Abstract:
:We present a novel confocal microscope that has dual-axis architecture and biaxial postobjective scanning for the collection of fluorescence images from biological specimens. This design uses two low-numerical-aperture lenses to achieve high axial resolution and long working distance, and the scanning mirror located distal to the lenses rotates along the orthogonal axes to produce arc-surface images over a large field of view (FOV). With fiber optic coupling, this microscope can potentially be scaled down to millimeter dimensions via microelectromechanical systems (MEMS) technology. We demonstrate a benchtop prototype with a spatial resolution < or =4.4 microm that collects fluorescence images with a high SNR and a good contrast ratio from specimens expressing GFP. Furthermore, the scanning mechanism produces only small differences in aberrations over the image FOV. These results demonstrate proof of concept of the dual-axis confocal architecture for in vivo molecular and cellular imaging.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Wang TD,Contag CH,Mandella MJ,Chan NY,Kino GSdoi
10.1117/1.1760760subject
Has Abstractpub_date
2004-07-01 00:00:00pages
735-42issue
4eissn
1083-3668issn
1560-2281journal_volume
9pub_type
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