Development of single-channel optical video stereomicroscopy.

abstract：:We investigate the relationship between the laser beam at the retina (spot size) and the extent of retinal injury from single ultrashort laser pulses. From previous studies it is believed that the retinal effect of single 3-ps laser pulses should vary in extent and location, depending on the occurrence of laser-induce...

journal_title：Journal of biomedical optics

authors： Roach WP,Cain CP,Narayan DG,Noojin GD,Boppart SA,Birngruber R,Fujimoto JG,Toth CA

更新日期：2004-11-01 00:00:00

abstract：:We demonstrate the first application of Raman spectroscopy in diagnosing nonmalignant, premalignant, malignant, and metastatic stages of breast cancer in a three-dimensional (3-D) cell culture model that closely mimics an in vivo environment. Comprehensive study comparing classification in two-dimensional (2-D) and 3-...

journal_title：Journal of biomedical optics

authors： Damayanti NP,Fang Y,Parikh MR,Craig AP,Kirshner J,Irudayaraj J

更新日期：2013-11-01 00:00:00

abstract：:A stable optical system is required to acquire a high-quality image. A motionless lensless setup is designated to obtain high-resolution and large field of view images. The sample is sequentially illuminated with multiple random phase patterns, and the recorded images are subtracted from the system calibration images ...

journal_title：Journal of biomedical optics

authors： Hussain A,Li Y,Liu D,Kuang C,Liu X

更新日期：2017-11-01 00:00:00

abstract：:Voltage-sensitive dyes (VSDs) are designed to monitor membrane potential by detecting fluorescence changes in response to neuronal or muscle electrical activity. However, fluorescence imaging is limited by depth of penetration and high scattering losses, which leads to low sensitivity in vivo systems for external dete...

journal_title：Journal of biomedical optics

authors： Zhang HK,Yan P,Kang J,Abou DS,Le HN,Jha AK,Thorek DL,Kang JU,Rahmim A,Wong DF,Boctor EM,Loew LM

更新日期：2017-04-01 00:00:00

abstract：:We describe a systematic approach to image, track, and quantify the movements of HIV viruses embedded in human cervical mucus. The underlying motivation for this study is that, in HIV-infected adults, women account for more than half of all new cases and most of these women acquire the infection through heterosexual c...

journal_title：Journal of biomedical optics

authors： Boukari F,Makrogiannis S,Nossal R,Boukari H

更新日期：2016-09-01 00:00:00

abstract：:Computer simulation is used to facilitate the design of fiber-probe geometries that enable enhanced detection of optical signals arising from specific tissue depths. Obtaining understanding of the relationship between fiber-probe design and tissue interrogation is critical when developing strategies for optical detect...

journal_title：Journal of biomedical optics

authors： Wang AM,Bender JE,Pfefer J,Utzinger U,Drezek RA

更新日期：2005-07-01 00:00:00

abstract：:Traditional white-light and fluorescent imaging techniques provide powerful methods to extract high-resolution information from two-dimensional (2-D) sections, but to retrieve information from a three-dimensional (3-D) volume they require relatively slow scanning methods that result in increased acquisition time. Usin...

journal_title：Journal of biomedical optics

authors： Duocastella M,Sun B,Arnold CB

更新日期：2012-05-01 00:00:00

abstract：:The spectroscopy of analyte-specific molecular vibrations in tissue thin sections has opened up a path toward histopathology without the need for tissue staining. However, biomedical vibrational imaging has not yet advanced from academic research to routine histopathology due to long acquisition times for the microsco...

journal_title：Journal of biomedical optics

authors： Kröger N,Egl A,Engel M,Gretz N,Haase K,Herpich I,Kränzlin B,Neudecker S,Pucci A,Schönhals A,Vogt J,Petrich W

更新日期：2014-01-01 00:00:00

abstract：:Intracellular oxygen concentration is of primary importance in determining numerous physiological and pathological processes in biological systems. In this paper, we describe the application of the oxygen sensing indicator, ruthenium dibipyridine 4-(1"-pyrenyl)-2,2'-bipyridine chloride [Ru(bpy-pyr)(bpy)(2)], for molec...

journal_title：Journal of biomedical optics

authors： Ji J,Rosenzweig N,Jones I,Rosenzweig Z

更新日期：2002-07-01 00:00:00

abstract：:Functional near-infrared spectroscopy (fNIRS) is being increasingly applied to affective and social neuroscience research; however, the reliability of this method is still unclear. This study aimed to evaluate the test–retest reliability of the fNIRS-based prefrontal response to emotional stimuli. Twenty-six participa...

