Abstract:
:By using an engineered, self-assembling, proteinaceous, 2-nm pore equipped with a metal-actuated switch, a technique to reversibly permeabilize the plasma membrane to small molecules (approximately 1000 Da) has been developed. We have demonstrated the dose-dependent permeabilization of fibroblasts by pores designed to be blocked and unblocked by the addition and removal of microM concentrations of Zn2+. Further, we have shown that the activity of the switch allows permeabilized cells to maintain viability and ultrastructural integrity following the unconstrained flux of small molecules. This ability to control the transmembrane influx and efflux of molecules and thereby vary the intracellular environment yet maintain cell viability will impact an array of biological and medical problems.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Russo MJ,Bayley H,Toner Mdoi
10.1038/nbt0397-278subject
Has Abstractpub_date
1997-03-01 00:00:00pages
278-82issue
3eissn
1087-0156issn
1546-1696journal_volume
15pub_type
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