Quantitative polarized Raman spectroscopy in highly turbid bone tissue.

abstract：:A transgenic mouse model with a Clim [co-factor of LIM (a combination of first letters of Lin-11 (C. elegans), ISL1 (rat), and Mec-3 (C. elegans) gene names) domain proteins] gene partially blocked in the epithelial compartment of its tissues is used to establish the sensitivity of intrinsic reflectance nonlinear opti...

journal_title：Journal of biomedical optics

authors： Lyubovitsky JG,Spencer JA,Krasieva TB,Andersen B,Tromberg BJ

更新日期：2006-01-01 00:00:00

abstract：SIGNIFICANCE:Despite the importance of the cell membrane in regulation of drug activity, the influence of drug treatments on its physical properties is still poorly understood. The combination of fluorescence lifetime imaging microscopy (FLIM) with specific viscosity-sensitive fluorescent molecular rotors allows the qu...

journal_title：Journal of biomedical optics

authors： Shimolina LE,Gulin AA,Paez-Perez M,López-Duarte I,Druzhkova IN,Lukina MM,Gubina MV,Brooks NJ,Zagaynova EV,Kuimova MK,Shirmanova MV

更新日期：2020-12-01 00:00:00

abstract：:The feasibility of spatial frequency domain imaging (SFDI) for breast surgical margin assessment was evaluated in tissue-simulating phantoms and in fully intact lumpectomy specimens at the time of surgery. Phantom data was evaluated according to contrast-detail resolution, quantitative accuracy and model-data goodness...

journal_title：Journal of biomedical optics

authors： Laughney AM,Krishnaswamy V,Rice TB,Cuccia DJ,Barth RJ,Tromberg BJ,Paulsen KD,Pogue BW,Wells WA

更新日期：2013-03-01 00:00:00

abstract：:Selective retina treatment (SRT) is a novel short pulsed laser therapy of several retinal diseases associated with a decreased metabolism at the retinal pigment epithelium (RPE). The range of laser pulse energies is small, in which the desired selective RPE disintegration is achieved without adverse effects to the neu...

journal_title：Journal of biomedical optics

authors： Neumann J,Brinkmann R

更新日期：2006-07-01 00:00:00

abstract：:We introduce the application of functional data analysis (fDA) on functional near-infrared spectroscopy (fNIRS) signals for the development of an accurate and clinically practical assessment method of pain perception. We used the cold pressor test to induce different levels of pain in healthy subjects while the fNIRS ...

journal_title：Journal of biomedical optics

authors： Pourshoghi A,Zakeri I,Pourrezaei K

更新日期：2016-10-01 00:00:00

abstract：:Noninvasive cardiovascular imaging could lead to the early detection and timely treatment of complex atherosclerotic lesions responsible for major cardiovascular events. Recent investigations have suggested that optical coherence tomography (OCT) is an ideal diagnostic tool due to the high resolution this technology a...

journal_title：Journal of biomedical optics

authors： Ghosn MG,Carbajal EF,Befrui NA,Tellez A,Granada JF,Larin KV

更新日期：2008-01-01 00:00:00

abstract：:A photoacoustic imaging system that incorporates a commercial ultrasonic camera for real-time imaging of two-dimensional (2-D) projection planes in tissue at video rate (30 Hz) is presented. The system uses a Q-switched frequency-doubled Nd:YAG pulsed laser for photoacoustic generation. The ultrasonic camera consists ...

journal_title：Journal of biomedical optics

authors： Balogun O,Regez B,Zhang HF,Krishnaswamy S

更新日期：2010-03-01 00:00:00

abstract：:This study investigates the precision and intraindividual variability of a clinical optical pachometer based on low-coherence reflectometry, which was used to measure the central thickness of a human cornea in vivo. The instrument, attached to a slit lamp, is a single mode fiber optic based Michelson interferometer wi...

journal_title：Journal of biomedical optics

authors： Bo Hnke M,Masters BR,Wa Lti R,Ballif JJ,Chavanne P,Gianotti R,Salathe RP

更新日期：1999-01-01 00:00:00

abstract：:A side-viewing, 2.3-mm diameter, surface magnifying chromoendoscopy-optical coherence tomography (SMC-OCT) endoscope has been designed for simultaneous, nondestructive surface fluorescence visualization and cross-sectional imaging. We apply this endoscope to in vivo examination of the mouse colon. A 30,000 element fib...

journal_title：Journal of biomedical optics

authors： Wall RA,Barton JK

更新日期：2012-08-01 00:00:00

abstract：:The specificity of molecular and functional photoacoustic (PA) images depends on the accuracy of the photoacoustic absorption spectroscopy. The PA signal is proportional to the product of the optical absorption coefficient and local light fluence; quantitative PA measurements of the optical absorption coefficient ther...

