FRET-based method for evaluation of the efficiency of reversible and irreversible sonoporation.


:It is widely known that not all of the treated cells survive after introduction of exogenous molecules via any physical method. Therefore, it is important to develop methods that would allow simultaneous evaluation of both molecular delivery efficiency and cell viability. This study presents Förster resonance energy transfer (FRET)-based method that allows molecular transfer and cell viability evaluation in a single measurement by employing two common fluorescent dyes, namely, ethidium bromide and trypan blue. The method has been validated using cell sonoporation. The FRET-based method allows the efficiency evaluation of both reversible and irreversible sonoporation in a single experiment. Therefore, this method could be used to reduce time, labor, and material cost while improving the accuracy of evaluations.


J Biomed Opt


Ruzgys P,Tamošiūnas M,Lukinsone V,Šatkauskas S




Has Abstract


2017-09-01 00:00:00














  • Detection of canine skin and subcutaneous tumors by visible and near-infrared diffuse reflectance spectroscopy.

    abstract::Cancer is the main cause of canine morbidity and mortality. The existing evaluation of tumors requires an experienced veterinarian and usually includes invasive procedures (e.g., fine-needle aspiration) that can be unpleasant for the dog and the owner. We investigate visible and near-infrared diffuse reflectance spect...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Cugmas B,Plavec T,Bregar M,Naglič P,Pernuš F,Likar B,Bürmen M

    更新日期:2015-03-01 00:00:00

  • Near-ultraviolet removal rates for subgingival dental calculus at different irradiation angles.

    abstract::The laser ablation rate of subgingival dental calculus irradiated at a 400-nm-wavelength, 7.4-mJ pulse energy, and 85- and 20-deg irradiation angles is measured using laser triangulation. Three-dimensional images taken before and after irradiation create a removal map with 6-μm axial resolution. Fifteen human teeth wi...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Schoenly JE,Seka WD,Rechmann P

    更新日期:2011-07-01 00:00:00

  • Influence of phase function on modeled optical response of nanoparticle-labeled epithelial tissues.

    abstract::Metal nanoparticles can be functionalized with biomolecules to selectively localize in precancerous tissues and can act as optical contrast enhancers for reflectance-based diagnosis of epithelial precancer. We carry out Monte Carlo (MC) simulations to analyze photon propagation through nanoparticle-labeled tissues and...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Cihan C,Arifler D

    更新日期:2011-08-01 00:00:00

  • Noninvasive optoacoustic temperature determination at the fundus of the eye during laser irradiation.

    abstract::In all fundus laser treatments of the eye, the temperature increase is not exactly known. In order to optimize treatments, an online temperature determination is preferable. We investigated a noninvasive optoacoustic method to monitor the fundus temperature during pulsed laser irradiation. When laser pulses are applie...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Schule G,Huttmann G,Framme C,Roider J,Brinkmann R

    更新日期:2004-01-01 00:00:00

  • Digital phantoms generated by spectral and spatial light modulators.

    abstract::A hyperspectral image projector (HIP) based on liquid crystal on silicon spatial light modulators is explained and demonstrated to generate data cubes. The HIP-constructed data cubes are three-dimensional images of the spatial distribution of spectrally resolved abundances of intracellular light-absorbing oxyhemoglobi...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章,收录出版


    authors: Chon B,Tokumasu F,Lee JY,Allen DW,Rice JP,Hwang J

    更新日期:2015-01-01 00:00:00

  • Time-resolved imaging of optical coefficients through murine chest cavities.

    abstract::As small animal optical imaging and tomography are gaining popularity for interrogating functional and molecular events in vivo, it becomes increasingly necessary to gain knowledge of the optical properties of the species investigated to better understand and describe photon propagation through their tissues. To achie...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Niedre MJ,Turner GM,Ntziachristos V

    更新日期:2006-11-01 00:00:00

  • Test-retest reliability of the prefrontal response to affective pictures based on functional near-infrared spectroscopy.

