Abstract:
:Voltage-sensitive dyes (VSDs) are designed to monitor membrane potential by detecting fluorescence changes in response to neuronal or muscle electrical activity. However, fluorescence imaging is limited by depth of penetration and high scattering losses, which leads to low sensitivity in vivo systems for external detection. By contrast, photoacoustic (PA) imaging, an emerging modality, is capable of deep tissue, noninvasive imaging by combining near-infrared light excitation and ultrasound detection. Here, we show that voltage-dependent quenching of dye fluorescence leads to a reciprocal enhancement of PA intensity. We synthesized a near-infrared photoacoustic VSD (PA-VSD), whose PA intensity change is sensitive to membrane potential. In the polarized state, this cyanine-based probe enhances PA intensity while decreasing fluorescence output in a lipid vesicle membrane model. A theoretical model accounts for how the experimental PA intensity change depends on fluorescence and absorbance properties of the dye. These results not only demonstrate PA voltage sensing but also emphasize the interplay of both fluorescence and absorbance properties in the design of optimized PA probes. Together, our results demonstrate PA sensing as a potential new modality for recording and external imaging of electrophysiological and neurochemical events in the brain.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Zhang HK,Yan P,Kang J,Abou DS,Le HN,Jha AK,Thorek DL,Kang JU,Rahmim A,Wong DF,Boctor EM,Loew LMdoi
10.1117/1.JBO.22.4.045006subject
Has Abstractpub_date
2017-04-01 00:00:00pages
45006issue
4eissn
1083-3668issn
1560-2281pii
2618459journal_volume
22pub_type
杂志文章abstract::The objective of this work was to compare the accuracy of analyte concentration estimation when using transmission versus diffuse reflectance spectroscopy of a scattering medium. Monte Carlo ray tracing of light through the medium was used in conjunction with pure component absorption spectra and Beer-Lambert absorpti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.1.018001
更新日期:2018-01-01 00:00:00
abstract::We investigate the relationship between the laser beam at the retina (spot size) and the extent of retinal injury from single ultrashort laser pulses. From previous studies it is believed that the retinal effect of single 3-ps laser pulses should vary in extent and location, depending on the occurrence of laser-induce...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1805554
更新日期:2004-11-01 00:00:00
abstract::In all fundus laser treatments of the eye, the temperature increase is not exactly known. In order to optimize treatments, an online temperature determination is preferable. We investigated a noninvasive optoacoustic method to monitor the fundus temperature during pulsed laser irradiation. When laser pulses are applie...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1627338
更新日期:2004-01-01 00:00:00
abstract::We present a method for lung cancer detection exploiting reflectance spectra measured in vivo during endoscopic imaging of the lung. The measured reflectance spectra were analyzed using a specially developed light-transport model to obtain quantitative information about cancer-related, physiological, and morphologic c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2337529
更新日期:2006-07-01 00:00:00
abstract::Diffusion of two model drugs-benzyl nicotinate and ibuprofen-and the plasma macromolecule albumin across atherosclerotic rabbit aorta was studied ex vivo by attenuated total reflection-Fourier transform infrared (ATR-FTIR) imaging. Solutions of these molecules were applied to the endothelial surface of histological se...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3174395
更新日期:2009-07-01 00:00:00
abstract::An integrated miniature multi-modal microscope (4M device) for microendoscopy was built and tested. Imaging performance is evaluated and imaging results are presented for both fluorescence and reflectance samples. Images of biological samples show successful imaging of both thin layers of fixed cells prepared on a sli...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2978060
更新日期:2008-09-01 00:00:00
abstract::We have developed a near-infrared (NIR) fluorescence goggle system based on the complementary metal-oxide-semiconductor active pixel sensor imaging and see-through display technologies. The fluorescence goggle system is a compact wearable intraoperative fluorescence imaging and display system that can guide surgery in...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.10.101303
更新日期:2013-10-01 00:00:00
abstract::Blood smear analysis has remained a crucial diagnostic tool for pathologists despite the advent of automatic analyzers such as flow cytometers and impedance counters. Though these current methods have proven to be indispensible tools for physicians and researchers alike, they provide limited information on the detaile...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3369965
更新日期:2010-03-01 00:00:00
abstract::The specificity of the hemodynamic response function (HRF) is determined spatially by the vascular architecture and temporally by the evolution of hemodynamic changes. Here, we used functional photoacoustic microscopy (fPAM) to investigate single cerebral blood vessels of rats after left forepaw stimulation. In this s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.6.061210
更新日期:2012-06-01 00:00:00
abstract::Full-field optical coherence tomography (FF-OCT) capable of in vivo cellular-level imaging is demonstrated for nonscanning horizontal cross-sectional imaging. The system is based on a white light interference microscope illuminated by a thermal light source. A dual-channel two-dimensional (2-D) detection technique inc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2822159
更新日期:2007-11-01 00:00:00
abstract::Limited cutaneous systemic sclerosis (lcSSc) was formerly known as CREST syndrome in reference to the associated clinical features: calcinosis, Raynaud's phenomenon, esophageal dysfunction, sclerodactyly, and telangiectasias. The transforming growth factor beta has been identified as a major player in the pathogenic p...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.11.118001
更新日期:2014-01-01 00:00:00
abstract::Mueller polarimetry is applied to study the samples of nails: natural (or reference) and irradiated to 2 Gy ionizing radiation dose. We measure the whole Mueller matrices of the samples as a function of the scattering angle at a wavelength of 632.8 nm. We apply depolarization analysis to measured Mueller matrices by c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.7.071108
更新日期:2016-07-01 00:00:00
abstract::Ex vivo human skin has been used extensively for cosmeceutical and drug delivery studies, transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to ischemic necrosis. Traditional methods of assessing viability can be time-consuming and provide limited metabolic information. Using ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3466580
