当前位置： SCI文献检索 > NATURE BIOTECHNOLOGY期刊下所有文献 > Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells.

Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells.

Abstract：

:Human embryonic stem cells (hESCs) have two properties of interest for the development of cell therapies: self-renewal and the potential to differentiate into all major lineages of somatic cells in the human body. Widespread clinical application of hESC-derived cells will require culture methods that are low-cost, robust, scalable and use chemically defined raw materials. Here we describe synthetic peptide-acrylate surfaces (PAS) that support self-renewal of hESCs in chemically defined, xeno-free medium. H1 and H7 hESCs were successfully maintained on PAS for over ten passages. Cell morphology and phenotypic marker expression were similar for cells cultured on PAS or Matrigel. Cells on PAS retained normal karyotype and pluripotency and were able to differentiate to functional cardiomyocytes on PAS. Finally, PAS were scaled up to large culture-vessel formats. Synthetic, xeno-free, scalable surfaces that support the self-renewal and differentiation of hESCs will be useful for both research purposes and development of cell therapies.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Melkoumian Z,Weber JL,Weber DM,Fadeev AG,Zhou Y,Dolley-Sonneville P,Yang J,Qiu L,Priest CA,Shogbon C,Martin AW,Nelson J,West P,Beltzer JP,Pal S,Brandenberger R

doi

10.1038/nbt.1629

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

606-10

issue

6

eissn

1087-0156

issn

1546-1696

pii

nbt.1629

journal_volume

28

pub_type

杂志文章

相关文献

NATURE BIOTECHNOLOGY文献大全
  • A model of molecular interactions on short oligonucleotide microarrays.

    abstract：:High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequen...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt836

    authors： Zhang L,Miles MF,Aldape KD

    更新日期：2003-07-01 00:00:00

  • A bacterial glycosidase enables mannose-6-phosphate modification and improved cellular uptake of yeast-produced recombinant human lysosomal enzymes.

    abstract：:Lysosomal storage diseases are treated with human lysosomal enzymes produced in mammalian cells. Such enzyme therapeutics contain relatively low levels of mannose-6-phosphate, which is required to target them to the lysosomes of patient cells. Here we describe a method for increasing mannose-6-phosphate modification o...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.2427

    authors： Tiels P,Baranova E,Piens K,De Visscher C,Pynaert G,Nerinckx W,Stout J,Fudalej F,Hulpiau P,Tännler S,Geysens S,Van Hecke A,Valevska A,Vervecken W,Remaut H,Callewaert N

    更新日期：2012-12-01 00:00:00

  • A biodegradable polymer as a cytokine delivery system for inducing bone formation.

    abstract：:Bone morphogenetic proteins (BMPs) that have the potential to elicit new bone in vivo have been used in a tissue-engineering approach for the repair of bone injuries and bone defects. Although it is now possible to generate large amounts of recombinant human (rh) BMPs for medical use, the major challenge remains in th...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/86715

    authors： Saito N,Okada T,Horiuchi H,Murakami N,Takahashi J,Nawata M,Ota H,Nozaki K,Takaoka K

    更新日期：2001-04-01 00:00:00

  • The pharmacological basis of anti-IgE therapy.

    abstract：:The treatment of asthma and allergic rhinitis using unique, humanized anti-IgE monoclonal antibodies with very particular binding specificities is now supported by the results of multiple phase II and III human clinical studies. The therapeutic efficacy of this approach is attributable to several pharmacological mecha...

    journal_title：Nature biotechnology

    pub_type： 杂志文章,评审

    doi：10.1038/72601

    authors： Chang TW

    更新日期：2000-02-01 00:00:00

  • Engineering dynamic pathway regulation using stress-response promoters.

    abstract：:Heterologous pathways used in metabolic engineering may produce intermediates toxic to the cell. Dynamic control of pathway enzymes could prevent the accumulation of these metabolites, but such a strategy requires sensors, which are largely unknown, that can detect and respond to the metabolite. Here we applied whole-...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.2689

    authors： Dahl RH,Zhang F,Alonso-Gutierrez J,Baidoo E,Batth TS,Redding-Johanson AM,Petzold CJ,Mukhopadhyay A,Lee TS,Adams PD,Keasling JD

    更新日期：2013-11-01 00:00:00

  • The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K.

