Abstract:
:Porcine epidemic diarrhea virus (PEDV) causes a devastating enteric disease with acute diarrhea, dehydration and significant mortality in swine, thereby incurring heavy economic losses in Korea. Spike (S) glycoprotein has been suggested as an important determinant for PEDV biological properties. In this study, the nucleotide and deduced amino acid sequences of the partial S glycoprotein genes of Korean PEDV isolates, including epitope region that is capable of inducing PEDV-neutralizing antibodies, were determined. The partial S glycoprotein genes were amplified by RT-PCR, cloned, sequenced, and compared with each other as well as with reference PEDV strains. By phylogenetic analysis, the Korean PEDV isolates were divided into three groups (G1, G2, G3), which had three subgroups (G1-1, G1-2, G1-3). Group1 (G1) Korean PEDV isolates were highly homologous to CV777, Br1/87, JS-2004-2, KPED-9, P-5V, SM98-1, parent DR13, and attenuated DR13, group2 (G2) Korean PEDV isolates were highly homologous to Spk1, and group3 (G3) was Chinju99 at the nucleotide and deduced amino acid sequence levels. In addition, the G1 Korean PEDV isolates didn't had several specific nucleotides and amino acids which were found in the G2 and G3 Korean PEDV isolates, and especially the G1-1 Korean PEDV isolates had specific nucleotides and amino acids which were not found in the G1-2, G1-3, G2, and G3 Korean PEDV isolates. It was suggested that many Korean PEDV isolates are closely related to the G1 including CV777, Br1/87, JS-2004-2, KPED-9, P-5 V, SM98-1, parent DR13, and attenuated DR13 rather than to the G2 and G3 including Spk1 and Chinju99, and notably more prevalent PEDVs isolated in Korea are especially close to the Chinese PEDV strain JS-2004-2 rather than Korean PEDV strains Spk1, Chinju99, KPED-9, SM98-1, parent DR13, and attenuated DR13.
journal_name
Virus Genesjournal_title
Virus genesauthors
Park SJ,Moon HJ,Yang JS,Lee CS,Song DS,Kang BK,Park BKdoi
10.1007/s11262-007-0096-xsubject
Has Abstractpub_date
2007-10-01 00:00:00pages
321-32issue
2eissn
0920-8569issn
1572-994Xjournal_volume
35pub_type
杂志文章相关文献
VIRUS GENES文献大全abstract::Ramu stunt disease of sugarcane was first reported in Papua New Guinea in the mid 1980s. The disease can reduce sugarcane yields significantly and causes severe stunting and mortality in highly susceptible cultivars. The causal agent of Ramu stunt has been investigated but its characterization has not been completed. ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-015-1279-5
更新日期:2016-02-01 00:00:00
abstract::Six recombinants of New York Board of Health (NYBH) vaccinia virus containing cDNA for Challenge Virus Standard (CVS) rabiesvirus glycoprotein (G) were produced by directing gene insertion into the vaccinia thymidine kinase (TK) locus. To regulate expression of G the promoter P7.5 (functions at early and late times po...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00125682
更新日期:1987-11-01 00:00:00
abstract::In this study, we report the discovery and molecular characterization of a novel mycovirus, Nigrospora oryzae fusarivirus 1 (NoFV1) isolated from the rice-infecting fungus Nigrospora oryzae. Excluding a poly (A) tail, the genome of the virus is 7004 nucleotide (nt) long containing three putative nonoverlapping open re...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1371-5
更新日期:2016-12-01 00:00:00
abstract::Partial genome segments of a begomovirus were previously amplified from Wissadula amplissima exhibiting yellow-mosaic and leaf-curl symptoms in the parish of St. Thomas, Jamaica and this isolate assigned to a tentative begomovirus species, Wissadula golden mosaic St. Thomas virus. To clone the complete genome of this ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0401-y
更新日期:2009-12-01 00:00:00
abstract::One hundred and twenty three cucurbit samples with one or more symptoms of leaf mosaic, leaf distortion, fruit mosaic, stunting, mottling and yellowing were collected from several locations in the northwest region of Iran. Screening by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with a cucum...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-005-6912-2
更新日期:2006-06-01 00:00:00
abstract::Tobacco bushy top disease is caused by tobacco bushy top virus (TBTV, a member of the genus Umbravirus) which is dependent on tobacco vein-distorting virus (TVDV) to act as a helper virus encapsidating TBTV and enabling its transmission by aphids. Isometric virions from diseased tobacco plants were purified and diseas...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-010-0524-1