    abstract::Functional near-infrared spectroscopy (fNIRS) is being increasingly applied to affective and social neuroscience research; however, the reliability of this method is still unclear. This study aimed to evaluate the test–retest reliability of the fNIRS-based prefrontal response to emotional stimuli. Twenty-six participa...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Huang Y,Mao M,Zhang Z,Zhou H,Zhao Y,Duan L,Kreplin U,Xiao X,Zhu C

    更新日期:2017-01-01 00:00:00

  • Study of the inhibition effect of thiazone on muscle optical clearing.

    abstract::We investigated the effect of thiazone, a widely used penetration enhancer, on in vitro porcine skin and muscle tissue by single-integrating sphere technique during optical clearing (OC) treatment. The results showed that thiazone induced an increase on the total transmittance of skin which led to a reduction in that ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Jin X,Deng Z,Wang J,Ye Q,Mei J,Zhou W,Zhang C,Tian J

    更新日期:2016-10-01 00:00:00

  • Re-evaluation of model-based light-scattering spectroscopy for tissue spectroscopy.

    abstract::Model-based light scattering spectroscopy (LSS) seemed a promising technique for in-vivo diagnosis of dysplasia in multiple organs. In the studies, the residual spectrum, the difference between the observed and modeled diffuse reflectance spectra, was attributed to single elastic light scattering from epithelial nucle...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Lau C,Sćepanović O,Mirkovic J,McGee S,Yu CC,Fulghum S,Wallace M,Tunnell J,Bechtel K,Feld M

    更新日期:2009-03-01 00:00:00

  • Multimodal optical microscope for detecting viability of mouse embryos in vitro.

    abstract::We present a multimodal optical microscope that incorporates six imaging modalities on one common platform. The imaging modalities include three staring modes, optical quadrature microscopy (OQM), differential interference contrast (DIC) microscopy, and epi-fluorescence microscopy, and three scanning modes, confocal r...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Warger WC 2nd,Laevsky GS,Townsend DJ,Rajadhyaksha M,DiMarzio CA

    更新日期:2007-07-01 00:00:00

  • Quantum cascade laser-based hyperspectral imaging of biological tissue.

    abstract::The spectroscopy of analyte-specific molecular vibrations in tissue thin sections has opened up a path toward histopathology without the need for tissue staining. However, biomedical vibrational imaging has not yet advanced from academic research to routine histopathology due to long acquisition times for the microsco...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Kröger N,Egl A,Engel M,Gretz N,Haase K,Herpich I,Kränzlin B,Neudecker S,Pucci A,Schönhals A,Vogt J,Petrich W

    更新日期:2014-01-01 00:00:00

  • Imaging of skin birefringence for human scar assessment using polarization-sensitive optical coherence tomography aided by vascular masking.

    abstract::We demonstrate the in vivo assessment of human scars by parametric imaging of birefringence using polarization-sensitive optical coherence tomography (PS-OCT). Such in vivo assessment is subject to artifacts in the detected birefringence caused by scattering from blood vessels. To reduce these artifacts, we preprocess...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Gong P,Chin L,Es'haghian S,Liew YM,Wood FM,Sampson DD,McLaughlin RA

    更新日期:2014-12-01 00:00:00

  • Rise of Raman spectroscopy in neurosurgery: a review.

    abstract:SIGNIFICANCE:Although the clinical potential for Raman spectroscopy (RS) has been anticipated for decades, it has only recently been used in neurosurgery. Still, few devices have succeeded in making their way into the operating room. With recent technological advancements, however, vibrational sensing is poised to be a...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: DePaoli D,Lemoine É,Ember K,Parent M,Prud'homme M,Cantin L,Petrecca K,Leblond F,Côté DC

    更新日期:2020-05-01 00:00:00

  • Determination of penetration profiles of topically applied substances by means of tape stripping and optical spectroscopy: UV filter substance in sunscreens.

    abstract::Penetration profiles of topically applied drugs and cosmetic products provide important information on their efficacy. The application of tape stripping in combination with UV/VIS spectroscopy is checked to determine the local position of topically applied substances inside the stratum corneum, the penetration profile...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Weigmann HJ,Jacobi U,Antoniou C,Tsikrikas GN,Wendel V,Rapp C,Gers-Barlag H,Sterry W,Lademann J

