Protein conformation and molecular order probed by second-harmonic-generation microscopy.

abstract：:A highly efficient method based on Maxwell's theory was developed, which enables the calculation of the scanning of a focused beam through scattering media. Maxwell's equations were numerically solved in two dimensions using finite difference time domain simulations. The modeling of the focused beam was achieved by ap...

journal_title：Journal of biomedical optics

authors： Elmaklizi A,Schäfer J,Kienle A

更新日期：2014-07-01 00:00:00

abstract：:Deep anterior lamellar keratoplasty (DALK) is an emerging surgical technique for the restoration of corneal clarity and vision acuity. The big-bubble technique in DALK surgery is the most essential procedure that includes the air injection through a thin syringe needle to separate the dysfunctional region of the corne...

journal_title：Journal of biomedical optics

authors： Shin S,Bae JK,Ahn Y,Kim H,Choi G,Yoo YS,Joo CK,Moon S,Jung W

更新日期：2017-12-01 00:00:00

abstract：:Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries res...

journal_title：Journal of biomedical optics

authors： Yao J,Maslov K,Hu S,Wang LV

更新日期：2009-09-01 00:00:00

abstract：:The capability of optical coherence tomography (OCT) to perform "optical biopsy" of tissues within a depth range of 1 to 2 mm with micron-scale resolution in real time makes it a promising biomedical imaging modality for dermatologic applications. Three high-speed, spectrometer-based frequency-domain OCT systems opera...

journal_title：Journal of biomedical optics

authors： Alex A,Povazay B,Hofer B,Popov S,Glittenberg C,Binder S,Drexler W

更新日期：2010-03-01 00:00:00

abstract：:Recently, it was shown in measurements carried out on humans that time-resolved near-infrared reflectometry and fluorescence spectroscopy may allow for discrimination of information originating directly from the brain avoiding influence of contaminating signals related to the perfusion of extracerebral tissues. We rep...

journal_title：Journal of biomedical optics

authors： Liebert A,Sawosz P,Milej D,Kacprzak M,Weigl W,Botwicz M,Maczewska J,Fronczewska K,Mayzner-Zawadzka E,Królicki L,Maniewski R

更新日期：2011-04-01 00:00:00

abstract：:Fiber-guided ablation of soft tissue with pulsed holmium and thulium lasers was investigated for intraluminal incisions. A bare fiber/tissue-contact application system with a nearly tangential irradiation geometry was first used in vitro on porcine ureter tissue. The efficiency and precision of the method was analyzed...

journal_title：Journal of biomedical optics

authors： Brinkmann R,Knipper A,Dro Ge G,Schro Er F,Gromoll B,Birngruber R

更新日期：1998-01-01 00:00:00

abstract：:The composition of cartilage is predictive of its in vivo performance. Therefore, the ability to assess its primary macromolecular components, proteoglycan (PG) and collagen, is of great importance. In the current study, we hypothesized that PG content and distribution in tissue engineered cartilage could be determine...

journal_title：Journal of biomedical optics

authors： Kim M,Bi X,Horton WE,Spencer RG,Camacho NP

更新日期：2005-05-01 00:00:00

abstract：:We present an imaging-based method for noncontact spirometry. The method tracks the subtle respiratory-induced shoulder movement of a subject, builds a calibration curve, and determines the flow-volume spirometry curve and vital respiratory parameters, including forced expiratory volume in the first second, forced vit...

journal_title：Journal of biomedical optics

authors： Liu C,Yang Y,Tsow F,Shao D,Tao N

更新日期：2017-05-01 00:00:00

abstract：:A computer simulation called RHME (Retinal Heating in Moving Eye) is developed to simulate the heating pattern that occurs in the retina during a long-duration exposure to a continuous wave laser beam. The simulation takes into account eye movements that occur during a deliberate fixation. Due to the rapid (millisecon...

journal_title：Journal of biomedical optics

authors： Lund BJ

更新日期：2004-09-01 00:00:00

abstract：:A model that describes the concentration of photothermal (light-to-heat converters) compounds as a function of depth in a turbid medium is developed. The system consists of a pump laser (808 nm modulated at 400 Hz), which heats a photothermal compound, and a phase sensitive spectral domain optical coherence tomography...

journal_title：Journal of biomedical optics

authors： Guan G,Reif R,Huang Z,Wang RK

更新日期：2011-12-01 00:00:00

abstract：:When irradiated with nanosecond laser pulses, gold nanoparticles allow for manipulation or destruction of cells and proteins with high spatial and temporal precision. Gold nanorods are especially attractive, because they have an up-to-20-fold stronger absorption than a sphere of equal volume, which is shifted to the o...

