Quantum cascade laser-based hyperspectral imaging of biological tissue.

abstract：:The development of accurate and clinically applicable tools to assess cancer risk is essential to define candidates to undergo screening for early-stage cancers at a curable stage or provide a novel method to monitor chemoprevention treatments. With the use of our recently developed optical technology--spatial-domain ...

journal_title：Journal of biomedical optics

authors： Bista RK,Uttam S,Hartman DJ,Qiu W,Yu J,Zhang L,Brand RE,Liu Y

更新日期：2012-06-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) in tumor cells was found to play an important role in maintaining the malignant phenotype. Using laser scanning confocal fluorescence microscopy (LSCFM) in a recent work, we reported a variable fluorescence intensity of ethidium bromide (EB) in mitochondria nucleoids of living carcinoma cells...

journal_title：Journal of biomedical optics

authors： Villa AM,Fusi P,Pastori V,Amicarelli G,Pozzi C,Adlerstein D,Doglia SM

更新日期：2012-04-01 00:00:00

abstract：:We present a simple wide-field imaging technique, called HiLo microscopy, that is capable of producing optically sectioned images in real time, comparable in quality to confocal laser scanning microscopy. The technique is based on the fusion of two raw images, one acquired with speckle illumination and another with st...

journal_title：Journal of biomedical optics

authors： Lim D,Ford TN,Chu KK,Mertz J

更新日期：2011-01-01 00:00:00

abstract：:Optically derived tissue properties across a range of breast densities and the effects of breast compression on estimates of hemoglobin, oxygen metabolism, and water and lipid concentrations were obtained from a coregistered imaging system that integrates near-infrared spectral tomography (NIRST) with digital breast t...

journal_title：Journal of biomedical optics

authors： Michaelsen KE,Krishnaswamy V,Shi L,Vedantham S,Karellas A,Pogue BW,Paulsen KD,Poplack SP

更新日期：2016-09-01 00:00:00

abstract：:Surgical light is important for helping the surgeon easily identify specific tissues during an operation. We propose a spectral reflectance comparison model to optimize the light-emitting diode light spectrum in the operating room. An entropy evaluation method, meant specifically for surgical situations, was developed...

journal_title：Journal of biomedical optics

authors： Shen J,Wang H,Wu Y,Li A,Chen C,Zheng Z

更新日期：2015-10-01 00:00:00

abstract：:Type I collagen is a major component of the extracellular matrix as well as many tissue engineered models. To understand changes in collagen related models over time, it is important to evaluate collagen dynamics with noninvasive techniques. Fluorescence spectroscopy provides a method to noninvasively measure endogeno...

journal_title：Journal of biomedical optics

authors： Kirkpatrick ND,Hoying JB,Botting SK,Weiss JA,Utzinger U

更新日期：2006-09-01 00:00:00

abstract：:We fabricate scaffolds with a fine linear grating (periodicity of 1 to 8 microm and height of 1 microm) on a glass substrate for controlling cardiomyocyte orientation. The fabrication is done by the solidification of a liquid photopolymerizable material using two laser beam interference. As the photopolymerizable mate...

journal_title：Journal of biomedical optics

authors： Fujita A,Fujita K,Nakamura O,Matsuda T,Kawata S

更新日期：2006-03-01 00:00:00

abstract：:We report the design of a novel laser line-triangulation laryngoscope for the quantitative visualization of the three-dimensional movements of human vocal folds during phonation. This is the first successful in vivo recording of the three-dimensional movements of human vocal folds in absolute values. Triangulation ima...

journal_title：Journal of biomedical optics

authors： George NA,de Mul FF,Qiu Q,Rakhorst G,Schutte HK

更新日期：2008-11-01 00:00:00

abstract：:The measurement of changes in blood volume in tissue is important for monitoring the effects of a wide range of therapeutic interventions, from radiation therapy to skin-flap transplants. Many systems available for purchase are either expensive or difficult to use, limiting their utility in the clinical setting. A low...

journal_title：Journal of biomedical optics

authors： Glennie DL,Hayward JE,McKee DE,Farrell TJ

更新日期：2014-01-01 00:00:00

abstract：:A synchrotron light source dedicated to medical applications has been designed at National Institute of Radiological Sciences. The storage ring, with circumference of 80 m, is designed for acceleration of 2.3 GeV and a stored current of 420 mA. It is equipped with two multipole wigglers to produce sufficient photon fl...

journal_title：Journal of biomedical optics

authors： Torikoshi M,Tsunoo T,Endo M,Noda K,Kumada M,Yamada S,Soga F,Hyodo K

更新日期：2001-07-01 00:00:00

abstract：:Penetration profiles of topically applied drugs and cosmetic products provide important information on their efficacy. The application of tape stripping in combination with UV/VIS spectroscopy is checked to determine the local position of topically applied substances inside the stratum corneum, the penetration profile...

