Abstract:
:One-size-fits-all intravenous delivery of biologics may be giving way to alternate delivery routes that enable safer and more efficient drug administration. Michael Eisenstein reports.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Eisenstein Mdoi
10.1038/nbt.1768subject
Has Abstractpub_date
2011-02-01 00:00:00pages
107-9issue
2eissn
1087-0156issn
1546-1696pii
nbt.1768journal_volume
29pub_type
新闻abstract::Isolating antigen-specific T lymphocytes is hampered by the low frequency of the cells and the low affinity between T-cell receptors (TCR) and antigen. We describe the isolation and purification of antigen-specific CD8+ T lymphocytes from mixed T-cell populations. Magnetic beads coated with major histocompatibility co...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0398-281
更新日期:1998-03-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-019-0320-y
更新日期:2019-12-01 00:00:00
abstract::In vitro reprogramming of somatic cells into a pluripotent embryonic stem cell-like state has been achieved through retroviral transduction of murine fibroblasts with Oct4, Sox2, c-myc and Klf4. In these experiments, the rare 'induced pluripotent stem' (iPS) cells were isolated by stringent selection for activation of...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1335
更新日期:2007-10-01 00:00:00
abstract::Multiple intravenous injections of a cDNA library, derived from human melanoma cell lines and expressed using the highly immunogenic vector vesicular stomatitis virus (VSV), cured mice with established melanoma tumors. Successful tumor eradication was associated with the ability of mouse lymphoid cells to mount a tumo...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2157
更新日期:2012-03-18 00:00:00
abstract::After nearly 40 years of development, oligonucleotide therapeutics are nearing meaningful clinical productivity. One of the key advantages of oligonucleotide drugs is that their delivery and potency are derived primarily from the chemical structure of the oligonucleotide whereas their target is defined by the base seq...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt.3765
更新日期:2017-03-01 00:00:00
abstract::The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies and then labeled the immunocomplexes (one or zero labeled target pr...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1641
更新日期:2010-06-01 00:00:00
abstract::Plant satellite RNAs generally reduce the level of helper virus accumulation and attenuate the disease symptoms induced by the helper virus that they depend upon for replication and packaging. As such, satellite RNAs could be used as biocontrol agents to reduce the level of disease in field crops, either by the applic...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1096-1264
更新日期:1996-10-01 00:00:00
abstract::Current base editors (BEs) catalyze only base transitions (C to T and A to G) and cannot produce base transversions. Here we present BEs that cause C-to-A transversions in Escherichia coli and C-to-G transversions in mammalian cells. These glycosylase base editors (GBEs) consist of a Cas9 nickase, a cytidine deaminase...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0592-2
更新日期:2021-01-01 00:00:00
abstract::Tissue function depends on hierarchical structures extending from single cells ( approximately 10 microm) to functional subunits (100 microm-1 mm) that coordinate organ functions. Conventional cell culture disperses tissues into single cells while neglecting higher-order processes. The application of semiconductor-dri...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1361
更新日期:2008-01-01 00:00:00
abstract::Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1520
更新日期:2009-02-01 00:00:00
abstract::Methods used to sequence the transcriptome often produce more than 200 million short sequences. We introduce StringTie, a computational method that applies a network flow algorithm originally developed in optimization theory, together with optional de novo assembly, to assemble these complex data sets into transcripts...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3122
更新日期:2015-03-01 00:00:00
abstract::Can new approaches for deriving human stem cells circumnavigate existing patents that dominate embryonic stem cell intellectual property? ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0406-411
更新日期:2006-04-01 00:00:00
abstract::The interaction between the HSP90 chaperone and its client kinases is sensitive to the conformational status of the kinase, and stabilization of the kinase fold by small molecules strongly decreases chaperone interaction. Here we exploit this observation and assay small-molecule binding to kinases in living cells, usi...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2620
更新日期:2013-07-01 00:00:00
abstract::Correct reconstruction of macromolecular structure by cryo-electron microscopy (cryo-EM) relies on accurate determination of the orientation of single-particle images. For small (<100 kDa) DNA-binding proteins, obtaining particle images with sufficiently asymmetric features to correctly guide alignment is challenging....
