Abstract:
:Photobiological research in the last 30 yr has shown the existence of ultraweak photon emission in biological tissue, which can be detected with sophisticated photomultiplier systems. Although the emission of this ultraweak radiation, often termed biophotons, is extremely low in mammalian cells, it can be efficiently increased by ultraviolet light. Most recently it was shown that UV-A (330 to 380 nm) releases such very weak cell radiation in differentiated human skin fibroblasts. Based on these findings, a new and powerful tool in the form of UV-A-laser-induced biophotonic emission of cultured cells was developed with the intention to detect biophysical changes between carcinogenic and normal cells. With suspension densities ranging from 1 to 8 x 10(6) cells/mL, it was evident that an increase of the UV-A-laser-light induced photon emission intensity could be observed in normal as well as melanoma cells. Using this new detection procedure of ultraweak light emission, photons in cell suspensions as low as 100 microL could be determined, which is a factor of 100 lower compared to previous procedures. Moreover, the detection procedure has been further refined by turning off the photomultiplier system electronically during irradiation leading to the first measurements of induced light emission in the cells after less than 10 micros instead of 150 ms, as reported in previous procedures. This improvement leads to measurements of light bursts up 10(7) photons/s instead of several hundred as found with classical designs. Overall, we find decreasing induction ratings between normal and melanoma cells as well as cancer-prone and melanoma cells. Therefore, it turns out that this highly sensitive and noninvasive device enables us to detect high levels of ultraweak photon emission following UV-A-laser-induced light stimulation within the cells, which enables future development of new biophysical strategies in cell research.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Niggli HJ,Tudisco S,Privitera G,Applegate LA,Scordino A,Musumeci Fdoi
10.1117/1.1899185subject
Has Abstractpub_date
2005-03-01 00:00:00pages
024006issue
2eissn
1083-3668issn
1560-2281journal_volume
10pub_type
杂志文章abstract::A swept source (SS)-based circular-state (CS) polarization-sensitive optical coherence tomography (PS-OCT) constructed entirely with polarization-maintaining fiber optics components is proposed with the experimental verification. By means of the proposed calibration scheme, bulk quarter-wave plates can be replaced by ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.2.021110
更新日期:2014-02-01 00:00:00
abstract::Photoacoustic imaging has become an emerging tool for theranostic applications. Not only does it help in journal_title:Journal of biomedical optics pub_type: 杂志文章 doi:10.1117/1.JBO.22.4.041007 更新日期:2017-04-01 00:00:00
abstract::Laser-induced breakdown spectroscopy (LIBS) is applied to analyze human fingernails using nanosecond laser pulses. Measurements on 45 nail samples are carried out and 14 key species are identified. The elements detected with the present system are: Al, C, Ca, Fe, H, K, Mg, N, Na, O, Si, Sr, Ti as well as CN molecule. ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3574757
更新日期:2011-05-01 00:00:00
abstract:SIGNIFICANCE:In multiphoton microscopy, two-photon excited fluorescence (TPEF) spectra carry valuable information on morphological and functional biological features. For measuring these biomarkers, separation of different parts of the fluorescence spectrum into channels is typically achieved by the use of optical band...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.7.071206
更新日期:2020-07-01 00:00:00
abstract::We present a study using plasmonic nanoparticles (NPs) to image epidermal growth factor receptor (EGFR) in live cells. Through detailed analysis of the NP scattering spectra, we determine the intracellular refractive index (RI) within attoliter volumes inside of the living cells. Molecular imaging is demonstrated usin...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2837450
更新日期:2008-01-01 00:00:00
abstract::Using single-molecule microscopy, we present a method to quantify the number of single autofluorescent proteins when they cannot be optically resolved. This method relies on the measurement of the total intensity emitted by each aggregate until it photobleaches. This strategy overcomes the inherent problem of blinking...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2940600
更新日期:2008-05-01 00:00:00
abstract::Protoporphyrin IX (PpIX)-encapsulated mesoporous silica nanoparticles were synthesized, characterized, and utilized for photodynamic therapy (PDT) of cancer. Silica encapsulation is relatively transparent for activated light and can protect the PpIX against denaturation induced by the extreme bioenvironment. The mesop...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3083427