journal_title：Journal of biomedical optics

authors： Weigmann HJ,Jacobi U,Antoniou C,Tsikrikas GN,Wendel V,Rapp C,Gers-Barlag H,Sterry W,Lademann J

更新日期：2005-01-01 00:00:00

abstract：:We propose a rate process model for describing photochemical damage to retinal cells by short wavelength laser exposures. The rate equation for photochemical damage contains a positive rate that is temperature independent, and a negative (quenching) rate that is temperature dependent. Using the traditional Arrhenius i...

journal_title：Journal of biomedical optics

authors： Clark CD 3rd,Denton ML,Thomas RJ

更新日期：2011-02-01 00:00:00

abstract：:The nEUROPt protocol is one of two new protocols developed within the European project nEUROPt to characterize the performances of time-domain systems for optical imaging of the brain. It was applied in joint measurement campaigns to compare the various instruments and to assess the impact of technical improvements. T...

journal_title：Journal of biomedical optics

authors： Wabnitz H,Jelzow A,Mazurenka M,Steinkellner O,Macdonald R,Milej D,Żołek N,Kacprzak M,Sawosz P,Maniewski R,Liebert A,Magazov S,Hebden J,Martelli F,Di Ninni P,Zaccanti G,Torricelli A,Contini D,Re R,Zucchelli L,Spine

更新日期：2014-08-01 00:00:00

abstract：:Biomedical applications of terahertz (THz) radiation are appealing because THz radiation is nonionizing and has the demonstrated ability to detect intrinsic contrasts between cancerous and normal tissue. A linear polarization-sensitive detection technique for tumor margin delineation has already been demonstrated; how...

journal_title：Journal of biomedical optics

authors： Martin JP,Joseph CS,Giles RH

更新日期：2016-07-01 00:00:00

abstract：:Knowledge of neuronal wiring and morphogenesis in Drosophila is essential to understand brain function and dysfunction. The immunoenzyme method based on horseradish peroxidase/diaminobenzidine (HRP/DAB) provides high-contrast images to resolve details underlying neuronal architecture. However, the poor staining penetr...

journal_title：Journal of biomedical optics

authors： Tian Q,Yuan J,Li Y,Jiang T,Gong H,Zhou W

更新日期：2014-09-01 00:00:00

abstract：:Dynamic analyses of vibrational motion in cell membranes provide a lot of information on the complex dynamic motilities of a red blood cell (RBC). Here, we present the correlation properties of membrane fluctuation in discocyte and spherocyte RBCs by using quantitative phase microscopy (QPM). Since QPM can provide nan...

journal_title：Journal of biomedical optics

authors： Lee S,Lee JY,Park CS,Kim DY

更新日期：2011-07-01 00:00:00

abstract：:In laser retinal photocoagulation, short (<20 ms) pulses have been found to reduce thermal damage to the inner retina, decrease treatment time, and minimize pain. However, the safe therapeutic window (defined as the ratio of power for producing a rupture to that of mild coagulation) decreases with shorter exposures. T...

journal_title：Journal of biomedical optics

authors： Sramek C,Paulus Y,Nomoto H,Huie P,Brown J,Palanker D

更新日期：2009-05-01 00:00:00

abstract：:Spasticity is a motor disorder frequently present in individuals with cerebral palsy (CP). This study aimed to evaluate the effect of low-level laser therapy (LLLT) on the spasticity of the masseter and anterior temporal muscle fibers in children with CP over three weeks of intermittent laser exposures. The bite force...

journal_title：Journal of biomedical optics

authors： Santos MT,Diniz MB,Gouw-Soares SC,Lopes-Martins RA,Frigo L,Baeder FM

更新日期：2016-02-01 00:00:00

abstract：:In near-infrared spectroscopy, concentration changes in oxygenated and deoxygenated hemoglobin are calculated from the changes in the attenuation of the measurement light. This is done by solving a linear equation based on the modified Lambert-Beer law. To solve this equation, we need to know the partial optical pathl...

journal_title：Journal of biomedical optics

authors： Umeyama S,Yamada T

更新日期：2009-05-01 00:00:00

abstract：:We present a hybrid method that combines a multilayered scaling method and a perturbation method to speed up the Monte Carlo simulation of diffuse reflectance from a multilayered tissue model with finite-size tumor-like heterogeneities. The proposed method consists of two steps. In the first step, a set of photon traj...

journal_title：Journal of biomedical optics

authors： Zhu C,Liu Q

更新日期：2012-01-01 00:00:00

abstract：:The capability of optical coherence tomography (OCT) to perform "optical biopsy" of tissues within a depth range of 1 to 2 mm with micron-scale resolution in real time makes it a promising biomedical imaging modality for dermatologic applications. Three high-speed, spectrometer-based frequency-domain OCT systems opera...