更新日期:2010-12-01 00:00:00
abstract::The genomes (188 kbp) of the prototype Molluscum contagiosum virus type 1 (MCV-1) and a variant strain (MCV-1v) were characterized by construction of the physical maps of the viral DNA for the restriction enzymes BamHI, ClaI, EcoRI, and HindIII using a defined gene library harboring the DNA sequences of the MCV-1 geno...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00125128
更新日期:1989-11-01 00:00:00
abstract::One of the consequences of equine herpesvirus 1 (EHV-1) infection in the natural host is a neurological disease that can lead to paralysis. The pathology associated with EHV-1-induced neurological disease includes vasculitis of the small blood vessels within the central nervous system and subsequent damage to the surr...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/B:VIRU.0000032785.19420.14
更新日期:2004-08-01 00:00:00
abstract::The complete genomic sequence of a sheep lentivirus isolate that presents a slow/low phenotype in vitro has been determined. The virus, designated P1OLV, was isolated from lung cells of a naturally infected sheep in Portugal. Three overlapping DNA fragments amplified by PCR, and encompassing the entire viral genome we...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/B:VIRU.0000036380.01957.37
更新日期:2004-10-01 00:00:00
abstract::The nucleotide sequence of two cloned restriction fragments encompassing the granulin genes from the granuloviruses of the potato tuber moth, Phthorimaea operculella, PhopGV, and the Egyptian cotton leaf worm, Spodoptera littoralis, SpliGV, have been determined. Although both viruses are able to infect the same Ph. op...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008179228236
更新日期:2000-10-01 00:00:00
abstract::Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively ident...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0345-2
更新日期:2009-06-01 00:00:00
abstract::Wild-type measles virus (wtMeV) adapted well to cotton rat lung (CRL) cells after serial passages. In order to evaluate the contributions of the individual genes of wtMeV for adaptation, whole genome sequences of the adapted and original viruses were determined and analyzed. The results showed that there were two muta...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0408-4
更新日期:2009-12-01 00:00:00
abstract::Comparison of the genomic organization of variola and vaccinia viruses has been carried out. Molecular factors of virulence of these viruses is the focus of this review. Possible roles of the genes of soluble cytokine receptors, complement control proteins, factors of virus replication, and dissemination in vivo for v...
journal_title:Virus genes
pub_type: 杂志文章,评审
doi:10.1007/BF01724297
更新日期:1995-01-01 00:00:00
abstract::Jaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary adenocarcinoma (OPA), a contagious lung cancer in sheep. Previous studies have shown that the JSRV envelope protein (Env) functions as an oncogene, in that it can morphologically transform rodent fibroblast and epithelial cell lines. To obtai...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-006-0031-6
更新日期:2007-08-01 00:00:00
abstract::Based on the genetic analysis, novel, naturally occurring recombinant norovirus strains were identified in Japan. Breakpoint analysis of recombinant norovirus showed that the recombination site was at the open reading frame ORF1/ORF2 overlap. ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-010-0459-6
更新日期:2010-06-01 00:00:00
abstract::Statistically significant sequence similarity was revealed between the gene A32 product of vaccinia virus (VV), gene I products (gpI) of filamentous single-stranded DNA bacteriophages, and IVa2 gene products of adenoviruses. Four conserved sequence motifs were delineated, the two N-proximal of which correspond to the ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01702351
更新日期:1993-02-01 00:00:00
abstract::Currently, virus-based vectors, namely derivatives of the adenovirus, are frequently used in a wide variety of ex vivo or local gene therapeutic applications. However, the efficacy of virus-based vectors in systemic applications is presently still extremely limited. Complex interactions of the various vector types wit...
