Detection, differentiation and phylogenetic analysis of cucumber mosaic virus isolates from cucurbits in the northwest region of Iran.


:One hundred and twenty three cucurbit samples with one or more symptoms of leaf mosaic, leaf distortion, fruit mosaic, stunting, mottling and yellowing were collected from several locations in the northwest region of Iran. Screening by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with a cucumber mosaic virus (CMV) polyclonal antibody, produced positive reactions from 13 samples. However, none of these positive samples reacted with a CMV subgroup-II (S-II)-specific monoclonal antibody in a triple antibody sandwich (TAS)-ELSIA. When total RNA from the CMV-infected samples was subjected to reverse transcription polymerase chain reaction (RT-PCR) with a pair of primers corresponding to the flanking regions of the virus coat protein (CP) gene, an expected DNA fragment of about 872 bp was amplified from 10 of the 13 isolates. This fragment covered the CP open reading frame (ORF) plus 92 and 123 bp of the 5' and 3' flanking regions, respectively. Restriction analysis with MspI (HpaII) was done on 9 of the PCR products and revealed a previously described CMV subgroup I (S-I) specific profile (537 and 335 bp fragments) for the isolates B13, B23, B5, SH5, SH17, S342 and S337, and an additional fragment, suggestive of combined profiles, was present for B13, SH5 and S342. Two other isolates, SH12 and B7 had a CMV S-II MspI profile (four visible fragments and a predicted non-visible 28-bp fragment on 2% agarose). Also, BsuRI (HaeIII) did not cut the PCR products characteristic of the CMV S-I specific MspI profile, whereas for the S-II isolates, BsuRI gave two fragments with sizes of approximately 559 and 313 bp. Nucleotide (nt) sequences of clones from the isolates B13, B23, SH5, SH17, S337 and SH12 were determined and aligned with those of previously published CMV strains and isolates. Consensus parsimonious trees constructed on the basis of the whole amplified region (841 nt excluding the primer sequences), CP ORF (nt or deduced amino acid data), or either of the flanking regions confirmed the RFLP data so that B13, B23, S337, SH5 and SH17 were placed in the CMV S-IA subclade, and SH12 in the S-II Clade. These analyses showed that both CMV S-I and S-II variants occur in the northwest region of Iran although S-I variants appeared to have a higher incidence.


Virus Genes


Virus genes


Bashir NS,Kalhor MR,Zarghani SN




Has Abstract


2006-06-01 00:00:00












  • Computer simulations to predict the availability of peptides with known HLA class I motifs generated by proteolysis of dengue fever virus (DFV) type 1 structural and nonstructural proteins in infected cells.

    abstract::Cytotoxic T cells that recognize dengue fever viral (DFV) peptides were reported. To predict the cleavage pattern of DFV proteins by cytoplasmic proteasomes into nonapeptides with motifs fitting known HLA class I molecules, the computer program "Findpatterns" was used. In this study the combined amino acid motifs for ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Becker Y

    更新日期:1995-01-01 00:00:00

  • Analysis of a chitinase from EpapGV, a fast killing betabaculovirus.

    abstract::The main function of baculoviral chitinase protein (V-CHIA) is to promote the final liquefaction of infected host larvae, facilitating the dispersion of occlusion bodies (OBs) in the environment. In this study, a v-chiA from Epinotia aporema Granulovirus (EpapGV) was identified and characterized. The 1,713 base pairs ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Salvador R,Ferrelli ML,Sciocco-Cap A,Romanowski V

    更新日期:2014-04-01 00:00:00

  • Expression of the X protein of hepatitis B virus in insect cells using recombinant baculoviruses.

    abstract::The baculovirus system was used to express the X protein of human hepatitis B virus (HBV). The X open reading frames (X ORFs) from cloned viral DNA of the HBV subtypes ayw and adr were introduced into the genome of Autographa californica nuclear polyhedrosis virus (AcNPV). The HBV-DNA of subtype adr derived from a hep...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Klein R,Schröder CH,Zentgraf H

    更新日期:1991-04-01 00:00:00

  • Complete genome sequence of a sapovirus from a child in Zhejiang, China.

    abstract::Although Sapovirus (Caliciviridae) has been accepted as one of the causes of acute gastroenteritis worldwide, little is known about the genetic characteristics of the whole genome of sapoviruses in China, especially those that infect humans. Here we report the complete genome sequence of a sapovirus strain, Human/Zhej...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Zhou X,Sun Y,Shang X,Gao J,Zhao X,Shuai H,Zhang R,Zhang Y

    更新日期:2016-10-01 00:00:00

  • Nucleotide sequence of the genome segment encoding nonstructural protein NS1 of bluetongue virus serotype 20 from Australia.

