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Using virally expressed melanoma cDNA libraries to identify tumor-associated antigens that cure melanoma.

Abstract：

:Multiple intravenous injections of a cDNA library, derived from human melanoma cell lines and expressed using the highly immunogenic vector vesicular stomatitis virus (VSV), cured mice with established melanoma tumors. Successful tumor eradication was associated with the ability of mouse lymphoid cells to mount a tumor-specific CD4(+) interleukin (IL)-17 recall response in vitro. We used this characteristic IL-17 response to screen the VSV-cDNA library and identified three different VSV-cDNA virus clones that, when used in combination but not alone, achieved the same efficacy against tumors as the complete parental virus library. VSV-expressed cDNA libraries can therefore be used to identify tumor rejection antigens that can cooperate to induce anti-tumor responses. This technology should be applicable to antigen discovery for other cancers, as well as for other diseases in which immune reactivity against more than one target antigen contributes to disease pathology.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Pulido J,Kottke T,Thompson J,Galivo F,Wongthida P,Diaz RM,Rommelfanger D,Ilett E,Pease L,Pandha H,Harrington K,Selby P,Melcher A,Vile R

doi

10.1038/nbt.2157

subject

Has Abstract

pub_date

2012-03-18 00:00:00

pages

337-43

issue

4

eissn

1087-0156

issn

1546-1696

pii

nbt.2157

journal_volume

30

pub_type

杂志文章

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