    更新日期:2005-01-01 00:00:00

  • Quantitative tool for rapid disease mapping using optical coherence tomography images of azoxymethane-treated mouse colon.

    abstract::Optical coherence tomography (OCT) can provide new insight into disease progression and therapy by enabling nondestructive, serial imaging of in vivo cancer models. In previous studies, we have shown the utility of endoscopic OCT for identifying adenomas in the azoxymethane-treated mouse model of colorectal cancer and...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Winkler AM,Rice PF,Drezek RA,Barton JK

    更新日期:2010-07-01 00:00:00

  • Dual/differential coherent anti-Stokes Raman scattering module for multiphoton microscopes with a femtosecond Ti:sapphire oscillator.

    abstract::In the last decade, coherent anti-Stokes Raman scattering (CARS) microscopy has emerged as a powerful multiphoton imaging technique offering label-free chemical sensitivity and high three-dimensional resolution. However, its widespread application in the life sciences has been hampered by the use of costly pulsed lase...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Li B,Borri P,Langbein W

    更新日期:2013-06-01 00:00:00

  • Bipolar cellular morphology of malignant melanoma in unstained human melanoma skin tissue.

    abstract::Microstructures of unstained human melanoma skin tissues have been examined by multimodal nonlinear optical microscopy. The polarized shape of the individual melanoma cell can be readily recognized-a phenotype that has been identified in laboratory cultures as characteristic of proliferating melanocytes but has not be...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Zhang K,Zhang W,Yang CY,Yang H

    更新日期:2009-03-01 00:00:00

  • Parallel two-channel near- and far-field fluorescence microscopy.

    abstract::We report a new two-channel fluorescence microscopy technique for surface-generated fluorescence. The realized fluorescence microscope allows high resolution imaging of aqueous samples. The core element of the instrument is a parabolic mirror objective that is used to collect the fluorescence at large surface angles a...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Verdes D,Ruckstuhl T,Seeger S

    更新日期:2007-05-01 00:00:00

  • Real-time microscopic visualization of tissue response to laser thermal therapy.

    abstract::We present methods for visualizing the dynamic response of biological samples to laser-induced heating. Our approach utilizes optical frequency-domain imaging to detect, spatially localize, and monitor unique dynamic signatures that arise within zones of active tissue denaturation. Since this information is precisely ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Vakoc BJ,Tearney GJ,Bouma BE

    更新日期:2007-03-01 00:00:00

  • Autofluorescence of epithelial tissue: single-photon versus two-photon excitation.

    abstract::We instrumented a combined fluorescence spectroscopy and imaging system to characterize the single- and two-photon excited autofluorescence in epithelial tissue. Single-photon fluorescence (SPF) are compared with two-photon fluorescence (TPF) measured at the same location in epithelial tissue. It was found that the SP...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Zheng W,Wu Y,Li D,Qu JY

    更新日期:2008-09-01 00:00:00

  • Hybrid photomultiplier tube and photodiode parallel detection array for wideband optical spectroscopy of the breast guided by magnetic resonance imaging.

    abstract::A new optical parallel detection system of hybrid frequency and continuous-wave domains was developed to improve the data quality and accuracy in recovery of all breast optical properties. This new system was deployed in a previously existing system for magnetic resonance imaging (MRI)-guided spectroscopy, and allows ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: El-Ghussein F,Mastanduno MA,Jiang S,Pogue BW,Paulsen KD

    更新日期:2014-01-01 00:00:00

  • Optical delivery of liposome encapsulated chemical stimuli to neuronal cells.

    abstract::Spatially confined and precise time delivery of neuroactive molecules is an important issue in neurophysiology. In this work we developed a technique for delivering chemical stimuli to cultured neurons consisting in encapsulating the molecules of interest in liposomes. These vectors were then loaded in reservoirs cons...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Pinato G,Raffaelli T,D'Este E,Tavano F,Cojoc D

    更新日期:2011-09-01 00:00:00

  • Two-photon imaging using adaptive phase compensated ultrashort laser pulses.

