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Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content.

Abstract：

:Tocotrienols are the primary form of vitamin E in seeds of most monocot plants, including cereals such as rice and wheat. As potent antioxidants, tocotrienols contribute to the nutritive value of cereal grains in human and livestock diets. cDNAs encoding homogentisic acid geranylgeranyl transferase (HGGT), which catalyzes the committed step of tocotrienol biosynthesis, were isolated from barley, wheat and rice seeds. Transgenic expression of the barley HGGT in Arabidopsis thaliana leaves resulted in accumulation of tocotrienols, which were absent from leaves of nontransformed plants, and a 10- to 15-fold increase in total vitamin E antioxidants (tocotrienols plus tocopherols). Overexpression of the barley HGGT in corn seeds resulted in an increase in tocotrienol and tocopherol content of as much as six-fold. These results provide insight into the genetic basis for tocotrienol biosynthesis in plants and demonstrate the ability to enhance the antioxidant content of crops by introduction of an enzyme that redirects metabolic flux.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Cahoon EB,Hall SE,Ripp KG,Ganzke TS,Hitz WD,Coughlan SJ

doi

10.1038/nbt853

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

1082-7

issue

9

eissn

1087-0156

issn

1546-1696

pii

nbt853

journal_volume

21

pub_type

杂志文章

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