Abstract:
:Nanobioconjugates have been synthesized using cadmium selenide quantum dots (QDs), europium complexes (EuCs), and biotin. In those conjugates, long-lived photoluminescence (PL) is provided by the europium complexes, which efficiently transfer energy via Förster resonance energy transfer (FRET) to the QDs in close spatial proximity. As a result, the conjugates have a PL emission spectrum characteristic for QDs combined with the long PL decay time characteristic for EuCs. The nanobioconjugates synthesis strategy and photophysical properties are described as well as their performance in a time-resolved streptavidin-biotin PL assay. In order to prepare the QD-EuC-biotin conjugates, first an amphiphilic polymer has been functionalized with the EuC and biotin. Then, the polymer has been brought onto the surface of the QDs (either QD655 or QD705) to provide functionality and to make the QDs water dispersible. Due to a short distance between EuC and QD, an efficient FRET can be observed. Additionally, the QD-EuC-biotin conjugates’ functionality has been demonstratedin a PL assay yielding good signal discrimination, both from autofluorescence and directly excited QDs. These newly designed QD-EuC-biotin conjugates expand the class of highly sensitive tools for bioanalytical optical detection methods for diagnostic and imaging applications.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Cywinski PJ,Hammann T,Hühn D,Parak WJ,Hildebrandt N,Löhmannsröben HGdoi
10.1117/1.JBO.19.10.101506subject
Has Abstractpub_date
2014-01-01 00:00:00pages
101506issue
10eissn
1083-3668issn
1560-2281pii
1886851journal_volume
19pub_type
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