Applications of CRISPR technologies in research and beyond.

abstract：:Single-molecule long-read DNA sequencing with biological nanopores is fast and high-throughput but suffers reduced accuracy in homonucleotide stretches. We now combine the CsgG nanopore with the 35-residue N-terminal region of its extracellular interaction partner CsgF to produce a dual-constriction pore with improved...

journal_title：Nature biotechnology

authors： Van der Verren SE,Van Gerven N,Jonckheere W,Hambley R,Singh P,Kilgour J,Jordan M,Wallace EJ,Jayasinghe L,Remaut H

更新日期：2020-12-01 00:00:00

abstract：:Salicylic acid (SA), produced by plants as a signal in defense against pathogens, induces metabolic heating mediated by alternative respiration in flowers of thermogenic plants, and, when exogenously applied, increases leaf temperature in nonthermogenic plants. We have postulated that the latter phenomenon would be de...

journal_title：Nature biotechnology

authors： Chaerle L,Van Caeneghem W,Messens E,Lambers H,Van Montagu M,Van Der Straeten D

更新日期：1999-08-01 00:00:00

abstract：:Comparing diverse single-cell RNA sequencing (scRNA-seq) datasets generated by different technologies and in different laboratories remains a major challenge. Here we address the need for guidance in choosing algorithms leading to accurate biological interpretations of varied data types acquired with different platfor...

journal_title：Nature biotechnology

authors： Chen W,Zhao Y,Chen X,Yang Z,Xu X,Bi Y,Chen V,Li J,Choi H,Ernest B,Tran B,Mehta M,Kumar P,Farmer A,Mir A,Mehra UA,Li JL,Moos M Jr,Xiao W,Wang C

更新日期：2020-12-21 00:00:00

abstract：:Dietary omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) are usually derived from marine fish. Although production of both EPA and DHA has been engineered into land plants, including Arabidopsis, Camelina sativa and Brassica juncea, nei...

journal_title：Nature biotechnology

authors： Walsh TA,Bevan SA,Gachotte DJ,Larsen CM,Moskal WA,Merlo PA,Sidorenko LV,Hampton RE,Stoltz V,Pareddy D,Anthony GI,Bhaskar PB,Marri PR,Clark LM,Chen W,Adu-Peasah PS,Wensing ST,Zirkle R,Metz JG

更新日期：2016-08-01 00:00:00

abstract：:Academic researchers have regularly ignored patents on key technologies as a strategy to maneuver around patent thickets and freedom-to-operate issues, but they may be more at risk than they realize. ...

journal_title：Nature biotechnology

authors： Yancey A,Stewart CN Jr

更新日期：2007-11-01 00:00:00

abstract：:Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)-the basic constituent of the first generation monovalent human vaccine against Lyme ...

journal_title：Nature biotechnology

authors： Glenz K,Bouchon B,Stehle T,Wallich R,Simon MM,Warzecha H

更新日期：2006-01-01 00:00:00

abstract：:A major challenge for fluorescence imaging of living mammalian cells is maintaining viability following prolonged exposure to excitation illumination. We have monitored the dynamics of mitochondrial distribution in hamster embryos at frequent intervals over 24 h using two-photon microscopy (1,047 nm) while maintaining...

journal_title：Nature biotechnology

authors： Squirrell JM,Wokosin DL,White JG,Bavister BD

更新日期：1999-08-01 00:00:00

abstract：:L-Ascorbic acid (vitamin C) in fruits and vegetables is an essential component of human nutrition. Surprisingly, only limited information is available about the pathway(s) leading to its biosynthesis in plants. Here, we report the isolation and characterization of GalUR, a gene from strawberry that encodes an NADPH-de...

journal_title：Nature biotechnology

authors： Agius F,González-Lamothe R,Caballero JL,Muñoz-Blanco J,Botella MA,Valpuesta V

更新日期：2003-02-01 00:00:00

abstract：:This report describes the selection of highly efficient antibody catalysts by combining chemical selection from a synthetic library with directed in vitro protein evolution. Evolution started from a naive antibody library displayed on phage made from fully synthetic, antibody-encoding genes (the Human Combinatorial An...

journal_title：Nature biotechnology

authors： Cesaro-Tadic S,Lagos D,Honegger A,Rickard JH,Partridge LJ,Blackburn GM,Plückthun A

更新日期：2003-06-01 00:00:00

abstract：:Efficient delivery of small interfering (si)RNA to specific cell populations in vivo remains a formidable challenge to its successful therapeutic application. We show that siRNA synthetically linked to a CpG oligonucleotide agonist of toll-like receptor (TLR)9 targets and silences genes in TLR9(+) myeloid cells and B ...

