Statistical pattern matching facilitates the design of polyvalent inhibitors of anthrax and cholera toxins.

abstract：:To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently form...

journal_title：Nature biotechnology

authors： Ikeno M,Grimes B,Okazaki T,Nakano M,Saitoh K,Hoshino H,McGill NI,Cooke H,Masumoto H

更新日期：1998-05-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Röst HL,Rosenberger G,Navarro P,Gillet L,Miladinović SM,Schubert OT,Wolski W,Collins BC,Malmström J,Malmström L,Aebersold R

更新日期：2020-03-01 00:00:00

abstract：:Dietary omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) are usually derived from marine fish. Although production of both EPA and DHA has been engineered into land plants, including Arabidopsis, Camelina sativa and Brassica juncea, nei...

journal_title：Nature biotechnology

authors： Walsh TA,Bevan SA,Gachotte DJ,Larsen CM,Moskal WA,Merlo PA,Sidorenko LV,Hampton RE,Stoltz V,Pareddy D,Anthony GI,Bhaskar PB,Marri PR,Clark LM,Chen W,Adu-Peasah PS,Wensing ST,Zirkle R,Metz JG

更新日期：2016-08-01 00:00:00

abstract：:We describe a simple technology used to cure an established metastatic disease. Intradermal injection of plasmid DNA encoding a transcriptionally targeted cytotoxic gene, along with hsp70, not only promoted tissue-specific, inflammatory killing of normal melanocytes, but also induced a CD8(+) T-cell-dependent, antigen...

journal_title：Nature biotechnology

authors： Daniels GA,Sanchez-Perez L,Diaz RM,Kottke T,Thompson J,Lai M,Gough M,Karim M,Bushell A,Chong H,Melcher A,Harrington K,Vile RG

更新日期：2004-09-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) is a powerful method to quickly and accurately determine the masses of peptides. Most genetic analyses, however, begin with PCR amplification of a test sequence to generate DNA, which is more difficult than peptides to analyze by ...

journal_title：Nature biotechnology

authors： Garvin AM,Parker KC,Haff L

更新日期：2000-01-01 00:00:00

abstract：:Three national patent offices have consulted on patents that cover protein three-dimensional structural data and pharmacophores, with significant implications for the biotechnology industry. ...

journal_title：Nature biotechnology

authors： Shimbo I,Nakajima R,Yokoyama S,Sumikura K

更新日期：2004-01-01 00:00:00

abstract：:The construction of synthetic cell-cell communication networks can improve our quantitative understanding of naturally occurring signaling pathways and enhance our capabilities to engineer coordinated cellular behavior in cell populations. Towards accomplishing these goals in eukaryotes, we developed and analyzed two ...

journal_title：Nature biotechnology

authors： Chen MT,Weiss R

更新日期：2005-12-01 00:00:00

abstract：:Evolution of eukaryotes is mediated by sexual recombination of parental genomes. Crossovers occur in random, but homologous, positions at a frequency that depends on DNA length. As exons occupy only 1% of the human genome and introns about 24%, by far most of the crossovers occur between exons, rather than inside. The...

journal_title：Nature biotechnology

authors： Kolkman JA,Stemmer WP

更新日期：2001-05-01 00:00:00

abstract：:Increasing the in vivo residence times of protein therapeutics could decrease their dosing frequencies. We show that genetic fusion of an unstructured recombinant polypeptide of 864 amino acids, called XTEN, to a peptide or protein provides an apparently generic approach to extend plasma half-life. Allometric scaling ...

journal_title：Nature biotechnology

authors： Schellenberger V,Wang CW,Geething NC,Spink BJ,Campbell A,To W,Scholle MD,Yin Y,Yao Y,Bogin O,Cleland JL,Silverman J,Stemmer WP

更新日期：2009-12-01 00:00:00

abstract：:Foxtail millet (Setaria italica), a member of the Poaceae grass family, is an important food and fodder crop in arid regions and has potential for use as a C(4) biofuel. It is a model system for other biofuel grasses, including switchgrass and pearl millet. We produced a draft genome (∼423 Mb) anchored onto nine chrom...

journal_title：Nature biotechnology

authors： Zhang G,Liu X,Quan Z,Cheng S,Xu X,Pan S,Xie M,Zeng P,Yue Z,Wang W,Tao Y,Bian C,Han C,Xia Q,Peng X,Cao R,Yang X,Zhan D,Hu J,Zhang Y,Li H,Li H,Li N,Wang J,Wang C,Wang R,Guo T,Cai Y,Liu C,Xiang H,