journal_title：Journal of biomedical optics

authors： Huang Y,Mao M,Zhang Z,Zhou H,Zhao Y,Duan L,Kreplin U,Xiao X,Zhu C

更新日期：2017-01-01 00:00:00

abstract：:The analysis of biological systems by spectroscopic techniques involves the evaluation of hundreds to thousands of variables. Hence, different statistical approaches are used to elucidate regions that discriminate classes of samples and to propose new vibrational markers for explaining various phenomena like disease m...

journal_title：Journal of biomedical optics

authors： Oliveira Mendes Td,Pinto LP,Santos LD,Tippavajhala VK,Téllez Soto CA,Martin AA

更新日期：2016-07-01 00:00:00

abstract：:We present for the first time in vivo full three-dimensional (3-D) photoacoustic tomography (PAT) of the distal interphalangeal joint in a human subject. Both absorbed energy density and absorption coefficient images of the joint are quantitatively obtained using our finite-element-based photoacoustic image reconstruc...

journal_title：Journal of biomedical optics

authors： Sun Y,Sobel E,Jiang H

更新日期：2009-11-01 00:00:00

abstract：:A Monte-Carlo-based model of fluorescence is developed that is capable of extracting the intrinsic fluorescence properties of tissue, which are independent of the absorption and scattering properties of tissue. This model is flexible in its applicability to different illumination-collection geometries and is also vali...

journal_title：Journal of biomedical optics

authors： Palmer GM,Ramanujam N

更新日期：2008-03-01 00:00:00

abstract：:A fully integrated trimodality fluorescence, diffuse optical, and x-ray computed tomography (FT/DOT/XCT) system for small animal imaging is reported in this work. The main purpose of this system is to obtain quantitatively accurate fluorescence concentration images using a multimodality approach. XCT offers anatomical...

journal_title：Journal of biomedical optics

authors： Lin Y,Barber WC,Iwanczyk JS,Roeck WW,Nalcioglu O,Gulsen G

更新日期：2010-07-01 00:00:00

abstract：:As small animal optical imaging and tomography are gaining popularity for interrogating functional and molecular events in vivo, it becomes increasingly necessary to gain knowledge of the optical properties of the species investigated to better understand and describe photon propagation through their tissues. To achie...

journal_title：Journal of biomedical optics

authors： Niedre MJ,Turner GM,Ntziachristos V

更新日期：2006-11-01 00:00:00

abstract：:To evaluate retinal perfusion in the human eye, a dual-wavelength confocal scanning laser ophthalmoscope (cSLO) was developed that provides spectral imaging of the fundus using a combination of red (670 nm) and near-infrared (810 nm) wavelengths. The image of the ocular fundus was analyzed to find out if quantitative ...

journal_title：Journal of biomedical optics

authors： Rasta SH,Manivannan A,Sharp PF

更新日期：2012-11-01 00:00:00

abstract：:Previous studies have shown the ability of many lymphatic vessels to contract phasically to pump lymph. Every lymphangion can act like a heart with pacemaker sites that initiate the phasic contractions. The contractile wave propagates along the vessel to synchronize the contraction. However, determining the location o...

journal_title：Journal of biomedical optics

authors： Akl TJ,Nepiyushchikh ZV,Gashev AA,Zawieja DC,Cot GL

更新日期：2011-02-01 00:00:00

abstract：:The potential of intrinsic optical imaging and resting-state analysis under anesthetized conditions as a tool to study brain networks associated with epileptic seizures is investigated. Using an acute model of epileptiform activity, the 4-aminopyridine model in live mice, we observe the changes in resting-state networ...

journal_title：Journal of biomedical optics

authors： Guevara E,Pouliot P,Nguyen DK,Lesage F

更新日期：2013-07-01 00:00:00

abstract：:To improve surgical guidance toward prostate draining lymph nodes, we investigate the potential of intraoperative fluorescence imaging and combined pre- and intraoperative multimodality imaging approaches. Transgenic adenocarcinoma mouse prostate mice with spontaneous prostate tumors are injected intratumorally with: ...

journal_title：Journal of biomedical optics

authors： van Leeuwen AC,Buckle T,Bendle G,Vermeeren L,Valdés Olmos R,van de Poel HG,van Leeuwen FW

更新日期：2011-01-01 00:00:00

abstract：:We describe the implementation and use of an adaptive optics loop in the imaging path of a commercial wide field microscope. We show that it is possible to maintain the optical performances of the original microscope when imaging through aberrant biological samples. The sources used for illuminating the adaptive optic...