journal_title:Virus genes
pub_type: 杂志文章,评审
doi:10.1007/s11262-017-1498-z
更新日期:2017-10-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) is a causative agent of adult T-cell leukemia. HTLV-1 Tax1 transforming protein interacts with several PDZ domain-containing proteins, and the interaction is associated with the transforming activities of Tax1 as well as persistent HTLV-1 infection. In this study, we show th...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0259-4
更新日期:2008-10-01 00:00:00
abstract::The varicella zoster virus (VZV) ORF4 protein, one of immediate-early genes protein, is associated with the tegument in purified virions. ORF4 protein functions at both transcriptional and post-transcriptional levels, present during different phase of whole VZV life cycle. ORF4 protein acts as a nucleocytoplasm shuttl...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-1006-z
更新日期:2014-04-01 00:00:00
abstract::H1 influenza A viruses that were distinct from the classical swine H1 lineage were identified in pigs in Canada in 2003–2004; antigenic and genetic characterization identified the hemagglutinin (HA) as human H1 lineage. The viruses identified in Canadian pigs were human lineage in entirety or double (human–swine) reas...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0386-6
更新日期:2009-10-01 00:00:00
abstract::Caliciviruses are nonenveloped with a polyadenylated genome of approximately 7.6 kb and a single capsid protein. The "RNA Fold" computer program was used to analyze 3'-terminal noncoding sequences of five feline calicivirus (FCV), rabbit hemorrhagic disease virus (RHDV), and two San Miguel sea lion virus (SMSV) isolat...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01704518
更新日期:1994-07-01 00:00:00
abstract::Infectious full-length cDNA clones from the genomic RNAs of a subgroup II cucumber mosaic cucumovirus strain (Trk7) were obtained. Sequence analysis of the whole genome revealed strong homology (99%) to the genome of Q-CMV, the only subgroup II strain whose entire genomic nucleotide sequence had been available in the ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008016202128
更新日期:1999-01-01 00:00:00
abstract::Hepatitis E virus (HEV) has emerged as an important cause of epidemic and sporadic acute viral hepatitis worldwide, which is a major public health challenge. A better understanding of the interaction between the virus and the host cell would be very helpful for its therapy. Swine HEV (SHEV) open reading frame 3 (ORF3)...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0912-4
更新日期:2013-08-01 00:00:00
abstract::Avian reovirus infection causes considerable economic loss to the commercial poultry industry. Live-attenuated vaccine strain S1133 (v-S1133, derived from parent strain S1133) is considered the safest and most effective vaccine and is currently used worldwide. To identify the genes responsible for its attenuation, DNA...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-1029-5
更新日期:2014-04-01 00:00:00
abstract::Coxsackieviruses are important human pathogens that induce myocarditis and pancreatitis. However, there are no vaccines or therapeutic reagents for their clinical treatment. Although RNA interference (RNAi)-based approaches to the prevention of viral production have been developed recently, limitations to the in vivo ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-007-0192-y
更新日期:2008-02-01 00:00:00
abstract::The nucleic acid and deduced amino acid sequence of the fibre gene of the HNF-61 strain of porcine adenovirus serotype 5 (PAdV-5) was determined and compared to that of the HNF-70 strain of the same serotype (Nagy et al., J Gen Virol 82, 525-529, 2001) and also to adenovirus fibre genes from the genera Mastadenovirus ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1014580802250
更新日期:2002-03-01 00:00:00
abstract::Molecular characterization of Bovine leukemia virus (BLV) isolates from Brazil using the env gene sequences revealed a high conservation of this gene. In most cases the substitutions corresponded to silent transitions. In addition, cystein residues, potential glycosylation sites, neutralization domains and other criti...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-006-0011-x
更新日期:2007-06-01 00:00:00
abstract::The intraspecies variability of capsid proteins of five viruses of the genus Comovirus was established. Inclusion of both capsid proteins to the sequence analysis reduces some uncertainties about species/strains demarcation criteria in the Comovirus genus. New approach is proposed for discrimination of Turnip ringspot...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0443-1
更新日期:2010-04-01 00:00:00
abstract::The near-complete (99.7 %) genome sequence of a novel Russian Plum pox virus (PPV) isolate Pk, belonging to the strain Winona (W), has been determined by 454 pyrosequencing with the exception of the thirty-one 5'-terminal nucleotides. This region was amplified using 5'RACE kit and sequenced by the Sanger method. Genom...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0946-7
更新日期:2013-10-01 00:00:00
abstract::We have extended our studies using the mouse lymphoid cell system to determine if polyomavirus (Py) cell type specificity is a consequence of the simple presence of either the A and B enhancers, or if specificity results from specific combinations or arrangements of the individual enhancer elements. Mouse fibroblasts ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00125683
更新日期:1987-11-01 00:00:00