journal_title：Nature biotechnology

authors： Kortylewski M,Swiderski P,Herrmann A,Wang L,Kowolik C,Kujawski M,Lee H,Scuto A,Liu Y,Yang C,Deng J,Soifer HS,Raubitschek A,Forman S,Rossi JJ,Pardoll DM,Jove R,Yu H

更新日期：2009-10-01 00:00:00

abstract：:Rice is a staple crop that has undergone substantial phenotypic and physiological changes during domestication. Here we resequenced the genomes of 40 cultivated accessions selected from the major groups of rice and 10 accessions of their wild progenitors (Oryza rufipogon and Oryza nivara) to >15 × raw data coverage. W...

journal_title：Nature biotechnology

authors： Xu X,Liu X,Ge S,Jensen JD,Hu F,Li X,Dong Y,Gutenkunst RN,Fang L,Huang L,Li J,He W,Zhang G,Zheng X,Zhang F,Li Y,Yu C,Kristiansen K,Zhang X,Wang J,Wright M,McCouch S,Nielsen R,Wang J,Wang W

更新日期：2011-12-11 00:00:00

abstract：:Colorectal cancer (CRC) is a leading cause of death in the developed world, yet facile preclinical models that mimic the natural stages of CRC progression are lacking. Through the orthotopic engraftment of colon organoids we describe a broadly usable immunocompetent CRC model that recapitulates the entire adenoma-aden...

journal_title：Nature biotechnology

authors： O'Rourke KP,Loizou E,Livshits G,Schatoff EM,Baslan T,Manchado E,Simon J,Romesser PB,Leach B,Han T,Pauli C,Beltran H,Rubin MA,Dow LE,Lowe SW

更新日期：2017-06-01 00:00:00

abstract：:CD4 functions as a major T-cell surface receptor for human immunodeficiency virus by binding the human immunodeficiency virus type 1 (HIV-1) envelope protein gp120 with relatively high affinity. We have developed constrained aromatically modified analogs of the secondary structures of the first domain of CD4 in order ...

journal_title：Nature biotechnology

authors： Zhang X,Gaubin M,Briant L,Srikantan V,Murali R,Saragovi U,Weiner D,Devaux C,Autiero M,Piatier-Tonneau D,Greene MI

更新日期：1997-02-01 00:00:00

abstract：:Systemic lupus erythematosus (SLE) is a multisystem chronic inflammatory disease of unknown etiology that affects many organs, including the kidney. The presence of multiple autoantibodies and other immunological abnormalities point to basic defects in immunoregulatory controls that normally maintain self-tolerance. T...

journal_title：Nature biotechnology

authors： Marino M,Ruvo M,De Falco S,Fassina G

更新日期：2000-07-01 00:00:00

abstract：:Analyzing patent data from 1995-2005 show the increasing importance of patenting by small and medium-sized biotech companies. ...

journal_title：Nature biotechnology

authors： Aggarwal S,Gupta V,Bagchi-Sen S

更新日期：2006-06-01 00:00:00

abstract：:Neuronal precursor cells are widespread in the forebrain ventricular/subventricular zone, and may provide a cellular substrate for brain repair. Clonal lines derived from single progenitors can become progressively less representative of their parental precursors with time and passage in vitro. We have developed an al...

journal_title：Nature biotechnology

authors： Wang S,Wu H,Jiang J,Delohery TM,Isdell F,Goldman SA

更新日期：1998-02-01 00:00:00

abstract：:Influenza A virus leads to yearly epidemics and sporadic pandemics. Present prophylactic strategies focus on egg-grown, live, attenuated influenza vaccines (LAIVs), in which attenuation is generated by conferring temperature sensitivity onto the virus. Here we describe an alternative approach to attenuating influenza ...

journal_title：Nature biotechnology

authors： Perez JT,Pham AM,Lorini MH,Chua MA,Steel J,tenOever BR

更新日期：2009-06-01 00:00:00

abstract：:High-throughput genetic screens have become essential tools for studying a wide variety of biological processes. Here we experimentally compare systems based on clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) or its transcriptionally repressive variant, CRISPR-inter...

journal_title：Nature biotechnology

authors： Evers B,Jastrzebski K,Heijmans JP,Grernrum W,Beijersbergen RL,Bernards R

更新日期：2016-06-01 00:00:00

abstract：:RNA-interference (RNAi) is a potent mechanism, conserved from plants to humans for specific silencing of genes, which holds promise for functional genomics and gene-targeted therapies. Here we show that bacteria engineered to produce a short hairpin RNA (shRNA) targeting a mammalian gene induce trans-kingdom RNAi in v...

