MALDI-TOF based mutation detection using tagged in vitro synthesized peptides.

abstract：:Reengineering the receptor footprints of adeno-associated virus (AAV) isolates may yield variants with improved properties for clinical applications. We generated a panel of synthetic AAV2 vectors by replacing a hexapeptide sequence in a previously identified heparan sulfate receptor footprint with corresponding resid...

journal_title：Nature biotechnology

authors： Asokan A,Conway JC,Phillips JL,Li C,Hegge J,Sinnott R,Yadav S,DiPrimio N,Nam HJ,Agbandje-McKenna M,McPhee S,Wolff J,Samulski RJ

更新日期：2010-01-01 00:00:00

abstract：:The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a give...

journal_title：Nature biotechnology

authors： Latouche JB,Sadelain M

更新日期：2000-04-01 00:00:00

abstract：:Pollen-mediated movement of transgenes from transplastomic oilseed rape (Brassica napus) into wild relatives will be avoided if chloroplasts are maternally transmitted. We assess the probability of chloroplast exchange between conventional oilseed rape and wild Brassica rapa to model the future behavior of transplasto...

journal_title：Nature biotechnology

authors： Scott SE,Wilkinson MJ

更新日期：1999-04-01 00:00:00

abstract：:The excitement and controversy surrounding the potential role of human embryonic stem (ES) cells in transplantation therapy have often overshadowed their potentially more important use as a basic research tool for understanding the development and function of human tissues. Human ES cells can proliferate without a kno...

journal_title：Nature biotechnology

authors： Xu RH,Chen X,Li DS,Li R,Addicks GC,Glennon C,Zwaka TP,Thomson JA

更新日期：2002-12-01 00:00:00

abstract：:Systemic lupus erythematosus (SLE) is a multisystem chronic inflammatory disease of unknown etiology that affects many organs, including the kidney. The presence of multiple autoantibodies and other immunological abnormalities point to basic defects in immunoregulatory controls that normally maintain self-tolerance. T...

journal_title：Nature biotechnology

authors： Marino M,Ruvo M,De Falco S,Fassina G

更新日期：2000-07-01 00:00:00

abstract：:Little is known about the consequences of releasing genetically engineered plants (GEP) into the environment. Using opine-producing GEP, we show that transgenic plants alter their biological environment, more precisely the root-associated bacterial populations. The alterations were both transgene-specific and target p...

journal_title：Nature biotechnology

authors： Oger P,Petit A,Dessaux Y

更新日期：1997-04-01 00:00:00

abstract：:Vaccination with dendritic cells (DCs) pulsed with antigenic peptides derived from various tumor antigens has great, but as yet significantly unrealized, potential in cancer treatment. Here, we describe a strategy for prolonged presentation of an MHC class I-restricted self-peptide on DCs through linkage of it to a ce...

journal_title：Nature biotechnology

authors： Wang RF,Wang HY

更新日期：2002-02-01 00:00:00

abstract：:Efficiently generating evidence of clinical utility is a major challenge for ensuring clinical adoption of valuable diagnostics. A new approach to reimbursement in the United States offers a balance between evidence and incentives for molecular diagnostic tests. ...

journal_title：Nature biotechnology

authors： Schulman KA,Tunis SR

更新日期：2010-11-01 00:00:00

abstract：:Individual genetic variation affects gene responsiveness to stimuli, often by influencing complex molecular circuits. Here we combine genomic and intermediate-scale transcriptional profiling with computational methods to identify variants that affect the responsiveness of genes to stimuli (responsiveness quantitative ...

journal_title：Nature biotechnology

authors： Gat-Viks I,Chevrier N,Wilentzik R,Eisenhaure T,Raychowdhury R,Steuerman Y,Shalek AK,Hacohen N,Amit I,Regev A

更新日期：2013-04-01 00:00:00

abstract：:Through sequential generations of random mutagenesis and screening, we have directed the evolution of an esterase for deprotection of an antibiotic p-nitrobenzyl ester in aqueous-organic solvents. Because rapid screening directly on the desired antibiotic (loracarbef) nucleus p-nitrobenzyl ester was not feasible, the ...

journal_title：Nature biotechnology

authors： Moore JC,Arnold FH

更新日期：1996-04-01 00:00:00

abstract：:Expansions of short tandem repeats are genetic variants that have been implicated in several neuropsychiatric and other disorders, but their assessment remains challenging with current polymerase-based methods1-4. Here we introduce a CRISPR-Cas-based enrichment strategy for nanopore sequencing combined with an algorit...