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0716-8
更新日期:2020-10-19 00:00:00
abstract::The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing G bases. Here we report the evolution of three new SpCas9 variants that collectively recognize NRNH PAMs (where R is A or G and H is A, C or T) using ph...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0412-8
更新日期:2020-04-01 00:00:00
abstract::Noncoding RNAs (ncRNAs) are emerging as key molecules in human cancer, with the potential to serve as novel markers of disease and to reveal uncharacterized aspects of tumor biology. Here we discover 121 unannotated prostate cancer-associated ncRNA transcripts (PCATs) by ab initio assembly of high-throughput sequencin...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1914
更新日期:2011-07-31 00:00:00
abstract::A central hurdle in developing small interfering RNAs (siRNAs) as therapeutics is the inefficiency of their delivery across the plasma and endosomal membranes to the cytosol, where they interact with the RNA interference machinery. With the aim of improving endosomal release, a poorly understood and inefficient proces...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3298
更新日期:2015-08-01 00:00:00
abstract::Although mass spectrometry is well suited to identifying thousands of potential protein post-translational modifications (PTMs), it has historically been biased towards just a few. To measure the entire set of PTMs across diverse proteomes, software must overcome the dual challenges of covering enormous search spaces ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0067-5
更新日期:2019-04-01 00:00:00
abstract::Circular RNA transcripts were first identified in the early 1990s but knowledge of these species has remained limited, as their study through traditional methods of RNA analysis has been difficult. Now, novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have allowed compr...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2890
更新日期:2014-05-01 00:00:00
abstract::Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt.4022
更新日期:2017-12-01 00:00:00
abstract::As the cost of human genome sequencing plunges and large-scale genome-phenotype studies become possible, society should do more to reward those individuals who choose to disclose their data, despite the risks. ...
journal_title:Nature biotechnology
pub_type: 评论,社论
doi:10.1038/nbt0909-777
更新日期:2009-09-01 00:00:00
abstract::DNA charge transport chemistry is found to provide a sensitive method for probing protein-dependent changes in DNA structure and enzymatic reactions. Here we describe the development of an electrochemical assay of protein binding to DNA-modified electrodes based upon the detection of associated perturbations in DNA ba...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0302-282
更新日期:2002-03-01 00:00:00
abstract::The oncofetal fibronectin (B-FN) isoform is present in vessels of neoplastic tissues during angiogenesis but not in mature vessels. B-FN could therefore provide a target for diagnostic imaging and therapy of cancer. Phage display libraries have been used to isolate human antibody fragments with pan-species recognition...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1197-1271
更新日期:1997-11-01 00:00:00
abstract::In the version of this article initially published, a GlaxoSmithKline compound referred to as GSK298559 was listed in Table 1 as a RIPK1 inhibitor in phase 1 trials for inflammatory bowel disease. The correct designation is GSK2983559; however, this compound is a RIPK2 inhibitor and thus should not have been included ...
journal_title:Nature biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41587-019-0082-6
更新日期:2019-04-01 00:00:00
abstract::Artificial transcription factors containing designer zinc-finger DNA-binding domains (DBDs) have been used to activate or repress expression of a growing number of endogenous genes. We have combined targeted zinc-finger DBD technology with a dimerizer-regulated gene expression system to permit the small-molecule contr...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0702-729
更新日期:2002-07-01 00:00:00
abstract::Systemic gene delivery into muscle has been a major challenge for muscular dystrophy gene therapy, with capillary blood vessels posing the principle barrier and limiting vector dissemination. Previous efforts to deliver genes into multiple muscles have relied on isolated vessel perfusion or pharmacological interventio...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1073
更新日期:2005-03-01 00:00:00
abstract::A major shortcoming to the use of adeno-associated virus (rAAV) vectors is their limited packaging size. To overcome this hurdle, we split an expression cassette and cloned it into two separate vectors. The vectors contained either a nuclear localizing Escherichia coli lacZ transgene (nlslacZ) with a splice acceptor, ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/75390
更新日期:2000-05-01 00:00:00
abstract::As biotechnology advances, the day may soon come for the creation of a self-aware, human-nonhuman chimera. The USPTO has ruled on whether a patent may issue on such an organism, but Congress must still legislate a dividing line between human and non-human patentable subject matter. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0506-517
更新日期:2006-05-01 00:00:00
abstract::The clinical translation of cationic α-helical antimicrobial peptides (AMPs) has been hindered by structural instability, proteolytic degradation and in vivo toxicity from nonspecific membrane lysis. Although analyses of hydrophobic content and charge distribution have informed the design of synthetic AMPs with increa...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0222-z
更新日期:2019-10-01 00:00:00
abstract::Tocotrienols are the primary form of vitamin E in seeds of most monocot plants, including cereals such as rice and wheat. As potent antioxidants, tocotrienols contribute to the nutritive value of cereal grains in human and livestock diets. cDNAs encoding homogentisic acid geranylgeranyl transferase (HGGT), which catal...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt853
更新日期:2003-09-01 00:00:00