A policy approach to the development of molecular diagnostic tests.

abstract：:Systemic lupus erythematosus (SLE) is a multisystem chronic inflammatory disease of unknown etiology that affects many organs, including the kidney. The presence of multiple autoantibodies and other immunological abnormalities point to basic defects in immunoregulatory controls that normally maintain self-tolerance. T...

journal_title：Nature biotechnology

authors： Marino M,Ruvo M,De Falco S,Fassina G

更新日期：2000-07-01 00:00:00

abstract：:Evolution of eukaryotes is mediated by sexual recombination of parental genomes. Crossovers occur in random, but homologous, positions at a frequency that depends on DNA length. As exons occupy only 1% of the human genome and introns about 24%, by far most of the crossovers occur between exons, rather than inside. The...

journal_title：Nature biotechnology

authors： Kolkman JA,Stemmer WP

更新日期：2001-05-01 00:00:00

abstract：:We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...

journal_title：Nature biotechnology

authors： Xiao L,Feng Q,Liang S,Sonne SB,Xia Z,Qiu X,Li X,Long H,Zhang J,Zhang D,Liu C,Fang Z,Chou J,Glanville J,Hao Q,Kotowska D,Colding C,Licht TR,Wu D,Yu J,Sung JJ,Liang Q,Li J,Jia H,Lan Z,Tremaroli V,Dworzyn

更新日期：2015-10-01 00:00:00

abstract：:The construction of synthetic cell-cell communication networks can improve our quantitative understanding of naturally occurring signaling pathways and enhance our capabilities to engineer coordinated cellular behavior in cell populations. Towards accomplishing these goals in eukaryotes, we developed and analyzed two ...

journal_title：Nature biotechnology

authors： Chen MT,Weiss R

更新日期：2005-12-01 00:00:00

abstract：:Excessive and prolonged activity of inflammatory monocytes is a hallmark of many diseases with an inflammatory component. In such conditions, precise targeting of these cells could be therapeutically beneficial while sparing many essential functions of the innate immune system, thus limiting unwanted effects. Inflamma...

journal_title：Nature biotechnology

authors： Leuschner F,Dutta P,Gorbatov R,Novobrantseva TI,Donahoe JS,Courties G,Lee KM,Kim JI,Markmann JF,Marinelli B,Panizzi P,Lee WW,Iwamoto Y,Milstein S,Epstein-Barash H,Cantley W,Wong J,Cortez-Retamozo V,Newton A,Love K,

更新日期：2011-10-09 00:00:00

abstract：:After nearly 40 years of development, oligonucleotide therapeutics are nearing meaningful clinical productivity. One of the key advantages of oligonucleotide drugs is that their delivery and potency are derived primarily from the chemical structure of the oligonucleotide whereas their target is defined by the base seq...

journal_title：Nature biotechnology

authors： Khvorova A,Watts JK

更新日期：2017-03-01 00:00:00

abstract：:Isoflavones have drawn much attention because of their benefits to human health. These compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. Isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid...

journal_title：Nature biotechnology

authors： Jung W,Yu O,Lau SM,O'Keefe DP,Odell J,Fader G,McGonigle B

更新日期：2000-02-01 00:00:00

abstract：:Directed molecular evolution was applied to generate Cre recombinase variants that recognize a new DNA target sequence. Cre was adapted in a three-stage strategy to evolve recombinases to specifically recombine the new site. This complex multicycle task was made feasible by an improved directed-evolution procedure tha...

journal_title：Nature biotechnology

authors： Buchholz F,Stewart AF

更新日期：2001-11-01 00:00:00

abstract：:Tissue function depends on hierarchical structures extending from single cells ( approximately 10 microm) to functional subunits (100 microm-1 mm) that coordinate organ functions. Conventional cell culture disperses tissues into single cells while neglecting higher-order processes. The application of semiconductor-dri...

journal_title：Nature biotechnology

authors： Khetani SR,Bhatia SN

更新日期：2008-01-01 00:00:00

abstract：:Insertional mutagenesis represents a major hurdle to gene therapy and necessitates sensitive preclinical genotoxicity assays. Cdkn2a-/- mice are susceptible to a broad range of cancer-triggering genetic lesions. We exploited hematopoietic stem cells from these tumor-prone mice to assess the oncogenicity of prototypica...