journal_title：Nature biotechnology

authors： Giesselmann P,Brändl B,Raimondeau E,Bowen R,Rohrandt C,Tandon R,Kretzmer H,Assum G,Galonska C,Siebert R,Ammerpohl O,Heron A,Schneider SA,Ladewig J,Koch P,Schuldt BM,Graham JE,Meissner A,Müller FJ

更新日期：2019-12-01 00:00:00

abstract：:Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the latter. Here we investigate the effects of biological variation in HeLa cell lines using a systems-wide approach. We determine the degree of molecular and phenotypic variability across 14...

journal_title：Nature biotechnology

authors： Liu Y,Mi Y,Mueller T,Kreibich S,Williams EG,Van Drogen A,Borel C,Frank M,Germain PL,Bludau I,Mehnert M,Seifert M,Emmenlauer M,Sorg I,Bezrukov F,Bena FS,Zhou H,Dehio C,Testa G,Saez-Rodriguez J,Antonarakis SE,Hard

更新日期：2019-03-01 00:00:00

abstract：:New cultivars with very erect leaves, which increase light capture for photosynthesis and nitrogen storage for grain filling, may have increased grain yields. Here we show that the erect leaf phenotype of a rice brassinosteroid-deficient mutant, osdwarf4-1, is associated with enhanced grain yields under conditions of ...

journal_title：Nature biotechnology

authors： Sakamoto T,Morinaka Y,Ohnishi T,Sunohara H,Fujioka S,Ueguchi-Tanaka M,Mizutani M,Sakata K,Takatsuto S,Yoshida S,Tanaka H,Kitano H,Matsuoka M

更新日期：2006-01-01 00:00:00

abstract：:Protein phosphorylation is a key post-translational modification regulating protein function in almost all cellular processes. Although tens of thousands of phosphorylation sites have been identified in human cells, approaches to determine the functional importance of each phosphosite are lacking. Here, we manually cu...

journal_title：Nature biotechnology

authors： Ochoa D,Jarnuczak AF,Viéitez C,Gehre M,Soucheray M,Mateus A,Kleefeldt AA,Hill A,Garcia-Alonso L,Stein F,Krogan NJ,Savitski MM,Swaney DL,Vizcaíno JA,Noh KM,Beltrao P

更新日期：2020-03-01 00:00:00

abstract：:A broad-specificity delta 9 desaturase gene was cloned from the cyanobacterium Anacystis nidulans. The enzyme introduces a cis-double bond at the delta 9 position of both 16 and 18 carbon saturated fatty acids linked to many kinds of membrane lipids. The gene was stably introduced into tobacco plants under transcripti...

journal_title：Nature biotechnology

authors： Ishizaki-Nishizawa O,Fujii T,Azuma M,Sekiguchi K,Murata N,Ohtani T,Toguri T

更新日期：1996-08-01 00:00:00

abstract：:Brief expression of pluripotency-associated factors such as Oct4, Klf4, Sox2 and c-Myc (OKSM), in combination with differentiation-inducing signals, has been reported to trigger transdifferentiation of fibroblasts into other cell types. Here we show that OKSM expression in mouse fibroblasts gives rise to both induced ...

journal_title：Nature biotechnology

authors： Bar-Nur O,Verheul C,Sommer AG,Brumbaugh J,Schwarz BA,Lipchina I,Huebner AJ,Mostoslavsky G,Hochedlinger K

更新日期：2015-07-01 00:00:00

abstract：:Thermostable enzymes combine catalytic specificity with the toughness required to withstand industrial reaction conditions. Stabilized enzymes also provide robust starting points for evolutionary improvement of other protein properties. We recently created a library of at least 2,300 new active chimeras of the biotech...

journal_title：Nature biotechnology

authors： Li Y,Drummond DA,Sawayama AM,Snow CD,Bloom JD,Arnold FH

更新日期：2007-09-01 00:00:00

abstract：:Can new approaches for deriving human stem cells circumnavigate existing patents that dominate embryonic stem cell intellectual property? ...

journal_title：Nature biotechnology

authors： Taymor KS,Scott CT,Greely HT

更新日期：2006-04-01 00:00:00

abstract：:Most microorganisms from all taxonomic levels are uncultured. Single-cell genomes and metagenomes continue to increase the known diversity of Bacteria and Archaea; however, while 'omics can be used to infer physiological or ecological roles for species in a community, most of these hypothetical roles remain unvalidate...

journal_title：Nature biotechnology

authors： Cross KL,Campbell JH,Balachandran M,Campbell AG,Cooper SJ,Griffen A,Heaton M,Joshi S,Klingeman D,Leys E,Yang Z,Parks JM,Podar M