    abstract::An adaptive pulse shaper controlled by multiphoton intrapulse interference phase scanning (MIIPS) was used, together with a prism-pair, to measure and cancel high-order phase distortions introduced by a high-numerical-aperture objective and other dispersive elements of a two-photon laser-scanning microscope. The deliv...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Xi P,Andegeko Y,Pestov D,Lovozoy VV,Dantus M

    更新日期:2009-01-01 00:00:00

  • In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence.

    abstract::Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selecti...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Orzekowsky-Schroeder R,Klinger A,Martensen B,Blessenohl M,Gebert A,Vogel A,Hüttmann G

    更新日期:2011-11-01 00:00:00

  • In vivo photoacoustic imaging of blood vessels using a homodyne interferometer with zero-crossing triggering.

    abstract::We demonstrate a quasinoncontact photoacoustic imaging method using a homodyne interferometer with a long coherence length laser. The generated photoacoustic signal is detected by a system that is locked at its maximum sensitivity through the use of balanced detection and zero-crossing triggering. The balanced detecto...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Lu J,Gao Y,Ma Z,Zhou H,Wang RK,Wang Y

    更新日期:2017-03-01 00:00:00

  • Nanophotothermolysis of multiple scattered cancer cells with carbon nanotubes guided by time-resolved infrared thermal imaging.

    abstract::Nanophotothermolysis with long laser pulses for treatment of scattered cancer cells and their clusters is introduced with the main focus on real-time monitoring of temperature dynamics inside and around individual cancer cells labeled with carbon nanotubes. This technique utilizes advanced time- and spatially-resolved...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Biris AS,Boldor D,Palmer J,Monroe WT,Mahmood M,Dervishi E,Xu Y,Li Z,Galanzha EI,Zharov VP

    更新日期:2009-03-01 00:00:00

  • Use of laser tweezers to analyze sperm motility and mitochondrial membrane potential.

    abstract::We combine laser tweezers with custom computer tracking software and robotics to analyze the motility [swimming speed, VCL (curvilinear velocity), and swimming force in terms of escape laser power (Pesc)] and energetics [mitochondrial membrane potential (MP)] of individual sperm. Domestic dog sperm are labeled with a ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Nascimento JM,Shi LZ,Chandsawangbhuwana C,Tam J,Durrant B,Botvinick EL,Berns MW

    更新日期:2008-01-01 00:00:00

  • Nondestructive inspection of phase transformation in zirconia-containing hip joints by confocal Raman spectroscopy.

    abstract::Environmental metastability of zirconia (ZrO2) ceramic in the human body [represented by a tetragonal-to-monoclinic (t→m) phase transformation] takes place on the surface of the artificial joint and proceeds with time toward its interior. Its quantitative characterization is mandatory for the safety of joint implants ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Zhu W,Sugano N,Pezzotti G

    更新日期:2013-12-01 00:00:00

  • Ultrasonic standing wave preparation of a liquid cell for glucose measurements in urine by midinfrared spectroscopy and potential application to smart toilets.

    abstract::Smart toilets could be used to monitor different components of urine in daily life for early detection of lifestyle-related diseases and prompt provision of treatment. For analysis of biological samples such as urine by midinfrared spectroscopy, thin-film samples like liquid cells are needed because of the strong abso...

    journal_title:Journal of biomedical optics

    pub_type: 信件


    authors: Yamamoto N,Kawashima N,Kitazaki T,Mori K,Kang H,Nishiyama A,Wada K,Ishimaru I

    更新日期:2018-05-01 00:00:00

  • On the noninvasive optical monitoring and differentiation of methemoglobinemia and sulfhemoglobinemia.

    abstract::There are several pathologies whose study and diagnosis is impaired by a relatively small number of documented cases. A practical approach to overcome this obstacle and advance the research in this area consists in employing computer simulations to perform controlled in silico experiments. The results of these experim...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章


    authors: Baranoski GV,Chen TF,Kimmel BW,Miranda E,Yim D

    更新日期:2012-09-01 00:00:00