journal_title：Journal of biomedical optics

authors： Bauer AQ,Nothdurft RE,Erpelding TN,Wang LV,Culver JP

更新日期：2011-09-01 00:00:00

abstract：:The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the micro...

journal_title：Journal of biomedical optics

authors： Doblas A,Sánchez-Ortiga E,Martínez-Corral M,Saavedra G,Garcia-Sucerquia J

更新日期：2014-04-01 00:00:00

abstract：:Alcohol testing is an expanding area of interest due to the impacts of alcohol abuse that extend well beyond drunk driving. However, existing approaches such as blood and urine assays are hampered in some testing environments by biohazard risks. A noninvasive, in vivo spectroscopic technique offers a promising alterna...

journal_title：Journal of biomedical optics

authors： Ridder TD,Ver Steeg BJ,Laaksonen BD

更新日期：2009-09-01 00:00:00

abstract：:The infrared and Raman spectroscopy of bone and teeth tissues are reviewed. Characteristic spectra are obtained for both the mineral and protein components of these tissues. Vibrational spectroscopy is used to study the mineralization process, to define the chemical structure changes accompanying bone diseases, and to...

journal_title：Journal of biomedical optics

authors： Carden A,Morris MD

更新日期：2000-07-01 00:00:00

abstract：:Doxorubicin is a commonly used chemotherapeutic employed to treat multiple human cancers, including numerous sarcomas and carcinomas. Furthermore, doxorubicin possesses strong fluorescent properties that make it an ideal reagent for modeling drug delivery by examining its distribution in cells and tissues. However, wh...

journal_title：Journal of biomedical optics

authors： Carlson M,Watson AL,Anderson L,Largaespada DA,Provenzano PP

更新日期：2017-11-01 00:00:00

abstract：:Using single-molecule microscopy, we present a method to quantify the number of single autofluorescent proteins when they cannot be optically resolved. This method relies on the measurement of the total intensity emitted by each aggregate until it photobleaches. This strategy overcomes the inherent problem of blinking...

journal_title：Journal of biomedical optics

authors： Cognet L,Tardin C,Négrier ML,Breillat C,Coussen F,Choquet D,Lounis B

更新日期：2008-05-01 00:00:00

abstract：:We successfully acquire the in vivo Raman spectrum of melanin from human skin using a rapid near-infrared (NIR) Raman spectrometer. The Raman signals of in vivo cutaneous melanin are similar to those observed from natural and synthetic eumelanins. The melanin Raman spectrum is dominated by two intense and broad peaks ...

journal_title：Journal of biomedical optics

authors： Huang Z,Lui H,Chen XK,Alajlan A,McLean DI,Zeng H

更新日期：2004-11-01 00:00:00

abstract：:Pilot results on the application of the finite-difference time-domain (FDTD) approach for studying the implementation of the optical immersion technique for the visualization of single and multiple gold nanoparticles in biological cells are presented. We focus on two different scenarios considering single biological c...

journal_title：Journal of biomedical optics

authors： Tanev S,Tuchin VV,Paddon P

更新日期：2006-11-01 00:00:00

abstract：:Quantification of nicotinamide adenine dinucleotide (NADH) changes during functional brain activation and pathological conditions provides critical insight into brain metabolism. Of the different imaging modalities, two-photon laser scanning microscopy (TPLSM) is becoming an important tool for cellular-resolution meas...

journal_title：Journal of biomedical optics

authors： Baraghis E,Devor A,Fang Q,Srinivasan VJ,Wu W,Lesage F,Ayata C,Kasischke KA,Boas DA,Sakadzić S

更新日期：2011-10-01 00:00:00

abstract：:We present a multimodal optical microscope that incorporates six imaging modalities on one common platform. The imaging modalities include three staring modes, optical quadrature microscopy (OQM), differential interference contrast (DIC) microscopy, and epi-fluorescence microscopy, and three scanning modes, confocal r...

journal_title：Journal of biomedical optics

authors： Warger WC 2nd,Laevsky GS,Townsend DJ,Rajadhyaksha M,DiMarzio CA

更新日期：2007-07-01 00:00:00

abstract：:Sinus blockages are a common reason for physician visits, affecting one out of seven people in the United States, and often require medical treatment. Diagnosis in the primary care setting is challenging because symptom criteria (via detailed clinical history) plus objective imaging [computed tomography (CT) or endosc...