更新日期:2009-01-01 00:00:00
abstract::We propose a handheld projective imaging device for orthotopic projection of near-infrared fluorescence images onto target biological tissue at visible wavelengths without any additional visual aid. The device integrates a laser diode light source module, a camera module, a projector, an ultrasonic distance sensor, a ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.8.080503
更新日期:2019-08-01 00:00:00
abstract::The capabilities of micro-Raman spectroscopy for differentiating normal and malignant nasopharyngeal tissues were evaluated. Raman scattering signals were acquired from 22 normal and 52 malignant nasopharyngeal tissue samples. Distinctive spectral differences in Raman spectra between normal and malignant nasopharyngea...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.2.027003
更新日期:2013-02-01 00:00:00
abstract::The study of red blood cell (RBC) aggregation is of great interest because of its implications for human health. Altered RBC aggregation can lead to microcirculatory problems as in vascular pathologies, such as hypertension and diabetes, due to a decrease in the erythrocyte surface electric charge and an increase in t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.1.017003
更新日期:2017-01-01 00:00:00
abstract::Subsurface fluorescence molecular tomography (FMT) has promising potential for noninvasive characterization of molecular and cellular activities in small animals by tomographic means in reflectance geometry. In this work, subsurface FMT is employed to monitor the therapeutic response of cisplatin in tumor-bearing mice...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.JBO.17.4.040504
更新日期:2012-04-01 00:00:00
abstract::Fluorescence spectroscopy is a promising technology for detection of epithelial precancers and cancers. While age and menopausal status influence measurements in the cervix, other variables do not significantly affect the diagnosis. In this study we examine probe pressure as a variable. A fiber optic probe to measure ...
journal_title:Journal of biomedical optics
pub_type: 临床试验,杂志文章
doi:10.1117/1.1695562
更新日期:2004-05-01 00:00:00
abstract::Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1577574
更新日期:2003-07-01 00:00:00
abstract::We report a new two-channel fluorescence microscopy technique for surface-generated fluorescence. The realized fluorescence microscope allows high resolution imaging of aqueous samples. The core element of the instrument is a parabolic mirror objective that is used to collect the fluorescence at large surface angles a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2747627
更新日期:2007-05-01 00:00:00
abstract::Oxygen plays a very important role in living cells. The intracellular level of oxygen is under tight control, as even a small deviation from normal oxygen level affects major cellular metabolic processes and is likely to result in cellular damage or cell death. This paper describes the use of the oxygen sensitive fluo...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1344190
更新日期:2001-04-01 00:00:00
abstract::The use of reduced nicotinamide adenine dinucleotide (NADH) fluorescence to gain metabolic information on kidneys in response to an alteration in oxygen availability has previously been experimentally demonstrated, but signal quantification has not, to date, been addressed. In this work the relative contribution to ra...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3094948
更新日期:2009-03-01 00:00:00
abstract::We present three-dimensional (3-D) high-resolution spectral-domain optical coherence microscopy (SD-OCM) by using a supercontinuum (SC) fiber laser source with 300-nm spectral bandwidth (full-width at half-maximum) in the 1700-nm spectral band. By using low-coherence interferometry with SC light and a confocal detecti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.7.070502
更新日期:2019-07-01 00:00:00
abstract::Stenosis and occlusion of the superficial femoral artery (SFA) are most common in arterial occlusive disease. There are numerous interventional, surgical, and combined approaches to reconstitute maximum blood supply to the lower limb; however, despite intense clinical research, the long-term success rates are still po...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.2.025005
更新日期:2015-02-01 00:00:00
abstract::We present a hybrid method that combines a multilayered scaling method and a perturbation method to speed up the Monte Carlo simulation of diffuse reflectance from a multilayered tissue model with finite-size tumor-like heterogeneities. The proposed method consists of two steps. In the first step, a set of photon traj...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.1.010501