    abstract::The nucleotide sequence of the genome segment (S6) encoding the nonstructural protein NS1 of an Australian isolate of bluetongue virus serotype 20 (BTV 20) has been determined from a series of overlapping cDNA clones synthesized using two terminal 15-mer oligonucleotides as primers. The gene consists of 1769 nucleotid...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Cowley JA

    更新日期:1992-11-01 00:00:00

  • Clustering of Argentinean tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene.

    abstract::The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were mad...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Dewey R,Semorille L,Crisci J,Grau O

    更新日期:1996-01-01 00:00:00

  • Phylogenetic analysis of hop and grapevine isolates of hop stunt viroid supports a grapevine origin for hop stunt disease.

    abstract::We have examined sequence variability among nine isolates of hop stunt viroid (HSVd) collected from hop gardens in Tohoku district in Japan, the only area in the world where hop stunt disease is endemic. Six different consensus and one-consensus sequences as well as 12 sequence variants were detected in the nine HSVd-...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Sano T,Mimura R,Ohshima K

    更新日期:2001-01-01 00:00:00

  • The DI-DII linker of human parainfluenza virus type 3 fusion protein is critical for the virus.

    abstract::Human parainfluenza virus type 3 (HPIV3) causes the majority of childhood viral pneumonia around the world. Fusing the viral and target cell membranes is crucial for its entry into target cells, and the fusion process requires the concerted actions of two viral glycoproteins: hemagglutinin-neuraminidase (HN) and fusio...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Liu Y,Chi M,Wen H,Zhao L,Song Y,Liu N,Chi L,Wang Z

    更新日期:2020-02-01 00:00:00

  • A bioinformatics pipeline to search functional motifs within whole-proteome data: a case study of poxviruses.

    abstract::Proteins harbor domains or short linear motifs, which facilitate their functions and interactions. Finding functional motifs in protein sequences could predict the putative cellular roles or characteristics of hypothetical proteins. In this study, we present Shetti-Motif, which is an interactive tool to (i) map UniPro...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Sobhy H

    更新日期:2017-04-01 00:00:00

  • Expression of VP2 gene protein of infectious bursal disease virus detected in Korea.

    abstract::The VP2 gene DNA (1.4 kb in approximate) of a very virulent infectious bursal disease virus (vvIBDV) Chinju strain detected in Chinju, Korea was cloned into the bacmid, a baculovirus shuttle vector, through transposition of the gene from initially cloned pFastBacHTa plasmid, a baculovirus expression vector, and was su...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Kim TK,Yeo SG

    更新日期:2003-08-01 00:00:00

  • A short insert in the leader sequence of RNA 3L, a long variant of Alfalfa mosaic virus RNA3, introduces two unidentified reading frames.

    abstract::N20-RNA 3L, a large form of RNA 3 associated with Alfalfa mosaic virus (AMV) strain N20 comprises 2281 nt and has approximately 97% overall sequence similarity to the longest previously described RNA 3 of AMV strain YSMV (YSMV-RNA 3; 2188 nt). Compared with YSMV-RNA 3, N20-RNA 3L contains an additional 97 nt in the 5'...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Jayasena KW,Randles JW

    更新日期:2004-12-01 00:00:00

  • Respiratory syncytial virus (RSV) evades the human adaptive immune system by skewing the Th1/Th2 cytokine balance toward increased levels of Th2 cytokines and IgE, markers of allergy--a review.

    abstract::Infection of infants in their first year of life, children and elderly people with the respiratory syncytial virus (RSV) endangers the life of the patient. An attempt to develop a formalin-inactivated RSV (FI-RSV) vaccine during the 1960s resulted in an aggravated infection in immunized children, leading to hospitaliz...

    journal_title:Virus genes

    pub_type: 杂志文章,评审


    authors: Becker Y

    更新日期:2006-10-01 00:00:00

  • Identification and characterization of a novel Tritimovirus species isolated from wild Trisetum flavescens L., family Poaceae.

    abstract::Yellow oat-grass plants (Trisetum flavescens L.) with mild mosaic and pronounced dwarfing symptoms were observed at different locations in the Czech Republic. Electron microscope observations of symptomatic plants revealed the presence of filamentous particles and inclusion bodies characteristic of the family Potyviri...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Hassan M,Sirlova L,Jokes M,Vacke J

    更新日期:2009-08-01 00:00:00

  • Nucleotide sequence of the nucleoprotein gene of the RC.HL strain of rabies virus, a seed strain used for animal vaccine production in Japan.

