Integrating cell-level kinetic modeling into the design of engineered protein therapeutics.

abstract：:DNA delivery of tumor antigens can activate specific immune attack on cancer cells. However, antigens may be weak, and immune capacity can be compromised. Fusion of genes encoding activating sequences to the tumor antigen sequence facilitates promotion and manipulation of effector pathways. Idiotypic determinants of B...

journal_title：Nature biotechnology

authors： Savelyeva N,Munday R,Spellerberg MB,Lomonossoff GP,Stevenson FK

更新日期：2001-08-01 00:00:00

abstract：:Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the riboso...

journal_title：Nature biotechnology

authors： Isaacs FJ,Dwyer DJ,Ding C,Pervouchine DD,Cantor CR,Collins JJ

更新日期：2004-07-01 00:00:00

abstract：:We present 1,003 reference genomes that were sequenced as part of the Genomic Encyclopedia of Bacteria and Archaea (GEBA) initiative, selected to maximize sequence coverage of phylogenetic space. These genomes double the number of existing type strains and expand their overall phylogenetic diversity by 25%. Comparativ...

journal_title：Nature biotechnology

authors： Mukherjee S,Seshadri R,Varghese NJ,Eloe-Fadrosh EA,Meier-Kolthoff JP,Göker M,Coates RC,Hadjithomas M,Pavlopoulos GA,Paez-Espino D,Yoshikuni Y,Visel A,Whitman WB,Garrity GM,Eisen JA,Hugenholtz P,Pati A,Ivanova NN,Woyke

更新日期：2017-07-01 00:00:00

abstract：:The sap-sucking insects (order Hemiptera), including aphids, planthoppers, whiteflies and stink bugs, present one of the greatest challenges for pest management in global agriculture. Insect neurotoxins offer an alternative to chemical insecticides for controlling these pests, but require delivery into the insect hemo...

journal_title：Nature biotechnology

authors： Bonning BC,Pal N,Liu S,Wang Z,Sivakumar S,Dixon PM,King GF,Miller WA

更新日期：2014-01-01 00:00:00

abstract：:MicroRNAs (miRNAs) are increasingly implicated in the regulation of metastasis. Despite their potential as targets for anti-metastatic therapy, miRNAs have only been silenced in normal tissues of rodents and nonhuman primates. Therefore, the development of effective approaches for sequence-specific inhibition of miRNA...

journal_title：Nature biotechnology

authors： Ma L,Reinhardt F,Pan E,Soutschek J,Bhat B,Marcusson EG,Teruya-Feldstein J,Bell GW,Weinberg RA

更新日期：2010-04-01 00:00:00

abstract：:A sequence-specific genomic delivery system for the correction of chromosomal mutations was designed by incorporating two different binding domains into a single-stranded oligonucleotide. A repair domain (RD) contained the native sequence of the target region. A third strand-forming domain (TFD) was designed to form a...

journal_title：Nature biotechnology

authors： Culver KW,Hsieh WT,Huyen Y,Chen V,Liu J,Khripine Y,Khorlin A

更新日期：1999-10-01 00:00:00

abstract：:Little is known about the consequences of releasing genetically engineered plants (GEP) into the environment. Using opine-producing GEP, we show that transgenic plants alter their biological environment, more precisely the root-associated bacterial populations. The alterations were both transgene-specific and target p...

journal_title：Nature biotechnology

authors： Oger P,Petit A,Dessaux Y

更新日期：1997-04-01 00:00:00

abstract：:After nearly 40 years of development, oligonucleotide therapeutics are nearing meaningful clinical productivity. One of the key advantages of oligonucleotide drugs is that their delivery and potency are derived primarily from the chemical structure of the oligonucleotide whereas their target is defined by the base seq...

journal_title：Nature biotechnology

authors： Khvorova A,Watts JK

更新日期：2017-03-01 00:00:00

abstract：:Reagentless biosensors that can directly transduce molecular recognition to optical signals should potentiate the development of sensor arrays for a wide variety of analytes. Nucleic acid aptamers that bind ligands tightly and specifically can be readily selected, but may prove difficult to adapt to biosensor applicat...

journal_title：Nature biotechnology

authors： Jhaveri S,Rajendran M,Ellington AD

更新日期：2000-12-01 00:00:00

abstract：:Effective proteome-wide strategies that distinguish the N-termini of proteins from the N-termini of their protease cleavage products would accelerate identification of the substrates of proteases with broad or unknown specificity. Our approach, named terminal amine isotopic labeling of substrates (TAILS), addresses th...