更新日期:2012-01-01 00:00:00
abstract::Our long-term goal was to investigate the potential of incorporating redox imaging technique as a breast cancer (BC) diagnosis component to increase the positive predictive value of suspicious imaging finding and to reduce unnecessary biopsies and overdiagnosis. We previously found that precancer and cancer tissues in...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.11.114003
更新日期:2016-11-01 00:00:00
abstract::Pulse oximeters are widely used for noninvasive monitoring of oxygen saturation in arterial blood hemoglobin. We present a transmittance pulse oximetry system based on near-infrared (NIR) laser diodes (750 and 850 nm) for monitoring oxygen saturation of arterial blood hemoglobin. The pulse oximetry system is made up o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1578495
更新日期:2003-07-01 00:00:00
abstract::Spatially resolved diffuse reflectance spectroscopy (srDRS) is a well-established technique for noninvasive, in vivo characterization of tissue optical properties toward diagnostic applications. srDRS has a potential for depth-resolved analysis of tissue, which is desired in various clinical situations. However, curre...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.11.115003
更新日期:2018-11-01 00:00:00
abstract::We have applied wide-field digital interferometry (WFDI) to examine the morphology and dynamics of live red blood cells (RBCs) from individuals who suffer from sickle cell anemia (SCA), a genetic disorder that affects the structure and mechanical properties of RBCs. WFDI is a noncontact, label-free optical microscopy ...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3556717
更新日期:2011-03-01 00:00:00
abstract::Autofluorescence of the cornea within specific wavelength region and dynamic light scattering measurements of ocular tissue have both been used for early-stage detection of the presence of diabetic retinopathy. In the present study, autofluorescence of the cornea and dynamic light scattering in the cornea and vitreous...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429882
更新日期:1998-07-01 00:00:00
abstract::We experimentally compare the performance and image contrast of spontaneous Raman and coherent Stokes Raman scattering microscopy. We demonstrate the differences between these techniques on a series of geometry-controlled samples that range in complexity from a point (array of tips) to one-dimensional (line grating) a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3533310
更新日期:2011-02-01 00:00:00
abstract::Discrimination of benign and malignant melanocytic lesions is a major issue in clinical dermatology. Assessment of the thickness of melanoma is critical for prognosis and treatment selection. We aimed to evaluate a novel optical computed tomography (optical-CT) system as a tool for three-dimensional (3-D) imaging of m...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.6.066004
更新日期:2012-06-01 00:00:00
abstract::We fabricate scaffolds with a fine linear grating (periodicity of 1 to 8 microm and height of 1 microm) on a glass substrate for controlling cardiomyocyte orientation. The fabrication is done by the solidification of a liquid photopolymerizable material using two laser beam interference. As the photopolymerizable mate...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2186042
更新日期:2006-03-01 00:00:00
abstract::Lumpectomy coupled with radiation therapy and/or chemotherapy is commonly used to treat breast cancer patients. We are developing an enhanced thermal IR imaging technique that has the potential to provide real-time imaging to guide tissue excision during a lumpectomy by delineating tumor margins. This enhanced thermal...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.11.116003
更新日期:2015-11-01 00:00:00
abstract::Noninvasive diagnostic tools, such as Trichoscan®, reflectance confocal microscopy (RCM), and optical coherence tomography (OCT), are efficient methods of hair shaft and growth evaluation. The aim of this study was to carry out a comparative assessment of these three medical procedures by measuring the hair shaft and ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,随机对照试验
doi:10.1117/1.JBO.17.10.101504
更新日期:2012-10-01 00:00:00
abstract::Smart toilets could be used to monitor different components of urine in daily life for early detection of lifestyle-related diseases and prompt provision of treatment. For analysis of biological samples such as urine by midinfrared spectroscopy, thin-film samples like liquid cells are needed because of the strong abso...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.JBO.23.5.050503
更新日期:2018-05-01 00:00:00