    abstract：:Lactobacillus sakei is a psychotrophic lactic acid bacterium found naturally on fresh meat and fish. This microorganism is widely used in the manufacture of fermented meats and has biotechnological potential in biopreservation and food safety. We have explored the 1,884,661-base-pair (bp) circular chromosome of strain...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt1160

    authors： Chaillou S,Champomier-Vergès MC,Cornet M,Crutz-Le Coq AM,Dudez AM,Martin V,Beaufils S,Darbon-Rongère E,Bossy R,Loux V,Zagorec M

    更新日期：2005-12-01 00:00:00

  • Sensitive digital quantification of DNA methylation in clinical samples.

    abstract：:Analysis of abnormally methylated genes is increasingly important in basic research and in the development of cancer biomarkers. We have developed methyl-BEAMing technology to enable absolute quantification of the number of methylated molecules in a sample. Individual DNA fragments are amplified and analyzed either by...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.1559

    authors： Li M,Chen WD,Papadopoulos N,Goodman SN,Bjerregaard NC,Laurberg S,Levin B,Juhl H,Arber N,Moinova H,Durkee K,Schmidt K,He Y,Diehl F,Velculescu VE,Zhou S,Diaz LA Jr,Kinzler KW,Markowitz SD,Vogelstein B

    更新日期：2009-09-01 00:00:00

  • Genome sequence of foxtail millet (Setaria italica) provides insights into grass evolution and biofuel potential.

    abstract：:Foxtail millet (Setaria italica), a member of the Poaceae grass family, is an important food and fodder crop in arid regions and has potential for use as a C(4) biofuel. It is a model system for other biofuel grasses, including switchgrass and pearl millet. We produced a draft genome (∼423 Mb) anchored onto nine chrom...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.2195

    authors： Zhang G,Liu X,Quan Z,Cheng S,Xu X,Pan S,Xie M,Zeng P,Yue Z,Wang W,Tao Y,Bian C,Han C,Xia Q,Peng X,Cao R,Yang X,Zhan D,Hu J,Zhang Y,Li H,Li H,Li N,Wang J,Wang C,Wang R,Guo T,Cai Y,Liu C,Xiang H,

    更新日期：2012-05-13 00:00:00

  • At the heart of genetic testing.

    abstract：:Genetic testing for rare heart conditions might someday expand to more common cardiac ailments. Already there are signs testing is dramatically changing how some conditions are treated and doctors' definition of who a patient is. Stephen Strauss reports. ...

    journal_title：Nature biotechnology

    pub_type： 新闻

    doi：10.1038/nbt1010-1003

    authors： Strauss S

    更新日期：2010-10-01 00:00:00

  • An integrated mass spectrometric and computational framework for the analysis of protein interaction networks.

    abstract：:Biological systems are controlled by protein complexes that associate into dynamic protein interaction networks. We describe a strategy that analyzes protein complexes through the integration of label-free, quantitative mass spectrometry and computational analysis. By evaluating peptide intensity profiles throughout t...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt1289

    authors： Rinner O,Mueller LN,Hubálek M,Müller M,Gstaiger M,Aebersold R

    更新日期：2007-03-01 00:00:00

  • Analysis of 589,306 genomes identifies individuals resilient to severe Mendelian childhood diseases.

    abstract：:Genetic studies of human disease have traditionally focused on the detection of disease-causing mutations in afflicted individuals. Here we describe a complementary approach that seeks to identify healthy individuals resilient to highly penetrant forms of genetic childhood disorders. A comprehensive screen of 874 gene...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.3514

    authors： Chen R,Shi L,Hakenberg J,Naughton B,Sklar P,Zhang J,Zhou H,Tian L,Prakash O,Lemire M,Sleiman P,Cheng WY,Chen W,Shah H,Shen Y,Fromer M,Omberg L,Deardorff MA,Zackai E,Bobe JR,Levin E,Hudson TJ,Groop L,Wang J,

    更新日期：2016-05-01 00:00:00

  • Reengineering a receptor footprint of adeno-associated virus enables selective and systemic gene transfer to muscle.

    abstract：:Reengineering the receptor footprints of adeno-associated virus (AAV) isolates may yield variants with improved properties for clinical applications. We generated a panel of synthetic AAV2 vectors by replacing a hexapeptide sequence in a previously identified heparan sulfate receptor footprint with corresponding resid...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.1599

    authors： Asokan A,Conway JC,Phillips JL,Li C,Hegge J,Sinnott R,Yadav S,DiPrimio N,Nam HJ,Agbandje-McKenna M,McPhee S,Wolff J,Samulski RJ

    更新日期：2010-01-01 00:00:00

  • Generation of oligodendroglial cells by direct lineage conversion.

