The Synthetic Biology Open Language (SBOL) provides a community standard for communicating designs in synthetic biology.

Abstract:

:The re-use of previously validated designs is critical to the evolution of synthetic biology from a research discipline to an engineering practice. Here we describe the Synthetic Biology Open Language (SBOL), a proposed data standard for exchanging designs within the synthetic biology community. SBOL represents synthetic biology designs in a community-driven, formalized format for exchange between software tools, research groups and commercial service providers. The SBOL Developers Group has implemented SBOL as an XML/RDF serialization and provides software libraries and specification documentation to help developers implement SBOL in their own software. We describe early successes, including a demonstration of the utility of SBOL for information exchange between several different software tools and repositories from both academic and industrial partners. As a community-driven standard, SBOL will be updated as synthetic biology evolves to provide specific capabilities for different aspects of the synthetic biology workflow.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Galdzicki M,Clancy KP,Oberortner E,Pocock M,Quinn JY,Rodriguez CA,Roehner N,Wilson ML,Adam L,Anderson JC,Bartley BA,Beal J,Chandran D,Chen J,Densmore D,Endy D,Grünberg R,Hallinan J,Hillson NJ,Johnson JD,Kuchinsky

doi

10.1038/nbt.2891

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

545-50

issue

6

eissn

1087-0156

issn

1546-1696

pii

nbt.2891

journal_volume

32

pub_type

杂志文章
  • Identification of co-regulated genes through Bayesian clustering of predicted regulatory binding sites.

    abstract::The identification of co-regulated genes and their transcription-factor binding sites (TFBS) are key steps toward understanding transcription regulation. In addition to effective laboratory assays, various computational approaches for the detection of TFBS in promoter regions of coexpressed genes have been developed. ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt802

    authors: Qin ZS,McCue LA,Thompson W,Mayerhofer L,Lawrence CE,Liu JS

    更新日期:2003-04-01 00:00:00

  • Recent Supreme Court decisions and licensing power.

    abstract::The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt0208-183

    authors: Giordano-Coltart J,Calkins CW

    更新日期:2008-02-01 00:00:00

  • Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown.

    abstract::A central hurdle in developing small interfering RNAs (siRNAs) as therapeutics is the inefficiency of their delivery across the plasma and endosomal membranes to the cytosol, where they interact with the RNA interference machinery. With the aim of improving endosomal release, a poorly understood and inefficient proces...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt.3298

    authors: Wittrup A,Ai A,Liu X,Hamar P,Trifonova R,Charisse K,Manoharan M,Kirchhausen T,Lieberman J

    更新日期:2015-08-01 00:00:00

  • Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays.

    abstract::We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 microm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing mic...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/76469

    authors: Brenner S,Johnson M,Bridgham J,Golda G,Lloyd DH,Johnson D,Luo S,McCurdy S,Foy M,Ewan M,Roth R,George D,Eletr S,Albrecht G,Vermaas E,Williams SR,Moon K,Burcham T,Pallas M,DuBridge RB,Kirchner J,Fearon K,Mao J,

    更新日期:2000-06-01 00:00:00

  • Systems strategies for developing industrial microbial strains.

    abstract::Industrial strain development requires system-wide engineering and optimization of cellular metabolism while considering industrially relevant fermentation and recovery processes. It can be conceptualized as several strategies, which may be implemented in an iterative fashion and in different orders. The key challenge...

    journal_title:Nature biotechnology

    pub_type: 杂志文章,评审

    doi:10.1038/nbt.3365

    authors: Lee SY,Kim HU

    更新日期:2015-10-01 00:00:00

  • Tumor refractoriness to anti-VEGF treatment is mediated by CD11b+Gr1+ myeloid cells.

    abstract::Vascular endothelial growth factor (VEGF) is an essential regulator of normal and abnormal blood vessel growth. A monoclonal antibody (mAb) that targets VEGF suppresses tumor growth in murine cancer models and human patients. We investigated cellular and molecular events that mediate refractoriness of tumors to anti-a...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt1323

    authors: Shojaei F,Wu X,Malik AK,Zhong C,Baldwin ME,Schanz S,Fuh G,Gerber HP,Ferrara N

    更新日期:2007-08-01 00:00:00

  • Alteration of Cre recombinase site specificity by substrate-linked protein evolution.