更新日期:2010-07-01 00:00:00
abstract::We describe a computer eye model that allows for aspheric surfaces and a three-dimensional computer-based ray-tracing technique to simulate optical properties of the human eye and visual perception under various eye defects. Eye surfaces, such as the cornea, eye lens, and retina, are modeled or approximated by a set o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2357734
更新日期:2006-09-01 00:00:00
abstract:SIGNIFICANCE:Detailed biochemical and morphological imaging of the plaque burdened coronary arteries holds the promise of improved understanding of atherosclerosis plaque development, ultimately leading to better diagnostics and therapies. AIM:Development of a dual-modality intravascular catheter supporting swept-sour...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.9.096010
更新日期:2020-09-01 00:00:00
abstract::Fast estimation of optical properties from reflectance measurements at two spatial frequencies could pave way for real-time, wide-field and quantitative mapping of vital signs of tissues. We present a machine learning-based approach for estimating optical properties in the spatial frequency domain, where a random fore...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.7.071606
更新日期:2018-12-01 00:00:00
abstract::Angle-resolved signals of polarized light scattered by biological cells provide rich information on cell morphology. Quantitative study of these signals can lead to new methods to develop and improve high-throughput instrumentation for cell probing such as scattering-based flow cytometry. We employ a goniometer system...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2749730
更新日期:2007-05-01 00:00:00
abstract::We have been developing a Mach-Zehnder type of optical frequency domain reflectometry optical coherence tomography (OFDR-OCT) that uses discretely swept superstructure-grating distributed Bragg- reflector (SSG-DBR) lasers developed for telecommunication fields and has a 12-mm-depth range. We report images obtained wit...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2839042
更新日期:2008-01-01 00:00:00
abstract::We present a quantitative analysis of dynamic diffuse optical measurements to obtain oxygen saturation of hemoglobin in volume oscillating compartments. We used a phasor representation of oscillatory hemodynamics at the heart rate and respiration frequency to separate the oscillations of tissue concentrations of oxyhe...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.10.101408
更新日期:2016-10-01 00:00:00
abstract::Common methods to characterize treatment efficacy based on morphological imaging may misrepresent outcomes and exclude effective therapies. Using a three-dimensional model of ovarian cancer, two functional treatment response metrics are used to evaluate photodynamic therapy (PDT) efficacy: total volume, calculated fro...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.9.098004
更新日期:2013-09-01 00:00:00
abstract::This paper presents a fiber Bragg grating (FBG)-instrumented prosthetic silicone liner that provides cushioning for the residual limb and can successfully measure interface pressures inside prosthetic sockets of lower-limb amputees in a simple and practical means of sensing. The liner is made of two silicone layers be...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.8.087001
更新日期:2017-08-01 00:00:00
abstract::Motion artifacts are the most significant sources of noise in the context of pediatric brain imaging designs and data analyses, especially in applications of functional near-infrared spectroscopy (fNIRS), in which it can completely affect the quality of the data acquired. Different methods have been developed to corre...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.12.126003
更新日期:2015-01-01 00:00:00
abstract::Fluorescence image-guided surgery (FIGS) is an emerging technique in oncology, neurology, and cardiology. To adapt intraoperative imaging for various surgical applications, increasingly flexible and compact FIGS instruments are necessary. We present a compact, portable FIGS system and demonstrate its use in cardiovasc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3378128
更新日期:2010-03-01 00:00:00
abstract::We have developed a novel nitroimidazole indocyanine dye conjugate for tumor-targeted hypoxia fluorescence tomography. The hypoxia probe has been evaluated in vitro using tumor cell lines and in vivo with tumor targeting in mice. The in vitro cell studies were performed to assess fluorescence labeling differences betw...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3589348
更新日期:2011-06-01 00:00:00
abstract::Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spont...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.3.035001
更新日期:2016-03-01 00:00:00
abstract::A fully integrated trimodality fluorescence, diffuse optical, and x-ray computed tomography (FT/DOT/XCT) system for small animal imaging is reported in this work. The main purpose of this system is to obtain quantitatively accurate fluorescence concentration images using a multimodality approach. XCT offers anatomical...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3467495
更新日期:2010-07-01 00:00:00
abstract::A transgenic mouse model with a Clim [co-factor of LIM (a combination of first letters of Lin-11 (C. elegans), ISL1 (rat), and Mec-3 (C. elegans) gene names) domain proteins] gene partially blocked in the epithelial compartment of its tissues is used to establish the sensitivity of intrinsic reflectance nonlinear opti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2163254
更新日期:2006-01-01 00:00:00
abstract::Optical coherence tomography (OCT) is an emergent imaging tool used for noninvasive diagnosis of skin diseases. The present meta-analysis was carried out to assess the accuracy of OCT for the diagnosis of skin cancer. We conducted a systematic literature search though EMBASE, Medline, PubMed, the Cochrane Library, and...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,meta分析
doi:10.1117/1.JBO.23.2.020902
更新日期:2018-02-01 00:00:00
abstract::To evaluate retinal perfusion in the human eye, a dual-wavelength confocal scanning laser ophthalmoscope (cSLO) was developed that provides spectral imaging of the fundus using a combination of red (670 nm) and near-infrared (810 nm) wavelengths. The image of the ocular fundus was analyzed to find out if quantitative ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.11.116005
更新日期:2012-11-01 00:00:00
abstract:SIGNIFICANCE:The blood-brain barrier (BBB) is a major obstacle to detecting and treating brain tumors. Overcoming this challenge will facilitate the early and accurate detection of brain lesions and guide surgical resection of tumors. AIM:We generated an orthotopic brain tumor model that simulates the pathophysiology ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.2.026004
更新日期:2020-02-01 00:00:00