Abstract:
:We recently developed an algorithm for multiplexed fluorescence tomographic imaging of at least four fluorophores concurrently in the red and near-infrared wavelength region by jointly using spectral and temporal data. We report the design of a fluorescence tomography instrument that acquires spectral and temporal data, and validate its use in tissue-mimicking phantoms with four embedded fluorescent targets with highly overlapped spectral signatures. Critically, this requires measurement or computation of extended fluorophore signature libraries, which capture the variability in the measured signal due to the unknown position of the targets in the media. We demonstrate that we can demix and tomographically image all four fluorophores with zero image cross-talk, and 1 mm or better spatial resolution.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Mu Y,Pera V,Niedre Mdoi
10.1117/1.JBO.21.10.105001subject
Has Abstractpub_date
2016-10-01 00:00:00pages
105001issue
10eissn
1083-3668issn
1560-2281pii
2565809journal_volume
21pub_type
杂志文章abstract::A need exists for the continued development of diagnostic tools and methods capable of distinguishing and characterizing slight differences in the optical properties of tissues. We present a method to estimate the scattering coefficient contribution as a function of particle size in complex mixtures of polystyrene sph...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2363347
更新日期:2006-09-01 00:00:00
abstract::Microvascular network of the retina plays an important role in diagnosis and monitoring of various retinal diseases. We propose a three-dimensional (3-D) segmentation method with intensity-based Doppler variance (IBDV) based on swept-source optical coherence tomography. The automatic 3-D segmentation method is used to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.7.076003
更新日期:2015-07-01 00:00:00
abstract::The application of near-infrared spectroscopy (NIRS) to assess microvascular function has shown promising results. An important limitation when using a single source-detector pair, however, is the lack of depth sensitivity. Diffuse optical tomography (DOT) overcomes this limitation using an array of sources and detect...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.6.066012
更新日期:2016-06-01 00:00:00
abstract::We experimentally implement label-free phase microscopy using wide-field digital interferometry (WFDI) techniques to retrieve quantitative volumetric data of articular chondrocyte dynamics. Using the scanless interferometric system, we visualize chondrocyte swelling and bursting induced by hypo-osmotic pressure. Recon...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3290242
更新日期:2010-01-01 00:00:00
abstract::Nonviral, site-specific gene delivery to deep tissue is required for gene therapy of a spinal cord injury. However, an efficient method satisfying these requirements has not been established. This study demonstrates efficient and targeted gene transfer into the spinal cord by using photomechanical waves (PMWs), which ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3642014
更新日期:2011-10-01 00:00:00
abstract::Skin cancer diagnosis depends not only on histopathological examination but also on visual inspection before and after the excision of suspected lesion. Neoplasm is accompanied with changes in birefringence of collagen, pleomorphicity, and hyperchromatic state of epithelial nuclei. These phenomena can be measured by s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3609003
更新日期:2011-08-01 00:00:00
abstract::The authors' aim is to assess and quantitatively measure brain hemodynamic and morphological variations during closed-head injury (CHI) in mice using orthogonal diffuse near-infrared reflectance spectroscopy (o-DRS). CHI is a type of injury to the head that does not penetrate the skull. Usually, it is caused by mechan...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.4.045003
更新日期:2013-04-01 00:00:00
abstract::Optically derived tissue properties across a range of breast densities and the effects of breast compression on estimates of hemoglobin, oxygen metabolism, and water and lipid concentrations were obtained from a coregistered imaging system that integrates near-infrared spectral tomography (NIRST) with digital breast t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.9.091316
更新日期:2016-09-01 00:00:00
abstract::Imaging large areas of tissue rapidly and with high resolution may enable rapid pathology at the bedside. The limited field of view of high-resolution microscopes requires the merging of multiple images that are taken sequentially to cover a large area. This merging or mosaicing of images requires long acquisition and...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3582335
更新日期:2011-05-01 00:00:00
abstract::We have developed a novel parallel-plate diffuse optical tomography (DOT) system for three-dimensional in vivo imaging of human breast tumor based on large optical data sets. Images of oxy-, deoxy-, and total hemoglobin concentration as well as blood oxygen saturation and tissue scattering were reconstructed. Tumor ma...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3103325
更新日期:2009-03-01 00:00:00
abstract::Diffuse optical imaging is an effective technique for noninvasive functional brain imaging. However, the measurements respond to systemic hemodynamic fluctuations caused by the cardiac cycle, respiration, and blood pressure, which may obscure or overwhelm the desired stimulus-evoked response. Previous work on this pro...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1852552
更新日期:2005-01-01 00:00:00
abstract::We investigate optoelectronic properties of integrated structures comprising semiconductor light-emitting materials for optical probes of microscopic biological systems. Compound semiconductors are nearly ideal light emitters for probing cells and other microorganisms because of their spectral match to the transparenc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1506931
更新日期:2002-10-01 00:00:00
abstract::Intraductal carcinoma is a precancerous lesion of the breast and the immediate precursor of invasive ductal carcinoma. Multiphoton microscopy (MPM) was used to monitor the progression from intraductal carcinoma to invasive ductal carcinoma, which can improve early detection of precursor lesions and halt progression to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.9.096007
更新日期:2015-09-01 00:00:00
abstract::In order to understand the relationship between accommodation and vision quality, a custom-built ultra-long scan depth spectral domain optical coherence tomography (UL-SDOCT) and a Shack-Hartmann wavefront sensor (HSWFS) were combined. The resolution and scan depth of UL-SDOCT are 6 μm and 15.6 mm, respectively, which...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.JBO.17.12.120501
