Abstract：
:The green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its fluorescent homologs from Anthozoa corals have become invaluable tools for in vivo imaging of cells and tissues. Despite spectral and chromophore diversity, about 100 cloned members of the GFP-like protein family possess common structural, biochemical and photophysical features. Anthozoa GFP-like proteins are available in colors and properties unlike those of A. victoria GFP variants and thus provide powerful new fluorophores for molecular labeling and intracellular detection. Although Anthozoa GFP-like proteins provide some advantages over GFP, they also have certain drawbacks, such as obligate oligomerization and slow or incomplete fluorescence maturation. In the past few years, effective approaches for eliminating some of these limitations have been described. In addition, several Anthozoa GFP-like proteins have been developed into novel imaging agents, such as monomeric red and dimeric far-red fluorescent proteins, fluorescent timers and photoconvertible fluorescent labels. Future studies on the structure of this diverse set of proteins will further enhance their use in animal tissues and as intracellular biosensors.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Verkhusha VV,Lukyanov KAdoi
10.1038/nbt943subject
Has Abstractpub_date
2004-03-01 00:00:00pages
289-96issue
3eissn
1087-0156issn
1546-1696pii
nbt943journal_volume
22pub_type
杂志文章,评审abstract：:As the cost of human genome sequencing plunges and large-scale genome-phenotype studies become possible, society should do more to reward those individuals who choose to disclose their data, despite the risks. ...
journal_title：Nature biotechnology
pub_type： 评论,社论
doi：10.1038/nbt0909-777
更新日期：2009-09-01 00:00:00
abstract：:Tyrosine phosphorylation exerts a pivotal role in cell regulation processes of higher eukaryotes. Tight control of the activity of protein tyrosine kinases is crucial for ordered phosphorylation to occur. We have developed a functional screen for tyrosine kinase regulators using c-Src, the first cellular protein tyros...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0596-600
更新日期：1996-05-01 00:00:00
abstract：:Structural genomics has the ambitious goal of delivering three-dimensional structural information on a genome-wide scale. Yet only a small fraction of natural proteins are suitable for structure determination because of bottlenecks such as poor expression, aggregation, and misfolding of proteins, and difficulties in s...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt732
更新日期：2002-09-01 00:00:00
abstract：:The specificity of biological regulatory mechanisms relies on selective interactions between different proteins in different cell types and in response to different extracellular signals. We describe a bimolecular fluorescence complementation (BiFC) approach for the simultaneous visualization of multiple protein inter...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt816
更新日期：2003-05-01 00:00:00
abstract：:Genetic studies of human disease have traditionally focused on the detection of disease-causing mutations in afflicted individuals. Here we describe a complementary approach that seeks to identify healthy individuals resilient to highly penetrant forms of genetic childhood disorders. A comprehensive screen of 874 gene...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3514
更新日期：2016-05-01 00:00:00
abstract：:De novo assembly of a human genome using nanopore long-read sequences has been reported, but it used more than 150,000 CPU hours and weeks of wall-clock time. To enable rapid human genome assembly, we present Shasta, a de novo long-read assembler, and polishing algorithms named MarginPolish and HELEN. Using a single P...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0503-6
更新日期：2020-09-01 00:00:00
abstract：:We describe tethered conformation capture (TCC), a method for genome-wide mapping of chromatin interactions. By performing ligations on solid substrates rather than in solution, TCC substantially enhances the signal-to-noise ratio, thereby facilitating a detailed analysis of interactions within and between chromosomes...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2057
更新日期：2011-12-25 00:00:00
abstract：:Glycoprotein hormones are noncovalent heterodimers comprised of a common alpha subunit and a hormone-specific beta subunit. Secretion and biologic action of these hormones are dependent on the formation of the heterodimer. The human LH beta subunit is unique among the other beta subunits in that it assembles inefficie...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0797-663
更新日期：1997-07-01 00:00:00
abstract：:We describe wavelength-shifting molecular beacons, which are nucleic acid hybridization probes that fluoresce in a variety of different colors, yet are excited by a common monochromatic light source. The twin functions of absorption of energy from the excitation light and emission of that energy in the form of fluores...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/81192
更新日期：2000-11-01 00:00:00