journal_title：Journal of biomedical optics

authors： Rudnitzki F,Bever M,Rahmanzadeh R,Brieger K,Endl E,Groll J,Hüttmann G

更新日期：2012-05-01 00:00:00

abstract：:We present the first experimental result of direct delineation of the nuclei of living rat bladder epithelium with ultrahigh-resolution optical coherence tomography (uOCT). We demonstrate that the cellular details embedded in the speckle noise in a uOCT image can be uncovered by time-lapse frame averaging that takes a...

journal_title：Journal of biomedical optics

authors： Pan YT,Wu ZL,Yuan ZJ,Wang ZG,Du CW

更新日期：2007-09-01 00:00:00

abstract：:We fabricate scaffolds with a fine linear grating (periodicity of 1 to 8 microm and height of 1 microm) on a glass substrate for controlling cardiomyocyte orientation. The fabrication is done by the solidification of a liquid photopolymerizable material using two laser beam interference. As the photopolymerizable mate...

journal_title：Journal of biomedical optics

authors： Fujita A,Fujita K,Nakamura O,Matsuda T,Kawata S

更新日期：2006-03-01 00:00:00

abstract：:Many optical diagnostic approaches rely on changes in scattering and absorption properties to generate optical contrast between normal and diseased tissue. Recently, there has been increasing interest in using exogenous agents to enhance this intrinsic contrast with particular emphasis on the development for targeting...

journal_title：Journal of biomedical optics

authors： Lin AW,Lewinski NA,West JL,Halas NJ,Drezek RA

更新日期：2005-11-01 00:00:00

abstract：:A quantification method to measure endocytosis was designed to assess cellular uptake and specificity of a targeting nanoparticle platform. A simple N -hydroxysuccinimide ester conjugation technique to functionalize 100-nm hollow silica nanoshell particles with fluorescent reporter fluorescein isothiocyanate and folat...

journal_title：Journal of biomedical optics

authors： Sandoval S,Mendez N,Alfaro JG,Yang J,Aschemeyer S,Liberman A,Trogler WC,Kummel AC

更新日期：2015-08-01 00:00:00

abstract：:Raman spectroscopy is an optical technique able to interrogate biological tissues, giving us an understanding of the changes in molecular structure that are associated with disease development. The Kerr-gated Raman spectroscopy technique uses a picosecond pulsed laser as well as fast temporal gating of collected Raman...

journal_title：Journal of biomedical optics

authors： Prieto MC,Matousek P,Towrie M,Parker AW,Wright M,Ritchie AW,Stone N

更新日期：2005-07-01 00:00:00

abstract：:Diffusion of therapeutic macromolecules through the extracellular matrix of tumor tissue is a crucial step in drug delivery. We use fluorescence correlation spectroscopy (FCS) to measure diffusion of IgG (150 kDa) and dextrans (155 kDa and 2 MDa) in solution, 5% gelatin hydrogel, and multicellular spheroids. Gel and s...

journal_title：Journal of biomedical optics

authors： Reitan NK,Juthajan A,Lindmo T,de Lange Davies C

更新日期：2008-09-01 00:00:00

abstract：:Limited cutaneous systemic sclerosis (lcSSc) was formerly known as CREST syndrome in reference to the associated clinical features: calcinosis, Raynaud's phenomenon, esophageal dysfunction, sclerodactyly, and telangiectasias. The transforming growth factor beta has been identified as a major player in the pathogenic p...

journal_title：Journal of biomedical optics

authors： Barolet D

更新日期：2014-01-01 00:00:00

abstract：SIGNIFICANCE:The potential of fluorescence lifetime imaging microscopy (FLIM) is recently being recognized, especially in biological studies. However, FLIM does not directly measure the lifetimes, rather it records the fluorescence decay traces. The lifetimes and/or abundances have to be estimated from these traces dur...

journal_title：Journal of biomedical optics

authors： Guo S,Silge A,Bae H,Tolstik T,Meyer T,Matziolis G,Schmitt M,Popp J,Bocklitz T

更新日期：2021-01-01 00:00:00

abstract：:Dihydroartemisinin (DHA), a front-line antimalarial herbal compound, has been shown to possess promising anticancer activity with low toxicity. We have previously reported that DHA induced caspase-3-dependent apoptosis in human lung adenocarcinoma cells. However, the cellular target and molecular mechanism of DHA-indu...