    abstract::Directed molecular evolution was applied to generate Cre recombinase variants that recognize a new DNA target sequence. Cre was adapted in a three-stage strategy to evolve recombinases to specifically recombine the new site. This complex multicycle task was made feasible by an improved directed-evolution procedure tha...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt1101-1047

    authors: Buchholz F,Stewart AF

    更新日期:2001-11-01 00:00:00

  • Production of human monoclonal antibody in eggs of chimeric chickens.

    abstract::The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and dow...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt1132

    authors: Zhu L,van de Lavoir MC,Albanese J,Beenhouwer DO,Cardarelli PM,Cuison S,Deng DF,Deshpande S,Diamond JH,Green L,Halk EL,Heyer BS,Kay RM,Kerchner A,Leighton PA,Mather CM,Morrison SL,Nikolov ZL,Passmore DB,Pradas-Monne

    更新日期:2005-09-01 00:00:00

  • Generation of Rhizobium strains with improved symbiotic properties by random DNA amplification (RDA)

    abstract::To select for bacterial strains with enhanced phenotypes, random fragments of a whole genome, or a defined region of the genome, are cloned in a nonreplicating vector. The resulting plasmids are integrated by recombination into the homologous DNA region of the original strain. Integration gives rise to a nontandem dir...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt0697-564

    authors: Mavingui P,Flores M,Romero D,Martínez-Romero E,Palacios R

    更新日期:1997-06-01 00:00:00

  • A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy.

    abstract::Dual-color fluorescence cross-correlation spectroscopy (FCCS) is a promising technique for quantifying protein-protein interactions. In this technique, two different fluorescent labels are excited and detected simultaneously within a common measurement volume. Difficulties in aligning two laser lines and emission cros...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt1207

    authors: Kogure T,Karasawa S,Araki T,Saito K,Kinjo M,Miyawaki A

    更新日期:2006-05-01 00:00:00

  • A simple method to cure established tumors by inflammatory killing of normal cells.

    abstract::We describe a simple technology used to cure an established metastatic disease. Intradermal injection of plasmid DNA encoding a transcriptionally targeted cytotoxic gene, along with hsp70, not only promoted tissue-specific, inflammatory killing of normal melanocytes, but also induced a CD8(+) T-cell-dependent, antigen...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt1007

    authors: Daniels GA,Sanchez-Perez L,Diaz RM,Kottke T,Thompson J,Lai M,Gough M,Karim M,Bushell A,Chong H,Melcher A,Harrington K,Vile RG

    更新日期:2004-09-01 00:00:00

  • A catalog of the mouse gut metagenome.

    abstract::We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt.3353

    authors: Xiao L,Feng Q,Liang S,Sonne SB,Xia Z,Qiu X,Li X,Long H,Zhang J,Zhang D,Liu C,Fang Z,Chou J,Glanville J,Hao Q,Kotowska D,Colding C,Licht TR,Wu D,Yu J,Sung JJ,Liang Q,Li J,Jia H,Lan Z,Tremaroli V,Dworzyn

    更新日期:2015-10-01 00:00:00

  • Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells.

    abstract::We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt.2052

    authors: Kim K,Zhao R,Doi A,Ng K,Unternaehrer J,Cahan P,Huo H,Loh YH,Aryee MJ,Lensch MW,Li H,Collins JJ,Feinberg AP,Daley GQ

    更新日期:2011-11-27 00:00:00

  • Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1) provides insights into genome evolution.

    abstract::Gossypium hirsutum has proven difficult to sequence owing to its complex allotetraploid (AtDt) genome. Here we produce a draft genome using 181-fold paired-end sequences assisted by fivefold BAC-to-BAC sequences and a high-resolution genetic map. In our assembly 88.5% of the 2,173-Mb scaffolds, which cover 89.6%∼96.7%...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt.3208

    authors: Li F,Fan G,Lu C,Xiao G,Zou C,Kohel RJ,Ma Z,Shang H,Ma X,Wu J,Liang X,Huang G,Percy RG,Liu K,Yang W,Chen W,Du X,Shi C,Yuan Y,Ye W,Liu X,Zhang X,Liu W,Wei H,Wei S,Huang G,Zhang X,Zhu S,Zhang H,Sun

