Abstract：
:A bacteriophage lambda surface expression system, lambda foo, was used for epitope mapping of human galectin-3. We constructed random epitope and peptide libraries and compared their efficiencies in the mapping. The galectin-3 cDNA was randomly digested by DNase 1 to make random epitope libraries. The libraries were screened by affinity selection using a microtiter plate coated with monoclonal antibodies. Direct DNA sequencing of the selected clones defined two distinct epitope sites consisting of nine and 11 amino-acid residues. Affinity selection of random peptide libraries recovered a number of sequences that were similar to each other but distinct from the galectin-3 sequence. These results demonstrate that a single affinity selection of epitope libraries with antibodies is able to define an epitope determinant as small as nine residues long and is more efficient in epitope mapping than random peptide libraries.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Kuwabara I,Maruyama H,Mikawa YG,Zuberi RI,Liu FT,Maruyama INdoi
10.1038/nbt0197-74subject
Has Abstractpub_date
1997-01-01 00:00:00pages
74-8issue
1eissn
1087-0156issn
1546-1696journal_volume
15pub_type
杂志文章abstract：:RNA-interference (RNAi) is a potent mechanism, conserved from plants to humans for specific silencing of genes, which holds promise for functional genomics and gene-targeted therapies. Here we show that bacteria engineered to produce a short hairpin RNA (shRNA) targeting a mammalian gene induce trans-kingdom RNAi in v...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1211
更新日期：2006-06-01 00:00:00
abstract：:St. Louis wants to become a hub of agricultural biotechnology. All it needs is more start-ups and funds. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0408-471
更新日期：2008-04-01 00:00:00
abstract：:Multiple sequence alignments (MSAs) are used for structural1,2 and evolutionary predictions1,2, but the complexity of aligning large datasets requires the use of approximate solutions3, including the progressive algorithm4. Progressive MSA methods start by aligning the most similar sequences and subsequently incorpora...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0333-6
更新日期：2019-12-01 00:00:00
abstract：:New high-dimensional, single-cell technologies offer unprecedented resolution in the analysis of heterogeneous tissues. However, because these technologies can measure dozens of parameters simultaneously in individual cells, data interpretation can be challenging. Here we present viSNE, a tool that allows one to map h...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2594
更新日期：2013-06-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-020-0658-1
更新日期：2020-09-01 00:00:00
abstract：:Tocotrienols are the primary form of vitamin E in seeds of most monocot plants, including cereals such as rice and wheat. As potent antioxidants, tocotrienols contribute to the nutritive value of cereal grains in human and livestock diets. cDNAs encoding homogentisic acid geranylgeranyl transferase (HGGT), which catal...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt853
更新日期：2003-09-01 00:00:00
abstract：:RNA interference (RNAi) is a conserved sequence-specific, gene-silencing mechanism that is induced by double-stranded RNA. RNAi holds great promise as a novel nucleic acid-based therapeutic against a wide variety of diseases, including cancer, infectious diseases and genetic disorders. Antiviral RNAi strategies have r...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt1369
更新日期：2007-12-01 00:00:00
abstract：:Selectable marker genes are required to ensure the efficient genetic modification of crops. Economic incentives and safety concerns have prompted the development of several strategies (site-specific recombination, homologous recombination, transposition, and co-transformation) to eliminate these genes from the genome ...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt0602-575
更新日期：2002-06-01 00:00:00
abstract：:We show that transplantation of adult bone marrow-derived cells expressing c-kit reduces hyperglycemia in mice with streptozotocin-induced pancreatic damage. Although quantitative analysis of the pancreas revealed a low frequency of donor insulin-positive cells, these cells were not present at the onset of blood gluco...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt841
更新日期：2003-07-01 00:00:00
abstract：:The Human Proteome Organization (HUPO) recently completed the first large-scale collaborative study to characterize the human serum and plasma proteomes. The study was carried out in different locations and used diverse methods and instruments to compare and integrate tandem mass spectrometry (MS/MS) data on aliquots ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1183
更新日期：2006-03-01 00:00:00
abstract：:Analysis of abnormally methylated genes is increasingly important in basic research and in the development of cancer biomarkers. We have developed methyl-BEAMing technology to enable absolute quantification of the number of methylated molecules in a sample. Individual DNA fragments are amplified and analyzed either by...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1559
更新日期：2009-09-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41587-020-0427-1
更新日期：2020-03-01 00:00:00
