Abstract:
:Our long-term goal was to investigate the potential of incorporating redox imaging technique as a breast cancer (BC) diagnosis component to increase the positive predictive value of suspicious imaging finding and to reduce unnecessary biopsies and overdiagnosis. We previously found that precancer and cancer tissues in animal models displayed abnormal mitochondrial redox state. We also revealed abnormal mitochondrial redox state in cancerous specimens from three BC patients. Here, we extend our study to include biopsies of 16 patients. Tissue aliquots were collected from both apparently normal and cancerous tissues from the affected cancer-bearing breasts shortly after surgical resection. All specimens were snap-frozen and scanned with the Chance redox scanner, i.e., the three-dimensional cryogenic NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imager. We found both Fp and NADH in the cancerous tissues roughly tripled that in the normal tissues ( p < 0.05 ). The redox ratio Fp/(NADH + Fp) was ? 27 % higher in the cancerous tissues ( p < 0.05 ). Additionally, Fp, or NADH, or the redox ratio alone could predict cancer with reasonable sensitivity and specificity. Our findings suggest that the optical redox imaging technique can provide parameters independent of clinical factors for discriminating cancer from noncancer breast tissues in human patients.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Xu HN,Tchou J,Feng M,Zhao H,Li LZdoi
10.1117/1.JBO.21.11.114003subject
Has Abstractpub_date
2016-11-01 00:00:00pages
114003issue
11eissn
1083-3668issn
1560-2281pii
2589436journal_volume
21pub_type
杂志文章abstract::Fluorescence molecular tomography (FMT) is an important in vivo imaging modality to visualize physiological and pathological processes in small animals. However, FMT reconstruction is ill-posed and ill-conditioned due to strong optical scattering in deep tissues, which results in poor spatial resolution. It is well kn...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.5.055001
更新日期:2017-05-01 00:00:00
abstract::Lucigenin- and luminol-dependent chemiluminescence [(LC-CL) and (LM-CL)] in nondiluted human blood was studied. LM-CL was low in fresh blood and disappeared after its storage for 3 h, though the respiratory burst (RB) stimulation in blood was followed by high intensity and long-lasting LM-CL. LC-CL was high in fresh b...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429921
更新日期:1999-01-01 00:00:00
abstract::The infrared and Raman spectroscopy of bone and teeth tissues are reviewed. Characteristic spectra are obtained for both the mineral and protein components of these tissues. Vibrational spectroscopy is used to study the mineralization process, to define the chemical structure changes accompanying bone diseases, and to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.429994
更新日期:2000-07-01 00:00:00
abstract::In the present applications of optical coherence tomography (OCT), parameters besides pure morphology are evaluated in skin tissue under in vivo conditions. Spatially mapped refractive indices and scattering coefficients may support tissue characterization for research and diagnostic purposes in cosmetics/pharmacy and...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.429972
更新日期:2000-01-01 00:00:00
abstract::The results of an experimental study of the possibilities of monitoring erbium yttrium aluminum garnet laser-mediated ablation of human epidermis with the use of Fourier transform infrared (FTIR) spectroscopy and spectral polarization techniques are presented. The attenuated total reflection (ATR) method was used for ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1755719
更新日期:2004-07-01 00:00:00
abstract::The measurement of changes in blood volume in tissue is important for monitoring the effects of a wide range of therapeutic interventions, from radiation therapy to skin-flap transplants. Many systems available for purchase are either expensive or difficult to use, limiting their utility in the clinical setting. A low...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.10.105005
更新日期:2014-01-01 00:00:00
abstract::Optical technologies may be applied to multiple facets of spinal surgery from diagnostics to intraoperative image guidance to therapeutics. In diagnostics, the current standard remains cross-sectional static imaging. Optical surface scanning tools may have an important role; however, significant work is required to cl...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.JBO.23.6.060601
更新日期:2018-06-01 00:00:00
abstract::Imaging technologies working in the spatial frequency domain are becoming increasingly popular for generating wide-field maps of optical properties, enabling rapid analysis of tissue parameters. While acquisition methods have become faster and are now performing in real-time, processing methods remain slow, precluding...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.11.110501
更新日期:2016-11-01 00:00:00
abstract::Malaria parasites induce morphological, biochemical, and mechanical changes in red blood cells (RBCs). Mechanical variations are closely related to the deformability of individual RBCs. The deformation of various RBCs, including healthy and malaria-infected RBCs (iRBCs), can be directly observed through quantitative p...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.11.115003
更新日期:2015-11-01 00:00:00
abstract::Heart disease is the primary cause of death in the western world. Many of these deaths are caused by the rupture of vulnerable plaque. Vulnerable plaques are characterized by a large lipid core covered by a thin fibrous cap. One method for detecting these plaques is reflection spectroscopy. Several studies have invest...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2186332
更新日期:2006-03-01 00:00:00
abstract::Field carcinogenesis is the initial stage of cancer progression. Understanding field carcinogenesis is valuable for both cancer biology and clinical medicine. Here, we used inverse spectroscopic optical coherence tomography to study colorectal cancer (CRC) and pancreatic cancer (PC) field carcinogenesis. Depth-resolve...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.3.036013
更新日期:2014-03-01 00:00:00
abstract::A three-beam spectral domain optical coherence tomography system (OCT) whose center wavelength is 840 nm was developed. The three beams focus on fundus 3.1 mm apart from each other and are detected by a single line sensor. The distance between the beams is fixed and the beams scan a total area of 10×10 mm² while keep...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.10.106001
更新日期:2012-10-01 00:00:00
abstract::Time-resolved measurement of early arriving photons through diffusive media has been shown to effectively reduce the high degree of light scatter in biological tissue. However, the experimentally achievable reduction in photon scatter and the impact of time-gated detection on instrument noise performance is not well u...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3523371
更新日期:2010-11-01 00:00:00
