Abstract:
:We have applied wide-field digital interferometry (WFDI) to examine the morphology and dynamics of live red blood cells (RBCs) from individuals who suffer from sickle cell anemia (SCA), a genetic disorder that affects the structure and mechanical properties of RBCs. WFDI is a noncontact, label-free optical microscopy approach that can yield quantitative thickness profiles of RBCs and measurements of their membrane fluctuations at the nanometer scale reflecting their stiffness. We find that RBCs from individuals with SCA are significantly stiffer than those from a healthy control. Moreover, we show that the technique is sensitive enough to distinguish classes of RBCs in SCA, including sickle RBCs with apparently normal morphology, compared to the stiffer crescent-shaped sickle RBCs. We expect that this approach will be useful for diagnosis of SCA and for determining efficacy of therapeutic agents.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Shaked NT,Satterwhite LL,Telen MJ,Truskey GA,Wax Adoi
10.1117/1.3556717subject
Has Abstractpub_date
2011-03-01 00:00:00pages
030506issue
3eissn
1083-3668issn
1560-2281journal_volume
16pub_type
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