Abstract:
SIGNIFICANCE:Despite the importance of the cell membrane in regulation of drug activity, the influence of drug treatments on its physical properties is still poorly understood. The combination of fluorescence lifetime imaging microscopy (FLIM) with specific viscosity-sensitive fluorescent molecular rotors allows the quantification of membrane viscosity with high spatiotemporal resolution, down to the individual cell organelles. AIM:The aim of our work was to analyze microviscosity of the plasma membrane of living cancer cells during chemotherapy with cisplatin using FLIM and correlate the observed changes with lipid composition and cell's response to treatment. APPROACH:FLIM together with viscosity-sensitive boron dipyrromethene-based fluorescent molecular rotor was used to map the fluidity of the cell's membrane. Chemical analysis of membrane lipid composition was performed with time-of-flight secondary ion mass spectrometry (ToF-SIMS). RESULTS:We detected a significant steady increase in membrane viscosity in viable cancer cells, both in cell monolayers and tumor spheroids, upon prolonged treatment with cisplatin, as well as in cisplatin-adapted cell line. ToF-SIMS revealed correlative changes in lipid profile of cisplatin-treated cells. CONCLUSIONS:These results suggest an involvement of membrane viscosity in the cell adaptation to the drug and in the acquisition of drug resistance.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Shimolina LE,Gulin AA,Paez-Perez M,López-Duarte I,Druzhkova IN,Lukina MM,Gubina MV,Brooks NJ,Zagaynova EV,Kuimova MK,Shirmanova MVdoi
10.1117/1.JBO.25.12.126004subject
Has Abstractpub_date
2020-12-01 00:00:00issue
12eissn
1083-3668issn
1560-2281pii
JBO-200248Rjournal_volume
25pub_type
杂志文章abstract::Two-photon excitation microscopy is one of the key techniques used to observe three-dimensional (3-D) structures in biological samples. We utilized a visible-wavelength laser beam for two-photon excitation in a multifocus confocal scanning system to improve the spatial resolution and image contrast in 3-D live-cell im...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.1.014502
更新日期:2019-11-01 00:00:00
abstract::The capability of optical coherence tomography (OCT) to perform "optical biopsy" of tissues within a depth range of 1 to 2 mm with micron-scale resolution in real time makes it a promising biomedical imaging modality for dermatologic applications. Three high-speed, spectrometer-based frequency-domain OCT systems opera...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3400665
更新日期:2010-03-01 00:00:00
abstract::Nonmelanoma skin cancer is the most common form of human cancer, often resulting in high morbidity. Low visual contrast of these tumors makes their delineation a challenging problem. Employing a linearly polarized monochromatic light source and a wide-field CCD camera, we have developed a technique for fluorescence po...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2435710
更新日期:2007-01-01 00:00:00
abstract::We present a novel Fourier-domain angle-resolved low-coherence interferometry (a /LCI) fiber probe designed for in vivo clinical application in gastrointestinal endoscopy. The a/LCI technique measures the depth-resolved angular scattering distribution to determine the size distribution and optical density of cell nucl...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3520130
更新日期:2011-01-01 00:00:00
abstract::Reactive oxygen species (ROS) produced by photodynamic therapy (PDT) is recorded in vivo using a chemiluminescence (CL)-based gated optical system. A novel approach is developed to utilize the fluorescence (FL) of the CL probe as an internal fluorescence to calibrate the observed CL on pharmacokinetics of the probe in...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3368688
更新日期:2010-03-01 00:00:00
abstract::The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the micro...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.4.046022
更新日期:2014-04-01 00:00:00
abstract::A model that describes the concentration of photothermal (light-to-heat converters) compounds as a function of depth in a turbid medium is developed. The system consists of a pump laser (808 nm modulated at 400 Hz), which heats a photothermal compound, and a phase sensitive spectral domain optical coherence tomography...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3659211
更新日期:2011-12-01 00:00:00
abstract::A Fourier transform spectrometer is used to simultaneously measure distance, dispersion and spectrum. It is shown that short coherence interferometry has the potential to measure the three-dimensional distribution of the spatial structure of a sample with a resolution determined by the coherence length of the light so...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429885
更新日期:1998-01-01 00:00:00
abstract::We measure the tumor vascular response to varying irradiance rates during photodynamic therapy (PDT) in a Dunning rat prostate model with interstitial Doppler optical coherence tomography (IS-DOCT). Rats are given a photosensitizer drug, Photofrin, and the tumors are exposed to light (635 nm) with irradiance rates ran...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2744068
