Abstract:
:It is widely known that not all of the treated cells survive after introduction of exogenous molecules via any physical method. Therefore, it is important to develop methods that would allow simultaneous evaluation of both molecular delivery efficiency and cell viability. This study presents Förster resonance energy transfer (FRET)-based method that allows molecular transfer and cell viability evaluation in a single measurement by employing two common fluorescent dyes, namely, ethidium bromide and trypan blue. The method has been validated using cell sonoporation. The FRET-based method allows the efficiency evaluation of both reversible and irreversible sonoporation in a single experiment. Therefore, this method could be used to reduce time, labor, and material cost while improving the accuracy of evaluations.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Ruzgys P,Tamošiūnas M,Lukinsone V,Šatkauskas Sdoi
10.1117/1.JBO.22.9.097001subject
Has Abstractpub_date
2017-09-01 00:00:00pages
1-6issue
9eissn
1083-3668issn
1560-2281pii
JBO-170180Rjournal_volume
22pub_type
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