Two-photon microscopy on vital carotid arteries: imaging the relationship between collagen and inflammatory cells in atherosclerotic plaques.

abstract：:We demonstrate a quasinoncontact photoacoustic imaging method using a homodyne interferometer with a long coherence length laser. The generated photoacoustic signal is detected by a system that is locked at its maximum sensitivity through the use of balanced detection and zero-crossing triggering. The balanced detecto...

journal_title：Journal of biomedical optics

authors： Lu J,Gao Y,Ma Z,Zhou H,Wang RK,Wang Y

更新日期：2017-03-01 00:00:00

abstract：:Fluorescence imaging for detection of non-muscle-invasive bladder cancer is based on the selective production and accumulation of fluorescing porphyrins-mainly, protoporphyrin IX-in cancerous tissues after the instillation of Hexvix®. Although the sensitivity of this procedure is very good, its specificity is somewhat...

journal_title：Journal of biomedical optics

authors： Lovisa B,Jichlinski P,Weber BC,Aymon D,van den Bergh H,Wagnières G

更新日期：2010-09-01 00:00:00

abstract：:Intercellular heterogeneity is a key factor in a variety of core cellular processes including proliferation, stimulus response, carcinogenesis, and drug resistance. However, cell-to-cell variability studies at the single-cell level have been hampered by the lack of enabling experimental techniques. We present a measur...

journal_title：Journal of biomedical optics

authors： Kelbauskas L,Ashili SP,Houkal J,Smith D,Mohammadreza A,Lee KB,Forrester J,Kumar A,Anis YH,Paulson TG,Youngbull CA,Tian Y,Holl MR,Johnson RH,Meldrum DR

更新日期：2012-03-01 00:00:00

abstract：:We present three-dimensional (3-D) in vivo photoacoustic (PA) images of the blood vasculature of a chicken chorioallantoic membrane (CAM) obtained by using a fiber-based noncontact PA tomography system. With a fiber-optic heterodyne interferometer, the system measures the surface displacement of a sample, induced by t...

journal_title：Journal of biomedical optics

authors： Eom J,Park SJ,Lee BH

更新日期：2015-10-01 00:00:00

abstract：:A promising method to achieve rapid convergence for image reconstruction is introduced for the continuous-wave near-infrared (NIR) diffuse optical tomography (DOT). Tomographic techniques are usually implemented off line and are time consuming to realize image reconstruction, especially for NIR DOT. Therefore, it is e...

journal_title：Journal of biomedical optics

authors： Pan MC,Pan MC

更新日期：2010-01-01 00:00:00

abstract：:This paper presents an effective two-step scheme for automatic object detection in computed radiography (CR) images. First, various structure elements of the morphological filters, designed by incorporating available morphological features of the objects of interest including their sizes and rough shape descriptions, ...

journal_title：Journal of biomedical optics

authors： Xuan J,Adali T,Wang Y,Siegel E

更新日期：2000-10-01 00:00:00

abstract：:Autofluorescence of the cornea within specific wavelength region and dynamic light scattering measurements of ocular tissue have both been used for early-stage detection of the presence of diabetic retinopathy. In the present study, autofluorescence of the cornea and dynamic light scattering in the cornea and vitreous...

journal_title：Journal of biomedical optics

authors： Rovati L,Fankhauser F,Docchio F,Van Best J

更新日期：1998-07-01 00:00:00

abstract：:Quantitative spectroscopy has recently been extended from a contact-probe to wide-area spectroscopic imaging to enable mapping of optical properties across a wide area of tissue. We train quantitative spectroscopic imaging (QSI) to identify cervical high-grade squamous intraepithelial lesions (HSILs) in 34 subjects un...

journal_title：Journal of biomedical optics

authors： Lau C,Mirkovic J,Yu CC,O'Donoghue GP,Galindo L,Dasari R,de las Morenas A,Feld M,Stier E

更新日期：2013-07-01 00:00:00

abstract：:The metabolic rate of oxygen consumption, an important indicator of tissue metabolism, can be expressed as the change of net blood oxygen flux into and out of a tissue region per 100 g of tissue. In this work, we propose a photoacoustic and Doppler ultrasound method for imaging local blood oxygen flux of a single vess...

journal_title：Journal of biomedical optics

authors： Jiang Y,Forbrich A,Harrison T,Zemp RJ

更新日期：2012-03-01 00:00:00

abstract：:Ex vivo human skin has been used extensively for cosmeceutical and drug delivery studies, transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to ischemic necrosis. Traditional methods of assessing viability can be time-consuming and provide limited metabolic information. Using ...

journal_title：Journal of biomedical optics

authors： Sanchez WY,Prow TW,Sanchez WH,Grice JE,Roberts MS

更新日期：2010-07-01 00:00:00

abstract：:Reactive oxygen species (ROS) produced by photodynamic therapy (PDT) is recorded in vivo using a chemiluminescence (CL)-based gated optical system. A novel approach is developed to utilize the fluorescence (FL) of the CL probe as an internal fluorescence to calibrate the observed CL on pharmacokinetics of the probe in...

