Fluorescence microscopy imaging of electroperturbation in mammalian cells.

abstract：:SpineLab is a software tool developed for reconstructing neuronal feature skeletons from three-dimensional single- or multi-photon image stacks. These images often suffer from limited resolution and a low signal-to-noise ratio, making the extraction of morphometric information difficult. To overcome this limitation, w...

journal_title：Journal of biomedical optics

authors： Jungblut D,Vlachos A,Schuldt G,Zahn N,Deller T,Wittum G

更新日期：2012-07-01 00:00:00

abstract：:Virus inactivation in red blood cell concentrates (RBCC) is being studied in order to increase the safety of the blood supply. For this purpose we have been studying the silicon phthalocyanine (Pc 4), a photosensitizer activated with red light. Two approaches were used to achieve enhanced selectivity of Pc 4 for virus...

journal_title：Journal of biomedical optics

authors： Ben-Hur E,Zuk MM,Oetjen J,Chan WS,Lenny L,Horowitz B

更新日期：1999-07-01 00:00:00

abstract：:We report that the pathologic components present within the atheromatous plaques of ApoE knock-out mice can reflect significant amounts of mid-infrared (mid-IR) light. Furthermore, the reflected light spectra contained the unique signatures of a variety of biologic features including those found in unstable or "vulner...

journal_title：Journal of biomedical optics

authors： Holman HY,Bjornstad KA,Martin MC,McKinney WR,Blakely EA,Blankenberg FG

更新日期：2008-05-01 00:00:00

abstract：:Pilot results on the application of the finite-difference time-domain (FDTD) approach for studying the implementation of the optical immersion technique for the visualization of single and multiple gold nanoparticles in biological cells are presented. We focus on two different scenarios considering single biological c...

journal_title：Journal of biomedical optics

authors： Tanev S,Tuchin VV,Paddon P

更新日期：2006-11-01 00:00:00

abstract：:In the present study, we evaluated the applicability of ex vivo photoacoustic imaging (PAI) on small animal organs. We used photoacoustic tomography (PAT) to visualize infarcted areas within murine hearts and compared these data to other imaging techniques [magnetic resonance imaging (MRI), micro-computed tomography] ...

journal_title：Journal of biomedical optics

authors： Holotta M,Grossauer H,Kremser C,Torbica P,Völkl J,Degenhart G,Esterhammer R,Nuster R,Paltauf G,Jaschke W

更新日期：2011-03-01 00:00:00

abstract：:Frequency-domain diffuse optical spectroscopy (FD-DOS) utilizes intensity-modulated light to characterize optical scattering and absorption in thick tissue. Previous FD-DOS systems have been limited by large device footprints, complex electronics, high costs, and limited acquisition speeds, all of which complicate acc...

journal_title：Journal of biomedical optics

authors： Torjesen A,Istfan R,Roblyer D

更新日期：2017-03-01 00:00:00

abstract：:Cancer-related enzyme activity can be detected noninvasively using activatable fluorescent molecular probes. In contrast to "always-on" fluorescent molecular probes, activatable probes are relatively nonfluorescent at the time of administration due to intramolecular fluorescence resonance energy transfer (FRET). Enzym...

journal_title：Journal of biomedical optics

authors： Solomon M,Guo K,Sudlow GP,Berezin MY,Edwards WB,Achilefu S,Akers WJ

更新日期：2011-06-01 00:00:00

abstract：:Diffuse optical spectroscopy (DOS) provides a powerful tool for fast and noninvasive disease diagnosis. The ability to leverage DOS to accurately quantify tissue optical parameters hinges on the model used to estimate light-tissue interaction. We describe the accuracy of a lookup table (LUT)-based inverse model for me...

journal_title：Journal of biomedical optics

authors： Nichols BS,Rajaram N,Tunnell JW

更新日期：2012-05-01 00:00:00

abstract：:We report an accurate optical differentiation technique between healthy and malaria-infected erythrocytes by quasi-simultaneous measurements of transmittance, reflectance, and scattering properties of unstained blood smears using a multispectral and multimode light-emitting diode microscope. We propose a technique for...

journal_title：Journal of biomedical optics

authors： Merdasa A,Brydegaard M,Svanberg S,Zoueu JT

更新日期：2013-03-01 00:00:00

abstract：:Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spont...

journal_title：Journal of biomedical optics

authors： Lee K,Kinnunen M,Khokhlova MD,Lyubin EV,Priezzhev AV,Meglinski I,Fedyanin AA

更新日期：2016-03-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) in tumor cells was found to play an important role in maintaining the malignant phenotype. Using laser scanning confocal fluorescence microscopy (LSCFM) in a recent work, we reported a variable fluorescence intensity of ethidium bromide (EB) in mitochondria nucleoids of living carcinoma cells...