更新日期：2019-11-01 00:00:00

abstract：:Glycoprotein hormones are noncovalent heterodimers comprised of a common alpha subunit and a hormone-specific beta subunit. Secretion and biologic action of these hormones are dependent on the formation of the heterodimer. The human LH beta subunit is unique among the other beta subunits in that it assembles inefficie...

journal_title：Nature biotechnology

authors： Garcia-Campayo V,Sato A,Hirsch B,Sugahara T,Muyan M,Hsueh AJ,Boime I

更新日期：1997-07-01 00:00:00

abstract：:Technologies that enable targeted manipulation of epigenetic marks could be used to precisely control cell phenotype or interrogate the relationship between the epigenome and transcriptional control. Here we describe a programmable, CRISPR-Cas9-based acetyltransferase consisting of the nuclease-null dCas9 protein fuse...

journal_title：Nature biotechnology

authors： Hilton IB,D'Ippolito AM,Vockley CM,Thakore PI,Crawford GE,Reddy TE,Gersbach CA

更新日期：2015-05-01 00:00:00

abstract：:DNA charge transport chemistry is found to provide a sensitive method for probing protein-dependent changes in DNA structure and enzymatic reactions. Here we describe the development of an electrochemical assay of protein binding to DNA-modified electrodes based upon the detection of associated perturbations in DNA ba...

journal_title：Nature biotechnology

authors： Boon EM,Salas JE,Barton JK

更新日期：2002-03-01 00:00:00

abstract：:The gold standard for clinical diagnosis of bacterial lower respiratory infections (LRIs) is culture, which has poor sensitivity and is too slow to guide early, targeted antimicrobial therapy. Metagenomic sequencing could identify LRI pathogens much faster than culture, but methods are needed to remove the large amoun...

journal_title：Nature biotechnology

authors： Charalampous T,Kay GL,Richardson H,Aydin A,Baldan R,Jeanes C,Rae D,Grundy S,Turner DJ,Wain J,Leggett RM,Livermore DM,O'Grady J

更新日期：2019-07-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Röst HL,Rosenberger G,Navarro P,Gillet L,Miladinović SM,Schubert OT,Wolski W,Collins BC,Malmström J,Malmström L,Aebersold R

更新日期：2020-03-01 00:00:00

abstract：:Detection of mutations and damaged DNA bases is important for the early diagnosis of genetic disease. Here we describe an electrocatalytic method for the detection of single-base mismatches as well as DNA base lesions in fully hybridized duplexes, based on charge transport through DNA films. Gold electrodes modified w...

journal_title：Nature biotechnology

authors： Boon EM,Ceres DM,Drummond TG,Hill MG,Barton JK

更新日期：2000-10-01 00:00:00

abstract：:Enzymatic removal of blood group ABO antigens to develop universal red blood cells (RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical pract...

journal_title：Nature biotechnology

authors： Liu QP,Sulzenbacher G,Yuan H,Bennett EP,Pietz G,Saunders K,Spence J,Nudelman E,Levery SB,White T,Neveu JM,Lane WS,Bourne Y,Olsson ML,Henrissat B,Clausen H

更新日期：2007-04-01 00:00:00

abstract：:Monitoring the T cell receptor (TCR) repertoire in health and disease can provide key insights into adaptive immune responses, but the accuracy of current TCR sequencing (TCRseq) methods is unclear. In this study, we systematically compared the results of nine commercial and academic TCRseq methods, including six rapi...

journal_title：Nature biotechnology

authors： Barennes P,Quiniou V,Shugay M,Egorov ES,Davydov AN,Chudakov DM,Uddin I,Ismail M,Oakes T,Chain B,Eugster A,Kashofer K,Rainer PP,Darko S,Ransier A,Douek DC,Klatzmann D,Mariotti-Ferrandiz E

更新日期：2020-09-07 00:00:00

abstract：:RNA-guided gene drives capable of spreading genomic alterations made in laboratory organisms through wild populations could be used to address environmental and public health problems. However, the possibility of unintended genome editing occurring through the escape of strains from laboratories, coupled with the pros...

journal_title：Nature biotechnology

authors： DiCarlo JE,Chavez A,Dietz SL,Esvelt KM,Church GM

更新日期：2015-12-01 00:00:00

abstract：:An analysis of US Federal Circuit decisions shows strategies for designing around patents. ...

journal_title：Nature biotechnology

authors： Wang SJ

更新日期：2008-05-01 00:00:00

abstract：:An approach to the systematic identification and quantification of the proteins contained in the microsomal fraction of cells is described. It consists of three steps: (1) preparation of microsomal fractions from cells or tissues representing different states; (2) covalent tagging of the proteins with isotope-coded af...

journal_title：Nature biotechnology

authors： Han DK,Eng J,Zhou H,Aebersold R

更新日期：2001-10-01 00:00:00