journal_title：Journal of biomedical optics

authors： Wei Y,Xing D,Luo S,Yang L,Chen Q

更新日期：2010-03-01 00:00:00

abstract：:Collagen fibers can be imaged with second harmonic generation (SHG) and are associated with efficient tumor cell locomotion. Preferential locomotion along these fibers correlates with a more aggressively metastatic phenotype, and changes in SHG emission properties accompany changes in metastatic outcome. We therefore ...

journal_title：Journal of biomedical optics

authors： Burke RM,Madden KS,Perry SW,Zettel ML,Brown EB 3rd

更新日期：2013-08-01 00:00:00

abstract：:Myocardial infarction leads to structural remodeling of the myocardium, in particular to the loss of cardiomyocytes due to necrosis and an increase in collagen with scar formation. Stem cell regenerative treatments have been shown to alter this remodeling process, resulting in improved cardiac function. As healthy myo...

journal_title：Journal of biomedical optics

authors： Wood MF,Ghosh N,Wallenburg MA,Li SH,Weisel RD,Wilson BC,Li RK,Vitkin IA

更新日期：2010-07-01 00:00:00

abstract：:We have developed a novel parallel-plate diffuse optical tomography (DOT) system for three-dimensional in vivo imaging of human breast tumor based on large optical data sets. Images of oxy-, deoxy-, and total hemoglobin concentration as well as blood oxygen saturation and tissue scattering were reconstructed. Tumor ma...

journal_title：Journal of biomedical optics

authors： Choe R,Konecky SD,Corlu A,Lee K,Durduran T,Busch DR,Pathak S,Czerniecki BJ,Tchou J,Fraker DL,Demichele A,Chance B,Arridge SR,Schweiger M,Culver JP,Schnall MD,Putt ME,Rosen MA,Yodh AG

更新日期：2009-03-01 00:00:00

abstract：:Intraoperative fluorescence molecular imaging based on targeted fluorescence agents is an emerging approach to improve surgical and endoscopic imaging and guidance. Short exposure times per frame and implementation at video rates are necessary to provide continuous feedback to the physician and avoid motion artifacts....

journal_title：Journal of biomedical optics

authors： Koch M,Glatz J,Ermolayev V,de Vries EG,van Dam GM,Englmeier KH,Ntziachristos V

更新日期：2014-04-01 00:00:00

abstract：:Resin embedding is widely used and facilitates microscopic imaging of biological tissues. In contrast, quenching of fluorescence during embedding process hinders the application of resin embedding for imaging of fluorescence-labeled samples. For samples expressing fluorescent proteins, it has been demonstrated that th...

journal_title：Journal of biomedical optics

authors： Li L,Rao G,Lv X,Chen R,Cheng X,Wang X,Zeng S,Liu X

更新日期：2018-02-01 00:00:00

abstract：:We develop a label-free optical technique to image and discriminate undifferentiated human embryonic stem cells (hESCs) from their differentiating progenies in vitro. Using intrinsic cellular fluorophores, we perform fluorescence lifetime microscopy (FLIM) and phasor analysis to obtain hESC metabolic signatures. We id...

journal_title：Journal of biomedical optics

authors： Stringari C,Sierra R,Donovan PJ,Gratton E

更新日期：2012-04-01 00:00:00

abstract：:Optical-resolution photoacoustic microscopy (ORPAM) in theory provides lateral resolution equivalent to the optical diffraction limit. Scattering media, such as biological turbid media, attenuates the optical signal and also alters the diffraction-limited spot size of the focused beam. The ORPAM signal is generated on...

journal_title：Journal of biomedical optics

authors： U-Thainual P,Kim DH

更新日期：2015-12-01 00:00:00

abstract：:We experimentally compare the performance and image contrast of spontaneous Raman and coherent Stokes Raman scattering microscopy. We demonstrate the differences between these techniques on a series of geometry-controlled samples that range in complexity from a point (array of tips) to one-dimensional (line grating) a...

journal_title：Journal of biomedical optics

authors： Kim H,Michaels CA,Bryant GW,Stranick SJ

更新日期：2011-02-01 00:00:00

abstract：:This paper presents a fiber Bragg grating (FBG)-instrumented prosthetic silicone liner that provides cushioning for the residual limb and can successfully measure interface pressures inside prosthetic sockets of lower-limb amputees in a simple and practical means of sensing. The liner is made of two silicone layers be...