更新日期:2012-12-01 00:00:00
abstract::A swept source (SS)-based circular-state (CS) polarization-sensitive optical coherence tomography (PS-OCT) constructed entirely with polarization-maintaining fiber optics components is proposed with the experimental verification. By means of the proposed calibration scheme, bulk quarter-wave plates can be replaced by ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.2.021110
更新日期:2014-02-01 00:00:00
abstract::Optical quantification of large lesions imaged with diffuse optical tomography in reflection geometry is depth dependence due to the exponential decay of photon density waves. We introduce a depth-correction method that incorporates the target depth information provided by coregistered ultrasound. It is based on balan...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3573814
更新日期:2011-05-01 00:00:00
abstract::We present the first experimental result of direct delineation of the nuclei of living rat bladder epithelium with ultrahigh-resolution optical coherence tomography (uOCT). We demonstrate that the cellular details embedded in the speckle noise in a uOCT image can be uncovered by time-lapse frame averaging that takes a...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.2800007
更新日期:2007-09-01 00:00:00
abstract::We investigate and assess the utility of a simple scheme for continuous absolute blood flow monitoring based on diffuse correlation spectroscopy (DCS). The scheme calibrates DCS using venous-occlusion diffuse optical spectroscopy (VO-DOS) measurements of arm muscle tissue at a single time-point. A calibration coeffici...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.12.125005
更新日期:2015-01-01 00:00:00
abstract::We experimentally compare the performance and image contrast of spontaneous Raman and coherent Stokes Raman scattering microscopy. We demonstrate the differences between these techniques on a series of geometry-controlled samples that range in complexity from a point (array of tips) to one-dimensional (line grating) a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3533310
更新日期:2011-02-01 00:00:00
abstract::Lumpectomy coupled with radiation therapy and/or chemotherapy is commonly used to treat breast cancer patients. We are developing an enhanced thermal IR imaging technique that has the potential to provide real-time imaging to guide tissue excision during a lumpectomy by delineating tumor margins. This enhanced thermal...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.11.116003
更新日期:2015-11-01 00:00:00
abstract::Cerenkov light is created in clinical applications involving high-energy radiation such as in radiation therapy. There is considerable interest in using Cerenkov light as a means to perform in vivo dosimetry during radiation therapy; however, a better understanding of the light-to-dose relationship is needed. One such...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.10.105007
更新日期:2018-10-01 00:00:00
abstract::Artificial neural stimulation is widely used in clinic, rehabilitation, and research. One of the limitations of electrical stimulation is the current spread in tissue. Recently, pulsed mid-infrared laser stimulation of nerves has been investigated as an alternative stimulation method. The likely benefits of infrared n...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3655590
更新日期:2011-11-01 00:00:00
abstract::Collagen fibers can be imaged with second harmonic generation (SHG) and are associated with efficient tumor cell locomotion. Preferential locomotion along these fibers correlates with a more aggressively metastatic phenotype, and changes in SHG emission properties accompany changes in metastatic outcome. We therefore ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.8.086003
更新日期:2013-08-01 00:00:00
abstract::Ophthalmic surgery may benefit from use of more precise fiber delivery systems during laser surgery. Some current ophthalmic surgical techniques rely on tedious mechanical dissection of tissue layers. In this study, chains of sapphire microspheres integrated into a hollow waveguide distal tip are used for erbium:YAG l...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.6.068004
更新日期:2012-06-01 00:00:00
abstract:SIGNIFICANCE:Melanoma is a deadly cancer that physicians struggle to diagnose early because they lack the knowledge to differentiate benign from malignant lesions. Deep machine learning approaches to image analysis offer promise but lack the transparency to be widely adopted as stand-alone diagnostics. AIM:We aimed to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.11.112906
更新日期:2020-11-01 00:00:00
abstract::The formation of kidney stones is a common and highly studied disease, which causes intense pain and presents a high recidivism. In order to find the causes of this problem, the characterization of the main compounds is of great importance. In this sense, the analysis of the composition and structure of the stone can ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.076027
更新日期:2012-07-01 00:00:00
abstract::Clustering analysis (CA) and principal component analysis (PCA) were applied to dynamic Cerenkov luminescence images (dCLI). In order to investigate the performances of the proposed approaches, two distinct dynamic data sets obtained by injecting mice with (32)P-ATP and (18)F-FDG were acquired using the IVIS 200 optic...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3663442
更新日期:2011-12-01 00:00:00
abstract::We have presented an innovative laser-assisted reduction surgery (LARS) based on plasma-induced ablation and photodisruption effects. In addition, we developed a laser operation system. Fetuses of mice from the Institute for Cancer Research that were immersed in physiological saline were irradiated by convergent-pulse...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.11.118002
更新日期:2013-11-01 00:00:00
abstract::Functional near-infrared spectroscopy (fNIRS) is being increasingly applied to affective and social neuroscience research; however, the reliability of this method is still unclear. This study aimed to evaluate the test–retest reliability of the fNIRS-based prefrontal response to emotional stimuli. Twenty-six participa...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.1.016011
更新日期:2017-01-01 00:00:00
abstract::Most ovarian cancers are diagnosed at advanced stages due to the lack of efficacious screening techniques. Photoacoustic tomography (PAT) has a potential to image tumor angiogenesis and detect early neovascular changes of the ovary. We have developed a coregistered PAT and ultrasound (US) prototype system for real-tim...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.4.046006
更新日期:2016-04-30 00:00:00