Abstract：
:The oncofetal fibronectin (B-FN) isoform is present in vessels of neoplastic tissues during angiogenesis but not in mature vessels. B-FN could therefore provide a target for diagnostic imaging and therapy of cancer. Phage display libraries have been used to isolate human antibody fragments with pan-species recognition of this isoform. We describe the use of these fragments in nude mice to target an aggressive tumor (grafted F9 murine teratocarcinoma). Imaging in real time was done by infrared photodetection of a chemically coupled fluorophore. The targeting was improved by use of affinity-matured fragments with low kinetic dissociation rates (koff = 1.5 x 10(-4) s-1) and also by engineering dimeric fragments via a C-terminal amphipathic helix.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Neri D,Carnemolla B,Nissim A,Leprini A,Querzè G,Balza E,Pini A,Tarli L,Halin C,Neri P,Zardi L,Winter Gdoi
10.1038/nbt1197-1271subject
Has Abstractpub_date
1997-11-01 00:00:00pages
1271-5issue
12eissn
1087-0156issn
1546-1696journal_volume
15pub_type
杂志文章abstract：:Systemic gene delivery into muscle has been a major challenge for muscular dystrophy gene therapy, with capillary blood vessels posing the principle barrier and limiting vector dissemination. Previous efforts to deliver genes into multiple muscles have relied on isolated vessel perfusion or pharmacological interventio...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1073
更新日期：2005-03-01 00:00:00
abstract：:Whole-genome mRNA quantitation can be used to identify the genes that are most responsive to environmental or genotypic change. By searching for mutually similar DNA elements among the upstream non-coding DNA sequences of these genes, we can identify candidate regulatory motifs and corresponding candidate sets of core...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1098-939
更新日期：1998-10-01 00:00:00
abstract：:Transplantation of oligodendrocyte precursor cells (OPCs) is a promising potential therapeutic strategy for diseases affecting myelin. However, the derivation of engraftable OPCs from human pluripotent stem cells has proven difficult and primary OPCs are not readily available. Here we report the generation of induced ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2564
更新日期：2013-05-01 00:00:00
abstract：:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt.4022
更新日期：2017-12-01 00:00:00
abstract：:The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0208-183
更新日期：2008-02-01 00:00:00
abstract：:Epigenetic mechanisms integrate genetic and environmental causes of disease, but comprehensive genome-wide analyses of epigenetic modifications have not yet demonstrated robust association with common diseases. Using Illumina HumanMethylation450 arrays on 354 anti-citrullinated protein antibody-associated rheumatoid a...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2487
更新日期：2013-02-01 00:00:00
abstract：:Increasing the in vivo residence times of protein therapeutics could decrease their dosing frequencies. We show that genetic fusion of an unstructured recombinant polypeptide of 864 amino acids, called XTEN, to a peptide or protein provides an apparently generic approach to extend plasma half-life. Allometric scaling ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1588
更新日期：2009-12-01 00:00:00
abstract：:Methods used to sequence the transcriptome often produce more than 200 million short sequences. We introduce StringTie, a computational method that applies a network flow algorithm originally developed in optimization theory, together with optional de novo assembly, to assemble these complex data sets into transcripts...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3122
更新日期：2015-03-01 00:00:00
abstract：:Understanding how proteins and their complex interaction networks convert the genomic information into a dynamic living organism is a fundamental challenge in biological sciences. As an important step towards understanding the systems biology of a complex eukaryote, we cataloged 63% of the predicted Drosophila melanog...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1300
更新日期：2007-05-01 00:00:00
abstract：:Reengineering the receptor footprints of adeno-associated virus (AAV) isolates may yield variants with improved properties for clinical applications. We generated a panel of synthetic AAV2 vectors by replacing a hexapeptide sequence in a previously identified heparan sulfate receptor footprint with corresponding resid...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1599
更新日期：2010-01-01 00:00:00
abstract：:We have identified a heptapeptide with high affinity to rheumatoid arthritis-associated class II major histocompatibility (MHC) molecules. Using a model of its interaction with the class II binding site, a variety of mimetic substitutions were introduced into the peptide. Several unnatural amino acids and dipeptide mi...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/9865
更新日期：1999-06-01 00:00:00
abstract：:Neuronal precursor cells are widespread in the forebrain ventricular/subventricular zone, and may provide a cellular substrate for brain repair. Clonal lines derived from single progenitors can become progressively less representative of their parental precursors with time and passage in vitro. We have developed an al...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0298-196
更新日期：1998-02-01 00:00:00
abstract：:Lysosomal storage diseases are treated with human lysosomal enzymes produced in mammalian cells. Such enzyme therapeutics contain relatively low levels of mannose-6-phosphate, which is required to target them to the lysosomes of patient cells. Here we describe a method for increasing mannose-6-phosphate modification o...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2427
更新日期：2012-12-01 00:00:00