journal_title：Journal of biomedical optics

authors： Villa AM,Fusi P,Pastori V,Amicarelli G,Pozzi C,Adlerstein D,Doglia SM

更新日期：2012-04-01 00:00:00

abstract：:Optical coherence tomography (OCT) is a noninvasive imaging methodology that is able to image tissue to depths of over 1 mm. Many epithelial conditions, such as melanoma and oral cancers, require an invasive biopsy for diagnosis. A noninvasive, real-time, point of care method of imaging depth-resolved epithelial struc...

journal_title：Journal of biomedical optics

authors： Smith LE,Hearnden V,Lu Z,Smallwood R,Hunter KD,Matcher SJ,Thornhill MH,Murdoch C,MacNeil S

更新日期：2011-11-01 00:00:00

abstract：:Cellularity of the fibrous caps of coronary atheromas, manifested by the infiltration of macrophages (average size, 20 to 30 microm), is thought to weaken the structural integrity of the cap and predispose plaques to rupture. Therefore, an imaging technology capable of identifying macrophages within fibroatheroma caps...

journal_title：Journal of biomedical optics

authors： Tanaka A,Tearney GJ,Bouma BE

更新日期：2010-01-01 00:00:00

abstract：:Fluorescence spectroscopy is a promising technology for detection of epithelial precancers and cancers. While age and menopausal status influence measurements in the cervix, other variables do not significantly affect the diagnosis. In this study we examine probe pressure as a variable. A fiber optic probe to measure ...

journal_title：Journal of biomedical optics

authors： Nath A,Rivoire K,Chang S,Cox D,Atkinson EN,Follen M,Richards-Kortum R

更新日期：2004-05-01 00:00:00

abstract：:This study aimed to evaluate the concept of using high-resolution optical coherence tomography (OCT) imaging to rapidly assess surgical specimens and determine if cancer positive margins were left behind in the surgical bed. A mouse model of breast cancer was used in this study. Surgical specimens from 30 animals were...

journal_title：Journal of biomedical optics

authors： Savastru D,Chang EW,Miclos S,Pitman MB,Patel A,Iftimia N

更新日期：2014-05-01 00:00:00

abstract：:The excited state lifetime of a fluorophore together with its fluorescence emission spectrum provide information that can yield valuable insights into the nature of a fluorophore and its microenvironment. However, it is difficult to obtain both channels of information in a conventional scheme as detectors are typicall...

journal_title：Journal of biomedical optics

authors： Sagar MAK,Dai B,Chacko J,Weber J,Velten A,Sanders S,White J,Eliceiri K

更新日期：2019-12-01 00:00:00

abstract：:A synchrotron light source dedicated to medical applications has been designed at National Institute of Radiological Sciences. The storage ring, with circumference of 80 m, is designed for acceleration of 2.3 GeV and a stored current of 420 mA. It is equipped with two multipole wigglers to produce sufficient photon fl...

journal_title：Journal of biomedical optics

authors： Torikoshi M,Tsunoo T,Endo M,Noda K,Kumada M,Yamada S,Soga F,Hyodo K

更新日期：2001-07-01 00:00:00

abstract：:We used two-photon laser scanning microscopy (TPLSM) to demonstrate for the first time its potential in studying relational details at the cellular level of atherogenesis in intact, viable mouse carotid arteries. Isolated and mounted arteries of ApoE-/-mice, aged 15 or 21 weeks (7 and 13 weeks on western diet), were i...

journal_title：Journal of biomedical optics

authors： Megens RT,oude Egbrink MG,Merkx M,Slaaf DW,van Zandvoort MA

更新日期：2008-07-01 00:00:00

abstract：:Optical coherence tomography (OCT) can provide new insight into disease progression and therapy by enabling nondestructive, serial imaging of in vivo cancer models. In previous studies, we have shown the utility of endoscopic OCT for identifying adenomas in the azoxymethane-treated mouse model of colorectal cancer and...

journal_title：Journal of biomedical optics

authors： Winkler AM,Rice PF,Drezek RA,Barton JK

更新日期：2010-07-01 00:00:00

abstract：:High-speed (video-rate) fluorescence lifetime imaging (FLIM) through a flexible endoscope is reported based on gated optical image intensifier technology. The optimization and potential application of FLIM to tissue autofluorescence for clinical applications are discussed. ...

journal_title：Journal of biomedical optics

authors： Munro I,McGinty J,Galletly N,Requejo-Isidro J,Lanigan PM,Elson DS,Dunsby C,Neil MA,Lever MJ,Stamp GW,French PM

