Abstract:
:Debates on patenting DNA must evolve to reflect the global decline in filings and regional disparities in patenting activity.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Hopkins MM,Mahdi S,Patel P,Thomas SMdoi
10.1038/nbt0207-185subject
Has Abstractpub_date
2007-02-01 00:00:00pages
185-7issue
2eissn
1087-0156issn
1546-1696pii
nbt0207-185journal_volume
25pub_type
杂志文章,评审abstract::The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and dow...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1132
更新日期:2005-09-01 00:00:00
abstract::Targeting of secreted and cell-surface proteins to the cell membrane is mediated by a short hydrophobic stretch of amino acids, termed the signal sequence. We have developed a method that detects signal sequences in cDNA fragments based on their ability to redirect a constitutively active mutant of a cytokine receptor...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/8666
更新日期:1999-05-01 00:00:00
abstract::We have developed a strategy for improving the stimulation of T cells during immune responses by constructing recombinant antibodies that enhance the delivery of antigen to antigen-presenting cells, such as B cells. These antibodies have variable regions specific for surface molecules on B cells, and a constant region...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/10883
更新日期:1999-07-01 00:00:00
abstract::Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light. Here we describe controlled light-exposure microscopy (CLEM), a simple imagi...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1278
更新日期:2007-02-01 00:00:00
abstract::We describe protein interaction quantitation (PIQ), a computational method for modeling the magnitude and shape of genome-wide DNase I hypersensitivity profiles to identify transcription factor (TF) binding sites. Through the use of machine-learning techniques, PIQ identified binding sites for >700 TFs from one DNase ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2798
更新日期:2014-02-01 00:00:00
abstract::Antifreeze proteins (AFPs) are found in cold-adapted organisms and have the unusual ability to bind to and inhibit the growth of ice crystals. However, the underlying molecular basis of their ice-binding activity is unclear because of the difficulty of studying the AFP-ice interaction directly and the lack of a common...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1224
更新日期:2006-07-01 00:00:00
abstract::Effective proteome-wide strategies that distinguish the N-termini of proteins from the N-termini of their protease cleavage products would accelerate identification of the substrates of proteases with broad or unknown specificity. Our approach, named terminal amine isotopic labeling of substrates (TAILS), addresses th...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1611
更新日期:2010-03-01 00:00:00
abstract::Despite recent advances in optogenetics, it remains challenging to manipulate gene expression in specific populations of neurons. We present a dual-protein switch system, Cal-Light, that translates neuronal-activity-mediated calcium signaling into gene expression in a light-dependent manner. In cultured neurons and br...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3902
更新日期:2017-09-01 00:00:00
abstract::We show that transplantation of adult bone marrow-derived cells expressing c-kit reduces hyperglycemia in mice with streptozotocin-induced pancreatic damage. Although quantitative analysis of the pancreas revealed a low frequency of donor insulin-positive cells, these cells were not present at the onset of blood gluco...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt841
更新日期:2003-07-01 00:00:00
abstract::A protocol was established for the introduction of DNA into embryogenic suspension-derived tissues of cassava via microparticle bombardment, for the selection of genetically transformed cells, and for the regeneration of fully transgenic plants from these cells. The plasmid DNA used for bombardment contained a gene en...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0696-731
更新日期:1996-06-01 00:00:00
abstract::We present 1,003 reference genomes that were sequenced as part of the Genomic Encyclopedia of Bacteria and Archaea (GEBA) initiative, selected to maximize sequence coverage of phylogenetic space. These genomes double the number of existing type strains and expand their overall phylogenetic diversity by 25%. Comparativ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3886
更新日期:2017-07-01 00:00:00
abstract::Evolution of eukaryotes is mediated by sexual recombination of parental genomes. Crossovers occur in random, but homologous, positions at a frequency that depends on DNA length. As exons occupy only 1% of the human genome and introns about 24%, by far most of the crossovers occur between exons, rather than inside. The...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/88084
更新日期:2001-05-01 00:00:00
abstract::The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a give...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/74455
更新日期:2000-04-01 00:00:00
abstract::Mycobacterium tuberculosis is a natural mutant in oxyR, a close homolog of the central regulator of peroxide stress response in enteric bacteria. Inactivation of oxyR is specific for M. tuberculosis and other members of the M. tuberculosis complex. This phenomenon appears as a paradox due to the ability of this organi...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1196-1557
更新日期:1996-11-01 00:00:00
abstract::Despite developments in targeted gene sequencing and whole-genome analysis techniques, the robust detection of all genetic variation, including structural variants, in and around genes of interest and in an allele-specific manner remains a challenge. Here we present targeted locus amplification (TLA), a strategy to se...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2959