更新日期：2012-05-13 00:00:00

abstract：:Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...

journal_title：Nature biotechnology

authors： Sarkizova S,Klaeger S,Le PM,Li LW,Oliveira G,Keshishian H,Hartigan CR,Zhang W,Braun DA,Ligon KL,Bachireddy P,Zervantonakis IK,Rosenbluth JM,Ouspenskaia T,Law T,Justesen S,Stevens J,Lane WJ,Eisenhaure T,Lan Zhang G,

更新日期：2020-02-01 00:00:00

abstract：:Genome editing with targeted nucleases and DNA donor templates homologous to the break site has proven challenging in human hematopoietic stem and progenitor cells (HSPCs), and particularly in the most primitive, long-term repopulating cell population. Here we report that combining electroporation of zinc finger nucle...

journal_title：Nature biotechnology

authors： Wang J,Exline CM,DeClercq JJ,Llewellyn GN,Hayward SB,Li PW,Shivak DA,Surosky RT,Gregory PD,Holmes MC,Cannon PM

更新日期：2015-12-01 00:00:00

abstract：:An approach to the systematic identification and quantification of the proteins contained in the microsomal fraction of cells is described. It consists of three steps: (1) preparation of microsomal fractions from cells or tissues representing different states; (2) covalent tagging of the proteins with isotope-coded af...

journal_title：Nature biotechnology

authors： Han DK,Eng J,Zhou H,Aebersold R

更新日期：2001-10-01 00:00:00

abstract：:In most tree-breeding programs worldwide, increasing the trees' growth rates and stem volumes and shortening their rotation times are important aims. Such trees would yield more biomass per unit area. Here we show that overexpressing a key regulatory gene in the biosynthesis of the plant hormone gibberellin (GA) in hy...

journal_title：Nature biotechnology

authors： Eriksson ME,Israelsson M,Olsson O,Moritz T

更新日期：2000-07-01 00:00:00

abstract：:Small-interfering RNAs (siRNAs) assemble into RISC, the RNA-induced silencing complex, which cleaves complementary mRNAs. Despite their fluctuating efficacy, siRNAs are widely used to assess gene function. Although this limitation could be ascribed, in part, to variations in the assembly and activation of RISC, downst...

journal_title：Nature biotechnology

authors： Tafer H,Ameres SL,Obernosterer G,Gebeshuber CA,Schroeder R,Martinez J,Hofacker IL

更新日期：2008-05-01 00:00:00

abstract：:Academic researchers have regularly ignored patents on key technologies as a strategy to maneuver around patent thickets and freedom-to-operate issues, but they may be more at risk than they realize. ...

journal_title：Nature biotechnology

authors： Yancey A,Stewart CN Jr

更新日期：2007-11-01 00:00:00

abstract：:The site-specific recombinases FLP and Cre are useful for genomic engineering in many living systems. Manipulation of their enzymatic properties offers a means to improve their applicability in different host organisms. We chose to manipulate the thermolability of FLP recombinase. A lacZ-based recombination assay in E...

journal_title：Nature biotechnology

authors： Buchholz F,Angrand PO,Stewart AF

更新日期：1998-07-01 00:00:00

abstract：:Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the latter. Here we investigate the effects of biological variation in HeLa cell lines using a systems-wide approach. We determine the degree of molecular and phenotypic variability across 14...

journal_title：Nature biotechnology

authors： Liu Y,Mi Y,Mueller T,Kreibich S,Williams EG,Van Drogen A,Borel C,Frank M,Germain PL,Bludau I,Mehnert M,Seifert M,Emmenlauer M,Sorg I,Bezrukov F,Bena FS,Zhou H,Dehio C,Testa G,Saez-Rodriguez J,Antonarakis SE,Hard

更新日期：2019-03-01 00:00:00

abstract：:The treatment of asthma and allergic rhinitis using unique, humanized anti-IgE monoclonal antibodies with very particular binding specificities is now supported by the results of multiple phase II and III human clinical studies. The therapeutic efficacy of this approach is attributable to several pharmacological mecha...

journal_title：Nature biotechnology

authors： Chang TW

更新日期：2000-02-01 00:00:00

abstract：:The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies and then labeled the immunocomplexes (one or zero labeled target pr...