journal_title：Nature biotechnology

authors： Xiang S,Fruehauf J,Li CJ

更新日期：2006-06-01 00:00:00

abstract：:The re-use of previously validated designs is critical to the evolution of synthetic biology from a research discipline to an engineering practice. Here we describe the Synthetic Biology Open Language (SBOL), a proposed data standard for exchanging designs within the synthetic biology community. SBOL represents synthe...

journal_title：Nature biotechnology

authors： Galdzicki M,Clancy KP,Oberortner E,Pocock M,Quinn JY,Rodriguez CA,Roehner N,Wilson ML,Adam L,Anderson JC,Bartley BA,Beal J,Chandran D,Chen J,Densmore D,Endy D,Grünberg R,Hallinan J,Hillson NJ,Johnson JD,Kuchinsky

更新日期：2014-06-01 00:00:00

abstract：:A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, huma...

journal_title：Nature biotechnology

authors： Sultan P,Schechner JS,McNiff JM,Hochman PS,Hughes CC,Lorber MI,Askenase PW,Pober JS

更新日期：1997-08-01 00:00:00

abstract：:To select for bacterial strains with enhanced phenotypes, random fragments of a whole genome, or a defined region of the genome, are cloned in a nonreplicating vector. The resulting plasmids are integrated by recombination into the homologous DNA region of the original strain. Integration gives rise to a nontandem dir...

journal_title：Nature biotechnology

authors： Mavingui P,Flores M,Romero D,Martínez-Romero E,Palacios R

更新日期：1997-06-01 00:00:00

abstract：:Correct reconstruction of macromolecular structure by cryo-electron microscopy (cryo-EM) relies on accurate determination of the orientation of single-particle images. For small (<100 kDa) DNA-binding proteins, obtaining particle images with sufficiently asymmetric features to correctly guide alignment is challenging....

journal_title：Nature biotechnology

authors： Aksel T,Yu Z,Cheng Y,Douglas SM

更新日期：2020-10-19 00:00:00

abstract：:Despite recent accomplishments, significant challenges remain before cloning can become a routine part of transgenic animal production. ...

journal_title：Nature biotechnology

authors： Dove A

更新日期：2000-10-01 00:00:00

abstract：:Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...

journal_title：Nature biotechnology

authors： Sarkizova S,Klaeger S,Le PM,Li LW,Oliveira G,Keshishian H,Hartigan CR,Zhang W,Braun DA,Ligon KL,Bachireddy P,Zervantonakis IK,Rosenbluth JM,Ouspenskaia T,Law T,Justesen S,Stevens J,Lane WJ,Eisenhaure T,Lan Zhang G,

更新日期：2020-02-01 00:00:00

abstract：:The vast selection of Drosophila mutants is an extraordinary resource for exploring molecular events underlying development and disease. We have designed and constructed an instrument that automatically separates Drosophila embryos of one genotype from a larger population of embryos, based on a fluorescent protein mar...

journal_title：Nature biotechnology

authors： Furlong EE,Profitt D,Scott MP

更新日期：2001-02-01 00:00:00

abstract：:In the version of this article initially published, Betalutin was described as directing a radioisotope to CD45-expressing B cells; in fact, it directs it to CD37-expressing B cells. The error has been corrected in the HTML and PDF versions of the article. ...

journal_title：Nature biotechnology

authors： Dolgin E

更新日期：2019-02-01 00:00:00

abstract：:Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in cult...

journal_title：Nature biotechnology

authors： Oberpenning F,Meng J,Yoo JJ,Atala A

更新日期：1999-02-01 00:00:00

abstract：:Pollen-mediated movement of transgenes from transplastomic oilseed rape (Brassica napus) into wild relatives will be avoided if chloroplasts are maternally transmitted. We assess the probability of chloroplast exchange between conventional oilseed rape and wild Brassica rapa to model the future behavior of transplasto...

journal_title：Nature biotechnology

authors： Scott SE,Wilkinson MJ

更新日期：1999-04-01 00:00:00

abstract：:The study of induced pluripotency often relies on experimental approaches that average measurements across a large population of cells, the majority of which do not become pluripotent. Here we used high-resolution, time-lapse imaging to trace the reprogramming process over 2 weeks from single mouse embryonic fibroblas...

journal_title：Nature biotechnology

authors： Smith ZD,Nachman I,Regev A,Meissner A

更新日期：2010-05-01 00:00:00