    abstract：:Transplantation of oligodendrocyte precursor cells (OPCs) is a promising potential therapeutic strategy for diseases affecting myelin. However, the derivation of engraftable OPCs from human pluripotent stem cells has proven difficult and primary OPCs are not readily available. Here we report the generation of induced ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.2564

    authors： Yang N,Zuchero JB,Ahlenius H,Marro S,Ng YH,Vierbuchen T,Hawkins JS,Geissler R,Barres BA,Wernig M

    更新日期：2013-05-01 00:00:00

  • Valuation--what you need to know.

    abstract：:The basics of determining valuation will help you to understand what criteria matter most for investors. ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：

    authors： Frei P,Leleux B

    更新日期：2004-08-01 00:00:00

  • Isotopic labeling of terminal amines in complex samples identifies protein N-termini and protease cleavage products.

    abstract：:Effective proteome-wide strategies that distinguish the N-termini of proteins from the N-termini of their protease cleavage products would accelerate identification of the substrates of proteases with broad or unknown specificity. Our approach, named terminal amine isotopic labeling of substrates (TAILS), addresses th...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.1611

    authors： Kleifeld O,Doucet A,auf dem Keller U,Prudova A,Schilling O,Kainthan RK,Starr AE,Foster LJ,Kizhakkedathu JN,Overall CM

    更新日期：2010-03-01 00:00:00

  • Identification of co-regulated genes through Bayesian clustering of predicted regulatory binding sites.

    abstract：:The identification of co-regulated genes and their transcription-factor binding sites (TFBS) are key steps toward understanding transcription regulation. In addition to effective laboratory assays, various computational approaches for the detection of TFBS in promoter regions of coexpressed genes have been developed. ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt802

    authors： Qin ZS,McCue LA,Thompson W,Mayerhofer L,Lawrence CE,Liu JS

    更新日期：2003-04-01 00:00:00

  • Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing.

    abstract：:RNA interference (RNAi) is a powerful method for specifically silencing gene expression in diverse cell types. RNAi is mediated by approximately 21-nucleotide small interfering RNAs (siRNAs), which are produced from larger double-stranded RNAs (dsRNAs) in vivo through the action of Dicer, an RNase III-family enzyme. T...

    journal_title：Nature biotechnology

    pub_type：

    doi：10.1038/nbt792

    authors： Myers JW,Jones JT,Meyer T,Ferrell JE Jr

    更新日期：2003-03-01 00:00:00

  • Milking the genome for profit.

    abstract：:Despite recent accomplishments, significant challenges remain before cloning can become a routine part of transgenic animal production. ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/80231

    authors： Dove A

    更新日期：2000-10-01 00:00:00

  • Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo.

    abstract：:Development of a cell therapy for diabetes would be greatly aided by a renewable supply of human beta-cells. Here we show that pancreatic endoderm derived from human embryonic stem (hES) cells efficiently generates glucose-responsive endocrine cells after implantation into mice. Upon glucose stimulation of the implant...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt1393

    authors： Kroon E,Martinson LA,Kadoya K,Bang AG,Kelly OG,Eliazer S,Young H,Richardson M,Smart NG,Cunningham J,Agulnick AD,D'Amour KA,Carpenter MK,Baetge EE

    更新日期：2008-04-01 00:00:00

  • The extreme sensitivity of Mycobacterium tuberculosis to the front-line antituberculosis drug isoniazid.

    abstract：:Mycobacterium tuberculosis is a natural mutant in oxyR, a close homolog of the central regulator of peroxide stress response in enteric bacteria. Inactivation of oxyR is specific for M. tuberculosis and other members of the M. tuberculosis complex. This phenomenon appears as a paradox due to the ability of this organi...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt1196-1557

    authors： Deretic V,Pagán-Ramos E,Zhang Y,Dhandayuthapani S,Via LE

    更新日期：1996-11-01 00:00:00

  • A large peptidome dataset improves HLA class I epitope prediction across most of the human population.

    abstract：:Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/s41587-019-0322-9

    authors： Sarkizova S,Klaeger S,Le PM,Li LW,Oliveira G,Keshishian H,Hartigan CR,Zhang W,Braun DA,Ligon KL,Bachireddy P,Zervantonakis IK,Rosenbluth JM,Ouspenskaia T,Law T,Justesen S,Stevens J,Lane WJ,Eisenhaure T,Lan Zhang G,

    更新日期：2020-02-01 00:00:00

  • Automated sorting of live transgenic embryos.