journal_title：Nature biotechnology

authors： Rissin DM,Kan CW,Campbell TG,Howes SC,Fournier DR,Song L,Piech T,Patel PP,Chang L,Rivnak AJ,Ferrell EP,Randall JD,Provuncher GK,Walt DR,Duffy DC

更新日期：2010-06-01 00:00:00

abstract：:The specificity of biological regulatory mechanisms relies on selective interactions between different proteins in different cell types and in response to different extracellular signals. We describe a bimolecular fluorescence complementation (BiFC) approach for the simultaneous visualization of multiple protein inter...

journal_title：Nature biotechnology

authors： Hu CD,Kerppola TK

更新日期：2003-05-01 00:00:00

abstract：:Microbially catalyzed reactions, which occur in the natural sulfur cycle, have been integrated in a microbiological process to remove toxic metals from contaminated soils. Bioleaching using sulfuric acid produced by sulfur-oxidizing bacteria was followed by precipitation of the leachate metals as insoluble sulfides by...

journal_title：Nature biotechnology

authors： White C,Sharman AK,Gadd GM

更新日期：1998-06-01 00:00:00

abstract：:Fewer than half of all tandem mass spectrometry (MS/MS) spectra acquired in shotgun proteomics experiments are typically matched to a peptide with high confidence. Here we determine the identity of unassigned peptides using an ultra-tolerant Sequest database search that allows peptide matching even with modifications ...

journal_title：Nature biotechnology

authors： Chick JM,Kolippakkam D,Nusinow DP,Zhai B,Rad R,Huttlin EL,Gygi SP

更新日期：2015-07-01 00:00:00

abstract：:Influenza A virus leads to yearly epidemics and sporadic pandemics. Present prophylactic strategies focus on egg-grown, live, attenuated influenza vaccines (LAIVs), in which attenuation is generated by conferring temperature sensitivity onto the virus. Here we describe an alternative approach to attenuating influenza ...

journal_title：Nature biotechnology

authors： Perez JT,Pham AM,Lorini MH,Chua MA,Steel J,tenOever BR

更新日期：2009-06-01 00:00:00

abstract：:Detection of mutations and damaged DNA bases is important for the early diagnosis of genetic disease. Here we describe an electrocatalytic method for the detection of single-base mismatches as well as DNA base lesions in fully hybridized duplexes, based on charge transport through DNA films. Gold electrodes modified w...

journal_title：Nature biotechnology

authors： Boon EM,Ceres DM,Drummond TG,Hill MG,Barton JK

更新日期：2000-10-01 00:00:00

abstract：:Protein phosphorylation is a key post-translational modification regulating protein function in almost all cellular processes. Although tens of thousands of phosphorylation sites have been identified in human cells, approaches to determine the functional importance of each phosphosite are lacking. Here, we manually cu...

journal_title：Nature biotechnology

authors： Ochoa D,Jarnuczak AF,Viéitez C,Gehre M,Soucheray M,Mateus A,Kleefeldt AA,Hill A,Garcia-Alonso L,Stein F,Krogan NJ,Savitski MM,Swaney DL,Vizcaíno JA,Noh KM,Beltrao P

更新日期：2020-03-01 00:00:00

abstract：:Little is known about the consequences of releasing genetically engineered plants (GEP) into the environment. Using opine-producing GEP, we show that transgenic plants alter their biological environment, more precisely the root-associated bacterial populations. The alterations were both transgene-specific and target p...

journal_title：Nature biotechnology

authors： Oger P,Petit A,Dessaux Y

更新日期：1997-04-01 00:00:00

abstract：:RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic and clinical research, but the determinants of targeting specificity and the extent of off-target cleavage remain insufficiently understood. Using chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq), we mapped genom...

journal_title：Nature biotechnology

authors： Kuscu C,Arslan S,Singh R,Thorpe J,Adli M

更新日期：2014-07-01 00:00:00

abstract：:Despite recent accomplishments, significant challenges remain before cloning can become a routine part of transgenic animal production. ...

journal_title：Nature biotechnology

authors： Dove A

更新日期：2000-10-01 00:00:00

abstract：:Live cell imaging is a powerful method to study protein dynamics at the cell surface, but conventional imaging probes are bulky, or interfere with protein function, or dissociate from proteins after internalization. Here, we report technology for covalent, specific tagging of cellular proteins with chemical probes. Th...

journal_title：Nature biotechnology

authors： Fernández-Suárez M,Baruah H,Martínez-Hernández L,Xie KT,Baskin JM,Bertozzi CR,Ting AY

更新日期：2007-12-01 00:00:00