Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry.

Abstract:

:We have applied wide-field digital interferometry (WFDI) to examine the morphology and dynamics of live red blood cells (RBCs) from individuals who suffer from sickle cell anemia (SCA), a genetic disorder that affects the structure and mechanical properties of RBCs. WFDI is a noncontact, label-free optical microscopy approach that can yield quantitative thickness profiles of RBCs and measurements of their membrane fluctuations at the nanometer scale reflecting their stiffness. We find that RBCs from individuals with SCA are significantly stiffer than those from a healthy control. Moreover, we show that the technique is sensitive enough to distinguish classes of RBCs in SCA, including sickle RBCs with apparently normal morphology, compared to the stiffer crescent-shaped sickle RBCs. We expect that this approach will be useful for diagnosis of SCA and for determining efficacy of therapeutic agents.

journal_name

J Biomed Opt

authors

Shaked NT,Satterwhite LL,Telen MJ,Truskey GA,Wax A

doi

10.1117/1.3556717

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

030506

issue

3

eissn

1083-3668

issn

1560-2281

journal_volume

16

pub_type

信件
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