journal_title：Journal of biomedical optics

authors： Alex A,Povazay B,Hofer B,Popov S,Glittenberg C,Binder S,Drexler W

更新日期：2010-03-01 00:00:00

abstract：:Time-deconvolution of the instrument response from fluorescence lifetime imaging microscopy (FLIM) data is usually necessary for accurate fluorescence lifetime estimation. In many applications, however, the instrument response is not available. In such cases, a blind deconvolution approach is required. An iterative me...

journal_title：Journal of biomedical optics

authors： Campos-Delgado DU,Gutierrez-Navarro O,Arce-Santana ER,Skala MC,Walsh AJ,Jo JA

更新日期：2015-07-01 00:00:00

abstract：:A primary focus of neurointensive care is monitoring the injured brain to detect harmful events that can impair cerebral blood flow (CBF), resulting in further injury. Since current noninvasive methods used in the clinic can only assess blood flow indirectly, the goal of this research is to develop an optical techniqu...

journal_title：Journal of biomedical optics

authors： Diop M,Tichauer KM,Elliott JT,Migueis M,Lee TY,St Lawrence K

更新日期：2010-09-01 00:00:00

abstract：:Breast cancer is one of the most common cancers in females. To monitor chemotherapeutic efficacy for breast cancer, medical imaging systems such as x-ray mammography, computed tomography, magnetic resonance imaging, and ultrasound imaging have been used. Currently, it can take up to 3 to 6 weeks to see the tumor respo...

journal_title：Journal of biomedical optics

authors： Lee S,Jeong H,Seong M,Kim JG

更新日期：2017-12-01 00:00:00

abstract：:Laser speckle contrast imaging is a technique used for imaging blood flow without scanning. Though several studies have attempted to combine spatial and temporal statistics of laser speckle images for reducing image noise as well as preserving acceptable spatiotemporal resolution, the statistical accuracy of these spa...

journal_title：Journal of biomedical optics

authors： Qiu J,Li P,Luo W,Wang J,Zhang H,Luo Q

更新日期：2010-01-01 00:00:00

abstract：:Peripheral nerve injury in vivo promotes a regenerative growth in vitro characterized by an improved neurite regrowth. Knowledge of the conditioning injury effects on both morphology and mechanical properties of live sensory neurons could be instrumental to understand the cellular and molecular mechanisms leading to t...

journal_title：Journal of biomedical optics

authors： Benzina O,Szabo V,Lucas O,Saab MB,Cloitre T,Scamps F,Gergely C,Martin M

更新日期：2013-10-01 00:00:00

abstract：:The essential feature of the confocal laser scanning microscope (cLSM) is the generation of optical sections by the removal of out-of-focus light. About ten years ago, structured illumination microscopy (SIM) was introduced as an alternative method for obtaining optical sections from biological specimens. Here we comp...

journal_title：Journal of biomedical optics

authors： Weigel A,Schild D,Zeug A

更新日期：2009-01-01 00:00:00

abstract：:We compared light-induced fluorescence (LIF) to nominal injected drug dose for predicting the depth of necrosis response to photodynamic therapy (PDT) in a murine tumor model. Mice were implanted with radiation-induced fibrosarcoma (RIF) and were injected with 0, 5, or 10 mg/kg Photofrin. 630-nm light (30 J/cm(2), 75 ...

journal_title：Journal of biomedical optics

authors： Cheung R,Solonenko M,Busch TM,Del Piero F,Putt ME,Hahn SM,Yodh AG

更新日期：2003-04-01 00:00:00

abstract：:Intercellular heterogeneity is a key factor in a variety of core cellular processes including proliferation, stimulus response, carcinogenesis, and drug resistance. However, cell-to-cell variability studies at the single-cell level have been hampered by the lack of enabling experimental techniques. We present a measur...

journal_title：Journal of biomedical optics

authors： Kelbauskas L,Ashili SP,Houkal J,Smith D,Mohammadreza A,Lee KB,Forrester J,Kumar A,Anis YH,Paulson TG,Youngbull CA,Tian Y,Holl MR,Johnson RH,Meldrum DR

更新日期：2012-03-01 00:00:00

abstract：:Planar glass waveguides with a specific number of modes were fabricated by Ag(+)-Na(+) exchange in Schott SG11 glass. The effective refractive indices were determined using m-line spectroscopy in both s- and p-polarization. By using the reversed Wentzel-Kramers-Brillouin approximation, the index profiles were describe...

journal_title：Journal of biomedical optics

authors： Hassanzadeh A,Nitsche M,Armstrong S,Nabavi N,Harrison R,Dixon SJ,Langbein U,Mittler S

更新日期：2010-05-01 00:00:00