    abstract::By using a phage vector (lambda ZAP II) and the mRNA extracted from IMR-32 cells infected with the RC.HL strain of rabies virus, we constructed a cDNA library from which four nucleoprotein (N)-specific cDNA clones were obtained by Southern blot hybridization. These clones contained a cDNA insert of about 1.4 kb, in wh...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Goto H,Minamoto N,Ito H,Sugiyama M,Kinjo T,Mannen K,Mifune K,Kawai A

    更新日期:1994-03-01 00:00:00

  • Complete nucleotide sequence of Nootka lupine vein-clearing virus.

    abstract::The complete genome sequence of Nootka lupine vein-clearing virus (NLVCV) was determined to be 4,172 nucleotides in length containing four open reading frames (ORFs) with a similar genetic organization of virus species in the genus Carmovirus, family Tombusviridae. The order and gene product size, starting from the 5'...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Robertson NL,Côté F,Paré C,Leblanc E,Bergeron MG,Leclerc D

    更新日期:2007-12-01 00:00:00

  • Polymerase chain-reaction-mediated cloning and expression of the coat protein gene of potato virus Y in Escherichia coli.

    abstract::Complementary DNAs (cDNAs) to the RNA genome of a necrotic strain of potato virus Y in Japan (Hokkaido Univ. isolate of PVY-T:PVY-TH) were synthesized and cloned into a plasmid pBR322. About 4.3 kbp of the cDNA sequence containing the 3'-poly(A) tract of PVY-TH was inserted into a recombinant plasmid pBRYT88. The coat...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Hataya T,Sano T,Ohshima K,Shikata E

    更新日期:1990-12-01 00:00:00

  • Genetic diversity of Apodemus agrarius-borne hantaan virus in Korea.

    abstract::Hantaan (HTN) virus, the etiologic agent of clinically severe hemorrhagic fever with renal syndrome (HFRS), was first isolated in 1976 from lung tissues of striped field mice (Apodemus agrarius) captured in Songnae-ri, Kyungki-do, Korea. To clarify the genetic diversity and phylogenetic relationship among Korean strai...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Song JW,Baek LJ,Kim SH,Kho EY,Kim JH,Yanagihara R,Song KJ

    更新日期:2000-10-01 00:00:00

  • Molecular characterization of a new Tospovirus infecting soybean.

    abstract::A new, widespread disease was recently observed in soybean in the United States. The disease, named Soybean vein necrosis, is manifested by intraveinal chlorosis and necrosis, and has been found in almost all of the 50 fields visited over a period of 3 years in the midwest and midsouth part of the United States. A vir...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Zhou J,Kantartzi SK,Wen RH,Newman M,Hajimorad MR,Rupe JC,Tzanetakis IE

    更新日期:2011-10-01 00:00:00

  • Complete genome sequence of a novel Plum pox virus strain W isolate determined by 454 pyrosequencing.

    abstract::The near-complete (99.7 %) genome sequence of a novel Russian Plum pox virus (PPV) isolate Pk, belonging to the strain Winona (W), has been determined by 454 pyrosequencing with the exception of the thirty-one 5'-terminal nucleotides. This region was amplified using 5'RACE kit and sequenced by the Sanger method. Genom...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Sheveleva A,Kudryavtseva A,Speranskaya A,Belenikin M,Melnikova N,Chirkov S

    更新日期:2013-10-01 00:00:00

  • hycu-hr6, A large homologous region of the Hyphantria cunea nucleopolyhedrovirus genome, as a powerful and versatile enhancer in insect expression systems.

    abstract::We previously identified a large homologous region (hr), hycu-hr6, in the genome of the Hyphantria cunea nucleopolyhedrovirus (HycuNPV) and suggested that hycu-hr6 was the largest baculovirus promoter enhancer hr identified so far. In this study, we examined the enhancement activity of hycu-hr6 against two promoters f...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Felipe Alves CA,Ishikawa H,Ikeda M,Kobayashi M

    更新日期:2009-12-01 00:00:00

  • Human immunodeficiency virus type-1 Tat protein induces secretory leukocyte protease inhibitor expression in African green monkey but not human cells.

    abstract::African monkeys are resistant to HIV-1 infection due to intrinsic restriction mechanisms found in their cells. However, although they can be infected by monkey-adapted modified HIV-1 particles that are designed to overcome known restriction factors, virus numbers drop to undetectable levels in immunocompetent animals....

