the camera. To show the utility of WFDI in recording articular chondrocyte dynamics, we also provide an experimental comparison of WFDI and differential interference contrast microscopy.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Shaked NT,Finan JD,Guilak F,Wax Adoi
10.1117/1.3290242subject
Has Abstractpub_date
2010-01-01 00:00:00pages
010505issue
1eissn
1083-3668issn
1560-2281journal_volume
15pub_type
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