Abstract:
:Transgenic crops are approved for release in some countries, while many more countries are wrestling with the issue of how to conduct risk assessments. Controls on field trials often include monitoring of horizontal gene transfer (HGT) from crops to surrounding soil microorganisms. Our analysis of antibiotic-resistant bacteria and of the sensitivity of current techniques for monitoring HGT from transgenic plants to soil microorganisms has two major implications for field trial assessments of transgenic crops: first, HGT from transgenic plants to microbes could still have an environmental impact at a frequency approximately a trillion times lower than the current risk assessment literature estimates the frequency to be; and second, current methods of environmental sampling to capture genes or traits in a recombinant are too insensitive for monitoring evolution by HGT. A model for HGT involving iterative short-patch events explains how HGT can occur at high frequencies but be detected at extremely low frequencies.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Heinemann JA,Traavik Tdoi
10.1038/nbt1009subject
Has Abstractpub_date
2004-09-01 00:00:00pages
1105-9issue
9eissn
1087-0156issn
1546-1696pii
nbt1009journal_volume
22pub_type
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