journal_title：Journal of biomedical optics

authors： Al-Fakih E,Arifin N,Pirouzi G,Mahamd Adikan FR,Shasmin HN,Abu Osman NA

更新日期：2017-08-01 00:00:00

abstract：SIGNIFICANCE:The blood-brain barrier (BBB) is a major obstacle to detecting and treating brain tumors. Overcoming this challenge will facilitate the early and accurate detection of brain lesions and guide surgical resection of tumors. AIM:We generated an orthotopic brain tumor model that simulates the pathophysiology ...

journal_title：Journal of biomedical optics

authors： Habimana-Griffin L,Ye D,Carpenter J,Prior J,Sudlow G,Marsala L,Mixdorf M,Rubin J,Chen H,Achilefu S

更新日期：2020-02-01 00:00:00

abstract：:Alcohol testing is an expanding area of interest due to the impacts of alcohol abuse that extend well beyond drunk driving. However, existing approaches such as blood and urine assays are hampered in some testing environments by biohazard risks. A noninvasive, in vivo spectroscopic technique offers a promising alterna...

journal_title：Journal of biomedical optics

authors： Ridder TD,Ver Steeg BJ,Laaksonen BD

更新日期：2009-09-01 00:00:00

abstract：:A photoacoustic imaging system that incorporates a commercial ultrasonic camera for real-time imaging of two-dimensional (2-D) projection planes in tissue at video rate (30 Hz) is presented. The system uses a Q-switched frequency-doubled Nd:YAG pulsed laser for photoacoustic generation. The ultrasonic camera consists ...

journal_title：Journal of biomedical optics

authors： Balogun O,Regez B,Zhang HF,Krishnaswamy S

更新日期：2010-03-01 00:00:00

abstract：:Measuring the elasticity distribution inside the human body is of great interest because elastic abnormalities can serve as indicators of several diseases. We present a method for mapping elasticity inside soft tissues by imaging surface acoustic waves (SAWs) with digital holographic interferometry. With this method, ...

journal_title：Journal of biomedical optics

authors： Li S,Mohan KD,Sanders WW,Oldenburg AL

更新日期：2011-11-01 00:00:00

abstract：:Anastomotic complication is a major morbidity associated with esophagectomy. Gastric ischemia after conduit creation contributes to anastomotic complications, but a reliable method to assess oxygenation in the gastric conduit is lacking. We hypothesize that fiber optic spectroscopy can reliably assess conduit oxygenat...

journal_title：Journal of biomedical optics

authors： Gareau DS,Truffer F,Perry KA,Pham TH,Enestvedt CK,Dolan JP,Hunter JG,Jacques SL

更新日期：2010-11-01 00:00:00

abstract：:We demonstrate the first application of Raman spectroscopy in diagnosing nonmalignant, premalignant, malignant, and metastatic stages of breast cancer in a three-dimensional (3-D) cell culture model that closely mimics an in vivo environment. Comprehensive study comparing classification in two-dimensional (2-D) and 3-...

journal_title：Journal of biomedical optics

authors： Damayanti NP,Fang Y,Parikh MR,Craig AP,Kirshner J,Irudayaraj J

更新日期：2013-11-01 00:00:00

abstract：:Ultrasmall scale implants have recently attracted focus as valuable tools for monitoring both acute and chronic diseases. Semiconductor optical technologies are the key to miniaturizing these devices to the long-sought sub-mm scale, which will enable long-term use of these devices for medical applications. This can al...

journal_title：Journal of biomedical optics

authors： Mujeeb-U-Rahman M,Adalian D,Chang CF,Scherer A

更新日期：2015-09-01 00:00:00

abstract：:The effect of sampling region size and tissue heterogeneity is examined using fluorescence histogram assessment in a rat prostate tumor model with benzoporphyrin derivative fluorophore. Spatial heterogeneity in the fluorescence signal occurs on both macroscopic and microscopic scales. The periphery of the tumor is mor...

journal_title：Journal of biomedical optics

authors： Pogue BW,Chen B,Zhou X,Hoopes PJ

更新日期：2005-07-01 00:00:00

abstract：:Nanobioconjugates have been synthesized using cadmium selenide quantum dots (QDs), europium complexes (EuCs), and biotin. In those conjugates, long-lived photoluminescence (PL) is provided by the europium complexes, which efficiently transfer energy via Förster resonance energy transfer (FRET) to the QDs in close spat...

journal_title：Journal of biomedical optics

authors： Cywinski PJ,Hammann T,Hühn D,Parak WJ,Hildebrandt N,Löhmannsröben HG

更新日期：2014-01-01 00:00:00

abstract：:Hemoglobin (Hb), as one of main components of blood, has a unique quaternary structure. Its release of oxygen is controlled by oxygen partial pressure (PO2). We investigate the specific spectroscopic changes in Hb under different PO2 levels to optimize clinical methods of measuring tissue PO2. The transmissivity of Hb...

journal_title：Journal of biomedical optics

authors： Liu P,Zhu Z,Zeng C,Nie G

更新日期：2012-12-01 00:00:00