abstract：:Purging of tumor cells and selection of stem cells are key technologies for enabling stem cell transplantation and stem cell gene therapy. Here we report a strategy for cell selection based on physical properties of the cells. Exposing cells to an external pulsed electric field (PEF) increases the natural potential di...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/78504
更新日期：2000-08-01 00:00:00
abstract：:Changes in glycosylation are often associated with disease progression, but the genetic and metabolic basis of these events is rarely understood in detail at a molecular level. We describe a metabolism-based approach to the selection of mutants in glycoconjugate biosynthesis that provides insight into regulatory mecha...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/89305
更新日期：2001-06-01 00:00:00
abstract：:We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces ac...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1538
更新日期：2009-05-01 00:00:00
abstract：:Despite recent accomplishments, significant challenges remain before cloning can become a routine part of transgenic animal production. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/80231
更新日期：2000-10-01 00:00:00
abstract：:A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, huma...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0897-759
更新日期：1997-08-01 00:00:00
abstract：:High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequen...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt836
更新日期：2003-07-01 00:00:00
abstract：:Escherichia coli-based artificial chromosomes have become important tools for physical mapping and sequencing in various genome projects. The lack of a general method to modify these large bacterial clones, however, has limited their utility in functional studies. We developed a simple method to modify bacterial artif...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0997-859
更新日期：1997-09-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41587-020-0457-8
更新日期：2020-03-01 00:00:00
abstract：:The functional state of a cell is largely determined by the spatiotemporal organization of its proteome. Technologies exist for measuring particular aspects of protein turnover and localization, but comprehensive analysis of protein dynamics across different scales is possible only by combining several methods. Here w...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2281
更新日期：2012-06-24 00:00:00
abstract：:Academic researchers have regularly ignored patents on key technologies as a strategy to maneuver around patent thickets and freedom-to-operate issues, but they may be more at risk than they realize. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1107-1225
更新日期：2007-11-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-019-0167-2
更新日期：2019-07-01 00:00:00
abstract：:This report describes the selection of highly efficient antibody catalysts by combining chemical selection from a synthetic library with directed in vitro protein evolution. Evolution started from a naive antibody library displayed on phage made from fully synthetic, antibody-encoding genes (the Human Combinatorial An...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt828
更新日期：2003-06-01 00:00:00
abstract：:Heterologous pathways used in metabolic engineering may produce intermediates toxic to the cell. Dynamic control of pathway enzymes could prevent the accumulation of these metabolites, but such a strategy requires sensors, which are largely unknown, that can detect and respond to the metabolite. Here we applied whole-...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2689
更新日期：2013-11-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-019-0292-y
更新日期：2019-11-01 00:00:00
abstract：:Centromeric variation has been linked to cancer and infertility, but centromere sequences contain multiple tandem repeats and can only be assembled manually from long error-prone reads. Here we describe the centroFlye algorithm for centromere assembly using long error-prone reads, and apply it to assemble human centro...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0582-4
更新日期：2020-11-01 00:00:00
abstract：:The site-specific recombinases FLP and Cre are useful for genomic engineering in many living systems. Manipulation of their enzymatic properties offers a means to improve their applicability in different host organisms. We chose to manipulate the thermolability of FLP recombinase. A lacZ-based recombination assay in E...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0798-657
更新日期：1998-07-01 00:00:00
abstract：:Current tools for targeted RNA editing rely on the delivery of exogenous proteins or chemically modified guide RNAs, which may lead to aberrant effector activity, delivery barrier or immunogenicity. Here, we present an approach, called leveraging endogenous ADAR for programmable editing of RNA (LEAPER), that employs s...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0178-z
更新日期：2019-09-01 00:00:00
abstract：:The biology of multicellular organisms is coordinated across multiple size scales, from the subnanoscale of molecules to the macroscale, tissue-wide interconnectivity of cell populations. Here we introduce a method for super-resolution imaging of the multiscale organization of intact tissues. The method, called magnif...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3641
更新日期：2016-09-01 00:00:00