更新日期:2007-05-01 00:00:00
abstract::We describe a wide-field optical tomography technique, which allows the measurement-guided optimization of illumination patterns for enhanced reconstruction performances. The iterative optimization of the excitation pattern aims at reducing the dynamic range in photons transmitted through biological tissue. It increas...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.3.036006
更新日期:2013-03-01 00:00:00
abstract::Different colors of visible light penetrate to varying depths in tissue due to the wavelength dependence of tissue optical absorption and elastic scattering. We exploit this to map the contour of the closest surface of a buried fluorescent object. This uses a novel algorithm based on the diffusion theory description o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3523369
更新日期:2010-11-01 00:00:00
abstract::Muscle blood flow (MBF) and muscle oxygen saturation (SmO(2)) were measured at eight locations (four proximal, four distal) over a 4 x 8 cm(2) area of the vastus lateralis at rest and immediately after isometric, maximal quadriceps contraction using multichannel, frequency-domain, near-infrared spectroscopy. A venous ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1646417
更新日期:2004-03-01 00:00:00
abstract::A hyperspectral image projector (HIP) based on liquid crystal on silicon spatial light modulators is explained and demonstrated to generate data cubes. The HIP-constructed data cubes are three-dimensional images of the spatial distribution of spectrally resolved abundances of intracellular light-absorbing oxyhemoglobi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,收录出版
doi:10.1117/1.JBO.20.11.111215
更新日期:2015-01-01 00:00:00
abstract::The essential feature of the confocal laser scanning microscope (cLSM) is the generation of optical sections by the removal of out-of-focus light. About ten years ago, structured illumination microscopy (SIM) was introduced as an alternative method for obtaining optical sections from biological specimens. Here we comp...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3083439
更新日期:2009-01-01 00:00:00
abstract::A simple empirical method is presented to determine the scattering coefficient mu' s from backscattered polarized images of turbid media. It uses the ratio, pixel by pixel, of two images that are the second and the first backscattered Stokes parameter images Q and I, respectively. Taking this image ratio, then integra...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1924715
更新日期:2005-05-01 00:00:00
abstract::In the last 25 years, optical coherence tomography (OCT) has advanced to be one of the most innovative and most successful translational optical imaging techniques, achieving substantial economic impact as well as clinical acceptance. This is largely owing to the resolution improvements by a factor of 10 to the submic...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.JBO.19.7.071412
更新日期:2014-01-01 00:00:00
abstract::Selective retina treatment (SRT) is a novel short pulsed laser therapy of several retinal diseases associated with a decreased metabolism at the retinal pigment epithelium (RPE). The range of laser pulse energies is small, in which the desired selective RPE disintegration is achieved without adverse effects to the neu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2339815
更新日期:2006-07-01 00:00:00
abstract::Monitoring a degranulation process in a live mast cell is a quite important issue in immunology and pharmacology. Because the size of a granule is normally much smaller than the resolution limit of an optical microscope system, there is no direct real-time live cell imaging technique for observing degranulation proces...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3470104
更新日期:2010-07-01 00:00:00
abstract::Focusing light inside highly scattering media is a challenging task in biomedical optical imaging, manipulation, and therapy. A recent invention has overcome this challenge by time reversing ultrasonically encoded diffuse light to an ultrasound-modulated volume inside a turbid medium. In this technique, a photorefract...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.11.110502
更新日期:2013-11-01 00:00:00
abstract::Discrimination of benign and malignant melanocytic lesions is a major issue in clinical dermatology. Assessment of the thickness of melanoma is critical for prognosis and treatment selection. We aimed to evaluate a novel optical computed tomography (optical-CT) system as a tool for three-dimensional (3-D) imaging of m...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.6.066004
更新日期:2012-06-01 00:00:00
abstract::Amblyopia is a form of visual impairment caused by ocular misalignment (strabismus) or defocus in an otherwise healthy eye. If detected early, the condition can be fully treated, yet over half of all children with amblyopia under age 5 escape detection. We developed a Pediatric Vision Screener (PVS) to detect amblyopi...