journal_title：Nature biotechnology

authors： Montini E,Cesana D,Schmidt M,Sanvito F,Ponzoni M,Bartholomae C,Sergi Sergi L,Benedicenti F,Ambrosi A,Di Serio C,Doglioni C,von Kalle C,Naldini L

更新日期：2006-06-01 00:00:00

abstract：:The expression patterns of eukaryotic genomes are controlled by their chromatin structure, consisting of nucleosome subunits in which DNA of approximately 146 bp is wrapped around a core of 8 histone molecules. Post-translational histone modifications play an essential role in modifying chromatin structure. Here we ap...

journal_title：Nature biotechnology

authors： Roh TY,Ngau WC,Cui K,Landsman D,Zhao K

更新日期：2004-08-01 00:00:00

abstract：:Targeting of secreted and cell-surface proteins to the cell membrane is mediated by a short hydrophobic stretch of amino acids, termed the signal sequence. We have developed a method that detects signal sequences in cDNA fragments based on their ability to redirect a constitutively active mutant of a cytokine receptor...

journal_title：Nature biotechnology

authors： Kojima T,Kitamura T

更新日期：1999-05-01 00:00:00

abstract：:New cultivars with very erect leaves, which increase light capture for photosynthesis and nitrogen storage for grain filling, may have increased grain yields. Here we show that the erect leaf phenotype of a rice brassinosteroid-deficient mutant, osdwarf4-1, is associated with enhanced grain yields under conditions of ...

journal_title：Nature biotechnology

authors： Sakamoto T,Morinaka Y,Ohnishi T,Sunohara H,Fujioka S,Ueguchi-Tanaka M,Mizutani M,Sakata K,Takatsuto S,Yoshida S,Tanaka H,Kitano H,Matsuoka M

更新日期：2006-01-01 00:00:00

abstract：:A variety of strategies to incorporate unnatural amino acids into proteins have been pursued, but all have limitations with respect to technical accessibility, scalability, applicability to in vivo studies, or site specificity of amino acid incorporation. The ability to selectively introduce unnatural functional group...

journal_title：Nature biotechnology

authors： Santoro SW,Wang L,Herberich B,King DS,Schultz PG

更新日期：2002-10-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Röst HL,Rosenberger G,Navarro P,Gillet L,Miladinović SM,Schubert OT,Wolski W,Collins BC,Malmström J,Malmström L,Aebersold R

更新日期：2020-03-01 00:00:00

abstract：:A wealth of molecular interaction data is available in the literature, ranging from large-scale datasets to a single interaction confirmed by several different techniques. These data are all too often reported either as free text or in tables of variable format, and are often missing key pieces of information essentia...

journal_title：Nature biotechnology

authors： Orchard S,Salwinski L,Kerrien S,Montecchi-Palazzi L,Oesterheld M,Stümpflen V,Ceol A,Chatr-aryamontri A,Armstrong J,Woollard P,Salama JJ,Moore S,Wojcik J,Bader GD,Vidal M,Cusick ME,Gerstein M,Gavin AC,Superti-Furga G,

更新日期：2007-08-01 00:00:00

abstract：:A protocol was established for the introduction of DNA into embryogenic suspension-derived tissues of cassava via microparticle bombardment, for the selection of genetically transformed cells, and for the regeneration of fully transgenic plants from these cells. The plasmid DNA used for bombardment contained a gene en...

journal_title：Nature biotechnology

authors： Schöpke C,Taylor N,Cárcamo R,Konan NK,Marmey P,Henshaw GG,Beachy RN,Fauquet C

更新日期：1996-06-01 00:00:00

abstract：:Numerous biological processes involve the recognition of a specific pattern of binding sites on a target protein or surface. Although ligands displayed by disordered scaffolds form stochastic rather than specific patterns, theoretical models predict that recognition will occur between patterns that are characterized b...

journal_title：Nature biotechnology

authors： Rai P,Padala C,Poon V,Saraph A,Basha S,Kate S,Tao K,Mogridge J,Kane RS

更新日期：2006-05-01 00:00:00

abstract：:MicroRNAs (miRNAs) are increasingly implicated in the regulation of metastasis. Despite their potential as targets for anti-metastatic therapy, miRNAs have only been silenced in normal tissues of rodents and nonhuman primates. Therefore, the development of effective approaches for sequence-specific inhibition of miRNA...