    abstract：:The vast selection of Drosophila mutants is an extraordinary resource for exploring molecular events underlying development and disease. We have designed and constructed an instrument that automatically separates Drosophila embryos of one genotype from a larger population of embryos, based on a fluorescent protein mar...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/84422

    authors： Furlong EE,Profitt D,Scott MP

    更新日期：2001-02-01 00:00:00

  • Identification of a cyclohexylcarbonyl CoA biosynthetic gene cluster and application in the production of doramectin.

    abstract：:The side chain of the antifungal antibiotic ansatrienin A from Streptomyces collinus contains a cyclohexanecarboxylic acid (CHC)-derived moiety. This moiety is also observed in trace amounts of omega-cyclohexyl fatty acids (typically less than 1% of total fatty acids) produced by S. collinus. Coenzyme A-activated CHC ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/79479

    authors： Cropp TA,Wilson DJ,Reynolds KA

    更新日期：2000-09-01 00:00:00

  • Mutation detection by electrocatalysis at DNA-modified electrodes.

    abstract：:Detection of mutations and damaged DNA bases is important for the early diagnosis of genetic disease. Here we describe an electrocatalytic method for the detection of single-base mismatches as well as DNA base lesions in fully hybridized duplexes, based on charge transport through DNA films. Gold electrodes modified w...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/80301

    authors： Boon EM,Ceres DM,Drummond TG,Hill MG,Barton JK

    更新日期：2000-10-01 00:00:00

  • Homology-driven genome editing in hematopoietic stem and progenitor cells using ZFN mRNA and AAV6 donors.

    abstract：:Genome editing with targeted nucleases and DNA donor templates homologous to the break site has proven challenging in human hematopoietic stem and progenitor cells (HSPCs), and particularly in the most primitive, long-term repopulating cell population. Here we report that combining electroporation of zinc finger nucle...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.3408

    authors： Wang J,Exline CM,DeClercq JJ,Llewellyn GN,Hayward SB,Li PW,Shivak DA,Surosky RT,Gregory PD,Holmes MC,Cannon PM

    更新日期：2015-12-01 00:00:00

  • Enrichment and analysis of peptide subsets using fluorous affinity tags and mass spectrometry.

    abstract：:Although mass spectrometry has become a powerful tool for the functional analysis of biological systems, complete proteome characterization cannot yet be achieved. Instead, the sheer complexity of living organisms demands fractionation of cellular extracts to enable more targeted analyses. Here, we introduce the conce...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt1076

    authors： Brittain SM,Ficarro SB,Brock A,Peters EC

    更新日期：2005-04-01 00:00:00

  • MALDI-TOF based mutation detection using tagged in vitro synthesized peptides.

    abstract：:Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) is a powerful method to quickly and accurately determine the masses of peptides. Most genetic analyses, however, begin with PCR amplification of a test sequence to generate DNA, which is more difficult than peptides to analyze by ...

    journal_title：Nature biotechnology

    pub_type：

    doi：10.1038/72013

    authors： Garvin AM,Parker KC,Haff L

    更新日期：2000-01-01 00:00:00

  • Metabolic selection of glycosylation defects in human cells.

    abstract：:Changes in glycosylation are often associated with disease progression, but the genetic and metabolic basis of these events is rarely understood in detail at a molecular level. We describe a metabolism-based approach to the selection of mutants in glycoconjugate biosynthesis that provides insight into regulatory mecha...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/89305

    authors： Yarema KJ,Goon S,Bertozzi CR

    更新日期：2001-06-01 00:00:00

  • Author Correction: Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature biotechnology

    pub_type： 已发布勘误

    doi：10.1038/s41587-019-0292-y

    authors： Qu L,Yi Z,Zhu S,Wang C,Cao Z,Zhou Z,Yuan P,Yu Y,Tian F,Liu Z,Bao Y,Zhao Y,Wei W

    更新日期：2019-11-01 00:00:00

  • Generation of higher affinity T cell receptors by antigen-driven differentiation of progenitor T cells in vitro.

    abstract：:Many promising targets for T-cell-based cancer immunotherapies are self-antigens. During thymic selection, T cells bearing T cell receptors (TCRs) with high affinity for self-antigen are eliminated. The affinity of the remaining low-avidity TCRs can be improved to increase their antitumor efficacy, but conventional sa...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.4004

    authors： Schmitt TM,Aggen DH,Ishida-Tsubota K,Ochsenreither S,Kranz DM,Greenberg PD

    更新日期：2017-12-01 00:00:00