journal_title：Nature biotechnology

authors： Kleifeld O,Doucet A,auf dem Keller U,Prudova A,Schilling O,Kainthan RK,Starr AE,Foster LJ,Kizhakkedathu JN,Overall CM

更新日期：2010-03-01 00:00:00

abstract：:Standardized benchmarking approaches are required to assess the accuracy of variants called from sequence data. Although variant-calling tools and the metrics used to assess their performance continue to improve, important challenges remain. Here, as part of the Global Alliance for Genomics and Health (GA4GH), we pres...

journal_title：Nature biotechnology

authors： Krusche P,Trigg L,Boutros PC,Mason CE,De La Vega FM,Moore BL,Gonzalez-Porta M,Eberle MA,Tezak Z,Lababidi S,Truty R,Asimenos G,Funke B,Fleharty M,Chapman BA,Salit M,Zook JM,Global Alliance for Genomics and Health Benchma

更新日期：2019-05-01 00:00:00

abstract：:Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments? ...

journal_title：Nature biotechnology

authors： Lewis K,Ausubel FM

更新日期：2006-12-01 00:00:00

abstract：:Escherichia coli-based artificial chromosomes have become important tools for physical mapping and sequencing in various genome projects. The lack of a general method to modify these large bacterial clones, however, has limited their utility in functional studies. We developed a simple method to modify bacterial artif...

journal_title：Nature biotechnology

authors： Yang XW,Model P,Heintz N

更新日期：1997-09-01 00:00:00

abstract：:Reengineering the receptor footprints of adeno-associated virus (AAV) isolates may yield variants with improved properties for clinical applications. We generated a panel of synthetic AAV2 vectors by replacing a hexapeptide sequence in a previously identified heparan sulfate receptor footprint with corresponding resid...

journal_title：Nature biotechnology

authors： Asokan A,Conway JC,Phillips JL,Li C,Hegge J,Sinnott R,Yadav S,DiPrimio N,Nam HJ,Agbandje-McKenna M,McPhee S,Wolff J,Samulski RJ

更新日期：2010-01-01 00:00:00

abstract：:Enzymatic removal of blood group ABO antigens to develop universal red blood cells (RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical pract...

journal_title：Nature biotechnology

authors： Liu QP,Sulzenbacher G,Yuan H,Bennett EP,Pietz G,Saunders K,Spence J,Nudelman E,Levery SB,White T,Neveu JM,Lane WS,Bourne Y,Olsson ML,Henrissat B,Clausen H

更新日期：2007-04-01 00:00:00

abstract：:To enhance milk composition and milk processing efficiency by increasing the casein concentration in milk, we have introduced additional copies of the genes encoding bovine beta- and kappa-casein (CSN2 and CSN3, respectively) into female bovine fibroblasts. Nuclear transfer with four independent donor cell lines resul...

journal_title：Nature biotechnology

authors： Brophy B,Smolenski G,Wheeler T,Wells D,L'Huillier P,Laible G

更新日期：2003-02-01 00:00:00

abstract：:We describe tethered conformation capture (TCC), a method for genome-wide mapping of chromatin interactions. By performing ligations on solid substrates rather than in solution, TCC substantially enhances the signal-to-noise ratio, thereby facilitating a detailed analysis of interactions within and between chromosomes...

journal_title：Nature biotechnology

authors： Kalhor R,Tjong H,Jayathilaka N,Alber F,Chen L

更新日期：2011-12-25 00:00:00

abstract：:Programmable DNA cleavage using CRISPR-Cas9 enables efficient, site-specific genome engineering in single cells and whole organisms. In the research arena, versatile CRISPR-enabled genome editing has been used in various ways, such as controlling transcription, modifying epigenomes, conducting genome-wide screens and ...

journal_title：Nature biotechnology

authors： Barrangou R,Doudna JA

更新日期：2016-01-01 00:00:00

abstract：:Peptide nucleic acids (PNAs) form stable and tight complexes with complementary DNA and/or RNA and would be promising antisense reagents if their cellular delivery could be improved. We show that a 21-mer PNA, complementary to the human galanin receptor type 1 mRNA, coupled to the cellular transporter peptides, transp...

journal_title：Nature biotechnology

authors： Pooga M,Soomets U,Hällbrink M,Valkna A,Saar K,Rezaei K,Kahl U,Hao JX,Xu XJ,Wiesenfeld-Hallin Z,Hökfelt T,Bartfai T,Langel U

