Abstract：
:Mice repopulated with human hematopoietic cells are a powerful tool for the study of human hematopoiesis and immune function in vivo. However, existing humanized mouse models cannot support development of human innate immune cells, including myeloid cells and natural killer (NK) cells. Here we describe two mouse strains called MITRG and MISTRG, in which human versions of four genes encoding cytokines important for innate immune cell development are knocked into their respective mouse loci. The human cytokines support the development and function of monocytes, macrophages and NK cells derived from human fetal liver or adult CD34(+) progenitor cells injected into the mice. Human macrophages infiltrated a human tumor xenograft in MITRG and MISTRG mice in a manner resembling that observed in tumors obtained from human patients. This humanized mouse model may be used to model the human immune system in scenarios of health and pathology, and may enable evaluation of therapeutic candidates in an in vivo setting relevant to human physiology.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Rongvaux A,Willinger T,Martinek J,Strowig T,Gearty SV,Teichmann LL,Saito Y,Marches F,Halene S,Palucka AK,Manz MG,Flavell RAdoi
10.1038/nbt.2858subject
Has Abstractpub_date
2014-04-01 00:00:00pages
364-72issue
4eissn
1087-0156issn
1546-1696pii
nbt.2858journal_volume
32pub_type
杂志文章abstract：:Thermostable enzymes combine catalytic specificity with the toughness required to withstand industrial reaction conditions. Stabilized enzymes also provide robust starting points for evolutionary improvement of other protein properties. We recently created a library of at least 2,300 new active chimeras of the biotech...
journal_title：Nature biotechnology
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doi：10.1038/nbt1333
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abstract：:The efficiency of both phage display in Escherichia coli and periplasmic expression of recombinant proteins may be limited by the same periplasmic folding steps. To search for E. coli factors that improve the efficiency of both procedures, a library of E. coli proteins was coexpressed in a phagemid vector that contain...
journal_title：Nature biotechnology
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abstract：:Protein phosphorylation is a key post-translational modification regulating protein function in almost all cellular processes. Although tens of thousands of phosphorylation sites have been identified in human cells, approaches to determine the functional importance of each phosphosite are lacking. Here, we manually cu...
journal_title：Nature biotechnology
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt816
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journal_title：Nature biotechnology
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pub_type： 杂志文章
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abstract：:Efficiently generating evidence of clinical utility is a major challenge for ensuring clinical adoption of valuable diagnostics. A new approach to reimbursement in the United States offers a balance between evidence and incentives for molecular diagnostic tests. ...
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journal_title：Nature biotechnology
pub_type： 杂志文章
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更新日期：2016-02-01 00:00:00
abstract：:Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light. Here we describe controlled light-exposure microscopy (CLEM), a simple imagi...
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pub_type： 杂志文章
doi：10.1038/nbt1278
更新日期：2007-02-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt986
更新日期：2004-07-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/10883
更新日期：1999-07-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0402-353
更新日期：2002-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1038/nbt0104-109
更新日期：2004-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2005-12-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/80301
更新日期：2000-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1038/s41587-020-0582-4
更新日期：2020-11-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt965
更新日期：2004-05-01 00:00:00
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更新日期：2017-07-01 00:00:00
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doi：10.1038/nbt1335
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更新日期：2010-01-01 00:00:00
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更新日期：1999-12-01 00:00:00
abstract：:Circular RNA transcripts were first identified in the early 1990s but knowledge of these species has remained limited, as their study through traditional methods of RNA analysis has been difficult. Now, novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have allowed compr...
journal_title：Nature biotechnology
pub_type： 杂志文章
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更新日期：2014-05-01 00:00:00
abstract：:Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in cult...
journal_title：Nature biotechnology
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更新日期：1999-02-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章,评审
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更新日期：2017-03-01 00:00:00
abstract：:The interaction between the HSP90 chaperone and its client kinases is sensitive to the conformational status of the kinase, and stabilization of the kinase fold by small molecules strongly decreases chaperone interaction. Here we exploit this observation and assay small-molecule binding to kinases in living cells, usi...
journal_title：Nature biotechnology
pub_type： 杂志文章
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更新日期：2013-07-01 00:00:00
abstract：:CD4 functions as a major T-cell surface receptor for human immunodeficiency virus by binding the human immunodeficiency virus type 1 (HIV-1) envelope protein gp120 with relatively high affinity. We have developed constrained aromatically modified analogs of the secondary structures of the first domain of CD4 in order ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0297-150
更新日期：1997-02-01 00:00:00
