解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Engineered T cells are effective therapies against a range of malignancies, but current approaches rely on autologous T cells, which are difficult and expensive to manufacture. Efforts to develop potent allogeneic T cells that are not rejected by the recipient's immune system require abrogating both T- and natural kil...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0601-5
更新日期:2021-01-01 00:00:00
abstract::Current base editors (BEs) catalyze only base transitions (C to T and A to G) and cannot produce base transversions. Here we present BEs that cause C-to-A transversions in Escherichia coli and C-to-G transversions in mammalian cells. These glycosylase base editors (GBEs) consist of a Cas9 nickase, a cytidine deaminase...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0592-2
更新日期:2021-01-01 00:00:00
abstract::A detailed understanding of the function of neural networks and how they are supported by a dynamic vascular system requires fast three-dimensional imaging in thick tissues. Here we present confocal light field microscopy, a method that enables fast volumetric imaging in the brain at depths of hundreds of micrometers....
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0628-7
更新日期:2021-01-01 00:00:00
abstract::Comparing diverse single-cell RNA sequencing (scRNA-seq) datasets generated by different technologies and in different laboratories remains a major challenge. Here we address the need for guidance in choosing algorithms leading to accurate biological interpretations of varied data types acquired with different platfor...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-00748-9
更新日期:2020-12-21 00:00:00
abstract::Single-molecule long-read DNA sequencing with biological nanopores is fast and high-throughput but suffers reduced accuracy in homonucleotide stretches. We now combine the CsgG nanopore with the 35-residue N-terminal region of its extracellular interaction partner CsgF to produce a dual-constriction pore with improved...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0570-8
更新日期:2020-12-01 00:00:00
abstract::N6-Methyladenosine (m6A) is the most widespread internal messenger RNA modification in humans. Despite recent progress in understanding the biological roles of m6A, the inability to install m6A site specifically in individual transcripts has hampered efforts to elucidate causal relationships between the presence of a ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0572-6
更新日期:2020-12-01 00:00:00
abstract::Centromeric variation has been linked to cancer and infertility, but centromere sequences contain multiple tandem repeats and can only be assembled manually from long error-prone reads. Here we describe the centroFlye algorithm for centromere assembly using long error-prone reads, and apply it to assemble human centro...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0582-4
更新日期:2020-11-01 00:00:00
abstract::Correct reconstruction of macromolecular structure by cryo-electron microscopy (cryo-EM) relies on accurate determination of the orientation of single-particle images. For small (<100 kDa) DNA-binding proteins, obtaining particle images with sufficiently asymmetric features to correctly guide alignment is challenging....
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0716-8
更新日期:2020-10-19 00:00:00
abstract::Monitoring the T cell receptor (TCR) repertoire in health and disease can provide key insights into adaptive immune responses, but the accuracy of current TCR sequencing (TCRseq) methods is unclear. In this study, we systematically compared the results of nine commercial and academic TCRseq methods, including six rapi...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0656-3
更新日期:2020-09-07 00:00:00
abstract::De novo assembly of a human genome using nanopore long-read sequences has been reported, but it used more than 150,000 CPU hours and weeks of wall-clock time. To enable rapid human genome assembly, we present Shasta, a de novo long-read assembler, and polishing algorithms named MarginPolish and HELEN. Using a single P...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0503-6
更新日期:2020-09-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-020-0658-1
更新日期:2020-09-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-020-0578-0
更新日期:2020-08-01 00:00:00
abstract::The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively benchmarked. Here, we directly compare seven methods for single-cell and/or si...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0465-8
更新日期:2020-06-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41587-020-0510-7
更新日期:2020-05-01 00:00:00
abstract::The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing G bases. Here we report the evolution of three new SpCas9 variants that collectively recognize NRNH PAMs (where R is A or G and H is A, C or T) using ph...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0412-8
更新日期:2020-04-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41587-020-0427-1
更新日期:2020-03-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41587-020-0457-8
更新日期:2020-03-01 00:00:00
abstract::Single-cell RNA sequencing (scRNA-seq) enables the systematic identification of cell populations in a tissue, but characterizing their spatial organization remains challenging. We combine a microarray-based spatial transcriptomics method that reveals spatial patterns of gene expression using an array of spots, each ca...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0392-8
更新日期:2020-03-01 00:00:00
abstract::Protein phosphorylation is a key post-translational modification regulating protein function in almost all cellular processes. Although tens of thousands of phosphorylation sites have been identified in human cells, approaches to determine the functional importance of each phosphosite are lacking. Here, we manually cu...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0344-3
更新日期:2020-03-01 00:00:00
abstract::Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0322-9
更新日期:2020-02-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-019-0320-y
更新日期:2019-12-01 00:00:00
abstract::Multiple sequence alignments (MSAs) are used for structural1,2 and evolutionary predictions1,2, but the complexity of aligning large datasets requires the use of approximate solutions3, including the progressive algorithm4. Progressive MSA methods start by aligning the most similar sequences and subsequently incorpora...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0333-6
