Problems in monitoring horizontal gene transfer in field trials of transgenic plants.

Abstract:

:Transgenic crops are approved for release in some countries, while many more countries are wrestling with the issue of how to conduct risk assessments. Controls on field trials often include monitoring of horizontal gene transfer (HGT) from crops to surrounding soil microorganisms. Our analysis of antibiotic-resistant bacteria and of the sensitivity of current techniques for monitoring HGT from transgenic plants to soil microorganisms has two major implications for field trial assessments of transgenic crops: first, HGT from transgenic plants to microbes could still have an environmental impact at a frequency approximately a trillion times lower than the current risk assessment literature estimates the frequency to be; and second, current methods of environmental sampling to capture genes or traits in a recombinant are too insensitive for monitoring evolution by HGT. A model for HGT involving iterative short-patch events explains how HGT can occur at high frequencies but be detected at extremely low frequencies.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Heinemann JA,Traavik T

doi

10.1038/nbt1009

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

1105-9

issue

9

eissn

1087-0156

issn

1546-1696

pii

nbt1009

journal_volume

22

pub_type

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