Abstract:
:The intracellular refractive index is an important parameter that describes the optical density of the cytoplasm and the concentration of the intracellular solutes. The refractive index of adherently grown cells is difficult to access. We present a method in which silica microspheres in living cells are used to determine the cytoplasm refractive index with quantitative phase microscopy. The reliability of our approach for refractive index retrieval is shown by data from a comparative study on osmotically stimulated adherent and suspended human pancreatic tumor cells. Results from adherent human fibro sarcoma cells demonstrate the capability of the method for sensing of dynamic refractive index changes and its usage with microfluidics.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Przibilla S,Dartmann S,Vollmer A,Ketelhut S,Greve B,von Bally G,Kemper Bdoi
10.1117/1.JBO.17.9.097001subject
Has Abstractpub_date
2012-09-01 00:00:00pages
97001-1issue
9eissn
1083-3668issn
1560-2281pii
1357024journal_volume
17pub_type
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