journal_title：Nature biotechnology

authors： Ma L,Reinhardt F,Pan E,Soutschek J,Bhat B,Marcusson EG,Teruya-Feldstein J,Bell GW,Weinberg RA

更新日期：2010-04-01 00:00:00

abstract：:Thermostable enzymes combine catalytic specificity with the toughness required to withstand industrial reaction conditions. Stabilized enzymes also provide robust starting points for evolutionary improvement of other protein properties. We recently created a library of at least 2,300 new active chimeras of the biotech...

journal_title：Nature biotechnology

authors： Li Y,Drummond DA,Sawayama AM,Snow CD,Bloom JD,Arnold FH

更新日期：2007-09-01 00:00:00

abstract：:Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here we show that genome-wide in vivo chromatin interaction frequency data, which are measurable with chromosome conforma...

journal_title：Nature biotechnology

authors： Kaplan N,Dekker J

更新日期：2013-12-01 00:00:00

abstract：:Plant satellite RNAs generally reduce the level of helper virus accumulation and attenuate the disease symptoms induced by the helper virus that they depend upon for replication and packaging. As such, satellite RNAs could be used as biocontrol agents to reduce the level of disease in field crops, either by the applic...

journal_title：Nature biotechnology

authors： Palukaitis P,Roossinck MJ

更新日期：1996-10-01 00:00:00

abstract：:Pollen-mediated movement of transgenes from transplastomic oilseed rape (Brassica napus) into wild relatives will be avoided if chloroplasts are maternally transmitted. We assess the probability of chloroplast exchange between conventional oilseed rape and wild Brassica rapa to model the future behavior of transplasto...

journal_title：Nature biotechnology

authors： Scott SE,Wilkinson MJ

更新日期：1999-04-01 00:00:00

abstract：:A central hurdle in developing small interfering RNAs (siRNAs) as therapeutics is the inefficiency of their delivery across the plasma and endosomal membranes to the cytosol, where they interact with the RNA interference machinery. With the aim of improving endosomal release, a poorly understood and inefficient proces...

journal_title：Nature biotechnology

authors： Wittrup A,Ai A,Liu X,Hamar P,Trifonova R,Charisse K,Manoharan M,Kirchhausen T,Lieberman J

更新日期：2015-08-01 00:00:00

abstract：:Neuronal precursor cells are widespread in the forebrain ventricular/subventricular zone, and may provide a cellular substrate for brain repair. Clonal lines derived from single progenitors can become progressively less representative of their parental precursors with time and passage in vitro. We have developed an al...

journal_title：Nature biotechnology

authors： Wang S,Wu H,Jiang J,Delohery TM,Isdell F,Goldman SA

更新日期：1998-02-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Ienca M,Haselager P,Emanuel EJ

更新日期：2019-07-01 00:00:00

abstract：:The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...

journal_title：Nature biotechnology

authors： Giordano-Coltart J,Calkins CW

更新日期：2008-02-01 00:00:00

abstract：:The oncofetal fibronectin (B-FN) isoform is present in vessels of neoplastic tissues during angiogenesis but not in mature vessels. B-FN could therefore provide a target for diagnostic imaging and therapy of cancer. Phage display libraries have been used to isolate human antibody fragments with pan-species recognition...

journal_title：Nature biotechnology

authors： Neri D,Carnemolla B,Nissim A,Leprini A,Querzè G,Balza E,Pini A,Tarli L,Halin C,Neri P,Zardi L,Winter G

更新日期：1997-11-01 00:00:00

abstract：:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...

journal_title：Nature biotechnology

authors： Sarria S,Kruyer NS,Peralta-Yahya P

更新日期：2017-12-01 00:00:00

abstract：:Dietary omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) are usually derived from marine fish. Although production of both EPA and DHA has been engineered into land plants, including Arabidopsis, Camelina sativa and Brassica juncea, nei...

journal_title：Nature biotechnology

authors： Walsh TA,Bevan SA,Gachotte DJ,Larsen CM,Moskal WA,Merlo PA,Sidorenko LV,Hampton RE,Stoltz V,Pareddy D,Anthony GI,Bhaskar PB,Marri PR,Clark LM,Chen W,Adu-Peasah PS,Wensing ST,Zirkle R,Metz JG

更新日期：2016-08-01 00:00:00