Challenges in deriving high-confidence protein identifications from data gathered by a HUPO plasma proteome collaborative study.

abstract：:Centromeric variation has been linked to cancer and infertility, but centromere sequences contain multiple tandem repeats and can only be assembled manually from long error-prone reads. Here we describe the centroFlye algorithm for centromere assembly using long error-prone reads, and apply it to assemble human centro...

journal_title：Nature biotechnology

authors： Bzikadze AV,Pevzner PA

更新日期：2020-11-01 00:00:00

abstract：:An approach to the systematic identification and quantification of the proteins contained in the microsomal fraction of cells is described. It consists of three steps: (1) preparation of microsomal fractions from cells or tissues representing different states; (2) covalent tagging of the proteins with isotope-coded af...

journal_title：Nature biotechnology

authors： Han DK,Eng J,Zhou H,Aebersold R

更新日期：2001-10-01 00:00:00

abstract：:Pollen-mediated movement of transgenes from transplastomic oilseed rape (Brassica napus) into wild relatives will be avoided if chloroplasts are maternally transmitted. We assess the probability of chloroplast exchange between conventional oilseed rape and wild Brassica rapa to model the future behavior of transplasto...

journal_title：Nature biotechnology

authors： Scott SE,Wilkinson MJ

更新日期：1999-04-01 00:00:00

abstract：:The exocrine pancreas, liver, and submandibular glands of the rat were used to express and secrete two exogenous, human protein hormones (growth hormone and insulin) into blood at physiological concentrations. Transfection, expression, and secretion were achieved by the in vivo retrograde injection of plasmid DNA into...

journal_title：Nature biotechnology

authors： Goldfine ID,German MS,Tseng HC,Wang J,Bolaffi JL,Chen JW,Olson DC,Rothman SS

更新日期：1997-12-01 00:00:00

abstract：:We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 microm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing mic...

journal_title：Nature biotechnology

authors： Brenner S,Johnson M,Bridgham J,Golda G,Lloyd DH,Johnson D,Luo S,McCurdy S,Foy M,Ewan M,Roth R,George D,Eletr S,Albrecht G,Vermaas E,Williams SR,Moon K,Burcham T,Pallas M,DuBridge RB,Kirchner J,Fearon K,Mao J,

更新日期：2000-06-01 00:00:00

abstract：:We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation ...

journal_title：Nature biotechnology

authors： Kim K,Zhao R,Doi A,Ng K,Unternaehrer J,Cahan P,Huo H,Loh YH,Aryee MJ,Lensch MW,Li H,Collins JJ,Feinberg AP,Daley GQ

更新日期：2011-11-27 00:00:00

abstract：:The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...

journal_title：Nature biotechnology

authors： Giordano-Coltart J,Calkins CW

更新日期：2008-02-01 00:00:00

abstract：:We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...

journal_title：Nature biotechnology

authors： Xiao L,Feng Q,Liang S,Sonne SB,Xia Z,Qiu X,Li X,Long H,Zhang J,Zhang D,Liu C,Fang Z,Chou J,Glanville J,Hao Q,Kotowska D,Colding C,Licht TR,Wu D,Yu J,Sung JJ,Liang Q,Li J,Jia H,Lan Z,Tremaroli V,Dworzyn

更新日期：2015-10-01 00:00:00

abstract：:The unique bread-making characteristic of wheat flour is closely related to the elasticity and extensibility of the gluten proteins stored in the starchy endosperm, particularly the high-molecular-weight glutenin subunits (HMW-GS), which are important in determining gluten and dough elasticity. The quality of wheat cu...

journal_title：Nature biotechnology

authors： Altpeter F,Vasil V,Srivastava V,Vasil IK

更新日期：1996-09-01 00:00:00

abstract：:Monitoring the T cell receptor (TCR) repertoire in health and disease can provide key insights into adaptive immune responses, but the accuracy of current TCR sequencing (TCRseq) methods is unclear. In this study, we systematically compared the results of nine commercial and academic TCRseq methods, including six rapi...

journal_title：Nature biotechnology

authors： Barennes P,Quiniou V,Shugay M,Egorov ES,Davydov AN,Chudakov DM,Uddin I,Ismail M,Oakes T,Chain B,Eugster A,Kashofer K,Rainer PP,Darko S,Ransier A,Douek DC,Klatzmann D,Mariotti-Ferrandiz E

更新日期：2020-09-07 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) that have the potential to elicit new bone in vivo have been used in a tissue-engineering approach for the repair of bone injuries and bone defects. Although it is now possible to generate large amounts of recombinant human (rh) BMPs for medical use, the major challenge remains in th...