更新日期:2014-10-01 00:00:00
abstract::Multiple intravenous injections of a cDNA library, derived from human melanoma cell lines and expressed using the highly immunogenic vector vesicular stomatitis virus (VSV), cured mice with established melanoma tumors. Successful tumor eradication was associated with the ability of mouse lymphoid cells to mount a tumo...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2157
更新日期:2012-03-18 00:00:00
abstract::Directed molecular evolution was applied to generate Cre recombinase variants that recognize a new DNA target sequence. Cre was adapted in a three-stage strategy to evolve recombinases to specifically recombine the new site. This complex multicycle task was made feasible by an improved directed-evolution procedure tha...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1101-1047
更新日期:2001-11-01 00:00:00
abstract::To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently form...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0598-431
更新日期:1998-05-01 00:00:00
abstract::New cultivars with very erect leaves, which increase light capture for photosynthesis and nitrogen storage for grain filling, may have increased grain yields. Here we show that the erect leaf phenotype of a rice brassinosteroid-deficient mutant, osdwarf4-1, is associated with enhanced grain yields under conditions of ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1173
更新日期:2006-01-01 00:00:00
abstract::Most microbes in the biosphere remain unculturable. Whole genome shotgun (WGS) sequencing of environmental DNA (metagenomics) can be used to study the genetic and metabolic properties of natural microbial communities. However, in communities of high complexity, metagenomics fails to link specific microbes to specific ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1488
更新日期:2008-09-01 00:00:00
abstract::Previous reports have demonstrated the growth of undifferentiated human embryonic stem (HES) cells on mouse embryonic fibroblast (MEF) feeders and on laminin- or Matrigel-coated plastic surfaces supplemented with MEF-conditioned medium. These xenosupport systems run the risk of cross-transfer of animal pathogens from ...
journal_title:Nature biotechnology
pub_type:
doi:10.1038/nbt726
更新日期:2002-09-01 00:00:00
abstract::High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequen...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt836
更新日期:2003-07-01 00:00:00
abstract::We describe tethered conformation capture (TCC), a method for genome-wide mapping of chromatin interactions. By performing ligations on solid substrates rather than in solution, TCC substantially enhances the signal-to-noise ratio, thereby facilitating a detailed analysis of interactions within and between chromosomes...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2057
更新日期:2011-12-25 00:00:00
abstract::RNA interference (RNAi) is a conserved sequence-specific, gene-silencing mechanism that is induced by double-stranded RNA. RNAi holds great promise as a novel nucleic acid-based therapeutic against a wide variety of diseases, including cancer, infectious diseases and genetic disorders. Antiviral RNAi strategies have r...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt1369
更新日期:2007-12-01 00:00:00
abstract::Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel profiling of transcriptomes. However, assessing differential expression across multiple individuals has been hampered by inefficient sample processing and technical batch effects. Here we describe a computational tool, demuxlet, that...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4042
更新日期:2018-01-01 00:00:00
abstract::Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the latter. Here we investigate the effects of biological variation in HeLa cell lines using a systems-wide approach. We determine the degree of molecular and phenotypic variability across 14...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0037-y
更新日期:2019-03-01 00:00:00
abstract::Noncoding RNAs (ncRNAs) are emerging as key molecules in human cancer, with the potential to serve as novel markers of disease and to reveal uncharacterized aspects of tumor biology. Here we discover 121 unannotated prostate cancer-associated ncRNA transcripts (PCATs) by ab initio assembly of high-throughput sequencin...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1914
更新日期:2011-07-31 00:00:00
abstract::Comparing diverse single-cell RNA sequencing (scRNA-seq) datasets generated by different technologies and in different laboratories remains a major challenge. Here we address the need for guidance in choosing algorithms leading to accurate biological interpretations of varied data types acquired with different platfor...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-00748-9
更新日期:2020-12-21 00:00:00
abstract::Efficient analysis of very large amounts of raw data for peptide identification and protein quantification is a principal challenge in mass spectrometry (MS)-based proteomics. Here we describe MaxQuant, an integrated suite of algorithms specifically developed for high-resolution, quantitative MS data. Using correlatio...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1511
更新日期:2008-12-01 00:00:00
abstract::The study of induced pluripotency often relies on experimental approaches that average measurements across a large population of cells, the majority of which do not become pluripotent. Here we used high-resolution, time-lapse imaging to trace the reprogramming process over 2 weeks from single mouse embryonic fibroblas...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1632
更新日期:2010-05-01 00:00:00