更新日期：2005-09-01 00:00:00

abstract：:Polarized Raman spectroscopy allows measurement of molecular orientation and composition and is widely used in the study of polymer systems. Here, we extend the technique to the extraction of quantitative orientation information from bone tissue, which is optically thick and highly turbid. We discuss multiple scatteri...

journal_title：Journal of biomedical optics

authors： Raghavan M,Sahar ND,Wilson RH,Mycek MA,Pleshko N,Kohn DH,Morris MD

更新日期：2010-05-01 00:00:00

abstract：:Amblyopia is a form of visual impairment caused by ocular misalignment (strabismus) or defocus in an otherwise healthy eye. If detected early, the condition can be fully treated, yet over half of all children with amblyopia under age 5 escape detection. We developed a Pediatric Vision Screener (PVS) to detect amblyopi...

journal_title：Journal of biomedical optics

authors： Nassif DS,Piskun NV,Gramatikov BI,Guyton DL,Hunter DG

更新日期：2004-11-01 00:00:00

abstract：:A multifunctional line scanning ophthalmoscope (mLSO) was designed, constructed, and tested on human subjects. The mLSO could sequentially acquire wide-field, confocal, near-infrared reflectance, fluorescein angiography (FA), and indocyanine green angiography (ICGA) retinal images. The system also included a retinal t...

journal_title：Journal of biomedical optics

authors： Hammer DX,Ferguson RD,Patel AH,Vazquez V,Husain D

更新日期：2012-02-01 00:00:00

abstract：:Traditional phase-resolved Doppler optical coherence tomography (DOCT) has been reported to have potential for characterizing local fluid flow within a microporous scaffold. In this work, we apply Doppler optical microangiography (DOMAG), a new imaging technique developed by combining optical microangiography (OMAG) w...

journal_title：Journal of biomedical optics

authors： Jia Y,An L,Wang RK

更新日期：2009-09-01 00:00:00

abstract：:In order to evaluate the impact of anatomy on the spectral properties of oral tissue, we used reflectance and fluorescence spectroscopy to characterize nine different anatomic sites. All spectra were collected in vivo from healthy oral mucosa. We analyzed 710 spectra collected from the oral cavity of 79 healthy volunt...

journal_title：Journal of biomedical optics

authors： McGee S,Mirkovic J,Mardirossian V,Elackattu A,Yu CC,Kabani S,Gallagher G,Pistey R,Galindo L,Badizadegan K,Wang Z,Dasari R,Feld MS,Grillone G

更新日期：2008-11-01 00:00:00

abstract：:The scattering of polarized light from a two layer scattering medium is investigated using Monte Carlo simulations. First order and normalized second order moments are used to analyze the spatial properties of the emerging light in different polarization states. Linearly and circularly polarized illumination is used t...

journal_title：Journal of biomedical optics

authors： Stockford IM,Morgan SP,Chang PC,Walker JG

更新日期：2002-07-01 00:00:00

abstract：:Application of a fiber optic biosensor (FOB) to the real-time investigation of the interaction kinetics between FITC-conjugated monoclonal sheep anti-human C-reactive protein (CRP) antibody and CRP isoforms on the surface of optical fiber is described. Recently, both the native pentameric CRP (pCRP), an acute phase pr...

journal_title：Journal of biomedical optics

authors： Chou C,Hsu HY,Wu HT,Tseng KY,Chiou A,Yu CJ,Lee ZY,Chan TS

更新日期：2007-03-01 00:00:00

abstract：:Diffusion of two model drugs-benzyl nicotinate and ibuprofen-and the plasma macromolecule albumin across atherosclerotic rabbit aorta was studied ex vivo by attenuated total reflection-Fourier transform infrared (ATR-FTIR) imaging. Solutions of these molecules were applied to the endothelial surface of histological se...

journal_title：Journal of biomedical optics

authors： Palombo F,Danoux CB,Weinberg PD,Kazarian SG

更新日期：2009-07-01 00:00:00

abstract：:The expensive fabrication of current opto-microfluidic sensors is a barrier to the successful adoption of these devices in point-of-care testing. This work reports a simple inexpensive opto-microfluidic device incorporating a poly(dimethylsiloxane)-glass hybrid microfluidic chip modified with gold nanoparticles and a ...

journal_title：Journal of biomedical optics

authors： Pires NM,Dong T

更新日期：2014-03-01 00:00:00

abstract：:Recent technological advancements in the photonics industry have led to a resurgence of interest in optical glucose sensing and to realistic progress toward the development of an optical glucose sensor. Such a sensor has the potential to significantly improve the quality of life for the estimated 16 million diabetics ...

journal_title：Journal of biomedical optics

authors： McNichols RJ,Coté GL

更新日期：2000-01-01 00:00:00