journal_title：Nature biotechnology

authors： Saito N,Okada T,Horiuchi H,Murakami N,Takahashi J,Nawata M,Ota H,Nozaki K,Takaoka K

更新日期：2001-04-01 00:00:00

abstract：:Gossypium hirsutum has proven difficult to sequence owing to its complex allotetraploid (AtDt) genome. Here we produce a draft genome using 181-fold paired-end sequences assisted by fivefold BAC-to-BAC sequences and a high-resolution genetic map. In our assembly 88.5% of the 2,173-Mb scaffolds, which cover 89.6%∼96.7%...

journal_title：Nature biotechnology

authors： Li F,Fan G,Lu C,Xiao G,Zou C,Kohel RJ,Ma Z,Shang H,Ma X,Wu J,Liang X,Huang G,Percy RG,Liu K,Yang W,Chen W,Du X,Shi C,Yuan Y,Ye W,Liu X,Zhang X,Liu W,Wei H,Wei S,Huang G,Zhang X,Zhu S,Zhang H,Sun

更新日期：2015-05-01 00:00:00

abstract：:Phosphorus (P) is one of the most important nutrients limiting agricultural production worldwide. In acid and alkaline soils, which make up over 70% of the world's arable land, P forms insoluble compounds that are not available for plant use. To reduce P deficiencies and ensure plant productivity, nearly 30 million to...

journal_title：Nature biotechnology

authors： López-Bucio J,de La Vega OM,Guevara-García A,Herrera-Estrella L

更新日期：2000-04-01 00:00:00

abstract：:We have developed a strategy for improving the stimulation of T cells during immune responses by constructing recombinant antibodies that enhance the delivery of antigen to antigen-presenting cells, such as B cells. These antibodies have variable regions specific for surface molecules on B cells, and a constant region...

journal_title：Nature biotechnology

authors： Lunde E,Munthe LA,Vabø A,Sandlie I,Bogen B

更新日期：1999-07-01 00:00:00

abstract：:Influenza A virus leads to yearly epidemics and sporadic pandemics. Present prophylactic strategies focus on egg-grown, live, attenuated influenza vaccines (LAIVs), in which attenuation is generated by conferring temperature sensitivity onto the virus. Here we describe an alternative approach to attenuating influenza ...

journal_title：Nature biotechnology

authors： Perez JT,Pham AM,Lorini MH,Chua MA,Steel J,tenOever BR

更新日期：2009-06-01 00:00:00

abstract：:One-size-fits-all intravenous delivery of biologics may be giving way to alternate delivery routes that enable safer and more efficient drug administration. Michael Eisenstein reports. ...

journal_title：Nature biotechnology

authors： Eisenstein M

更新日期：2011-02-01 00:00:00

abstract：:Delivery of short interfering RNAs (siRNAs) remains a key challenge in the development of RNA interference (RNAi) therapeutics. A better understanding of the mechanisms of siRNA cellular uptake, intracellular transport and endosomal release could critically contribute to the improvement of delivery methods. Here we mo...

journal_title：Nature biotechnology

authors： Gilleron J,Querbes W,Zeigerer A,Borodovsky A,Marsico G,Schubert U,Manygoats K,Seifert S,Andree C,Stöter M,Epstein-Barash H,Zhang L,Koteliansky V,Fitzgerald K,Fava E,Bickle M,Kalaidzidis Y,Akinc A,Maier M,Zerial M

更新日期：2013-07-01 00:00:00

abstract：:In vitro reprogramming of somatic cells into a pluripotent embryonic stem cell-like state has been achieved through retroviral transduction of murine fibroblasts with Oct4, Sox2, c-myc and Klf4. In these experiments, the rare 'induced pluripotent stem' (iPS) cells were isolated by stringent selection for activation of...

journal_title：Nature biotechnology

authors： Meissner A,Wernig M,Jaenisch R

更新日期：2007-10-01 00:00:00

abstract：:A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, huma...

journal_title：Nature biotechnology

authors： Sultan P,Schechner JS,McNiff JM,Hochman PS,Hughes CC,Lorber MI,Askenase PW,Pober JS

更新日期：1997-08-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Simoni A,Hammond AM,Beaghton AK,Galizi R,Taxiarchi C,Kyrou K,Meacci D,Gribble M,Morselli G,Burt A,Nolan T,Crisanti A

更新日期：2020-09-01 00:00:00

abstract：:After nearly 40 years of development, oligonucleotide therapeutics are nearing meaningful clinical productivity. One of the key advantages of oligonucleotide drugs is that their delivery and potency are derived primarily from the chemical structure of the oligonucleotide whereas their target is defined by the base seq...