journal_title：Journal of biomedical optics

authors： Vermeulen P,Muro E,Pons T,Loriette V,Fragola A

更新日期：2011-07-01 00:00:00

abstract：:A wide variety of ocular diseases are associated with abnormalities in ocular circulation. As such, there is considerable interest in techniques for quantifying retinal blood flow, among which Doppler optical coherence tomography (OCT) may be the most promising. We present an approach to measure retinal blood flow in ...

journal_title：Journal of biomedical optics

authors： Werkmeister RM,Vietauer M,Knopf C,Fürnsinn C,Leitgeb RA,Reitsamer H,Gröschl M,Garhöfer G,Vilser W,Schmetterer L

更新日期：2014-01-01 00:00:00

abstract：:Fast estimation of optical properties from reflectance measurements at two spatial frequencies could pave way for real-time, wide-field and quantitative mapping of vital signs of tissues. We present a machine learning-based approach for estimating optical properties in the spatial frequency domain, where a random fore...

journal_title：Journal of biomedical optics

authors： Panigrahi S,Gioux S

更新日期：2018-12-01 00:00:00

abstract：:The objective of this work was to compare the accuracy of analyte concentration estimation when using transmission versus diffuse reflectance spectroscopy of a scattering medium. Monte Carlo ray tracing of light through the medium was used in conjunction with pure component absorption spectra and Beer-Lambert absorpti...

journal_title：Journal of biomedical optics

authors： Gardner CM

更新日期：2018-01-01 00:00:00

abstract：:Image correlation spectroscopy (ICS) is a powerful technique for detecting arrangement of fluorophores in images. This tutorial gives background into the mathematical underpinnings of ICS, specifically image autocorrelation. The effects of various artifacts and image processing steps, including background subtraction,...

journal_title：Journal of biomedical optics

authors： Robertson C,George SC

更新日期：2012-08-01 00:00:00

abstract：:Cell morphology is often used as a valuable indicator of the physical condition and general status of living cells. We demonstrate a noninvasive method for morphological characterization of adherent cells. We measure infrared reflectivity spectrum at oblique angle from living cells cultured on thin Au film, and utiliz...

journal_title：Journal of biomedical optics

authors： Yashunsky V,Lirtsman V,Zilbershtein A,Bein A,Schwartz B,Aroeti B,Golosovsky M,Davidov D

更新日期：2012-08-01 00:00:00

abstract：:A side-viewing, 2.3-mm diameter oblique incidence reflectometry endoscope has been designed to obtain optical property measurements of turbid samples. Light from a single-mode fiber is relayed obliquely onto the tissue with a gradient index lens-based distal optics assembly and the resulting diffuse reflectance profil...

journal_title：Journal of biomedical optics

authors： Wall RA,Barton JK

更新日期：2014-06-01 00:00:00

abstract：:We propose a handheld projective imaging device for orthotopic projection of near-infrared fluorescence images onto target biological tissue at visible wavelengths without any additional visual aid. The device integrates a laser diode light source module, a camera module, a projector, an ultrasonic distance sensor, a ...

journal_title：Journal of biomedical optics

authors： Li C,Liu P,Shao P,Pei J,Li Y,Pawlik TM,Martin EW,Xu RX

更新日期：2019-08-01 00:00:00

abstract：:Three major analytical tools in imaging science are summarized and demonstrated relative to optical imaging in vivo. Standard resolution testing is optimal when infinite contrast is used and hardware evaluation is the goal. However, deep tissue imaging of absorption or fluorescent contrast agents in vivo often present...

journal_title：Journal of biomedical optics

authors： Pogue BW,Davis SC,Song X,Brooksby BA,Dehghani H,Paulsen KD

更新日期：2006-05-01 00:00:00

abstract：:In-vivo imaging of the vascular system can provide novel insight into the dynamics of vasoconstriction and vasodilation. Fourier domain optical coherence tomography (FD-OCT) is an optical, noncontact imaging technique based on interferometry of short-coherent near-infrared light with axial resolution of less than 10 m...

journal_title：Journal of biomedical optics

authors： Meissner S,Müller G,Walther J,Morawietz H,Koch E

更新日期：2009-05-01 00:00:00

abstract：:Spatially resolved diffuse reflectance spectroscopy (srDRS) is a well-established technique for noninvasive, in vivo characterization of tissue optical properties toward diagnostic applications. srDRS has a potential for depth-resolved analysis of tissue, which is desired in various clinical situations. However, curre...

journal_title：Journal of biomedical optics

authors： Petitdidier N,Koenig A,Gerbelot R,Grateau H,Gioux S,Jallon P

更新日期：2018-11-01 00:00:00