abstract：:The sap-sucking insects (order Hemiptera), including aphids, planthoppers, whiteflies and stink bugs, present one of the greatest challenges for pest management in global agriculture. Insect neurotoxins offer an alternative to chemical insecticides for controlling these pests, but require delivery into the insect hemo...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2753
更新日期：2014-01-01 00:00:00
abstract：:We describe protein interaction quantitation (PIQ), a computational method for modeling the magnitude and shape of genome-wide DNase I hypersensitivity profiles to identify transcription factor (TF) binding sites. Through the use of machine-learning techniques, PIQ identified binding sites for >700 TFs from one DNase ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2798
更新日期：2014-02-01 00:00:00
abstract：:We describe wavelength-shifting molecular beacons, which are nucleic acid hybridization probes that fluoresce in a variety of different colors, yet are excited by a common monochromatic light source. The twin functions of absorption of energy from the excitation light and emission of that energy in the form of fluores...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/81192
更新日期：2000-11-01 00:00:00
abstract：:The construction of synthetic cell-cell communication networks can improve our quantitative understanding of naturally occurring signaling pathways and enhance our capabilities to engineer coordinated cellular behavior in cell populations. Towards accomplishing these goals in eukaryotes, we developed and analyzed two ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1162
更新日期：2005-12-01 00:00:00
abstract：:Ensuring adequate manufacturing capacity for recombinant and monoclonal antibodies is one of the greatest challenges facing the biotech industry. The following factors should be taken into account when designing product development and production strategies. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0905-1054
更新日期：2005-09-01 00:00:00
abstract：:Despite recent advances in optogenetics, it remains challenging to manipulate gene expression in specific populations of neurons. We present a dual-protein switch system, Cal-Light, that translates neuronal-activity-mediated calcium signaling into gene expression in a light-dependent manner. In cultured neurons and br...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3902
更新日期：2017-09-01 00:00:00
abstract：:Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments? ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1206-1504
更新日期：2006-12-01 00:00:00
abstract：:Glycoprotein hormones are noncovalent heterodimers comprised of a common alpha subunit and a hormone-specific beta subunit. Secretion and biologic action of these hormones are dependent on the formation of the heterodimer. The human LH beta subunit is unique among the other beta subunits in that it assembles inefficie...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0797-663
更新日期：1997-07-01 00:00:00
abstract：:We describe a novel approach to design a set of primers selective for large groups of genes. This method is based on the distribution frequency of all nucleotide combinations (octa- to decanucleotides), and the combined ability of primer pairs, based on these oligonucleotides, to detect genes. By analyzing 1000 human ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0796-857
更新日期：1996-07-01 00:00:00
abstract：:Although mass spectrometry has become a powerful tool for the functional analysis of biological systems, complete proteome characterization cannot yet be achieved. Instead, the sheer complexity of living organisms demands fractionation of cellular extracts to enable more targeted analyses. Here, we introduce the conce...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1076
更新日期：2005-04-01 00:00:00
abstract：:Through sequential generations of random mutagenesis and screening, we have directed the evolution of an esterase for deprotection of an antibiotic p-nitrobenzyl ester in aqueous-organic solvents. Because rapid screening directly on the desired antibiotic (loracarbef) nucleus p-nitrobenzyl ester was not feasible, the ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0496-458
更新日期：1996-04-01 00:00:00
abstract：:An analysis of US Federal Circuit decisions shows strategies for designing around patents. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0508-519
更新日期：2008-05-01 00:00:00
abstract：:Despite developments in targeted gene sequencing and whole-genome analysis techniques, the robust detection of all genetic variation, including structural variants, in and around genes of interest and in an allele-specific manner remains a challenge. Here we present targeted locus amplification (TLA), a strategy to se...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2959
更新日期：2014-10-01 00:00:00
abstract：:High-throughput genetic screens have become essential tools for studying a wide variety of biological processes. Here we experimentally compare systems based on clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) or its transcriptionally repressive variant, CRISPR-inter...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3536
更新日期：2016-06-01 00:00:00
abstract：:We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3353
更新日期：2015-10-01 00:00:00
abstract：:The site-specific recombinases FLP and Cre are useful for genomic engineering in many living systems. Manipulation of their enzymatic properties offers a means to improve their applicability in different host organisms. We chose to manipulate the thermolability of FLP recombinase. A lacZ-based recombination assay in E...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0798-657
更新日期：1998-07-01 00:00:00
abstract：:DNA microarrays have the ability to analyze the expression of thousands of the same set of genes under at least two different experimental conditions. However, DNA microarrays require substantial amounts of RNA to generate the probes, especially when bacterial RNA is used for hybridization (50 microg of bacterial tota...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/76543
更新日期：2000-06-01 00:00:00