journal_title：Nature biotechnology

authors： Khvorova A,Watts JK

更新日期：2017-03-01 00:00:00

abstract：:Relatively low levels of expression from naturally occurring promoters have limited the use of muscle as a gene therapy target. Myogenic restricted gene promoters display complex organization usually involving combinations of several myogenic regulatory elements. By random assembly of E-box, MEF-2, TEF-1, and SRE site...

journal_title：Nature biotechnology

authors： Li X,Eastman EM,Schwartz RJ,Draghia-Akli R

更新日期：1999-03-01 00:00:00

abstract：:Embryonic stem (ES) cells are a promising source of cardiomyocytes, but clinical application of ES cells has been hindered by the lack of reliable selective differentiation methods. Differentiation into any lineage is partly dependent on the regulatory mechanisms of normal early development. Although several signals, ...

journal_title：Nature biotechnology

authors： Yuasa S,Itabashi Y,Koshimizu U,Tanaka T,Sugimura K,Kinoshita M,Hattori F,Fukami S,Shimazaki T,Ogawa S,Okano H,Fukuda K

更新日期：2005-05-01 00:00:00

abstract：:Many promising targets for T-cell-based cancer immunotherapies are self-antigens. During thymic selection, T cells bearing T cell receptors (TCRs) with high affinity for self-antigen are eliminated. The affinity of the remaining low-avidity TCRs can be improved to increase their antitumor efficacy, but conventional sa...

journal_title：Nature biotechnology

authors： Schmitt TM,Aggen DH,Ishida-Tsubota K,Ochsenreither S,Kranz DM,Greenberg PD

更新日期：2017-12-01 00:00:00

abstract：:In the version of this article initially published, a GlaxoSmithKline compound referred to as GSK298559 was listed in Table 1 as a RIPK1 inhibitor in phase 1 trials for inflammatory bowel disease. The correct designation is GSK2983559; however, this compound is a RIPK2 inhibitor and thus should not have been included ...

journal_title：Nature biotechnology

authors： Sheridan C

更新日期：2019-04-01 00:00:00

abstract：:The specificity of biological regulatory mechanisms relies on selective interactions between different proteins in different cell types and in response to different extracellular signals. We describe a bimolecular fluorescence complementation (BiFC) approach for the simultaneous visualization of multiple protein inter...

journal_title：Nature biotechnology

authors： Hu CD,Kerppola TK

更新日期：2003-05-01 00:00:00

abstract：:We describe a simple technology used to cure an established metastatic disease. Intradermal injection of plasmid DNA encoding a transcriptionally targeted cytotoxic gene, along with hsp70, not only promoted tissue-specific, inflammatory killing of normal melanocytes, but also induced a CD8(+) T-cell-dependent, antigen...

journal_title：Nature biotechnology

authors： Daniels GA,Sanchez-Perez L,Diaz RM,Kottke T,Thompson J,Lai M,Gough M,Karim M,Bushell A,Chong H,Melcher A,Harrington K,Vile RG

更新日期：2004-09-01 00:00:00

abstract：:Expansions of short tandem repeats are genetic variants that have been implicated in several neuropsychiatric and other disorders, but their assessment remains challenging with current polymerase-based methods1-4. Here we introduce a CRISPR-Cas-based enrichment strategy for nanopore sequencing combined with an algorit...

journal_title：Nature biotechnology

authors： Giesselmann P,Brändl B,Raimondeau E,Bowen R,Rohrandt C,Tandon R,Kretzmer H,Assum G,Galonska C,Siebert R,Ammerpohl O,Heron A,Schneider SA,Ladewig J,Koch P,Schuldt BM,Graham JE,Meissner A,Müller FJ

更新日期：2019-12-01 00:00:00

abstract：:Small-interfering RNAs (siRNAs) assemble into RISC, the RNA-induced silencing complex, which cleaves complementary mRNAs. Despite their fluctuating efficacy, siRNAs are widely used to assess gene function. Although this limitation could be ascribed, in part, to variations in the assembly and activation of RISC, downst...

journal_title：Nature biotechnology

authors： Tafer H,Ameres SL,Obernosterer G,Gebeshuber CA,Schroeder R,Martinez J,Hofacker IL

更新日期：2008-05-01 00:00:00

abstract：:Functional genomics and proteomics are identifying many potential drug targets for novel therapeutic proteins, and both rational and combinatorial protein engineering methods are available for creating drug candidates. A central challenge is the definition of the most appropriate design criteria, which will benefit cr...

journal_title：Nature biotechnology

authors： Rao BM,Lauffenburger DA,Wittrup KD

更新日期：2005-02-01 00:00:00