abstract::We recently developed an algorithm for multiplexed fluorescence tomographic imaging of at least four fluorophores concurrently in the red and near-infrared wavelength region by jointly using spectral and temporal data. We report the design of a fluorescence tomography instrument that acquires spectral and temporal dat...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.10.105001
更新日期:2016-10-01 00:00:00
abstract::Toward developing a new method for restoring tissue viscoelasticity in scarred vocal folds, we previously proposed a method to localize biomaterials within subepithelial voids ablated using ultrafast laser pulses. The clinical implementation of this method necessitates the quantification of the laser parameters for ab...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.11.118001
更新日期:2013-11-01 00:00:00
abstract::We describe the use of a Teflon-AF liquid core optical fiber (LCOF) geometry to enhance the collection of Raman scattering from the biochemical creatinine, dissolved in water and in urine. At short integration times, where shot noise is most troublesome, the enhanced signal leads to greater accuracy in estimating the ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1917842
更新日期:2005-05-01 00:00:00
abstract::This paper presents an effective two-step scheme for automatic object detection in computed radiography (CR) images. First, various structure elements of the morphological filters, designed by incorporating available morphological features of the objects of interest including their sizes and rough shape descriptions, ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1289771
更新日期:2000-10-01 00:00:00
abstract::We present a study using plasmonic nanoparticles (NPs) to image epidermal growth factor receptor (EGFR) in live cells. Through detailed analysis of the NP scattering spectra, we determine the intracellular refractive index (RI) within attoliter volumes inside of the living cells. Molecular imaging is demonstrated usin...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2837450
更新日期:2008-01-01 00:00:00
abstract::According to the valence asymmetry hypothesis, the left/right asymmetry of prefrontal cortex (PFC) activity is correlated with specific emotional responses to mental stress and personality traits. Here, we evaluated the relation between emotional state and asymmetry in PFC activity at rest by using near-infrared spect...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.2.027005
更新日期:2014-02-01 00:00:00
abstract::We describe a computer eye model that allows for aspheric surfaces and a three-dimensional computer-based ray-tracing technique to simulate optical properties of the human eye and visual perception under various eye defects. Eye surfaces, such as the cornea, eye lens, and retina, are modeled or approximated by a set o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2357734
更新日期:2006-09-01 00:00:00
abstract::Fluorescence lifetime imaging (FLi) could potentially improve exogenous near-infrared (NIR) fluorescence imaging, because it offers the capability of discriminating a signal of interest from background, provides real-time monitoring of a chemical environment, and permits the use of several different fluorescent dyes h...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3368997
更新日期:2010-03-01 00:00:00
abstract::Treatment of deep venous thrombosis (DVT)--a primary cause of potentially fatal pulmonary embolism (PE)--depends on the age of the thrombus. The existing clinical imaging methods are capable of visualizing a thrombus but cannot determine the age of the blood clot. Therefore, there is a need for an imaging technique to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2992175
更新日期:2008-09-01 00:00:00
abstract::Bacterial contamination of blood products is one of the most frequent infectious complications of transfusion. Since glucose levels in blood supplies decrease as bacteria proliferate, it should be possible to detect the presence of bacterial contamination by measuring the glucose concentrations in the blood components...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.1.017004
更新日期:2012-01-01 00:00:00
abstract::Gap junction-mediated intercellular communication influences a variety of cellular activities. In tendons, gap junctions modulate collagen production, are involved in strain-induced cell death, and are involved in the response to mechanical stimulation. The aim of the present study was to investigate gap junction-medi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.1.015001
更新日期:2014-01-01 00:00:00
abstract::Cell morphology is often used as a valuable indicator of the physical condition and general status of living cells. We demonstrate a noninvasive method for morphological characterization of adherent cells. We measure infrared reflectivity spectrum at oblique angle from living cells cultured on thin Au film, and utiliz...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.8.081409
更新日期:2012-08-01 00:00:00
abstract::Surgical light is important for helping the surgeon easily identify specific tissues during an operation. We propose a spectral reflectance comparison model to optimize the light-emitting diode light spectrum in the operating room. An entropy evaluation method, meant specifically for surgical situations, was developed...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.10.105012
更新日期:2015-10-01 00:00:00
abstract::Discrimination of benign and malignant melanocytic lesions is a major issue in clinical dermatology. Assessment of the thickness of melanoma is critical for prognosis and treatment selection. We aimed to evaluate a novel optical computed tomography (optical-CT) system as a tool for three-dimensional (3-D) imaging of m...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.6.066004
更新日期:2012-06-01 00:00:00
abstract::We experimentally compare the performance and image contrast of spontaneous Raman and coherent Stokes Raman scattering microscopy. We demonstrate the differences between these techniques on a series of geometry-controlled samples that range in complexity from a point (array of tips) to one-dimensional (line grating) a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3533310
更新日期:2011-02-01 00:00:00
abstract::The lymphatic system is responsible for fluid homeostasis and immune cell trafficking and has been implicated in several diseases, including obesity, diabetes, and cancer metastasis. Despite its importance, the lack of suitable in vivo imaging techniques has hampered our understanding of the lymphatic system. This is,...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.10.106003
更新日期:2017-10-01 00:00:00
abstract::Near-infrared spectroscopy is a novel imaging technique potentially sensitive to both brain hemodynamics (slow signal) and neuronal activity (fast optical signal, FOS). The big challenge of measuring FOS noninvasively lies in the presumably low signal-to-noise ratio. Thus, detectability of the FOS has been controversi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3505007
更新日期:2010-11-01 00:00:00