更新日期：1998-09-01 00:00:00

abstract：:L-Ascorbic acid (vitamin C) in fruits and vegetables is an essential component of human nutrition. Surprisingly, only limited information is available about the pathway(s) leading to its biosynthesis in plants. Here, we report the isolation and characterization of GalUR, a gene from strawberry that encodes an NADPH-de...

journal_title：Nature biotechnology

authors： Agius F,González-Lamothe R,Caballero JL,Muñoz-Blanco J,Botella MA,Valpuesta V

更新日期：2003-02-01 00:00:00

abstract：:High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequen...

journal_title：Nature biotechnology

authors： Zhang L,Miles MF,Aldape KD

更新日期：2003-07-01 00:00:00

abstract：:We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...

journal_title：Nature biotechnology

authors： Xiao L,Feng Q,Liang S,Sonne SB,Xia Z,Qiu X,Li X,Long H,Zhang J,Zhang D,Liu C,Fang Z,Chou J,Glanville J,Hao Q,Kotowska D,Colding C,Licht TR,Wu D,Yu J,Sung JJ,Liang Q,Li J,Jia H,Lan Z,Tremaroli V,Dworzyn

更新日期：2015-10-01 00:00:00

abstract：:Current tools for targeted RNA editing rely on the delivery of exogenous proteins or chemically modified guide RNAs, which may lead to aberrant effector activity, delivery barrier or immunogenicity. Here, we present an approach, called leveraging endogenous ADAR for programmable editing of RNA (LEAPER), that employs s...

journal_title：Nature biotechnology

authors： Qu L,Yi Z,Zhu S,Wang C,Cao Z,Zhou Z,Yuan P,Yu Y,Tian F,Liu Z,Bao Y,Zhao Y,Wei W

更新日期：2019-09-01 00:00:00

abstract：:RNA interference (RNAi) is a powerful method for specifically silencing gene expression in diverse cell types. RNAi is mediated by approximately 21-nucleotide small interfering RNAs (siRNAs), which are produced from larger double-stranded RNAs (dsRNAs) in vivo through the action of Dicer, an RNase III-family enzyme. T...

journal_title：Nature biotechnology

authors： Myers JW,Jones JT,Meyer T,Ferrell JE Jr

更新日期：2003-03-01 00:00:00

abstract：:Selectable marker genes are required to ensure the efficient genetic modification of crops. Economic incentives and safety concerns have prompted the development of several strategies (site-specific recombination, homologous recombination, transposition, and co-transformation) to eliminate these genes from the genome ...

journal_title：Nature biotechnology

authors： Hare PD,Chua NH

更新日期：2002-06-01 00:00:00

abstract：:The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...

journal_title：Nature biotechnology

authors： Giordano-Coltart J,Calkins CW

更新日期：2008-02-01 00:00:00

abstract：:Field tests of corn co-expressing two new delta-endotoxins from Bacillus thuringiensis (Bt) have demonstrated protection from root damage by western corn rootworm (Diabrotica virgifera virgifera LeConte). The level of protection exceeds that provided by chemical insecticides. In the bacterium, these proteins form crys...

journal_title：Nature biotechnology

authors： Moellenbeck DJ,Peters ML,Bing JW,Rouse JR,Higgins LS,Sims L,Nevshemal T,Marshall L,Ellis RT,Bystrak PG,Lang BA,Stewart JL,Kouba K,Sondag V,Gustafson V,Nour K,Xu D,Swenson J,Zhang J,Czapla T,Schwab G,Jayne S,St

更新日期：2001-07-01 00:00:00

abstract：:The excitement and controversy surrounding the potential role of human embryonic stem (ES) cells in transplantation therapy have often overshadowed their potentially more important use as a basic research tool for understanding the development and function of human tissues. Human ES cells can proliferate without a kno...

journal_title：Nature biotechnology

authors： Xu RH,Chen X,Li DS,Li R,Addicks GC,Glennon C,Zwaka TP,Thomson JA

更新日期：2002-12-01 00:00:00

abstract：:The biological and physical sciences share a common interest in small structures (the definition of 'small' depends on the application, but can range from 1 nm to 1 mm). A vigorous trade across the borders of these areas of science is developing around new materials and tools (largely from the physical sciences) and n...

journal_title：Nature biotechnology

authors： Whitesides GM

更新日期：2003-10-01 00:00:00

abstract：:Isoflavones have drawn much attention because of their benefits to human health. These compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. Isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid...

journal_title：Nature biotechnology

authors： Jung W,Yu O,Lau SM,O'Keefe DP,Odell J,Fader G,McGonigle B

更新日期：2000-02-01 00:00:00