Abstract:
:The excited state lifetime of a fluorophore together with its fluorescence emission spectrum provide information that can yield valuable insights into the nature of a fluorophore and its microenvironment. However, it is difficult to obtain both channels of information in a conventional scheme as detectors are typically configured either for spectral or lifetime detection. We present a fiber-based method to obtain spectral information from a multiphoton fluorescence lifetime imaging (FLIM) system. This is made possible using the time delay introduced in the fluorescence emission path by a dispersive optical fiber coupled to a detector operating in time-correlated single-photon counting mode. This add-on spectral implementation requires only a few simple modifications to any existing FLIM system and is considerably more cost-efficient compared to currently available spectral detectors.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Sagar MAK,Dai B,Chacko J,Weber J,Velten A,Sanders S,White J,Eliceiri Kdoi
10.1117/1.JBO.25.1.014506subject
Has Abstractpub_date
2019-12-01 00:00:00pages
1-17issue
1eissn
1083-3668issn
1560-2281pii
JBO-190320SSRRjournal_volume
25pub_type
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