Abstract:
:The morphogenetic relationship between early patterning and polarity formation is of fundamental interest and remains a controversial issue in preimplantation embryonic development. We use a label-free three-dimensional (3-D) imaging technique of full-field optical coherence tomography (FF-OCT) successfully for the first time to study the dynamics of developmental processes in mouse preimplantation lives. Label-free 3-D subcellular time-lapse images are demonstrated to investigate 3-D spatial relationship between the second polar body (2PB) and the first cleavage plane. By using FF-OCT together with quantitative study, we show that only 25% of the predicted first cleavage planes, defined by the apposing plane of two pronuclei, pass through the 2PB. Also only 27% of the real cleavage planes pass through the 2PB. These results suggest that the 2PB is not a convincing spatial cue for the event of the first cleavage. Our studies demonstrate the feasibility of FF-OCT in providing new insights and potential breakthroughs to the controversial issues of early patterning and polarity in mammalian developmental biology.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Zheng JG,Huo T,Chen T,Wang C,Zhang N,Tian N,Zhao F,Lu D,Chen D,Ma W,Sun JL,Xue Pdoi
10.1117/1.JBO.18.1.010503subject
Has Abstractpub_date
2013-01-01 00:00:00pages
10503issue
1eissn
1083-3668issn
1560-2281pii
1485890journal_volume
18pub_type
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journal_title:Journal of biomedical optics
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