journal_title:Journal of biomedical optics
pub_type: 临床试验,杂志文章
doi:10.1117/1.1805561
更新日期:2004-11-01 00:00:00
abstract::Blood smear analysis has remained a crucial diagnostic tool for pathologists despite the advent of automatic analyzers such as flow cytometers and impedance counters. Though these current methods have proven to be indispensible tools for physicians and researchers alike, they provide limited information on the detaile...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3369965
更新日期:2010-03-01 00:00:00
abstract::Temperature distribution is a crucial factor in determining the outcome of laser phototherapy in cancer treatment. Magnetic resonance imaging (MRI) is an ideal method for 3-D noninvasive temperature measurement. A 7.1-T MRI was used to determine laser-induced high thermal gradient temperature distribution of target ti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2960020
更新日期:2008-07-01 00:00:00
abstract::Fourier transform infrared (FTIR) microspectroscopy has been used to estimate the spatial proteoglycan (PG) and collagen contents in articular cartilage (AC). However, it is not clear whether the results of FTIR analyses are consistent between different species. Our aim was to clarify how three different FTIR PG param...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.9.097006
更新日期:2013-09-01 00:00:00
abstract::Spatially resolved spectroscopy (SRS) allows the estimation of absolute tissue oxygen saturation, the ratio of oxygenated to total hemoglobin concentration, which may facilitate the comparison of results among patients. Eighty-two premature infants were included over two years. The cerebral tissue oxygenation index (c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3013454
更新日期:2008-11-01 00:00:00
abstract::Color Doppler optical coherence tomography (CDOCT) is a functional extension of optical coherence tomography (OCT) that can image flow in turbid media. We have developed a CDOCT system capable of imaging flow in real time. Doppler processing of the analog signal is accomplished in hardware in the time domain using a n...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1428291
更新日期:2002-01-01 00:00:00
abstract::Metal nanoparticles can be functionalized with biomolecules to selectively localize in precancerous tissues and can act as optical contrast enhancers for reflectance-based diagnosis of epithelial precancer. We carry out Monte Carlo (MC) simulations to analyze photon propagation through nanoparticle-labeled tissues and...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3608999
更新日期:2011-08-01 00:00:00
abstract:SIGNIFICANCE:The retina is critical for vision, and several diseases may alter its biomechanical properties. However, assessing the biomechanical properties of the retina nondestructively is a challenge due to its fragile nature and location within the eye globe. Advancements in Brillouin spectroscopy have provided the...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.9.090502
更新日期:2020-09-01 00:00:00
abstract::Ex vivo human skin has been used extensively for cosmeceutical and drug delivery studies, transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to ischemic necrosis. Traditional methods of assessing viability can be time-consuming and provide limited metabolic information. Using ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3466580
更新日期:2010-07-01 00:00:00
abstract::Parametric and nonparametric data processing schemes for analyzing translating laser speckle data used to investigate the mechanical behavior of biological tissues are examined. Cross-correlation, minimum mean square estimator, maximum likelihood, and maximum entropy approaches are discussed and compared on speckle da...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1412224
更新日期:2001-10-01 00:00:00