journal_title：Journal of biomedical optics

authors： Lu YY,Chen TS,Wang XP,Li L

更新日期：2010-07-01 00:00:00

abstract：:Infrared microscopic imaging has been utilized to analyze for the first time the spatial distribution of lipid structure in an ex vivo human organ culture skin wound healing model. Infrared images were collected at zero, two, four, and six days following wounding. Analysis of lipid infrared spectral properties reveale...

journal_title：Journal of biomedical optics

authors： Yu G,Stojadinovic O,Tomic-Canic M,Flach CR,Mendelsohn R

更新日期：2012-09-01 00:00:00

abstract：:Waveguide evanescent field fluorescence (WEFF) microscopy is a new development that allows the imaging of contact regions between biological cells and their substratum, as well as imaging of ultrathin films such as monomolecular Langmuir-Blodgett (LB) films. Mixed-lipid monolayer systems mimicking lung surfactant were...

journal_title：Journal of biomedical optics

authors： Hassanzadeh A,Mittler S

更新日期：2011-04-01 00:00:00

abstract：:The objective is to characterize the impact of different ablation parameters on the thermal load during corneal refractive surgery by means of excimer laser ablation on porcine eyes. One hundred eleven ablations were performed in 105 porcine eyes. Each ablation was recorded using infrared thermography and analyzed mai...

journal_title：Journal of biomedical optics

authors： Arba Mosquera S,Verma S

更新日期：2015-07-01 00:00:00

abstract：:Fluorescence spectroscopy is a promising technology for detection of epithelial precancers and cancers. While age and menopausal status influence measurements in the cervix, other variables do not significantly affect the diagnosis. In this study we examine probe pressure as a variable. A fiber optic probe to measure ...

journal_title：Journal of biomedical optics

authors： Nath A,Rivoire K,Chang S,Cox D,Atkinson EN,Follen M,Richards-Kortum R

更新日期：2004-05-01 00:00:00

abstract：:Anastomotic complication is a major morbidity associated with esophagectomy. Gastric ischemia after conduit creation contributes to anastomotic complications, but a reliable method to assess oxygenation in the gastric conduit is lacking. We hypothesize that fiber optic spectroscopy can reliably assess conduit oxygenat...

journal_title：Journal of biomedical optics

authors： Gareau DS,Truffer F,Perry KA,Pham TH,Enestvedt CK,Dolan JP,Hunter JG,Jacques SL

更新日期：2010-11-01 00:00:00

abstract：:Inexpensive near-infrared microscopy (NIRM) was developed as a convenient technique to detect the medulla loss of scalp hair while reducing analytical time with easy sample preparation, leading to a field screening tool for breast cancer. NIRM has been evaluated as an alternative to synchrotron-based nanoscopy and to ...

journal_title：Journal of biomedical optics

authors： Choi Y,Jeong YJ,Jeon JG,Park SH,Choi HR,Kim JK

更新日期：2019-09-01 00:00:00

abstract：:Bacterial contamination of blood products is one of the most frequent infectious complications of transfusion. Since glucose levels in blood supplies decrease as bacteria proliferate, it should be possible to detect the presence of bacterial contamination by measuring the glucose concentrations in the blood components...

journal_title：Journal of biomedical optics

authors： Suzuki Y,Maruo K,Zhang AW,Shimogaki K,Ogawa H,Hirayama F

更新日期：2012-01-01 00:00:00

abstract：:The conical shape of the tympanic membrane (TM or eardrum) plays an important role in its function, such that variations in shape alter the acoustically induced motions of the TM. We present a method that precisely determines both shape and acoustically induced transient response of the entire TM using the same optics...

journal_title：Journal of biomedical optics

authors： Razavi P,Tang H,Rosowski JJ,Furlong C,Cheng JT

更新日期：2018-09-01 00:00:00

abstract：:We investigate the statistical polarization parameters of biological tissue histological section images with different morphological structure. First we outline the results of polarization coordinate mapping and analysis of the statistics of the first to fourth orders of biological tissue image polarization azimuth an...

journal_title：Journal of biomedical optics

authors： Angelsky OV,Ushenko AG,Ushenko YA,Ushenko YG,Tomka YY,Pishak VP

更新日期：2005-11-01 00:00:00

abstract：:Current techniques to assess lymph node metastases in cancer patients include lymphoscintigraphy after administration of a nonspecific radiocolloid in order to locate and resect lymph nodes for pathological examination of harbored cancer cells. Clinical trials involving intradermal or subcutaneous injection of antibod...

journal_title：Journal of biomedical optics

authors： Sampath L,Wang W,Sevick-Muraca EM

更新日期：2008-07-01 00:00:00