更新日期:2019-12-01 00:00:00
abstract::Expansions of short tandem repeats are genetic variants that have been implicated in several neuropsychiatric and other disorders, but their assessment remains challenging with current polymerase-based methods1-4. Here we introduce a CRISPR-Cas-based enrichment strategy for nanopore sequencing combined with an algorit...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0293-x
更新日期:2019-12-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-019-0292-y
更新日期:2019-11-01 00:00:00
abstract::Most microorganisms from all taxonomic levels are uncultured. Single-cell genomes and metagenomes continue to increase the known diversity of Bacteria and Archaea; however, while 'omics can be used to infer physiological or ecological roles for species in a community, most of these hypothetical roles remain unvalidate...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0260-6
更新日期:2019-11-01 00:00:00
abstract::The density and long-term stability of DNA make it an appealing storage medium, particularly for long-term data archiving. Existing DNA storage technologies involve the synthesis and sequencing of multiple nominally identical molecules in parallel, resulting in information redundancy. We report the development of enco...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0240-x
更新日期:2019-10-01 00:00:00
abstract::The clinical translation of cationic α-helical antimicrobial peptides (AMPs) has been hindered by structural instability, proteolytic degradation and in vivo toxicity from nonspecific membrane lysis. Although analyses of hydrophobic content and charge distribution have informed the design of synthetic AMPs with increa...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0222-z
更新日期:2019-10-01 00:00:00
abstract::Current tools for targeted RNA editing rely on the delivery of exogenous proteins or chemically modified guide RNAs, which may lead to aberrant effector activity, delivery barrier or immunogenicity. Here, we present an approach, called leveraging endogenous ADAR for programmable editing of RNA (LEAPER), that employs s...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0178-z
更新日期:2019-09-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-019-0253-5
更新日期:2019-09-01 00:00:00
abstract::The epicardium and its derivatives provide trophic and structural support for the developing and adult heart. Here we tested the ability of human embryonic stem cell (hESC)-derived epicardium to augment the structure and function of engineered heart tissue in vitro and to improve efficacy of hESC-cardiomyocyte grafts ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0197-9
更新日期:2019-08-01 00:00:00
abstract::The gold standard for clinical diagnosis of bacterial lower respiratory infections (LRIs) is culture, which has poor sensitivity and is too slow to guide early, targeted antimicrobial therapy. Metagenomic sequencing could identify LRI pathogens much faster than culture, but methods are needed to remove the large amoun...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0156-5
更新日期:2019-07-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-019-0167-2
更新日期:2019-07-01 00:00:00
abstract::Nitrogen-use efficiency of indica varieties of rice is superior to that of japonica varieties. We apply 16S ribosomal RNA gene profiling to characterize root microbiota of 68 indica and 27 japonica varieties grown in the field. We find that indica and japonica recruit distinct root microbiota. Notably, indica-enriched...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0104-4
更新日期:2019-06-01 00:00:00
abstract::Standardized benchmarking approaches are required to assess the accuracy of variants called from sequence data. Although variant-calling tools and the metrics used to assess their performance continue to improve, important challenges remain. Here, as part of the Global Alliance for Genomics and Health (GA4GH), we pres...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0054-x
更新日期:2019-05-01 00:00:00
abstract::Although mass spectrometry is well suited to identifying thousands of potential protein post-translational modifications (PTMs), it has historically been biased towards just a few. To measure the entire set of PTMs across diverse proteomes, software must overcome the dual challenges of covering enormous search spaces ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0067-5
更新日期:2019-04-01 00:00:00
abstract::In the version of this article initially published, Lena Dolman's second affiliation was given as Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK. The correct second affiliation is Ontario Institute for Cancer Research, Toronto, Ontario, Canada. The error has been corrected in the HTML ...
journal_title:Nature biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41587-019-0094-2
更新日期:2019-04-01 00:00:00
abstract::In the version of this article initially published, a GlaxoSmithKline compound referred to as GSK298559 was listed in Table 1 as a RIPK1 inhibitor in phase 1 trials for inflammatory bowel disease. The correct designation is GSK2983559; however, this compound is a RIPK2 inhibitor and thus should not have been included ...
journal_title:Nature biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41587-019-0082-6
更新日期:2019-04-01 00:00:00
abstract::Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the latter. Here we investigate the effects of biological variation in HeLa cell lines using a systems-wide approach. We determine the degree of molecular and phenotypic variability across 14...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0037-y
更新日期:2019-03-01 00:00:00
abstract::In the version of this article initially published, Betalutin was described as directing a radioisotope to CD45-expressing B cells; in fact, it directs it to CD37-expressing B cells. The error has been corrected in the HTML and PDF versions of the article. ...
journal_title:Nature biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41587-019-0030-5
更新日期:2019-02-01 00:00:00
abstract::Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq ena...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-018-0007-9
更新日期:2019-02-01 00:00:00