Abstract:
:We present a simple wide-field imaging technique, called HiLo microscopy, that is capable of producing optically sectioned images in real time, comparable in quality to confocal laser scanning microscopy. The technique is based on the fusion of two raw images, one acquired with speckle illumination and another with standard uniform illumination. The fusion can be numerically adjusted, using a single parameter, to produce optically sectioned images of varying thicknesses with the same raw data. Direct comparison between our HiLo microscope and a commercial confocal laser scanning microscope is made on the basis of sectioning strength and imaging performance. Specifically, we show that HiLo and confocal 3-D imaging of a GFP-labeled mouse brain hippocampus are comparable in quality. Moreover, HiLo microscopy is capable of faster, near video rate imaging over larger fields of view than attainable with standard confocal microscopes. The goal of this paper is to advertise the simplicity, robustness, and versatility of HiLo microscopy, which we highlight with in vivo imaging of common model organisms including planaria, C. elegans, and zebrafish.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Lim D,Ford TN,Chu KK,Mertz Jdoi
10.1117/1.3528656subject
Has Abstractpub_date
2011-01-01 00:00:00pages
016014issue
1eissn
1083-3668issn
1560-2281journal_volume
16pub_type
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journal_title:Journal of biomedical optics
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pub_type: 杂志文章
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