abstract：:We constructed an immunotoxin, composed of an antibody directed against the high-affinity IgG receptor CD64 and Ricin-A, with the aim of resolving chronic inflammation through elimination of activated macrophages. In vitro, this immunotoxin proved very efficient in inducing apoptosis in activated macrophages, leaving ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/71908
更新日期：2000-01-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-020-0578-0
更新日期：2020-08-01 00:00:00
abstract：:Developments in methods for identifying antigens from infectious agents and cancers has provided exciting new opportunities in prevention and treatment through vaccination. In many of these situations, however, traditional immunization techniques do not stimulate protective immunity because they fail to fully mobilize...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
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更新日期：1998-11-01 00:00:00
abstract：:The interaction between the HSP90 chaperone and its client kinases is sensitive to the conformational status of the kinase, and stabilization of the kinase fold by small molecules strongly decreases chaperone interaction. Here we exploit this observation and assay small-molecule binding to kinases in living cells, usi...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2620
更新日期：2013-07-01 00:00:00
abstract：:Although mass spectrometry is well suited to identifying thousands of potential protein post-translational modifications (PTMs), it has historically been biased towards just a few. To measure the entire set of PTMs across diverse proteomes, software must overcome the dual challenges of covering enormous search spaces ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0067-5
更新日期：2019-04-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1211
更新日期：2006-06-01 00:00:00
abstract：:Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments? ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1206-1504
更新日期：2006-12-01 00:00:00
abstract：:Efficient analysis of very large amounts of raw data for peptide identification and protein quantification is a principal challenge in mass spectrometry (MS)-based proteomics. Here we describe MaxQuant, an integrated suite of algorithms specifically developed for high-resolution, quantitative MS data. Using correlatio...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1511
更新日期：2008-12-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0656-3
更新日期：2020-09-07 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/13684
更新日期：1999-10-01 00:00:00
abstract：:Numerous biological processes involve the recognition of a specific pattern of binding sites on a target protein or surface. Although ligands displayed by disordered scaffolds form stochastic rather than specific patterns, theoretical models predict that recognition will occur between patterns that are characterized b...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1204
更新日期：2006-05-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt986
更新日期：2004-07-01 00:00:00
abstract：:Nitrile hydratase (NHase), which catalyzes the hydration of nitriles to amides, has been used in the industrial production of acrylamide and nicotinamide. Recent studies on NHases, which are roughly classified into iron and cobalt types according to the metal involved, have clarified the photoactivation mechanism, the...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt0898-733
更新日期：1998-08-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 社论
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更新日期：2007-02-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/72601
更新日期：2000-02-01 00:00:00
abstract：:Systemic gene delivery into muscle has been a major challenge for muscular dystrophy gene therapy, with capillary blood vessels posing the principle barrier and limiting vector dissemination. Previous efforts to deliver genes into multiple muscles have relied on isolated vessel perfusion or pharmacological interventio...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1073
更新日期：2005-03-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2798
更新日期：2014-02-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-019-0167-2
更新日期：2019-07-01 00:00:00
abstract：:Pollen-mediated movement of transgenes from transplastomic oilseed rape (Brassica napus) into wild relatives will be avoided if chloroplasts are maternally transmitted. We assess the probability of chloroplast exchange between conventional oilseed rape and wild Brassica rapa to model the future behavior of transplasto...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/7952
更新日期：1999-04-01 00:00:00
abstract：:RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic and clinical research, but the determinants of targeting specificity and the extent of off-target cleavage remain insufficiently understood. Using chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq), we mapped genom...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2916
更新日期：2014-07-01 00:00:00
abstract：:Gossypium hirsutum has proven difficult to sequence owing to its complex allotetraploid (AtDt) genome. Here we produce a draft genome using 181-fold paired-end sequences assisted by fivefold BAC-to-BAC sequences and a high-resolution genetic map. In our assembly 88.5% of the 2,173-Mb scaffolds, which cover 89.6%∼96.7%...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3208
更新日期：2015-05-01 00:00:00
