FRET-based method for evaluation of the efficiency of reversible and irreversible sonoporation.

Abstract:

:It is widely known that not all of the treated cells survive after introduction of exogenous molecules via any physical method. Therefore, it is important to develop methods that would allow simultaneous evaluation of both molecular delivery efficiency and cell viability. This study presents Förster resonance energy transfer (FRET)-based method that allows molecular transfer and cell viability evaluation in a single measurement by employing two common fluorescent dyes, namely, ethidium bromide and trypan blue. The method has been validated using cell sonoporation. The FRET-based method allows the efficiency evaluation of both reversible and irreversible sonoporation in a single experiment. Therefore, this method could be used to reduce time, labor, and material cost while improving the accuracy of evaluations.

journal_name

J Biomed Opt

authors

Ruzgys P,Tamošiūnas M,Lukinsone V,Šatkauskas S

doi

10.1117/1.JBO.22.9.097001

subject

Has Abstract

pub_date

2017-09-01 00:00:00

pages

1-6

issue

9

eissn

1083-3668

issn

1560-2281

pii

JBO-170180R

journal_volume

22

pub_type

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