    更新日期:2015-05-01 00:00:00

  • The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins.

    abstract::The green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its fluorescent homologs from Anthozoa corals have become invaluable tools for in vivo imaging of cells and tissues. Despite spectral and chromophore diversity, about 100 cloned members of the GFP-like protein family possess common structural...

    journal_title:Nature biotechnology

    pub_type: 杂志文章,评审

    doi:10.1038/nbt943

    authors: Verkhusha VV,Lukyanov KA

    更新日期:2004-03-01 00:00:00

  • Isotopic labeling of terminal amines in complex samples identifies protein N-termini and protease cleavage products.

    abstract::Effective proteome-wide strategies that distinguish the N-termini of proteins from the N-termini of their protease cleavage products would accelerate identification of the substrates of proteases with broad or unknown specificity. Our approach, named terminal amine isotopic labeling of substrates (TAILS), addresses th...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt.1611

    authors: Kleifeld O,Doucet A,auf dem Keller U,Prudova A,Schilling O,Kainthan RK,Starr AE,Foster LJ,Kizhakkedathu JN,Overall CM

    更新日期:2010-03-01 00:00:00

  • Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.

    abstract::CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off-target effects of individual sgRNAs can vary widely. Here, we use ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt.3437

    authors: Doench JG,Fusi N,Sullender M,Hegde M,Vaimberg EW,Donovan KF,Smith I,Tothova Z,Wilen C,Orchard R,Virgin HW,Listgarten J,Root DE

    更新日期:2016-02-01 00:00:00

  • Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells.

    abstract::Human embryonic stem cells (hESCs) have two properties of interest for the development of cell therapies: self-renewal and the potential to differentiate into all major lineages of somatic cells in the human body. Widespread clinical application of hESC-derived cells will require culture methods that are low-cost, rob...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt.1629

    authors: Melkoumian Z,Weber JL,Weber DM,Fadeev AG,Zhou Y,Dolley-Sonneville P,Yang J,Qiu L,Priest CA,Shogbon C,Martin AW,Nelson J,West P,Beltzer JP,Pal S,Brandenberger R

    更新日期:2010-06-01 00:00:00

  • Deciphering molecular circuits from genetic variation underlying transcriptional responsiveness to stimuli.

    abstract::Individual genetic variation affects gene responsiveness to stimuli, often by influencing complex molecular circuits. Here we combine genomic and intermediate-scale transcriptional profiling with computational methods to identify variants that affect the responsiveness of genes to stimuli (responsiveness quantitative ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt.2519

    authors: Gat-Viks I,Chevrier N,Wilentzik R,Eisenhaure T,Raychowdhury R,Steuerman Y,Shalek AK,Hacohen N,Amit I,Regev A

    更新日期:2013-04-01 00:00:00

  • MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification.

    abstract::Efficient analysis of very large amounts of raw data for peptide identification and protein quantification is a principal challenge in mass spectrometry (MS)-based proteomics. Here we describe MaxQuant, an integrated suite of algorithms specifically developed for high-resolution, quantitative MS data. Using correlatio...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt.1511

    authors: Cox J,Mann M

    更新日期:2008-12-01 00:00:00

  • Microarray reality checks in the context of a complex disease.

    abstract::A problem in analyzing microarray-based gene expression data is the separation of genes causally involved in a disease from innocent bystander genes, whose expression levels have been secondarily altered by primary changes elsewhere. To investigate this issue systematically in the context of a class of complex human d...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt965

    authors: Miklos GL,Maleszka R

    更新日期:2004-05-01 00:00:00

  • Phosphoramidate oligonucleotides as potent antisense molecules in cells and in vivo.

    abstract::Antisense oligonucleotides are designed to specifically hybridize to a target messenger RNA (mRNA) and interfere with the synthesis of the encoded protein. Uniformly modified oligonucleotides containing N3'-P5' phosphoramidate linkages exhibit (NP) extremely high-affinity binding to single-stranded RNA, do not induce ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/83489

    authors: Faria M,Spiller DG,Dubertret C,Nelson JS,White MR,Scherman D,Hélène C,Giovannangeli C

    更新日期:2001-01-01 00:00:00

  • Generation of oligodendroglial cells by direct lineage conversion.

