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Isolation of neuronal precursors by sorting embryonic forebrain transfected with GFP regulated by the T alpha 1 tubulin promoter.

Abstract：

:Neuronal precursor cells are widespread in the forebrain ventricular/subventricular zone, and may provide a cellular substrate for brain repair. Clonal lines derived from single progenitors can become progressively less representative of their parental precursors with time and passage in vitro. We have developed an alternative strategy for the isolation and enrichment of precursor cells, by fluorescence-activated cell sorting of forebrain cells transfected with the gene for green fluorescent protein, driven by the neuronal T alpha 1 tubulin promoter. Using this approach, neural precursors and young neurons can be identified and selectively harvested from a variety of samples, including both avian and mammalian forebrains at different developmental stages.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Wang S,Wu H,Jiang J,Delohery TM,Isdell F,Goldman SA

doi

10.1038/nbt0298-196

subject

Has Abstract

pub_date

1998-02-01 00:00:00

pages

196-201

issue

2

eissn

1087-0156

issn

1546-1696

journal_volume

16

pub_type

杂志文章

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