Abstract：
:Low-level light therapy has been shown to improve in vitro wound healing. However, well-defined parameters of different light sources for this therapy are lacking. The goal of this study was (1) to determine if the wavelengths tested are effective for in vitro wound healing and (2) to compare a laser and a light-emitting diode (LED) source at similar wavelengths. We show four wavelengths, delivered by either a laser or LED array, improved in vitro wound healing in A549, U2OS, and PtK2 cells. Improved wound healing occurred through increased cell migration demonstrated through scratch wound and transwell assays. Cell proliferation was tested by the (3-(4,5-dimethylthiazol-2-yl)-5-(3-car-boxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay and was found generally not to be involved in the wound healing process. The laser and LED sources were found to be comparable when equal doses of light were applied. The biological response measured was similar in most cases. We conclude that the laser at 652 (5.57 mW/cm2, 10.02 J/cm2) and 806 nm (1.30 mW/cm2, 2.334 J/cm2) (full bandwidth 5 nm), and LED at 637 (5.57 mW/cm2, 10.02 J/cm2) and 901 nm (1.30 mW/cm2, 2.334 J/cm2) (full bandwidth 17 and 69 nm respectively) induce comparable levels of cell migration and wound closure.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Spitler R,Berns MWdoi
10.1117/1.JBO.19.3.038001subject
Has Abstractpub_date
2014-03-01 00:00:00pages
38001issue
3eissn
1083-3668issn
1560-2281pii
1850277journal_volume
19pub_type
杂志文章abstract：:Reduction of optical scattering in turbid biological tissues using nonreactive chemical agents has potential applications for light-based diagnostics and therapeutics. Optical clearing effects by exogenous chemical agents, in particular sugars and sugar alcohols, have been found to be temporary with tissue rehydration...
journal_title：Journal of biomedical optics
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