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Özdemir S,Şengez B,Arslanoğlu A

    更新日期:2020-04-01 00:00:00

  • Phylodynamic analysis of two amino acid substitutions in the hemagglutinin protein of canine distemper virus strains detected in fur-bearing animals in China.

    abstract::Canine distemper virus (CDV) causes a highly contagious disease in a wide range of carnivores. The hemagglutinin (H) protein of viruses shows the highest variability and plays an important role in modulation of viral antigenicity, virulence, and receptor recognition. Since 2012, canine distemper (CD) outbreaks in fur-...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Tao R,Ba H,Chen J,Liu M,Pan H,Li X,Wang Q,Zhao J

    更新日期:2020-02-01 00:00:00

  • Simultaneous analysis of the bidirectional African cassava mosaic virus promoter activity using two different luciferase genes.

    abstract::The expression of geminivirus genes is controlled by bidirectional promoters which are located in the large intergenic region of the circular DNA genomes and specifically regulated by virus encoded proteins. In order to study the simultaneous regulation of both orientations of the DNA A and DNA B promoters of African ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Frey PM,Schärer-Hernández NG,Fütterer J,Potrykus I,Puonti-Kaerlas J

    更新日期:2001-03-01 00:00:00

  • Role of SH3b binding domain in a natural deletion mutant of Kayvirus endolysin LysF1 with a broad range of lytic activity.

    abstract::The spontaneous host-range mutants 812F1 and K1/420 are derived from polyvalent phage 812 that is almost identical to phage K, belonging to family Myoviridae and genus Kayvirus. Phage K1/420 is used for the phage therapy of staphylococcal infections. Endolysin of these mutants designated LysF1, consisting of an N-term...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Benešík M,Nováček J,Janda L,Dopitová R,Pernisová M,Melková K,Tišáková L,Doškař J,Žídek L,Hejátko J,Pantůček R

    更新日期:2018-02-01 00:00:00

  • Nuclear localization signals of varicella zoster virus ORF4.

    abstract::The varicella zoster virus (VZV) ORF4 protein, one of immediate-early genes protein, is associated with the tegument in purified virions. ORF4 protein functions at both transcriptional and post-transcriptional levels, present during different phase of whole VZV life cycle. ORF4 protein acts as a nucleocytoplasm shuttl...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Huang Y,Zhang J,Halawa MA,Yao S

    更新日期:2014-04-01 00:00:00

  • Complete genomic characterization of a porcine reproductive and respiratory syndrome virus isolate in Xinjiang province of China.

    abstract::Porcine reproductive and respiratory syndrome (PRRS) is a swine disease of major economic importance that causes reproductive and respiratory problems in pigs. In the present study, one strain of porcine reproductive and respiratory syndrome virus (PRRSV) was isolated in Xinjiang province, Northwest China, designated ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Xia J,Chen S,Huang J,Ma W,Du W,Yang X,Liu Y,Yan G

    更新日期:2015-02-01 00:00:00

  • A live gI/gE-deleted pseudorabies virus (PRV) protects weaned piglets against lethal variant PRV challenge.

    abstract::Emerging pseudorabies virus (PRV) variant has led to frequent outbreaks of PRV infection among Bartha-K61-vaccinated swine population in Chinese swine farms and caused high mortality in pigs of all age since late 2011. Here, we generated a gE/gI-deleted PRV (rPRVXJ-delgI/gE-EGFP) based on PRV variant strain (PRV-XJ) t...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Yin Y,Xu Z,Liu X,Li P,Yang F,Zhao J,Fan Y,Sun X,Zhu L

    更新日期:2017-08-01 00:00:00

  • Comparative analysis of the large fragment of the 5' untranslated region (LF-5' UTR) of serotype A foot-and-mouth disease virus field isolates from India.

    abstract::India is endemic for foot-and-mouth disease (FMD) and in recent years a unique group within serotype A, carrying a codon deletion at an antigenically critical site in capsid protein VP3 has emerged (VP3(59)-deletion group). This tempted us to analyze the noncoding region, which is an under represented area, though cri...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Mohapatra JK,Sahu A,Barik SK,Sanyal A,Pattnaik B

    更新日期:2009-08-01 00:00:00

  • Evolution of surface and nonstructural-1 genes of influenza B viruses isolated in the Province of Québec, Canada, during the 1998-2001 period.

    abstract::After 2 minor winter seasons, influenza B viruses were predominantly isolated in the Province of Quebec, Canada, during the 2000-2001 season representing 74% of laboratory-confirmed influenza viruses. We performed an antigenic study of the hemagglutinin (HA) protein and a molecular characterization of the HA1 region, ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Abed Y,Coulthart MB,Li Y,Boivin G

    更新日期:2003-10-01 00:00:00

  • Evolution of feline immunodeficiency virus Gag proteins.

    abstract::We evaluated the predicted biochemical properties of Gag proteins from a diverse group of feline immunodeficiency viruses (FIV) to determine how different evolutionary histories of virus and host have changed or constrained these important structural proteins. Our data are based on FIV sequences derived from domestic ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Burkala E,Poss M

    更新日期:2007-10-01 00:00:00