    abstract::Transplantation of oligodendrocyte precursor cells (OPCs) is a promising potential therapeutic strategy for diseases affecting myelin. However, the derivation of engraftable OPCs from human pluripotent stem cells has proven difficult and primary OPCs are not readily available. Here we report the generation of induced ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt.2564

    authors: Yang N,Zuchero JB,Ahlenius H,Marro S,Ng YH,Vierbuchen T,Hawkins JS,Geissler R,Barres BA,Wernig M

    更新日期:2013-05-01 00:00:00

  • Integrating microarray-based spatial transcriptomics and single-cell RNA-seq reveals tissue architecture in pancreatic ductal adenocarcinomas.

    abstract::Single-cell RNA sequencing (scRNA-seq) enables the systematic identification of cell populations in a tissue, but characterizing their spatial organization remains challenging. We combine a microarray-based spatial transcriptomics method that reveals spatial patterns of gene expression using an array of spots, each ca...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/s41587-019-0392-8

    authors: Moncada R,Barkley D,Wagner F,Chiodin M,Devlin JC,Baron M,Hajdu CH,Simeone DM,Yanai I

    更新日期:2020-03-01 00:00:00

  • The extreme sensitivity of Mycobacterium tuberculosis to the front-line antituberculosis drug isoniazid.

    abstract::Mycobacterium tuberculosis is a natural mutant in oxyR, a close homolog of the central regulator of peroxide stress response in enteric bacteria. Inactivation of oxyR is specific for M. tuberculosis and other members of the M. tuberculosis complex. This phenomenon appears as a paradox due to the ability of this organi...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt1196-1557

    authors: Deretic V,Pagán-Ramos E,Zhang Y,Dhandayuthapani S,Via LE

    更新日期:1996-11-01 00:00:00

  • The paths around stem cell intellectual property.

    abstract::Can new approaches for deriving human stem cells circumnavigate existing patents that dominate embryonic stem cell intellectual property? ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt0406-411

    authors: Taymor KS,Scott CT,Greely HT

    更新日期:2006-04-01 00:00:00

  • Fully mobilizing host defense: building better vaccines.

    abstract::Developments in methods for identifying antigens from infectious agents and cancers has provided exciting new opportunities in prevention and treatment through vaccination. In many of these situations, however, traditional immunization techniques do not stimulate protective immunity because they fail to fully mobilize...

    journal_title:Nature biotechnology

    pub_type: 杂志文章,评审

    doi:10.1038/3469

    authors: Raychaudhuri S,Rock KL

    更新日期:1998-11-01 00:00:00

  • Encapsulated cell technology.

    abstract::The potential therapeutic applications of encapsulated cells are enormous. In the US alone, it has been estimated that nearly half-a-trillion dollars are spent each year to care for patients who suffer tissue loss or dysfunction. Over 6 million patients suffer from neurodegenerative disorders such as Alzheimer's disea...

    journal_title:Nature biotechnology

    pub_type: 杂志文章,评审

    doi:10.1038/nbt0996-1107

    authors: Lanza RP,Hayes JL,Chick WL

    更新日期:1996-09-01 00:00:00

  • Analysis of 589,306 genomes identifies individuals resilient to severe Mendelian childhood diseases.

    abstract::Genetic studies of human disease have traditionally focused on the detection of disease-causing mutations in afflicted individuals. Here we describe a complementary approach that seeks to identify healthy individuals resilient to highly penetrant forms of genetic childhood disorders. A comprehensive screen of 874 gene...

    journal_title:Nature biotechnology

    pub_type: 杂志文章

    doi:10.1038/nbt.3514

    authors: Chen R,Shi L,Hakenberg J,Naughton B,Sklar P,Zhang J,Zhou H,Tian L,Prakash O,Lemire M,Sleiman P,Cheng WY,Chen W,Shah H,Shen Y,Fromer M,Omberg L,Deardorff MA,Zackai E,Bobe JR,Levin E,Hudson TJ,Groop L,Wang J,

    更新日期:2016-05-01 00:00:00

  • DNA patenting: the end of an era?

    abstract::Debates on patenting DNA must evolve to reflect the global decline in filings and regional disparities in patenting activity. ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章,评审

    doi:10.1038/nbt0207-185

    authors: Hopkins MM,Mahdi S,Patel P,Thomas SM

    更新日期:2007-02-01 00:00:00