journal_title：Journal of biomedical optics

authors： Cerussi AE,Mishra N,You J,Bhandarkar N,Wong B

更新日期：2015-03-01 00:00:00

abstract：:Second-harmonic-generation (SHG) microscopy has emerged as a powerful tool to image unstained living tissues and probe their molecular and supramolecular organization. In this article, we review the physical basis of SHG, highlighting how coherent summation of second-harmonic response leads to the sensitivity of polar...

journal_title：Journal of biomedical optics

authors： Vanzi F,Sacconi L,Cicchi R,Pavone FS

更新日期：2012-06-01 00:00:00

abstract：:A new optical parallel detection system of hybrid frequency and continuous-wave domains was developed to improve the data quality and accuracy in recovery of all breast optical properties. This new system was deployed in a previously existing system for magnetic resonance imaging (MRI)-guided spectroscopy, and allows ...

journal_title：Journal of biomedical optics

authors： El-Ghussein F,Mastanduno MA,Jiang S,Pogue BW,Paulsen KD

更新日期：2014-01-01 00:00:00

abstract：:Confocal micro-Raman spectroscopy--a valuable analytical tool in biological and medical field of research-allows probing molecular vibrations of samples without external labels or extensive preparation. We employ confocal micro-Raman spectroscopy to characterize renal tumors and normal tissue. Results show that Raman ...

journal_title：Journal of biomedical optics

authors： Zhuang Z,Li N,Guo Z,Zhu M,Xiong K,Chen S

更新日期：2013-03-01 00:00:00

abstract：:We assess the properties of contact pressure applied by manually operated fiber-optic probes as a function of the operator, probe contact area, and sample stiffness. First, the mechanical properties of human skin sites with different skin structures, thicknesses, and underlying tissues were studied by in vivo indentat...

journal_title：Journal of biomedical optics

authors： Bregar M,Cugmas B,Naglic P,Hartmann D,Pernuš F,Likar B,Bürmen M

更新日期：2015-01-01 00:00:00

abstract：:We present functional imaging of the concentration of a photodynamic therapy (PDT)-related dye in scattering tissue phantoms based on spatially resolved measurements of optical properties through spectroscopic optical coherence tomography (OCT). Expressions for the OCT signal are developed, enabling estimation of dept...

journal_title：Journal of biomedical optics

authors： Støren T,Røyset A,Svaasand LO,Lindmo T

更新日期：2005-03-01 00:00:00

abstract：:A simultaneous multispectral fluorescence imaging system incorporating multiplexed volume holographic grating (VHG) is developed to acquire multispectral images of an object in one shot. With the multiplexed VHG, the imaging system can provide the distribution and spectral characteristics of multiple fluorophores in t...

journal_title：Journal of biomedical optics

authors： Lv Y,Zhang J,Zhang D,Cai W,Chen N,Luo J

更新日期：2016-06-01 00:00:00

abstract：:Ambulatory diffuse optical tomography (aDOT) is based on near-infrared spectroscopy (NIRS) and enables three-dimensional imaging of regional hemodynamics and oxygen consumption during a person’s normal activities. Although NIRS has been previously used for muscle assessment, it has been notably limited in terms of the...

journal_title：Journal of biomedical optics

authors： Hu G,Zhang Q,Ivkovic V,Strangman GE

更新日期：2016-09-01 00:00:00

abstract：:We demonstrate a quasinoncontact photoacoustic imaging method using a homodyne interferometer with a long coherence length laser. The generated photoacoustic signal is detected by a system that is locked at its maximum sensitivity through the use of balanced detection and zero-crossing triggering. The balanced detecto...

journal_title：Journal of biomedical optics

authors： Lu J,Gao Y,Ma Z,Zhou H,Wang RK,Wang Y

更新日期：2017-03-01 00:00:00

abstract：:We investigated the effect of thiazone, a widely used penetration enhancer, on in vitro porcine skin and muscle tissue by single-integrating sphere technique during optical clearing (OC) treatment. The results showed that thiazone induced an increase on the total transmittance of skin which led to a reduction in that ...

journal_title：Journal of biomedical optics

authors： Jin X,Deng Z,Wang J,Ye Q,Mei J,Zhou W,Zhang C,Tian J

更新日期：2016-10-01 00:00:00

abstract：:We recently developed an algorithm for multiplexed fluorescence tomographic imaging of at least four fluorophores concurrently in the red and near-infrared wavelength region by jointly using spectral and temporal data. We report the design of a fluorescence tomography instrument that acquires spectral and temporal dat...

journal_title：Journal of biomedical optics

authors： Mu Y,Pera V,Niedre M

更新日期：2016-10-01 00:00:00